Food Bioscience最新文献

{"title":"Transcriptomics and metabolomics provide the difference of unique odor between natural and cultivated Ophiocordyceps sinensis and the biosynthetic pathway of key volatile compounds","authors":"Tao Wang ,&nbsp;Mengjun Xiao ,&nbsp;Chuyu Tang ,&nbsp;Jianzhao Qi ,&nbsp;Yuling Li ,&nbsp;Xiuzhang Li","doi":"10.1016/j.fbio.2025.106200","DOIUrl":"10.1016/j.fbio.2025.106200","url":null,"abstract":"<div><div><em>Ophiocordyceps sinensis</em> has a unique fishy odor, but its chemical composition and biosynthesis process have not been paid attention to. In this study, HS-SPME-GC/MS and transcriptome were integrated to explore the differences in volatile compounds (VOCs) and their formation mechanisms between natural and cultivated <em>O. sinensis</em>. We qualitatively and quantitatively analyzed the VOCs in natural and cultivated <em>O. sinensis</em>, and identified 17 key volatile compounds (KOCs) by multivariate statistical analysis combined with relative odor activity (ROVA) value. They identified similar but heterogeneously accumulated fishy odor characteristics in natural and cultivated <em>O. sinensis</em> and their different tissues. Among them, 10 KOCs such as acetic acid, 2-ethylhexyl ester, 1-butanol, 3-methyl-propanoate, 2-n-butyl furan, 2-octanone, 2-butanone,4-(2,6,6-trimethyl-2-cyclohexen-1-yl), 3-mercapto-3-methylbutanol, 1-octen-3-ol, benzeneacetaldehyde, p-cymene, β-damascone and naphthalene, 2-methyl were abundantly enriched in natural <em>O. sinensis</em>, forming woody, balsamic and mushroom-like woody aromas. In cultivated <em>O. sinensis</em>, higher levels of styrene, isophorone and 1h-pyrrole, 1-(2-furanylmethyl) (3 KOCs) form fruity, floral and berry aromas and sweetness similar to honey and rose. These KOCs are more enriched in sclerotia, which means that sclerotia contributes more to the overall odor than stroma. Transcriptome analysis showed that the heterogeneous accumulation of metabolic flux in monoterpenoid biosynthesis and sesquiterpenoid and triterpenoid biosynthesis regulated by core genes such as <em>Zn_clus</em>, <em>psiD</em>, <em>Gpd-2</em> and <em>chol-2</em> in the sample explained the biosynthesis process of fishy gas. These results reveal the chemical composition and biosynthesis basis of <em>O. sinensis</em> odor, expand our understanding of the synthesis process of filamentous fungi odor substances, and provide a new strategy for rapid identification of natural and cultivated <em>O. sinensis</em>.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106200"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and physical characterization, stability and antioxidant activity of avicularin/(2-hydroxypropyl)-β-cyclodextrin inclusion complex","authors":"Xiaoai Zhu ,&nbsp;Yong Cao ,&nbsp;Qianchun Deng ,&nbsp;Xia Xiang ,&nbsp;Jun Xi ,&nbsp;Konglong Feng ,&nbsp;Zili Gao ,&nbsp;Kunlun Liu ,&nbsp;Hang Xiao","doi":"10.1016/j.fbio.2025.106201","DOIUrl":"10.1016/j.fbio.2025.106201","url":null,"abstract":"<div><div>Avicularin, commonly found in apples and mangos, possessed various health-promoting effects. However, the application of avicularin in functional foods was hindered by its limited water solubility and chemical instability. A freeze-drying-based technique was employed to formulate a complex using avicularin and (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) to improve these properties. The synthesis and structure of the complex were featured by changes in fourier transform infrared spectroscopy (FT-IR), the phase transition recorded by differential scanning calorimetry (DSC), the changes in crystal structures provided by X-ray diffractometry (XRD), the visualized changes in surface morphology determined by scanning electron microscopy (SEM). Additionally, proton nuclear magnetic resonance spectroscopy (¹H NMR) analysis and molecular docking simulations demonstrated the A ring of avicularin entered into the cavity of HP-β-CD to form a supramolecular complex. The complex of avicularin resulted in its increased water solubility (22 times), and enhanced chemical stability against heat and alkaline conditions, especially at pH 9,10, with an improvement of up to 50%. Meanwhile, the inclusion complex improved antioxidant activity in the cellular assays, especially at 120 μg/mL, with an improvement of up to 35%. This work provided a solid basis for potential applications of avicularin in functional foods and nutraceuticals.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106201"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative manufacturing process to enhance the taste and color of Ancha tea: A comprehensive analysis using sensory evaluation, chemometrics, and metabolomics","authors":"Zhenbin Chen ,&nbsp;Yanqun Jiang ,&nbsp;Zhibin Ye ,&nbsp;Wei Zhao ,&nbsp;Jixin Zhang ,&nbsp;Wenjing Huang ,&nbsp;Yida Wu ,&nbsp;Tiehan Li ,&nbsp;Qian Xu ,&nbsp;Jingming Ning","doi":"10.1016/j.fbio.2025.106208","DOIUrl":"10.1016/j.fbio.2025.106208","url":null,"abstract":"<div><div><em>Ancha</em> tea (ACT) is a type of dark tea processed through dew wetting. However, the unpredictability and instability of the dew wetting process limits the efficiency and quality of production. To address this issue, we used the same primary tea to produce no dew wetting ACT (DDW), dew wetting ACT (DW), and three new process ACT (SF5, SF8, and SF10). The results showed that SF8 had the best quality among all the samples; moreover, the color and taste of DW were superior to DDW. Using LC-Orbitrap-MS analysis, we identified 48 differential metabolites. Through partial least squares regression (PLSR) and correlation analysis, we found that five components, including quercetin 3-galactoside, chlorogenic acid, and kaempferol, contributed the most to the infusion color, while fifteen components were identified as the major contributors to taste. Further metabolic pathway analysis revealed that the new process promoted the degradation of epigallocatechin gallate and quercetin 3-galactoside and increased the transformation of <em>D</em>-glucose and <em>L</em>-pyroglutamic acid, possibly through the moist-heat effect, resulting in improved taste. These transformations occur at a lower rate during dew wetting. This study proposes a new process and compares its effects and mechanisms on the quality of ACT with that produced using dew wetting process. The findings provide a solution to the problems of low efficiency, unstable product quality, and poorer quality of fresh tea in ACT production, and promotes standardized and high-quality production of ACT.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106208"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of whey protein types on the bioaccessibility of β-carotene in different formulations: Emulsions vs microcapsules","authors":"Shanshan Hu ,&nbsp;Fang Tian ,&nbsp;Shiqi Yi ,&nbsp;Qihang Liu ,&nbsp;Shilin Liu ,&nbsp;Yanrong Zhao ,&nbsp;Xuguang Zhang ,&nbsp;Yan Li","doi":"10.1016/j.fbio.2025.106173","DOIUrl":"10.1016/j.fbio.2025.106173","url":null,"abstract":"<div><div>The processing on whey protein has been concerned due to its high nutritional values and allergenicity. However, how different whey proteins will affect the absorption efficiency of other nutraceuticals in the formula is still unclear. Hence, four commercial whey proteins, namely whey protein concentrate (WPC), whey protein rich in globular-membrane phospholipids (MPL), moderately hydrolyzed whey protein (MHP) and highly hydrolyzed whey protein (HHP), were selected as the stabilizer to prepare emulsions, which were then spray-dried into microcapsules. β-carotene was selected as the representative lipophilic ingredient to study the effect of whey proteins on its bioaccessibility using an <em>in vitro</em> digestion model. The results indicated that the average particle size of WPC, MPL, and MHP emulsions is approximately 140 nm, while that of HHP emulsion had particle size of 16 μm. The micro-scaled microcapsules stabilized by MHP were superior to others stabilized by WPC, MPL, HHP in dispersibility and encapsulation characteristics. During digestion, MPL and MHP reduced the aggregation behavior of emulsion particles in the stomach. Microcapsules demonstrated superior bioaccessibility of β-carotene by 1.49–1.66 times compared to emulsions, while MPL emulsions (51.89 ± 2.94%) and microcapsules (77.50 ± 0.78%) exhibited the highest β-carotene bioaccessibility, increased by 1.03–1.69 and 1.12–1.22 times, respectively, as compared to other proteins. These findings offer valuable insights into the additional benefit of different whey proteins in enhancing the absorption of lipophilic nutrients in formula milk.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106173"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding nattokinase efficacy: From digestion and absorption to lipid pathway modulation in high-fat diet-induced atherosclerosis","authors":"Yongjie Zhou ,&nbsp;Zixin Fu ,&nbsp;Peipei Dou ,&nbsp;Chen Yu ,&nbsp;Xianyou Dong ,&nbsp;Hui Hong ,&nbsp;Yongkang Luo ,&nbsp;Yuqing Tan","doi":"10.1016/j.fbio.2025.106203","DOIUrl":"10.1016/j.fbio.2025.106203","url":null,"abstract":"<div><div>Nattokinase (NK), an alkaline serine protease, is recognized for its cardiovascular benefits through the modulation of blood coagulation and fibrinolytic pathways. Atherosclerosis (AS), a primary cause of cardiovascular disease (CVD) worldwide, is responsive to dietary interventions. However, studies investigating NK's digestion, absorption, and mechanisms in mitigating AS remain limited. This study evaluated the effects of high-dose NK treatment (H-NK, 900 FU/kg body weight) administered over six weeks in ApoE^−/− mice, which resulted in significant reductions in atherosclerotic lesions. <em>In vitro</em> digestion simulations showed that 25.8% of NK's enzymatic activity was retained post-digestion, and thrombolysis assays demonstrated a thrombolytic efficacy of 30–40% at concentrations ≥5000 μg/mL, regardless of clot age. Absorption experiments using a rat everted intestinal sac model revealed a small intestine absorption rate of 37.0%, with the absorbed peptides being predominantly hydrophobic, uncharged, and shorter than 10 amino acids. Proteomic analysis of mouse liver tissues identified that high-dose NK down-regulated PXDN (peroxidasin) and PNLIP (pancreatic lipase), proteins involved in promoting lipid oxidation, and intestinal lipid absorption. These proteomic changes enhanced lipid metabolism, reduced lipid peroxidation, and alleviated AS through the \"Lipid and Atherosclerosis\" pathway. Overall, this study provides a deeper understanding of NK's biological activity and supports its potential as a novel dietary supplement for the management of CVD.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106203"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal properties and preservation applications of resin acid modified chitosan derivatives","authors":"Zhuo Xu ,&nbsp;Bin Wu ,&nbsp;Chunhua Wu ,&nbsp;Qian Chen ,&nbsp;Kaimeng Xu ,&nbsp;Zhengjun Shi ,&nbsp;Xiaoping Rao ,&nbsp;Yanfei Niu","doi":"10.1016/j.fbio.2025.106204","DOIUrl":"10.1016/j.fbio.2025.106204","url":null,"abstract":"<div><div>In order to obtain natural bio-antimicrobial agents with better antifungal activity, the present study compared the antifungal properties of different structural natural resin acid derivatives (dehydroabietic acid (DHA), acrylpimaric acid (APA), and maleopimaric anhydride (MPA)) grafted with chitosan C₂-OH and investigated the relationship between structure and activity. The structures of the derivatives were determined by FTIR and ¹H NMR. The antifungal properties were determined by mycelial growth rate method. The results demonstrated that CS-DHA (CSDA) exhibited the capacity to inhibit the majority of the tested fungi with notable efficacy, with an optimal antifungal effect observed at 97.53% against <em>Alternaria solani</em>. CS-APA (CSAA) demonstrated a comparatively weaker antifungal effect in comparison to CSDA, yet it exhibited a stronger effect when compared to CS-MPA (CSMA). SEM, TEM and relative permeability of cell membranes proved that the derivatives inhibited fungal growth by destroying the cell wall structure. The <em>in vivo</em> experiments proved that the derivatives all have the effect of protecting chili peppers, which has some application prospects. The best curative effect of CSAA on chili was observed at 1.5 mg/mL concentration (21.04%). At 3 mg/mL concentration, CSDA showed the best protective effect (29.48%) against chili peppers, which was closest to the effect of Imazalil at the same concentration. Meanwhile, the three derivatives had good preservation effect on cherry tomatoes, and the longest one could extend the shelf life by 6 days.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106204"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dihydromyricetin as a dietary supplement enhances hypoglycemic efficacy of Sangzhi Alkaloids via gut-liver axis","authors":"Yuwen Guan ,&nbsp;Qiuyue Lv ,&nbsp;Xinyu Wang ,&nbsp;Baoping Jiang ,&nbsp;Peigen Xiao","doi":"10.1016/j.fbio.2025.106197","DOIUrl":"10.1016/j.fbio.2025.106197","url":null,"abstract":"<div><div>Chronic hyperglycemia, a hallmark of Type 2 diabetes mellitus, presents significant challenges in long-term management due to drug resistance and adverse effects. Dihydromyricetin (DHM), a natural flavanol from vine tea (<em>Ampelopsis grossedentata</em>), widely recognized as a nutraceutical, shows potential in enhancing the efficacy of pharmacological therapies. This study investigates the role of DHM in improving the hypoglycemic effects of Sangzhi Alkaloids (SZ-A), a commercially used glucosidase inhibitor. Our results demonstrate that DHM supplementation allows for a reduction in SZ-A dosage while maintaining or surpassing its glucose-lowering efficacy, thereby mitigating gastrointestinal side effects. Mechanistic studies reveal that this combination modulates the gut-liver axis, reducing ER stress, and enhancing gut microbiota diversity, including increased beneficial bacteria and non-12OH bile acids. These findings highlight DHM's potential as an effective adjunctive nutraceutical, offering a safer and more efficient therapeutic strategy for managing hyperglycemia and related metabolic disorders.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106197"},"PeriodicalIF":4.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the anticancer potential of Dianthus orientalis in pancreatic cancer: A molecular and cellular study","authors":"Mehmet Aykut Yildirim ,&nbsp;Burak Sevinc ,&nbsp;Samet Paydas ,&nbsp;Mehmet Ali Karaselek ,&nbsp;Tugce Duran ,&nbsp;Serkan Kuccukturk ,&nbsp;Hakan Vatansev ,&nbsp;Mehmet Veysi Cetiz ,&nbsp;Giovanni Caprioli ,&nbsp;Laura Acquaticci ,&nbsp;Ugur Cakilcioglu ,&nbsp;Gokhan Zengin","doi":"10.1016/j.fbio.2025.106183","DOIUrl":"10.1016/j.fbio.2025.106183","url":null,"abstract":"<div><div>Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies, characterized by limited therapeutic options and poor prognosis. This study investigated the anticancer effects of <em>Dianthus orientalis</em> extracts on pancreatic cancer cell lines (Panc-1 and BxPC-3) with a focus on apoptosis, invasion, and metastasis-related pathways. Chemical analysis using HPLC-MS/MS identified bioactive compounds (vanillic acid, syringic acid, p-coumaric acid, quercetin, hyperoxide) known for their anticancer properties. The Inhibitory Concentration 50 (IC₅₀) value, which is half maximal inhibitory concentration of cancer cells, was determined to be 250 μg/mL at 48 h, and significantly induced apoptosis by decreasing anti-apoptotic gene expressions (<em>Bcl-XL</em> and <em>Bcl-2)</em> and increasing pro-apoptotic gene expressions (<em>Casp3</em> and <em>Bax</em>) particularly in the Panc-1 cell line. Flow cytometric analysis revealed approximately 55% apoptosis in both cell lines. Molecular docking and dynamics analyses demonstrated strong binding affinities of quercetin and hyperoxide with important cancer-related proteins, including CHEK2 and PALB2. Gene expression analysis demonstrated the upregulation of pro-apoptotic markers like <em>Bax</em> and <em>Casp3</em>, and selective modulation of metastasis-related genes such as <em>SNAI1</em>, <em>PALB2</em>, and <em>CHEK2</em>. The findings highlight the potential of <em>D. orientalis</em> to modulate DNA repair mechanisms and epithelial-mesenchymal transition (EMT) pathways. Furthermore, network pharmacology and <em>in silico</em> tools corroborated its interactions with critical signaling pathways in cancer progression, including PI3K-Akt and MAPK. The study provides the first comprehensive evidence of <em>D. orientalis's</em> antiproliferative through apoptosis induction on pancreatic cancer cell lines, laying the groundwork for future therapeutic applications.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106183"},"PeriodicalIF":4.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary pyridoxine promoted muscle protein deposition relating to PI3K/AKT/TOR pathway and the ubiquitin proteasome system in grass carp (Ctenopharyngodon idella)","authors":"Li-Fu Gong ,&nbsp;Xiao-Qiu Zhou ,&nbsp;Lin Feng ,&nbsp;Pei Wu ,&nbsp;Wei-Dan Jiang ,&nbsp;Yao-Bin Ma ,&nbsp;Xiao-Wan Jin ,&nbsp;Hong-Mei Ren ,&nbsp;Jia-Yong Tang ,&nbsp;Fa-Li Wu ,&nbsp;Yang Liu","doi":"10.1016/j.fbio.2025.106198","DOIUrl":"10.1016/j.fbio.2025.106198","url":null,"abstract":"<div><div>Pyridoxine (PN) is essential for the normal growth and development of the body. The purpose of this study was to investigate the possible mechanism of pyridoxine in improving the meat quality of grass carp (<em>Ctenopharyngodon idella</em>). A total of 450 grass carp (746.08 ± 1.70 g) were randomly assigned to six treatments, each with three replicates, and fed diets containing pyridoxine 0.32, 1.60, 3.14, 4.59, 6.15 and 7.62 mg/kg, respectively, for 8 weeks. The results showed that the PWG, SGR and FE were increased, indicating that pyridoxine promoted the growth of grass carp. Appropriate pyridoxine increased the crude protein, crude lipid, hydrolyzed amino acids, amino acid score, resilience, hardness, and pH of muscles, indicating that pyridoxine elevated muscle quality. Optimal pyridoxine enhanced the levels of muscle <em>SLC38A2</em>, <em>SLC7A1</em>, <em>IGF-1</em>, <em>PDK1</em> and <em>AKT</em>, p-S6K1, p-TOR, p-AKT and IRS1, and decreased the expression of <em>SLC1A3</em>, <em>PTEN</em>, <em>4EBP1</em> and <em>GSK3β</em>, revealing that pyridoxine may promote protein synthesis by activating the PI3K/AKT/TOR pathway. In addition, the levels of muscle <em>FOXO1a</em>, <em>MuRF1</em>, <em>MAFbx</em>, <em>ULK1</em>, <em>ubiquitin</em>, <em>psmc1</em>, <em>psma2</em>, <em>nrdp1</em>, <em>chip</em>, parkin, and p-FOXO3a were significantly reduced, suggesting that pyridoxine may inhibit protein degradation by preventing protein ubiquitination and autophagy. At last, the pyridoxine requirements of grass carp were determined to be 4.00, 5.09 and 4.96 mg/kg diet, respectively, based on PWG, liver PN content, and muscle GPT activity.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106198"},"PeriodicalIF":4.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum T34 alleviates constipation by enhancing intestinal barrier and modulating gut homeostasis","authors":"Fei Shen ,&nbsp;Huiting Zhu ,&nbsp;Zhongyan Bao ,&nbsp;Di Han ,&nbsp;Juan Du ,&nbsp;Minjie Zhao ,&nbsp;Fengqin Feng ,&nbsp;Guanghua He ,&nbsp;Qiufen Mo","doi":"10.1016/j.fbio.2025.106195","DOIUrl":"10.1016/j.fbio.2025.106195","url":null,"abstract":"<div><div>Constipation is a common gastrointestinal condition. <em>Lactiplantibacillus plantarum</em> T34 has been demonstrated efficacy in relieving loperamide-induced constipation in mice model. Oral intervention of <em>L. plantarum</em> T34 (1 × 10⁸ CFU/mouse/day) resulted in improved defecation and enhanced intestinal morphology. Mechanistic studies revealed that <em>L. plantarum</em> T34 strengthend the intestinal barrier through the upregulation of tight junction protein genes, specifically <em>Claudin-1</em>, as well as mucosal barrier proteins such as <em>Muc2</em> and <em>Muc3</em>. Additionally, <em>L. plantarum</em> T34 modulated gut homeostasis by influencing the expression of aquaporin genes, levels of the gut neurotransmitter serotonin (5-HT), and oxidative stress markers. Furthermore, <em>L. plantarum</em> T34 effectively restored the composition of the gastrointestinal microbiota, addressing dysbiosis linked to constipation. These findings suggested that <em>L. plantarum</em> T34 may serve as a promising probiotic intervention for managing constipation, offering a safe and effective alternative to traditional treatments.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106195"},"PeriodicalIF":4.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}