Food Bioscience最新文献

{"title":"Rheological, hygroscopic, and bioactive properties of prebiotic dextran from Limosilactobacillus fermentum CFW6: Optimization and application as a texture enhancer in soy and ragi milk","authors":"Megha Mete ,&nbsp;Amiya Ojha ,&nbsp;Hriddhi Bhattacharjee ,&nbsp;Deeplina Das","doi":"10.1016/j.fbio.2025.106858","DOIUrl":"10.1016/j.fbio.2025.106858","url":null,"abstract":"<div><div>The growing demand for natural bioactive compounds has intensified interest in microbial exopolysaccharides (EPS), particularly those produced by lactic acid bacteria, owing to their diverse functionality and potential industrial applications. In this study, a new strain <em>Limosilactobacillus fermentum</em> CFW6 (GenBank Accession No: MF156856), isolated from pineapple pomace, was evaluated for its EPS-producing ability. EPS yield increased from 2.84 mg/mL under unoptimized conditions to 6.84 mg/mL following sequential optimization using Plackett–Burman design and response surface methodology, a nearly threefold enhancement, surpassing previously reported yields from similar strains. The purified EPS was structurally characterized using Fourier transform infrared spectroscopy (FT-IR), ¹H NMR, and ¹³C NMR, confirming it as a dextran polymer with an α-(1 → 6) glycosidic linkage. Additionally, Field emission scanning electron microscopy (FESEM) and Energy dispersive X-ray spectroscopy (EDX) analyses revealed a compact, porous, sheet-like structure, predominantly composed of carbon (43.62%) and oxygen (33.53 %). Rheological profiling indicated pseudo-plastic fluid behavior, ideal for dairy processing applications, as the apparent viscosity decreased with increasing shear rate. Bioactivity assessments revealed that EPS CFW6 exhibited significant emulsification activity (58.49 %), flocculation activity (66.23%), water retention capacity (169.66%), solubility (86.25%), hygroscopicity (17.38%), antioxidant activity (60.29%), and low digestibility (6.84 %) in gastrointestinal fluid, supporting probiotic growth and underscoring its potential as a prebiotic food additive. Furthermore, <em>in situ</em> EPS production during soy and ragi milk fermentation significantly improved viscosity (∼58 %), consistency index (∼87 %), titratable acidity (∼85°T), and water retention capacity (P &lt; 0.05), offering a promising strategy to enhance the texture and quality of plant-based dairy alternatives.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106858"},"PeriodicalIF":4.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotics ameliorate DSS-induced colitis in mice by regulating gut microbiota, promoting tryptophan metabolism, and activating AhR/ IL-22 signaling pathway","authors":"Mengzhen Han,&nbsp;Mengxin Hou,&nbsp;Yuanye Liu,&nbsp;Hongcai Li,&nbsp;Zhenpeng Gao","doi":"10.1016/j.fbio.2025.106828","DOIUrl":"10.1016/j.fbio.2025.106828","url":null,"abstract":"<div><div>Inactivated probiotics, due to their high safety, effectiveness, stability, and resilience, are increasingly used for treating various diseases. To assess the efficacy of inactivated <em>Bifidobacterium bifidum</em> M1-3 in alleviating dextran sulfate sodium salt (DSS) -induced colitis, we compared the effects of live probiotics and inactivated probiotics at different concentrations on colitis in male C57BL/6J mice. Additionally, we explored potential mechanisms by analyzing changes in gut microbiota composition, tryptophan metabolites, and the AhR/IL-22 signaling pathway. Our study demonstrated that administering high doses (2 × 10⁹ CFU/mL) of heat-inactivated probiotics by gavage for 14 days effectively alleviated DSS-induced colitis in mice, with effects comparable to those of live probiotics. This effect was attributed to the modulation of gut microbiota, with an increase in beneficial bacteria like <em>Muribaculaceae</em>, <em>Alistipes</em>, and <em>Ruminococcaceae</em>, and a reduction in harmful microbes such as <em>Escherichia/Shigella</em> spp. Additionally, the inactivated probiotics enhanced tryptophan metabolism, leading to elevated levels of metabolites including indole-3-lactic acid, indole-3-propionic acid, indole acrylic acid, and indole, which subsequently activated the AhR/IL-22 signaling pathway. Our results confirmed the effectiveness of heat-inactivated <em>Bifidobacterium bifidum</em> M1-3 in alleviating colitis, demonstrating a dose-dependent response. The soluble capsular polysaccharides in the bacterial lysate supernatant are the potentially effective components of <em>Bifidobacterium bifidum</em> M1-3. This finding provides a theoretical basis for developing novel probiotic products containing heat- inactivated <em>Bifidobacterium bifidum</em> M1-3.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106828"},"PeriodicalIF":4.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6-Gingerol and 6-shogaol attenuate chemotherapy-induced nausea and vomiting via ameliorating mitochondrial dysfunction and pyroptosis through JNK/MFF signaling pathway","authors":"Yarong Zhai,&nbsp;Lei Feng,&nbsp;Ziyao Mo,&nbsp;Binbin Ye,&nbsp;Wan Liang,&nbsp;Ke Nie","doi":"10.1016/j.fbio.2025.106808","DOIUrl":"10.1016/j.fbio.2025.106808","url":null,"abstract":"<div><div>6-Gingerol and 6-shogaol, the main active compounds in ginger, have been proven to have antiemetic effect, and can be employed to alleviated chemotherapy-induced nausea and vomiting (CINV), but the underlying mechanisms remain unclear. We aimed to investigate whether 6-gingerol and 6-shogaol attenuate CINV through ameliorating mitochondrial dysfunction and pyroptosis-induced gastrointestinal inflammation injuries. Cisplatin-induced pica rat model was established to investigate the antiemetic effects of 6-gingerol (100 mg/kg) and 6-shogaol (100 mg/kg) against CINV. Cisplatin-stimulated IEC-6 cell inflammation injury model was established to further explore the protective mechanisms. The antiemetic efficacy was observed by kaolin intake (a pica response analogous to nausea and vomiting). Here, in vivo, 6-gingerol and 6-shogaol significantly reduced the kaolin intake, alleviated gastrointestinal injury, and decreased the levels of inflammatory cytokines (IL-1β, TNF-α) in pica rats. Moreover, 6-gingerol and 6-shogaol reduced mitochondrial damage, modulated disturbed mitochondrial dynamics, and inhibited GSDME-mediated pyroptosis. In vitro, 6-gingerol and 6-shogaol significantly restored mitochondrial membrane potential and morphology. Mechanistically, we found that 6-gingerol and 6-shogaol blocked the JNK/MFF signaling pathway, which attenuated mitochondrial dysfunction and subsequently prevented GSDME-mediated pyroptosis. Collectively, this study suggested that 6-gingerol and 6-shogaol have antiemetic effects against CINV, which are associated with inhibiting mitochondrial dysfunction and GSDME-mediated pyroptosis via JNK/MFF pathway.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106808"},"PeriodicalIF":4.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of quorum sensing effector ComA in regulating biofilm formation and surfactin production in Bacillus subtilis ASAG 010","authors":"Ru Jia ,&nbsp;Yu Wang ,&nbsp;Huachao Wang ,&nbsp;Xiao Ji ,&nbsp;Faizan Ahmed Sadiq ,&nbsp;Xu Wang ,&nbsp;Guohua Zhang","doi":"10.1016/j.fbio.2025.106842","DOIUrl":"10.1016/j.fbio.2025.106842","url":null,"abstract":"<div><div><em>Bacillus</em> species are valuable agents in biocontrol and sustainable agriculture. This study focuses on the properties of <em>Bacillus subtilis</em> ASAG 010, a strain isolated from wheat-associated soil, which exhibited potent antifungal activity against multiple plant pathogens, including <em>Fusarium graminearum</em>. The strain effectively controlled <em>Fusarium</em> head blight in wheat and demonstrated the ability to degrade the mycotoxin zearalenone (ZEN). High-performance liquid chromatography (HPLC) and MALDI-TOF-MS analyses, coupled with genetic verification, identified surfactin as the primary antifungal compound produced by <em>B. subtilis</em> ASAG 010. Analysis of the static fermentation process revealed that a mature biofilm structure significantly enhanced surfactin synthesis in <em>B. subtilis</em> ASAG 010. To investigate the interplay between quorum sensing (QS), biofilm formation, and surfactin production, a <em>ComA</em>-deficient mutant was constructed. The mutant exhibited reduced extracellular polysaccharides, proteins, and γ-PGA – key biofilm matrix components – and lower expression of <em>EpsA</em> and <em>BlsA</em>. HPLC analysis showed a significantly reduced in surfactin production, and DNA pull-down assays confirmed that <em>ComA</em> directly binds upstream of the <em>srfA</em> gene, which encodes surfactin biosynthesis. These findings establish ComA as a key regulator of biofilm integrity and surfactin production, providing insights into the biocontrol potential of <em>B. subtilis</em> ASAG 010 for food safety and agricultural sustainability.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106842"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive UPLC-MS/MS-based metabolic profiling, antioxidant potential, enzyme inhibition, and molecular docking studies of Ficus carica L. leaves: Potential for functional food applications","authors":"Lale Duysak ,&nbsp;Adem Ertürk ,&nbsp;Merve Becit-Kızılkaya ,&nbsp;İlhami Gülçin","doi":"10.1016/j.fbio.2025.106835","DOIUrl":"10.1016/j.fbio.2025.106835","url":null,"abstract":"<div><div>Fig (<em>Ficus carica</em> L.) is a fruit tree consumed worldwide as a functional food. While its fruit is well-studied, the phytochemicals and biological activities of its leaves have not been reported in detail. This study comprehensively investigated phytochemical composition and biological activities of methanol extract from <em>Ficus carica</em> L. leaves from Nazilli, Türkiye. Phytochemical analysis was performed using UPLC-MS/MS. Antioxidant capacity was evaluated using electron transfer-based analyses (Fe3+ reduction, CUPRAC, FRAP) and radical scavenging methods (DPPH, ABTS, DMPD). Enzyme inhibition effects were examined on carbonic anhydrases (CA I-II), acetylcholinesterase (AChE), and α-glycosidase (α-Gly). High total phenolics (86.111 μg GAE/mg) and flavonoids (104.086 μg QE/mg) were detected. UPLC-MS/MS identified 17 bioactive compounds, with chlorogenic acid (17069.52 ng/mL) as dominant. The extract showed notable antioxidant activity, performing best in CUPRAC (exceeding Trolox) and ABTS assays. It significantly inhibited all tested enzymes with IC₅₀ values of 0.024 μg/mL for CA I, 0.025 μg/mL for CA II (approximately 4-fold higher than acetazolamide), 0.002 μg/mL for AChE (half as potent as tacrine: 0.001 μg/mL), and 0.009 μg/mL for α-Gly (1.7-fold more potent than acarbose: 0.015 μg/mL). Molecular docking confirmed favorable binding energies (all below −7.00 kcal/mol) between chlorogenic acid and target enzymes. This study represents the first comprehensive metabolic profiling of fig leaves from Nazilli region and reveals their potential as natural ingredients for nutraceutical products owing to their rich phenolic content, notable antioxidant capacity, and potent enzyme inhibitory properties relevant to dietary management of various health conditions.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106835"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-biofortifying crops with micronutrients and beneficial elements: Toward improved global nutrition","authors":"Hipólito Hernández-Hernández ,&nbsp;Antonio Juárez-Maldonado","doi":"10.1016/j.fbio.2025.106848","DOIUrl":"10.1016/j.fbio.2025.106848","url":null,"abstract":"<div><h3>Background</h3><div>Micronutrient deficiency, or “hidden hunger”, affects over 3 billion people globally, compromising health and food security, especially in developing countries. Conventional fertilizers used in agronomic biofortification often show limited nutrient use efficiency and environmental sustainability.</div></div><div><h3>Methods</h3><div>This review analyzed recent studies on the application of nano-fertilizers enriched with Zn, Fe, Se, Cu, Co, Ni, and Si for the biofortification of edible crop parts.</div></div><div><h3>Main findings</h3><div>Nano-biofortification significantly increases nutrient concentrations in grains, fruits, and leaves. For example, foliar application of ZnO nanoparticles increased grain zinc by 82 % (54.6 mg kg⁻¹) in corn compared to controls. Fe₂O₃ nanoparticles improved iron content in wheat grains by up to 72 % (80 mg kg⁻¹). Selenium nanoparticles enhanced Se bio-accessibility in soybean sprouts, and CoFe₂O₄ nanoparticles improved both bio-accessibility and safety in wheat.</div></div><div><h3>Conclusions</h3><div>Nano-biofortification emerges as a promising strategy to enhance crop nutritional quality, contributing to global food and nutrition security. However, challenges such as production costs, regulatory gaps, and environmental risks must be addressed for safe and scalable implementation.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106848"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α-Linolenic acid prevents experimental osteoporosis by inhibiting Ca2+/CaMKIV/p-CREB pathway and typical RANK signaling","authors":"Jin Qian ,&nbsp;Jinge Li ,&nbsp;Jinxiang Chen ,&nbsp;Zhijie Yao ,&nbsp;Shuxiao Liang ,&nbsp;Qian Zhou ,&nbsp;Mingfu Wang ,&nbsp;Haitao Li","doi":"10.1016/j.fbio.2025.106807","DOIUrl":"10.1016/j.fbio.2025.106807","url":null,"abstract":"<div><div>Epidemiological studies suggest an association between edible oil intake and the development of osteoporosis. However, whether such a relationship is causal remains to be validated. In this study, we discovered that the administration of diverse edible oils elicited distinct pathological responses within an ovariectomy (OVX)-induced osteoporosis model. Data showed that linseed oil exhibited a great improvement, significantly enhancing biochemical indices related to bone metabolism, biomechanical properties, and microstructure of bone tissue. Notably, diets with different oils altered the fatty acids composition in femur, thus remodeling the homeostasis of bone metabolism. The α-linolenic acid (ALA) content in femur of mice in linseed oil group was increased by 14.71 ± 0.34-fold, representing the most pronounced change among all the groups. Mechanistically, ALA inhibited the proliferation and formation of osteoclast, as well as osteoclastogenesis-related gene expression <em>in vitro</em>. Furthermore, ALA mitigated receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and bone loss by suppressing the Ca²⁺/CaMKIV/p-CREB pathway and typical RANK signaling. Overall, these findings imply that dietary supplementation with linseed oil could be an effective strategy for OVX-induced osteoporosis, with ALA being the bioactive component conferring the anti-osteoporotic properties of linseed oil.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106807"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining the possibility of producing natural microbicides and antioxidant agents for food and cosmetic uses from the essential oils of Laurus nobilis (laurel), Syzygium aromaticum (clove), and Cinnamomum verum (cinnamon)","authors":"Metin Oktay Beyaz ,&nbsp;Ahmet E. Yetiman ,&nbsp;Mahmut Doğan ,&nbsp;Mehmet Horzum","doi":"10.1016/j.fbio.2025.106840","DOIUrl":"10.1016/j.fbio.2025.106840","url":null,"abstract":"<div><div>The objectives of this study were to evaluate the chemical composition, antioxidant and microbicide properties, and cytotoxic effects of essential oils acquired from Laurel (<em>Laurus nobilis</em>), Clove (<em>Syzygium aromaticum</em>), and Cinnamon (<em>Cinnamomum verum</em>) by hydrodistillation. GC-MS analysis identified 1,8-cineole (48.50 %), eugenol (49.49 %), and cinnamaldehyde (65.34 %) as the major constituents of laurel, clove, and cinnamon essential oils, respectively. Antioxidant capacity, assessed by ABTS⁺ and DPPH assays, revealed that cinnamon essential oil exhibited the highest inhibition percentage. Antimicrobial testing showed cinnamon oil was most effective against tested pathogens, with an average inhibition zone of 28 mm. On the other hand, cytotoxicity was evaluated using the MTT assay on L-929 fibroblast cells, and no harmful effects were observed for clove and cinnamon oils. The protective effect of the oils has been tested against total mesophilic aerobic bacteria (TMAB) and yeast-mold (YM) count in a preservative-free tomato paste model food environment with a 25-day storage period. At the end of 25 days, the control group had 3 × 10² cfu/g excess colony formation, but the samples treated with 1.5 % concentration had 1.5 × 10¹ cfu/g mean count. Furthermore, a hand sanitizer formulation containing these oils suppressed microorganisms by 85 %, whereas the addition of isopropyl alcohol (25 %) increased the activity to 98 %. These findings demonstrate that clove and cinnamon essential oils possess strong inhibitory effects against microbial growth and oxidation, supporting their potential as natural alternatives to synthetic preservatives and disinfectants.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106840"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of MLM-type structured lipids from avocado oil catalyzed by immobilized lipase on corn cob powder: Assessment of antiproliferative potential on Hep-G2 cell line","authors":"Maryli Jessenia Bohorquez-Peña ,&nbsp;Brandon Vargas-Suaza ,&nbsp;Kevin Esteban Garzón-Alonso ,&nbsp;Julio Cesar Rendón-Londoño ,&nbsp;Yuly Nataly Franco-Tobón ,&nbsp;Claudia Lorena Gómez-Herrera ,&nbsp;Mónica Mesa ,&nbsp;Julián Paul Martínez-Galán","doi":"10.1016/j.fbio.2025.106824","DOIUrl":"10.1016/j.fbio.2025.106824","url":null,"abstract":"<div><div>The demand for natural and healthy products has driven research into new value-added products derived from avocado (<em>Persea Americana</em> var. <em>Hass</em>) oil. For example, the exploration of the production of structured lipids (SL), which are modified triacylglycerides with enhanced nutritional and functional properties. Enzymatic engineering of structured lipids using immobilized lipases allows for greater economy and stability under reaction conditions. In this study, medium- and long-chain structured lipids were produced from avocado oil caprylic acid by enzymatic acidolysis. Lipase from <em>Thermomyces lanuginosus</em> was immobilized on corn cob powder. Efficient incorporation of caprylic acid (C8:0) into avocado oil was achieved with a maximum of 31.11 % at <em>sn</em>-1 or <em>sn</em>-3 positions. However, incorporation did not occur simultaneously into triacylglycerides, and lipase reusability of up to 4 cycles. In the hepatocarcinoma cell line Hep-G2, the structured and hydrolyzed lipids inhibited cell viability, suggesting their potential for future studies related to the maintenance of liver health.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106824"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient conversion of ᴅ-fructose to ᴅ-mannose enabled by coupled reactions of mannose isomerase and sugar kinase with co-factor regeneration","authors":"Di Ma,&nbsp;Xiaocong Wu,&nbsp;Haodong Wu,&nbsp;Guofeng Gu,&nbsp;Xianwei Liu","doi":"10.1016/j.fbio.2025.106841","DOIUrl":"10.1016/j.fbio.2025.106841","url":null,"abstract":"<div><div>ᴅ-Mannose is a functional monosaccharide that is difficult to prepare on a large scale via chemical or enzymatic synthetic routes due to low conversion rates and complex downstream isolation. In this study, a two-step enzymatic route was established to achieve highly efficient conversion of ᴅ-fructose to ᴅ-mannose without the need for a tedious isomer separation process. Firstly, a mannose isomerase from <em>Xanthomonas phaseoli</em> was coupled with a sugar 1-kinase from <em>Bifidobacterium infantis</em> and a polyphosphate kinase to synthesize mannose 1-phosphate (Man-1-P) from ᴅ-fructose and polyphosphate (PolyP), using adenosine 5′-triphosphate or adenosine 5′-monophosphate (AMP) as the regenerating co-factor. The reaction was optimized to achieve 99 % conversion of ᴅ-fructose at substrate concentrations of 300 mmol/L for ᴅ-fructose, with only 0.5 mmol/L AMP (1/600 equiv.) as the co-factor. The solution from the first-step reaction, containing Man-1-P, was simply clarified by centrifugation and then precipitated with calcium salt. Subsequently, Man-1-P was hydrolyzed by a phosphatase to yield ᴅ-mannose, which was purified by desalting with activated carbon adsorption. As a demonstration of this strategy, 11.1 g of ᴅ-mannose was produced with an overall yield of 85.6 %. This synthesis strategy is expected to be applicable for large-scale preparation.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"69 ","pages":"Article 106841"},"PeriodicalIF":4.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}