Food BiosciencePub Date : 2025-07-11DOI: 10.1016/j.fbio.2025.107230
Youning Zou , Yiting Fan , Jiahao Ren , Yifang Cao , Bowen Qi , Xuying Luo , Fengsong Liu , Xinglong Xiao
{"title":"Recent advances in antibacterial food preservation using soy protein isolate-stabilized essential oil nanoemulsions","authors":"Youning Zou , Yiting Fan , Jiahao Ren , Yifang Cao , Bowen Qi , Xuying Luo , Fengsong Liu , Xinglong Xiao","doi":"10.1016/j.fbio.2025.107230","DOIUrl":"10.1016/j.fbio.2025.107230","url":null,"abstract":"<div><div>With increasing demand for safe, natural, and sustainable food preservation, soy protein isolate (SPI)-based nanoemulsions have emerged as promising carriers for bioactive compounds such as essential oils (EOs). Several techniques, including high-pressure homogenization, microfluidization, ultrasonication, spontaneous emulsification, phase inversion temperature (PIT), and phase inversion composition (PIC), have been employed to fabricate SPI-based EO nanoemulsions with desirable droplets (50–200 nm) and improved physicochemical stability (up to 30–60 days). Mechanistic insights into stabilization strategies, including SPI secondary structure transitions (e.g., β-sheet enrichment), covalent/non-covalent SPI–polyphenol interactions, and Pickering-like interfacial structuring, are critically examined. This review systematically categorized SPI-based emulsifiers, elucidated the functional characteristics of SPI-stabilized EO nanoemulsions, and discussed the key factors influencing their stability, biofunctionality, and applications in meat, fruits, and dairy matrices. Moreover, this review uniquely connects formulation parameters to food-specific outcomes, and discusses challenges such as EO volatility, sensory impact, and safety evaluation. Finally, regulatory considerations and future perspectives on improving delivery efficacy, stability, and nutritional compatibility are addressed. This comprehensive synthesis offers a practical framework for the design and food industry application of SPI-based nanoemulsions.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107230"},"PeriodicalIF":4.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-07-10DOI: 10.1016/j.fbio.2025.107223
Ranjina Ranjith , Basavaraj S. Nainegali , Subbalaxmi Selvaraj
{"title":"Nurturing with science: Alternative solution for human milk oligosaccharides through bioproduction techniques and their emerging applications in infant nutrition","authors":"Ranjina Ranjith , Basavaraj S. Nainegali , Subbalaxmi Selvaraj","doi":"10.1016/j.fbio.2025.107223","DOIUrl":"10.1016/j.fbio.2025.107223","url":null,"abstract":"<div><div>Human Milk Oligosaccharides (HMOs) are bioactive carbohydrates found in human milk that play a crucial role in infant growth and health. However, due to several factors, such as maternal health conditions, lactation insufficiency, and socio-economic barriers, breastfeeding is not always feasible. This has led to the need for the synthesis of alternative HMOs to bridge the nutritional gap for non-breastfed infants. First, this review explains various physiological roles of HMOs in the overall growth and development of infants, followed by extraction of alternative oligosaccharides from multiple natural sources, including the milk of animals such as cows, goats, and camels, as well as from plant and marine sources. However, this method is limited by low yield, high purification cost, and significant compositional variability. Thus, advanced biotechnological processes for industrial-level production, such as microbial fermentation and enzymatic and chemical synthesis, including purification and characterization techniques, are discussed in this review. This review also evaluates each production method, emphasizing the industrial feasibility, scalability, and cost-effectiveness. The advantages and challenges of each large-scale production method are analyzed, followed by a discussion of emerging advancements in biotechnology and methodologies for improved HMO production. The review also highlights the clinical trials conducted in infants to assess the safety and efficiency of synthesized HMOs, as well as about the regulatory approvals that govern their addition in infant formulas and other supplementary products. Finally, various potential applications of alternative HMOs, like infant nutrition, pharmaceuticals, functional food, and emerging applications, are highlighted.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107223"},"PeriodicalIF":4.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-07-09DOI: 10.1016/j.fbio.2025.107221
Dongsheng Bian , Songyue Su , Xinyi Cheng , Yongmei Shi , Xingguo Wang , Yuanyuan Yan
{"title":"n-3 Polyunsaturated fatty acids improve sarcopenia by mitochondrial quality control","authors":"Dongsheng Bian , Songyue Su , Xinyi Cheng , Yongmei Shi , Xingguo Wang , Yuanyuan Yan","doi":"10.1016/j.fbio.2025.107221","DOIUrl":"10.1016/j.fbio.2025.107221","url":null,"abstract":"<div><div>Accelerated global population aging has established sarcopenia as a critical public health burden, directly compromising health and independence in older adults. Converging evidence implicates mitochondrial dysfunction as pathogenic in sarcopenia, manifested through defective energy production, heightened oxidative stress, and compromised mitochondrial quality control (MQC). n-3 Polyunsaturated fatty acids (n-3 PUFAs), specifically eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), demonstrate significant potential to ameliorate mitochondrial impairments. Their mechanisms encompass: enhanced oxidative phosphorylation (OXPHOS) efficiency, activation of the NRF2-mediated antioxidant response, restoration of mitochondrial fission/fusion dynamics, and facilitation of PINK1/Parkin-dependent mitophagy. Critically, n-3 PUFAs activate the AMPK/SIRT1/PGC-1α axis to stimulate mitochondrial biogenesis, thereby augmenting mitochondrial content and supporting muscle regeneration. Notably, preclinical studies indicate superior efficacy of EPA over DHA in modulating MQC and preserving muscle function. This review synthesizes current mechanistic insights into n-3 PUFAs-mediated regulation of mitochondrial quality control and evaluates their therapeutic promise for attenuating age-related sarcopenia.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107221"},"PeriodicalIF":4.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-07-08DOI: 10.1016/j.fbio.2025.107218
Mengyue Liu , Giovanna Marrazza , Yemin Guo , Xia Sun , Shicai Xu
{"title":"Current advances in aptamer-functionalized DNA nanostructures for pathogenic bacteria detection in food","authors":"Mengyue Liu , Giovanna Marrazza , Yemin Guo , Xia Sun , Shicai Xu","doi":"10.1016/j.fbio.2025.107218","DOIUrl":"10.1016/j.fbio.2025.107218","url":null,"abstract":"<div><div>Pathogenic bacteria are widely present in food and the environment, posing a serious threat to human health. The key to addressing this issue lies in the development of rapid and effective sensors for the sensitive detection of pathogenic bacteria. Aptamer-functionalized DNA nanostructures represent a cutting-edge approach in biosensing, offering high specificity and versatility for pathogenic bacteria detection. By integrating synthetic aptamers with programmable DNA nanostructures, researchers are developing innovative tools that surpass traditional methods in speed, sensitivity, and portability. This review focuses on the design, types, and applications of aptamer-functionalized DNA nanostructures in the field of pathogenic bacteria detection. We provide a detailed analysis of the assembly methods, characteristics, and functionalities of these nanostructures. Additionally, this work summarizes the applications of aptamer-functionalized DNA nano-sensors based on various sensing signals. Finally, we discuss current challenges and future prospects to inspire advancements in this dynamic field.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107218"},"PeriodicalIF":4.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-07-07DOI: 10.1016/j.fbio.2025.107202
Nelson Kei , Susana Lauw , Peter Chi Keung Cheung
{"title":"Analytical strategies for gut microbiome studies of prebiotic β-glucan in metabolic dysfunction-associated steatotic liver disease: A mini-review","authors":"Nelson Kei , Susana Lauw , Peter Chi Keung Cheung","doi":"10.1016/j.fbio.2025.107202","DOIUrl":"10.1016/j.fbio.2025.107202","url":null,"abstract":"<div><div>High-throughput sequencing techniques have become essential tools for understanding how prebiotic β-glucan modulates the gut microbiome to alleviate metabolic dysfunction-associated steatotic liver disease (MASLD). However, studies in this area vary considerably in their choice of sequencing methods (16S rRNA gene sequencing or shotgun metagenomic sequencing), sampling sites, and analyses of microbial diversity (α and β), differential abundance, functions, correlation, and underlying mechanisms. This mini-review summarizes current approaches from β-glucan-related microbiome studies in the context of MASLD and highlights the need for standardized research practices. While this review focuses on β-glucan, the proposed recommendations may also guide future research on other emerging prebiotics aimed at preventing or treating different diseases.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107202"},"PeriodicalIF":4.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytic acid: biosynthesis, functional attributes and conventional vis-a-vis modern approaches for reduction","authors":"Pragya , Pradeep Kumar , Davender Singh , Santosh Kumar Tiwari , Hichem Chouayekh , Bijender Singh","doi":"10.1016/j.fbio.2025.107204","DOIUrl":"10.1016/j.fbio.2025.107204","url":null,"abstract":"<div><div>Phytic acid is plant-based organic phosphorus stored during maturation. It serves a dual purpose in plant tissues by acting as storage for phosphorus and regulates various cellular processes. From a nutritional perspective, chelating ability of phytic acid is considered as a potential health drawback, while in other ways as a most valuable trait. Phytic acid accumulates in seeds during their developmental stages and is synthesized through two distinct pathways i.e. one is independent of lipids and the other is dependent on lipids. Beginning with a simple precipitation method involving the formation of insoluble ferric phytate in an acidic solution, the methods for quantifying phytic acid have evolved over time to encompass various instrumental approaches, including colorimetry, high-performance ionic chromatography, and high-performance liquid chromatography. Enzymatic reduction using phytases plays a crucial role in converting phytic acid into different inositol phosphates and inorganic phosphorus. Decreasing the phytic acid content in cereal grains is a desirable objective for development of genetically improved crops, which are used in food and feed applications. Phytic acid is not a foe as it has been shown in literature studies. It performs various metabolic functions as well as acts as a reservoir of nutrients for plants and animals. This review explores the advantages and disadvantages of phytic acid, its accumulation, estimation, and reduction using modern tools of genome editing, such as transcription activator-like effector nucleases (TALEN), zinc-finger nucleases (ZFN), and clustered regularly interspaced short palindromic repeats with CRISPR-associated protein 9 (CRISPR/Cas9).</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107204"},"PeriodicalIF":4.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-07-05DOI: 10.1016/j.fbio.2025.107190
Wanyu Yang, Huizi Tan, Shaoping Nie
{"title":"A Low-FODMAP diet enhances IBS symptom relief and gut microbiota homeostasis: A meta-analysis","authors":"Wanyu Yang, Huizi Tan, Shaoping Nie","doi":"10.1016/j.fbio.2025.107190","DOIUrl":"10.1016/j.fbio.2025.107190","url":null,"abstract":"<div><h3>Background</h3><div>Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder worldwide. The low-FODMAP diet (LFD), which restricts fermentable oligo-, di- and monosaccharides and polyols, has demonstrated potential efficacy in alleviating symptoms; however, current evidence remains conflicting. This study aims to integrate randomized controlled trials (RCTs) assessing LFD's impact on IBS through meta-analysis, thereby establishing a scientific foundation for clinical management.</div></div><div><h3>Methods</h3><div>We conducted a systematic literature review using databases including PubMed, Cochrane Library, Web of Science, Google Scholar, Medline, and Scopus (up to June 2024) to evaluate LFD's effects on global symptoms, gut microbiome, and related outcomes in IBS patients. Outcome data were expressed as standardized mean differences (SMD) and mean differences (MD). Quantitative synthesis was performed using random-effects or fixed-effect models.</div></div><div><h3>Result</h3><div>Ten RCTs were encompassed in this study. In comparison with the control diets (such as the traditional diet and high FODMAPs diet), the LFD resulted in significant symptom relief (IBS-SSS score: <em>n</em> = 286; <em>p</em> = 0.002; MD = −44.99, 95 % CI -74.05 to −15.91, <em>I</em><sup><em>2</em></sup> = 54 %), a higher response rate, and a notable enhancement in quality of life. Abdominal pain severity manifested significant improvement. Regarding bowel habits, the frequency of defecation decreased conspicuously, and patient satisfaction with defecation habits increased.</div></div><div><h3>Conclusion</h3><div>LFD effectively mitigates gastrointestinal symptoms and improves quality of life in IBS patients versus control diets. Further investigations should evaluate long-term effects on nutritional status and gut microbiota.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107190"},"PeriodicalIF":4.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-06-27DOI: 10.1016/j.fbio.2025.107157
Yehua Bao , Xiaoting Man , Meijuan Li , Yiwei Du , Jiayi Zhang , Jie Li , Xizeng Feng
{"title":"Erythritol disrupts neurodevelopmental signaling and behavior in zebrafish via shh/nodal and notch pathways","authors":"Yehua Bao , Xiaoting Man , Meijuan Li , Yiwei Du , Jiayi Zhang , Jie Li , Xizeng Feng","doi":"10.1016/j.fbio.2025.107157","DOIUrl":"10.1016/j.fbio.2025.107157","url":null,"abstract":"<div><div>The safety of erythritol, a widely used zero-calorie sugar substitute, concerning neurodevelopment is yet to be fully understood. In this study, we utilized EmbryoNet, a deep learning-driven embryonic phenotyping platform, to assess the neurodevelopmental toxicity of erythritol at concentrations of 3, 5, and 7 mg/mL, which adhere to FDA standards. Our findings demonstrated specific disruptions in the <em>shh</em> and <em>nodal</em> signaling pathways, critical regulators of vertebrate neural patterning. Experimental validation revealed that erythritol exposure led to microcephaly, reduced neural stem cells, and increased brain cell apoptosis in larvae. Mechanistically, reduced <em>ddx3x</em> gene expression in zebrafish inhibited the expression of Notch ligands (<em>dll4</em>, <em>jag1a</em>) and receptors (<em>notch1b</em>, <em>notch2</em>) by disrupting their interactions with <em>crebbp</em>, thereby triggering a range of effects. These include neurotransmitter disorders such as a GABA/glutamate imbalance (up-regulation of <em>gad1b</em> and down-regulation of <em>gabra2a</em> expression) and dopamine metabolic abnormalities (up-regulation of <em>dat</em> and down-regulation of <em>comt</em> gene expression). Behavioral analyses further corroborated these findings, showing erythritol-induced abnormalities including heightened thigmotaxis, stereotyped repetitive behaviors, and increased locomotor activity. These findings highlight the potential of erythritol to induce neurodevelopmental impairments through multifaceted interactions, implicating both autism-related genes (<em>nomo1</em>, <em>shank3a</em>, <em>shank3b</em>, <em>tanc2a</em>, <em>tanc2b</em>) and ADHD-related genes (<em>lphn3.1</em>, <em>lphn3.2</em>, per<em>1b</em>). This study underscores the harmful neurodevelopmental consequences of erythritol and establishes a framework for its rational and safe utilization.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107157"},"PeriodicalIF":4.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-06-27DOI: 10.1016/j.fbio.2025.107158
Bingbing Zhang , Huaijing Zhu , Lihui Zhang , Ming Luo , Xiaohua Li , Dahui Liu
{"title":"Evaluation of quality characteristics of 15 Gastrodia elata germplasm resources based on HS-SPME-GC-MS, HPLC and electronic nose techniques","authors":"Bingbing Zhang , Huaijing Zhu , Lihui Zhang , Ming Luo , Xiaohua Li , Dahui Liu","doi":"10.1016/j.fbio.2025.107158","DOIUrl":"10.1016/j.fbio.2025.107158","url":null,"abstract":"<div><div>As a medicinal-food homologous plant, <em>Gastrodia elata</em> (<em>G. elata</em>) possesses significant economic and medicinal value; however, current germplasm identification methods remain relatively simplistic. This study integrated High-Performance Liquid Chromatography (HPLC), Electronic Nose (E-nose), and Headspace Solid-Phase Microextraction coupled with Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS) to comprehensively analyze the phenolic and flavor profiles of 15 <em>G</em>. <em>elata</em> germplasms from China. HPLC analysis identified three key phenolic markers: gastrodin (0.05 %–0.64 %), 4-hydroxybenzylalcohol (with significant regional differences), and parishin E (0.57 %–1.26 %). E-nose analysis revealed that odor differences between <em>G. elata</em> germplasm were primarily detected by the W1W (Sensor response: 29.65), W2W (Sensor response: 11.18), and W5S (Sensor response: 12.11) sensors. Principal component analysis (PCA) effectively distinguished the 15 samples from 8 regions. HS-SPME-GC-MS analysis identified 43 characteristic volatiles. 4-Methoxymethylphenol (0 %–0.28 %), (E)-3-phenylprop-2-enoic acid (0 %–1.37 %), cyclododecane (0 %–0.31 %), undecylcyclopentane (0 %–0.60 %), and 3-methylheptadecane (0 %–0.95 %) emerged as discriminative markers for germplasm differentiation, while hexadecanoic acid (7.84 %–16.71 %) identified as a universal aroma component. Furthermore, this study revealed unique differential components in various <em>G. elata</em> variants, such as (Z,Z)-octadeca-9,12-dienoic acid (3.80 %–6.78 %) in <em>G. elata</em> Bl. <em>f. elata,</em> 2,4-di-tert-butylphenol (1.48 %–10.09 %) and methyl hexadecanoate (0.53 %–2.74 %) in <em>G. elata</em> Bl. <em>f. viridis</em>. By establishing a multidimensional identification framework for <em>G. elata</em>, this study elucidates the geo-chemical relationships and provides a scientific foundation for quality assessment, authenticity verification, and cultivar selection.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107158"},"PeriodicalIF":4.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food BiosciencePub Date : 2025-06-27DOI: 10.1016/j.fbio.2025.107162
Guijiang Liang , Wenpu Chen , Qiuming Chen , Zhaojun Wang , Zhiyong He , Maomao Zeng , H. Douglas Goff , Jie Chen
{"title":"Effect of NaCl on gel mechanical behavior of bovine serum albumin–soy protein blends","authors":"Guijiang Liang , Wenpu Chen , Qiuming Chen , Zhaojun Wang , Zhiyong He , Maomao Zeng , H. Douglas Goff , Jie Chen","doi":"10.1016/j.fbio.2025.107162","DOIUrl":"10.1016/j.fbio.2025.107162","url":null,"abstract":"<div><div>Soy protein gels are widely used in the food industry but have weak structures, even at high concentrations, limiting their functionality. This study explored how blending bovine serum albumin (BSA) with soy protein isolate (SPI) at different ratios and NaCl concentrations affects rheology, mechanical properties, water-holding capacity (WHC), and microstructure. Protein profile and particle size analyses revealed that moderate NaCl concentrations (50 mM) enhanced protein aggregation by reducing electrostatic repulsion and exposing reactive sites essential for gel network formation. Textural analysis confirmed that BSA-dominant gels exhibited higher hardness, springiness, and chewiness, whereas SPI-dominant gels formed weaker networks. At a 7:3 BSA: SPI ratio with 50 mM NaCl, gel hardness increased 1.4-fold higher than the no-NaCl control, reaching a peak of 5142 g. However, increasing NaCl to 100 mM caused over-aggregation and structural heterogeneity, reducing WHC by 8 % (from 95.2 % to 87.3 %) in pure BSA gels. The sample structure with 7:3 BSA: SPI ratio and 100 mM NaCl maintained its strength and consistency, with the storage modulus (G') increasing 1.3-fold compared to BSA-only gels. This treatment achieved an optimal balance of protein interactions, network stability, and textural properties. Intermolecular force disruption experiments demonstrated that disulfide bonds and hydrophobic interactions dominated BSA-rich gels. In contrast, hydrogen bonding and electrostatic interactions played a more significant role in SPI-rich gels. These findings indicate that optimizing BSA: SPI ratios and NaCl concentrations can be applied to achieve the desirable gel texture, strength, and moisture retention, which can be used in developing tailored protein-based food systems.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"71 ","pages":"Article 107162"},"PeriodicalIF":4.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}