Current advances in aptamer-functionalized DNA nanostructures for pathogenic bacteria detection in food

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-07-08 DOI:10.1016/j.fbio.2025.107218

Mengyue Liu , Giovanna Marrazza , Yemin Guo , Xia Sun , Shicai Xu

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Abstract

Pathogenic bacteria are widely present in food and the environment, posing a serious threat to human health. The key to addressing this issue lies in the development of rapid and effective sensors for the sensitive detection of pathogenic bacteria. Aptamer-functionalized DNA nanostructures represent a cutting-edge approach in biosensing, offering high specificity and versatility for pathogenic bacteria detection. By integrating synthetic aptamers with programmable DNA nanostructures, researchers are developing innovative tools that surpass traditional methods in speed, sensitivity, and portability. This review focuses on the design, types, and applications of aptamer-functionalized DNA nanostructures in the field of pathogenic bacteria detection. We provide a detailed analysis of the assembly methods, characteristics, and functionalities of these nanostructures. Additionally, this work summarizes the applications of aptamer-functionalized DNA nano-sensors based on various sensing signals. Finally, we discuss current challenges and future prospects to inspire advancements in this dynamic field.

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核酸适体功能化DNA纳米结构在食品致病菌检测中的研究进展

致病菌广泛存在于食品和环境中，对人类健康构成严重威胁。解决这一问题的关键在于开发快速有效的传感器来灵敏地检测病原菌。适配体功能化的DNA纳米结构代表了生物传感领域的一种前沿方法，为致病菌检测提供了高特异性和多功能性。通过将合成适配体与可编程DNA纳米结构相结合，研究人员正在开发在速度、灵敏度和便携性方面超越传统方法的创新工具。本文综述了核酸适体功能化DNA纳米结构的设计、类型及其在致病菌检测领域的应用。我们提供了这些纳米结构的组装方法，特性和功能的详细分析。此外，本文还总结了适配体功能化DNA纳米传感器在各种传感信号上的应用。最后，我们讨论了当前的挑战和未来的前景，以激发这一充满活力的领域的进步。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.