Food Bioscience最新文献

{"title":"In Situ UDP-GlcA Generation Enables Glucuronide-Selective Multienzyme Biosynthesis and Structural Discovery of Quercetin Glucuronides","authors":"Wenjunjie Li, Shikang Chen, Qiuming Chen, Zhaojun Wang, Maomao Zeng, Jie Chen, Zhiyong He","doi":"10.1016/j.fbio.2026.110044","DOIUrl":"10.1016/j.fbio.2026.110044","url":null,"abstract":"<div><ul><li><span>•</span><span><div>GlcA-driven cascade enabled in situ UDP-GlcA supply for Q3G synthesis.</div></span></li><li><span>•</span><span><div>Quercetin-3,7-O-diglucuronide was enzymatically synthesized for the first time.</div></span></li><li><span>•</span><span><div>M1 VvGT5 produced 1,162 mg/L Q3G with 81.0% quercetin conversion.</div></span></li><li><span>•</span><span><div>Quercetin glucuronides showed improved simulated intestinal retention.</div></span></li></ul></div><div><div>Quercetin-3-O-glucuronide (Q3G) is a bioactive quercetin metabolite with potential applications in functional foods and nutraceuticals. However, its low natural abundance and reliance on UDP-glucuronic acid (UDP-GlcA) hinder scalable production. Here, we developed a three-enzyme cascade that couples UDP-GlcA generation from GlcA with VvGT5-catalyzed quercetin glucuronidation. Integrated sequence and expression optimization enhanced soluble VvGT5 expression, while AiCE-guided engineering generated the variant M1 (M189L/P251T/R307P). M1 exhibited 1.63-fold higher apparent activity, improved thermostability, and a 1.42-fold increase in apparent kcat, with kcat/Km remaining nearly unchanged. Under optimized 1-L cascade conditions, M1 produced 1,162 mg/L Q3G from 3 mM quercetin, corresponding to 81.0% conversion. Quercetin-3,7-O-diglucuronide was also enzymatically synthesized for the first time and structurally confirmed by MS and multidimensional NMR. Compared with quercetin, Q3G and quercetin-3,7-O-diglucuronide showed 2.9- and 3.1-fold higher residual levels after simulated intestinal digestion, respectively, while retaining appreciable ABTS and DPPH radical-scavenging activities. These findings establish an efficient in situ UDP-GlcA supply strategy for producing structurally defined quercetin glucuronides and highlight their potential as bioaccessible flavonoid derivatives for food-related applications.</div></div><div>Quercetin-3-O-glucuronide (Q3G) is a bioactive quercetin metabolite with potential applications in functional foods and nutraceuticals. However, its low natural abundance and reliance on UDP-glucuronic acid (UDP-GlcA) hinder scalable production. Here, we developed a three-enzyme cascade that couples UDP-GlcA generation from GlcA with VvGT5-catalyzed quercetin glucuronidation. Integrated sequence and expression optimization enhanced soluble VvGT5 expression, while AiCE-guided engineering generated the variant M1 (M189L/P251T/R307P). M1 exhibited 1.63-fold higher apparent activity, improved thermostability, and a 1.42-fold increase in apparent kcat, with kcat/Km remaining nearly unchanged. Under optimized 1-L cascade conditions, M1 produced 1,162 mg/L Q3G from 3 mM quercetin, corresponding to 81.0% conversion. Quercetin-3,7-O-diglucuronide was also enzymatically synthesized for the first time and structurally confirmed by MS and multidimensional NMR. Compared with quercetin, Q3G and quercetin-3,7-O-diglucuronide showed 2.9- and 3.1-fold hig","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"85 ","pages":"Article 110044"},"PeriodicalIF":6.2,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149006514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional strategies for combating sarcopenia: The metabolic regulatory activities and molecular mechanisms of multi-component dietary factors","authors":"Yu Liang,&nbsp;Jie Zhang,&nbsp;Qiaocui Liu,&nbsp;Yan Li,&nbsp;Ningning Zhao,&nbsp;Tao Hou","doi":"10.1016/j.fbio.2026.109976","DOIUrl":"10.1016/j.fbio.2026.109976","url":null,"abstract":"<div><h3>Background</h3><div>Sarcopenia, an age-related decline in muscle mass, strength, and function, poses a major public health challenge due to global aging. Nutritional intervention represents a promising strategy owing to its favorable safety profile and multi-target potentials.</div></div><div><h3>Objective and Methods</h3><div>This review systematically summarizes the pathogenesis of sarcopenia and discusses the evidence and mechanisms of multi-component dietary interventions.</div></div><div><h3>Main Content and Findings</h3><div>The pathogenesis of sarcopenia includes protein homeostasis imbalance, mitochondrial dysfunction, oxidative stress, and chronic inflammatory responses. Protein homeostasis imbalance involves key signaling pathways including mTOR, AMPK, FoxO, MAPK, and Myostatin/SMAD. Proteins and amino acids promote anabolism by providing substrates and activating the mTOR pathway. n-3 polyunsaturated fatty acids exerted their effects primarily through anti-inflammatory actions and improving mitochondrial function. Vitamins such as vitamin D and vitamin K play critical roles in regulating satellite cell function, protein homeostasis, and antioxidant defense. Minerals including zinc, selenium and magnesium act as key cofactors, participating in signal transduction and antioxidant defense. Plant-derived polyphenols, terpenoids and alkaloids achieve anti-inflammatory, antioxidant effects and metabolic improvement by regulating pathways such as SIRT1/PGC-1α and NF-κB. Additionally, multiple dietary factors could achieve multi-targeted regulation of sarcopenia through synergistic effects.</div></div><div><h3>Conclusion and Perspectives</h3><div>This review systematically reveals the enormous potential of multicomponent dietary factors in combating sarcopenia through multi-target and multi-level molecular networks. Future research should focus on exploring the synergistic effects among different dietary components to provide personalized precision nutrition strategies for the prevention and treatment of sarcopenia.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"85 ","pages":"Article 109976"},"PeriodicalIF":6.2,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149000667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus coagulans 217 combined with β-glucan reduces weight gain in high-fat-diet-fed mice","authors":"Yalin Zhang, Jie Liang, Yanmei Lu, Chen Yang, Junwei Zeng, Ruize Xu, Baoyue Cui, Zhenshang Xu, Ting Wang","doi":"10.1016/j.fbio.2026.109853","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.109853","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"84 1","pages":"109853-109853"},"PeriodicalIF":0.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomic insights into exopolysaccharide biosynthesis in Lactiplantibacillus plantarum induced by high-pressure processing and ultrasound: Enhanced yield, structural remodeling, and antioxidant capacity","authors":"Meiling Zhao, Jun Yuan, Yujie Zhong, Zhijia Liu, Chuanqi Chu, Junjie Yi, Tao Wang","doi":"10.1016/j.fbio.2026.109857","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.109857","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"84 1","pages":"109857-109857"},"PeriodicalIF":0.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-exposure to polyethylene microplastics and tetracycline alters nutritional quality, antioxidant capacity, amino acid profiles, and cultivation-associated microbiota of soybean sprouts","authors":"Lisha Deng, Wenjiang Zhou, Xiuzheng Zheng, Xuepeng Jin, Hongmei Jiang, Lanchai Chen, Jie Tang, Wenliang Xiang, Ting Cai","doi":"10.1016/j.fbio.2026.109829","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.109829","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"84 1","pages":"109829-109829"},"PeriodicalIF":0.0,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of triglyceride structure on lipid digestibility and bioaccessibility of β-carotene during in vitro digestion of oleogels","authors":"Jialing Long, Suyuan Zhao, Yingying Zhang, Dayang Zhu, Mingming Zheng, Yibin Zhou, Shiyi Li","doi":"10.1016/j.fbio.2026.109818","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.109818","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"84 1","pages":"109818-109818"},"PeriodicalIF":0.0,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Lactiplantibacillus plantarum KLDS1.0386 and Bifidobacterium longum subsp. longum EASY1813 alleviate CUMS-induced depressive-like behaviors through modulation of bile acid metabolism","authors":"Kangyong Zhang,&nbsp;Junwu Cai,&nbsp;Shikai Zhao,&nbsp;Fei Liu,&nbsp;Bailiang Li,&nbsp;Yupeng Wang","doi":"10.1016/j.fbio.2026.109801","DOIUrl":"10.1016/j.fbio.2026.109801","url":null,"abstract":"<div><div>Gut microbiota is regarded as a key hub linking diet, microbial metabolism, and emotional homeostasis. The development of gut-brain axis-based combined probiotic intervention strategies provides a new perspective for the nutritional regulation of chronic stress-related mood disorders. In this study, a mouse model of depression induced by chronic unpredictable mild stress (CUMS) was used to evaluate the antidepressant-like effects and potential mechanisms of <em>L. plantarum</em> KLDS1.0386 combined with <em>B. longum</em> subsp. <em>longum</em> EASY1813. Compared with the individual strains, the combined intervention produced the most pronounced overall improvement in CUMS-induced depression-like phenotypes, as evidenced by enhanced spontaneous locomotor activity and exploratory behavior, increased sucrose preference, and reduced immobility in the tail suspension and forced swimming tests. This superior behavioral improvement was accompanied by greater normalization of HPA-axis hyperactivation and neurofunction-related indicators, including DA, NE, 5-HT, and BDNF, together with attenuation of hippocampal neuronal damage. Further analyses combining 16S rRNA sequencing and untargeted metabolomics revealed that the combined intervention promoted the enrichment of potentially beneficial bacteria, reshaped the gut microbial structure, and modulated primary bile acid biosynthesis as well as TUDCA-related metabolic alterations. Collectively, these findings demonstrate that combined KLDS1.0386 and EASY1813 intervention exerted more pronounced antidepressant-like effects than either strain alone and suggest the potential involvement of a gut microbiota-bile acid metabolism-neural function axis.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"84 ","pages":"Article 109801"},"PeriodicalIF":6.2,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148780161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green extraction and foodomics-based profiling of Grindelia robusta Nutt. as a functional food ingredient: Evaluation of chemopreventive potential via dual carbonic anhydrase inhibition and antiproliferative activity against triple-negative breast cancer","authors":"Nihan BOZKURT,&nbsp;Yakup BUDAK","doi":"10.1016/j.fbio.2026.109798","DOIUrl":"10.1016/j.fbio.2026.109798","url":null,"abstract":"<div><div><em>Grindelia robusta</em> Nutt. is a traditional medicinal plant widely used in North America to treat respiratory ailments such as bronchitis and asthma, with its traditional use officially recognized by the European Medicines Agency. Despite its ethnobotanical importance, the metabolic diversity and potential against modern pharmacological targets, including human carbonic anhydrase (hCA) isoforms, remain largely unexplored. This study aimed to optimize green extraction from different aerial parts (flowers, leaves, and stems) of <em>G. robusta</em> and to evaluate their phytochemical profiles, inhibitory effects on hCA isoforms, and antiproliferative activity against triple-negative breast cancer cells. Ultrasound-assisted extraction (UAE), Soxhlet extraction, and maceration were compared. Phytochemical characterization was performed using HPLC-DAD and UHPLC-QTOF-MS/MS. Biological activities were assessed through enzyme inhibition kinetics, molecular docking, and cytotoxicity assays on MDA-MB-231 and MCF-10A cell lines. UAE proved to be the most efficient and environmentally friendly technique, yielding the highest extract from flowers (22.4 %) with low energy consumption (0.085 kWh/run). Foodomics-based untargeted metabolomic profiling revealed a high abundance of diterpenoids, particularly in flower extracts. The flower extract exhibited potent inhibition of tumor-associated hCA IX (IC₅₀ = 42.15 μg/mL) and hCA XII (IC₅₀ = 36.42 μg/mL), as well as hCA II (IC₅₀ = 82.4 ± 3.9 μg/mL). In addition, it showed selective antiproliferative activity against MDA-MB-231 cells (IC₅₀ = 154.3 ± 5.8 μg/mL after 24 h), with a selectivity index of 1.92, and induced mitochondria-mediated apoptosis. These results provide preliminary scientific support for the traditional ethnobotanical use of G. robusta and highlight its potential as a botanical source of natural carbonic anhydrase inhibitors with moderate antiproliferative selectivity against TNBC cells. The integration of green extraction technologies with high-resolution metabolomics positions <em>G. robusta</em> as a candidate nutraceutical ingredient, warranting further in vivo validation.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"84 ","pages":"Article 109798"},"PeriodicalIF":6.2,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148780158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic reinforcement of cellulose-based composite films by wheat bran cellulose nanocrystals and tea polyphenols for fruit preservation","authors":"Xiao Liu, Rumeng Li, Sa Cui, Wenjia Wang, Yiping Liang, Huijuan Zhang","doi":"10.1016/j.fbio.2026.109704","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.109704","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"83 1","pages":"109704-109704"},"PeriodicalIF":0.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular-weight modulation improves the physicochemical properties and in vitro release behavior of spray-dried Agaricus bisporus polysaccharide microcapsules","authors":"Simin Feng, Jiaxin Dai, Tingxuan Zong, Manyu Zhang, Pu Yang, Yuyan Wang, Kseniya Hileuskaya, Yizhen Xie, Ping Shao","doi":"10.1016/j.fbio.2026.109703","DOIUrl":"https://doi.org/10.1016/j.fbio.2026.109703","url":null,"abstract":"","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"83 1","pages":"109703-109703"},"PeriodicalIF":0.0,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}