Food Bioscience最新文献_第9页

Yeast extract-mediated bidirectional regulation of off-odor binding to myofibrillar protein: Mechanisms for off-odor control in surimi products 酵母提取物介导的恶臭与肌纤维蛋白结合的双向调节：鱼糜产品的恶臭控制机制

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1016/j.fbio.2026.108403

Dan Wu , Zhongyang Ren , Wudan Cai , Jian Xiong , Ku Li , Pei Li , Yan Zhang , Qilin Huang

{"title":"Yeast extract-mediated bidirectional regulation of off-odor binding to myofibrillar protein: Mechanisms for off-odor control in surimi products","authors":"Dan Wu , Zhongyang Ren , Wudan Cai , Jian Xiong , Ku Li , Pei Li , Yan Zhang , Qilin Huang","doi":"10.1016/j.fbio.2026.108403","DOIUrl":"10.1016/j.fbio.2026.108403","url":null,"abstract":"<div><div>Controlling off-odor is crucial for improving the sensory quality and consumer acceptance of surimi products. In this study, yeast extract (YE) was innovatively applied to regulate/control off-odor release in surimi products. The changes in structure and off-odor binding capacity of myofibrillar protein (MP) with 0%–2 % YE were monitored during two-stage heating. Notably, MP structural changes in MP induced by YE affected its binding capacity to off-odors, showing a bidirectional regulating effect, i.e., an enhanced capacity at low addition (0.5%), whereas a weakened capacity at high addition (2%). The low YE addition promoted protein unfolding to increase binding sites number and reinforce binding forces (hydrophobic interactions, electrostatic interactions and hydrogen bonds) between MP and off-odors, thus inhibiting release of off-odors. Whereas, excessive YE causes its own aroma compounds to compete with off-odor compounds for binding sites on MP, thus contributing to release off-odors during processing procedure. Molecular dynamics simulations illustrated that L-Arg in YE engaged acidic amino acid residues on MHC in MP through electrostatic interactions, while L-Glu abundant in YE interacted with Arg, Tyr, and Ala residues on MHC via electrostatic, ion-dipole, and hydrogen bonding, thereby inducing structure-function shifts on MP. Molecular docking further revealed that MHC structure change induced by L-Arg and L-Glu in YE affected its binding to off-odor compounds. This study elucidated the bidirectional modulation effects and mechanism of YE on modifying off-odors binding capacity and revealed key role of charged amino acids in YE, offering a clean-label tool for off-odor control in surimi processing.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108403"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of optimal printability properties of personalized mucoadhesive oral films suitable for extrusion-based 3D food printing depending on temperature 适合于基于挤出的3D食品打印的个性化粘接口腔薄膜的最佳打印性能的开发

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-02-03 DOI: 10.1016/j.fbio.2026.108404

Ji Young Yu

{"title":"Development of optimal printability properties of personalized mucoadhesive oral films suitable for extrusion-based 3D food printing depending on temperature","authors":"Ji Young Yu","doi":"10.1016/j.fbio.2026.108404","DOIUrl":"10.1016/j.fbio.2026.108404","url":null,"abstract":"<div><div>Mucoadhesive oral films (MOFs) fabricated by embedding-based three-dimensional (3D) printing require separate matrix and ink materials, leading to complex processes and poor mechanical stability that limit precise additive manufacturing. This study aimed to develop temperature-dependent, extrusion-based 3D printing MOFs capable of balancing flowability and shape retention. Hydroxypropyl methylcellulose (HPMC), agar, and gelatin were compared as thermoresponsive hydrocolloids to identify optimal compositions exhibiting controllable viscoelastic transitions. Rheological analysis revealed that the HPMC–agar blend provided the most stable thermal behavior, where the rapid aggregation of HPMC during heating was moderated by the gradual helix reformation of agar during cooling. A 2:1 HPMC:agar ratio (24 % solids) operated around 70 °C exhibited a single crossover point and a broad transition zone, ensuring continuous extrusion and stable layer stacking. Curcumin incorporated using the amorphous solid dispersion (ASD) method retained approximately 35 % higher antioxidant activity than raw curcumin, demonstrating improved thermal and oxidative protection. Moreover, modulation of digital printing parameters—such as infill density, size, and layer number—allowed predictable control of curcumin content and disintegration onset time for personalization. The results establish a temperature-tunable and compositionally stable formulation strategy for personalized MOF fabrication applicable to functional foods and customized oral delivery systems.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108404"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted metabolomics-based investigation of amino acid effects on antimicrobial peptides production in Paenibacillus ehimensis HD 8-3 fermentation 基于非靶向代谢组学的ehimensis Paenibacillus HD 8-3发酵中氨基酸对抗菌肽产生影响的研究

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-02-02 DOI: 10.1016/j.fbio.2026.108407

Chengling Feng, Jiapei Zhang, Yunfei Si, Zhen Zhao, Yawei Ning, Zhixin Wang

{"title":"Untargeted metabolomics-based investigation of amino acid effects on antimicrobial peptides production in Paenibacillus ehimensis HD 8-3 fermentation","authors":"Chengling Feng, Jiapei Zhang, Yunfei Si, Zhen Zhao, Yawei Ning, Zhixin Wang","doi":"10.1016/j.fbio.2026.108407","DOIUrl":"10.1016/j.fbio.2026.108407","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs), as new antimicrobial products, have wide application prospects in food, medicine, and other fields. A strain of <em>Paenibacillus ehimensis</em> HD was previously identified by our research group, and the AMPs synthesized by this strain were found to be highly stable and had a broad antimicrobial spectrum. To enhance the AMPs yield, the ultraviolet-atmospheric and room temperature plasma mutagenesis (UV-ARTP) was adopted in <em>P. ehimensis</em> HD in this study. A stable mutant of <em>P. ehimensis</em> HD 8-3, with enhanced AMPs production, was obtained. Subsequently, using the resting cell culture system and untargeted metabolomics analysis, we found that the addition of L-lysine (Lys) or L-asparagine (Asn) was beneficial to the growth of <em>P. ehimensis</em> HD 8-3, while Asn also enhanced the AMPs production. The metabolites associated with the carbohydrate, glycolytic pathway, TCA cycle, pentose phosphate pathway (PPP), and amino acid metabolism were upregulated under the addition of two amino acids during the rapid growth phase of <em>P. ehimensis</em> HD 8-3, providing precursors and energy for the strain growth or AMPs production. These results indicate that the appropriate addition of exogenous Lys and Asn is an effective strategy to increase AMPs yield in <em>P. ehimensis</em> HD 8-3.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108407"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic coenzyme regulation drives high-efficiency D-tagatose biosynthesis in engineered Escherichia coli 动态辅酶调控驱动工程大肠杆菌高效d -塔格糖生物合成

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.fbio.2026.108424

Yumei Li , Yifan Tu , Zanyu Ren , Diandong Xie , Mulan Wang , Ning Li , Chen Liang , Jidong Liu

{"title":"Dynamic coenzyme regulation drives high-efficiency D-tagatose biosynthesis in engineered Escherichia coli","authors":"Yumei Li , Yifan Tu , Zanyu Ren , Diandong Xie , Mulan Wang , Ning Li , Chen Liang , Jidong Liu","doi":"10.1016/j.fbio.2026.108424","DOIUrl":"10.1016/j.fbio.2026.108424","url":null,"abstract":"<div><div>D-Tagatose is a functional rare sugar with well-established health benefits and considerable potential for use in the pharmaceutical and food sectors. Although redox-based biosynthesis serves to overcome the thermodynamic equilibrium limitations in D-tagatose production, its reaction efficiency remains a critical bottleneck. Here, we developed an engineered <em>Escherichia coli</em> catalyst for high-efficiency microbial production of D-tagatose. The biosynthetic pathway was first constructed by co-expressing galactitol-2-dehydrogenase (GDH) and xylose reductase (XR) to direct galactose-derived carbon flow toward D-tagatose. Cofactor engineering was then applied to fine-tune the balance between NADPH and NAD<sup>+</sup>. This adjusted the initial NAD<sup>+</sup>/NADH and NADP<sup>+</sup>/NADPH ratios from 0.67 to 3.42 to 0.77 and 0.88, respectively, improving the redox balance and overall performance of the pathway. Systematic metabolic tuning resulted in a 7.23-fold boost in D-tagatose generation, with the final titer reaching 34.92 g/L (yield: 0.87 g/g D-galactose, space-time yield: 0.55 g/L⋅h) under optimized whole-cell catalytic conditions. We demonstrate that the targeted modulation of NADPH–NAD<sup>+</sup> homeostasis offers a generalizable strategy for enhancing redox-dependent biotransformations. This approach provides a sustainable alternative for rare sugar biosynthesis.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108424"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retronasal persistence of fruity esters modulated by palmitic acid and ethyl palmitate: A dynamic dual-monitoring approach 由棕榈酸和棕榈酸乙酯调节的水果酯的鼻后持久性：一种动态的双重监测方法

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.fbio.2026.108349

Xinyu Zhou , Jiaheng Lyu , Dewei Zhang , Yan Xu , Ke Tang

{"title":"Retronasal persistence of fruity esters modulated by palmitic acid and ethyl palmitate: A dynamic dual-monitoring approach","authors":"Xinyu Zhou , Jiaheng Lyu , Dewei Zhang , Yan Xu , Ke Tang","doi":"10.1016/j.fbio.2026.108349","DOIUrl":"10.1016/j.fbio.2026.108349","url":null,"abstract":"<div><div>This study quantitatively evaluated how palmitic acid (PA) and ethyl palmitate (EP) affect the retronasal persistence of fruity esters in wine. The model wine containing four esters was examined after consumption by time-intensity (TI) profiling and proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). The addition of PA led to a reduction in sensory maximum intensity (I<sub>max</sub>_S) by 11–18 %, a delay in the time to maximum intensity (T<sub>max</sub>_S) by 10–15 s, and an increase in the cumulative area under the curve (AUC_S) by 15–24 %. In contrast, EP induced minor but still significant changes. Specifically, I<sub>max</sub>_S fell by 6–10 %, T<sub>max</sub>_S was delayed by 6–9 s, and AUC_S rose by 7–15 %. Instrumental data mirrored these sensory trends: PA reduced the maximum ester release (I<sub>max</sub>_R) by 6.6–83.2 μg/L and EP by 9.5–87.4 μg/L, and the time to maximum release (T<sub>max</sub>_R) was delayed by 4–13 s with PA and 3–23 s with EP. Multivariate analysis indicates that weak non-covalent interactions likely underlie the prolonged aroma persistence. These findings provide a mechanistic basis for using trace amounts of long-chain fatty acids and their ethyl esters to fine-tune retronasal aroma persistence in wine and other alcoholic beverages.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108349"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory potential of teichoic acids from Lactiplantibacillus plantarum AR113 and their effects on host immune responses 植物乳杆菌AR113中磷壁酸的抗炎作用及其对宿主免疫应答的影响

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fbio.2026.108354

Xue Li , Guangqiang Wang , Mingshu Zhang , Qixiao Zhai , Shumao Cui , Liang Zhao , Xin Song , Yongjun Xia , Zhiqiang Xiong , Lianzhong Ai

{"title":"Anti-inflammatory potential of teichoic acids from Lactiplantibacillus plantarum AR113 and their effects on host immune responses","authors":"Xue Li , Guangqiang Wang , Mingshu Zhang , Qixiao Zhai , Shumao Cui , Liang Zhao , Xin Song , Yongjun Xia , Zhiqiang Xiong , Lianzhong Ai","doi":"10.1016/j.fbio.2026.108354","DOIUrl":"10.1016/j.fbio.2026.108354","url":null,"abstract":"<div><div>Teichoic acid (TA) derived from different Gram-positive bacteria exhibit distinct effects on host inflammatory responses. In this study, we investigated the inflammation modulation of TAs from <em>Lactiplantibacillus plantarum</em> (<em>L. plantarum</em>) AR113 by AR113 key TA-synthesizing gene knockout strains. Our findings show that the presence of TAs attenuated the anti-inflammatory activity of AR113 by diminished pro-inflammatory signaling pathway triggered by TLR2 (MyD88/MAPK axis) in macrophages. And ultimately manifested <em>in vitro</em> with AR113Δ<em>ltas</em> stimulated cells to produce nitric oxide (NO) at 15.8 % lower, AR113Δ<em>dlt</em> at 48.5 % lower, and AR113ΔtagO at 51.2 % lower levels compared to AR113 (<em>p</em> < 0.05). In addition, whether <em>in vitro</em> or <em>in vivo</em>, AR113Δ<em>tagO</em> effectively could reduce pro-inflammatory cytokine production, while AR113Δ<em>ltas</em> and AR113Δ<em>dlt</em> could promoted the production of anti-inflammatory cytokine than AR113. Notably, D-Ala branch chain appears to be a key factor in the inflammatory response to TAs produced by AR113, since only the cytotoxic effect of AR113Δ<em>dlt</em> on macrophages was significantly reduced compare to AR113 (<em>p</em> < 0.01); and that AR113Δ<em>dlt</em> demonstrated the most effective relief of colitis symptoms, reducing the disease activity index (DAI) better than the AR113 intervention group (4.45 ± 1.73 vs 5.30 ± 1.85). However, further studies on the intestinal barrier in mice shown that TAs produced by AR113 had no significant effect on barrier-related genes, indicates that the TAs does not cause damage to the intestinal barrier of mice, they primarily target immune responses. Overall, this study identifies potential targets for enhancing the anti-inflammatory properties of probiotics.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108354"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated metabolomics and network pharmacology identify AKT1 as the central hub for Nymphaea candida flavonoids against acute lung injury 综合代谢组学和网络药理学鉴定AKT1是念珠花黄酮抗急性肺损伤的中心枢纽

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-01-28 DOI: 10.1016/j.fbio.2026.108388

Yue Nie , Zhen Ding , Huiliang Liu , Daoyuan Zhang , Bei Gao

{"title":"Integrated metabolomics and network pharmacology identify AKT1 as the central hub for Nymphaea candida flavonoids against acute lung injury","authors":"Yue Nie , Zhen Ding , Huiliang Liu , Daoyuan Zhang , Bei Gao","doi":"10.1016/j.fbio.2026.108388","DOIUrl":"10.1016/j.fbio.2026.108388","url":null,"abstract":"<div><div><em>Nymphaea candida</em> is traditionally used in Uyghur medicine for treating inflammatory diseases like acute lung injury (ALI), but its active components and mechanistic basis are poorly understood. This study employed an integrated strategy to predict the potential anti-ALI mechanisms of <em>N. candida</em> flower extract (NCE). Widely-targeted UPLC-MS/MS metabolomics combined with pharmacokinetic screening prioritized 29 flavonoids/polyphenols as putative bioactive constituents. Network pharmacology analysis of these compounds predicted the PI3K-AKT signaling pathway as a central therapeutic axis, with AKT1 identified as a key hub target. Molecular docking predicted strong binding affinities of five representative flavonoids (kaempferol, morin, kumatakenin, naringenin, and tamarixetin) to AKT1. These predictions were further assessed by 100-ns molecular dynamics simulations, which indicated the formation of stable flavonoid-AKT1 complexes. Notably, tamarixetin showed the most favorable predicted binding energy. All findings are computational predictions and require experimental validation. Collectively, this work provides a testable, multi-component hypothesis for the ethnopharmacological use of <em>N. candida</em> against ALI and highlights its flavonoids as promising candidates for further investigation in natural anti-inflammatory drug discovery.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108388"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a novel and thermostable laccase from Bacillus velezensis GLY4 and its application in the biotransformation of aflatoxins 一种新型耐热漆酶的鉴定及其在黄曲霉毒素生物转化中的应用

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-01-29 DOI: 10.1016/j.fbio.2026.108389

Xin Zhang , Binbin Ouyang , Wei Xu , Hongbin Sun , Wanmeng Mu

{"title":"Identification of a novel and thermostable laccase from Bacillus velezensis GLY4 and its application in the biotransformation of aflatoxins","authors":"Xin Zhang , Binbin Ouyang , Wei Xu , Hongbin Sun , Wanmeng Mu","doi":"10.1016/j.fbio.2026.108389","DOIUrl":"10.1016/j.fbio.2026.108389","url":null,"abstract":"<div><div>Recently, enzyme-based transformation of aflatoxins (AFs) has drawn great attention. The microbial laccases are most intensively studied. This study identified a thermostable laccase (designated LacGLY4) from <em>Bacillus velezensis</em> GLY4. The recombinant enzyme could be smoothly expressed in <em>Escherichia coli</em> and purified by Ni<sup>2+</sup>-affinity chromatography. The LacGLY4 displayed the optimal pH and temperature at pH 8.0 and 85 °C, and maintained a relatively high activity at 75–95 °C against AFB<sub>1</sub>. At the optimized condition, over 95 % of AFB<sub>1</sub> (10 μg/mL) would be transformed by 20 μg/mL of LacGLY4 after 30 min. The half-life (<em>t</em><sub>1/2</sub>) of LacGLY4 at 40, 50 and 60 °C was calculated to be 77, 63 and 53 min, respectively. LacGLY4 could extensively transform a variety of AFB<sub>1</sub> analogues that showed the specific activity of 0.132, 0.077, 0.071, and 0.107 U/mg for AFB<sub>2</sub>, AFM<sub>1</sub>, AFG<sub>1</sub> and AFG<sub>2</sub>, respectively. In addition, the LacGLY4 exhibited about 57 % relative activity towards ZEN, but only showed less than 10 % of activity against DON and PAT compared to that of AFB<sub>1</sub>. A significant transformation product from AFB<sub>1</sub> by LacGLY4 was identified as C<sub>24</sub>H<sub>30</sub>O<sub>6</sub> by liquid chromatography–tandem mass spectrometry. The generated product is speculated to be formed by a complex addition reduction rather than a simple hydrolase reaction from AFB<sub>1</sub>. This bacterial laccase combines good thermostability and efficient transformation, providing a novel enzymatic tool for the biocontrol of AFB<sub>1</sub> contamination such as in the oil refinement degumming step in which the temperature is around 80 °C and the processing time is about 30 min.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108389"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heyndrickxia coagulans JF1 with potent ethanol-degrading capacity: In vitro screening and functional characterization 具有有效乙醇降解能力的海因德里克夏凝血JF1：体外筛选和功能表征

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-01-22 DOI: 10.1016/j.fbio.2026.108352

Feiyu Yang , Xiangfei Li , Jing Sun , Xinyi Pang , Yonghong Hu , Chi Chen , Yingjian Lu , Fengxia Lv

{"title":"Heyndrickxia coagulans JF1 with potent ethanol-degrading capacity: In vitro screening and functional characterization","authors":"Feiyu Yang , Xiangfei Li , Jing Sun , Xinyi Pang , Yonghong Hu , Chi Chen , Yingjian Lu , Fengxia Lv","doi":"10.1016/j.fbio.2026.108352","DOIUrl":"10.1016/j.fbio.2026.108352","url":null,"abstract":"<div><div>Excessive acute alcohol intake can overload hepatic ethanol metabolic pathways, inducing oxidative stress responses, lipid metabolism disorders, and hepatocyte apoptotic cascades. Current clinical interventions are mostly symptomatic supportive care, and there remains a lack of effective strategies to directly convert ethanol into non-toxic metabolites in the digestive tract. In this study, <em>Heyndrickxia (H.) coagulans</em> JF1 was isolated from samples of chicken intestinal contents, and its ethanol-degrading capacity and probiotic functional characteristics were systematically evaluated. Notably, <em>H. coagulans</em> JF1 exhibited excellent ethanol-degrading capacity, ethanol tolerance, and gastrointestinal environment tolerance. This strain showed good adhesion to Caco-2 cells, along with remarkable hydrophobicity, auto-aggregation, and co-aggregation abilities. Antioxidant activity assays demonstrated that JF1 possesses strong scavenging capacity against various free radicals, with cell lysates displaying higher antioxidant levels. Antibacterial activity tests indicated that JF1 can inhibit five species of foodborne pathogens, is susceptible to ten antibiotics, and exhibits no hemolytic activity. In cellular experiments, intervention with JF1 regulated the expression of genes related to oxidative stress, lipid metabolism, immunity, autophagy, and apoptosis in ethanol-induced HepG2 cells. Whole-genome sequencing of JF1 revealed its genetic composition and relevant metabolic pathways, providing a genetic basis for its functional characteristics. Through multi-dimensional phenotypic analysis and genomic characterization, this study offers important theoretical and experimental support for the development of <em>H. coagulans</em> JF1 as a functional probiotic that promotes ethanol metabolism in the body.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108352"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the delivery mechanism of gallic acid grafted chitosan-ovalbumin nanoparticles for quercetin: Structural characterisation, stability, and intermolecular interactions 没食子酸接枝壳聚糖-卵清蛋白纳米颗粒递送槲皮素机制的研究：结构表征、稳定性和分子间相互作用

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.fbio.2026.108373

Jianyu Huang , Tao Huang , Qianqian Zhang , Ziying Ruan , Dengmi Wang , Peichao Zhang , Zhizhi Yang , Hui Xu , Jinjun Li , Jicheng Chen

{"title":"Study on the delivery mechanism of gallic acid grafted chitosan-ovalbumin nanoparticles for quercetin: Structural characterisation, stability, and intermolecular interactions","authors":"Jianyu Huang , Tao Huang , Qianqian Zhang , Ziying Ruan , Dengmi Wang , Peichao Zhang , Zhizhi Yang , Hui Xu , Jinjun Li , Jicheng Chen","doi":"10.1016/j.fbio.2026.108373","DOIUrl":"10.1016/j.fbio.2026.108373","url":null,"abstract":"<div><div>Quercetin (Que) has substantial health benefits but is limited by poor solubility, low bioavailability, and instability. This study developed composite delivery system (GOQ) via layer-by-layer assembly of gallic acid grafted chitosan (GCS) and ovalbumin (OVA) for efficient Que encapsulation. Characterization showed: the shell formed through electrostatic interactions between GCS amino groups and OVA carboxyl groups; Que embedded in OVA's hydrophobic cavity via hydrophobic interactions, inducing OVA fluorescence quenching, converting Que to an amorphous state, yielding uniform nanoparticles. GOQ exhibited excellent stability across 60–90 °C, pH 3–8, and 10–30 mM ionic concentrations. In simulated digestion, it resisted gastric degradation, disintegrated controllably in the intestine, fitted zero-order, first-order, and Peppas models (sustained release), and enhanced Que's solubility and cellular uptake. Molecular analyses revealed GCS provided more hydrogen bonds, compacting protein structure, reducing residue flexibility and surface exposure, and boosting stability. This strategy overcomes Que's limitations, supporting its use in functional foods and drug delivery.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"77 ","pages":"Article 108373"},"PeriodicalIF":5.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147399295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0