Food Bioscience最新文献_第2页

Targeting the two-component Agr system in Staphylococcus aureus: Molecular docking and dynamics insights into natural compound inhibition 靶向金黄色葡萄球菌双组分Agr系统：天然化合物抑制的分子对接和动力学见解

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-26 DOI: 10.1016/j.fbio.2025.107670

Praisy Joy Bell I, Rajiniraja Muniyan

{"title":"Targeting the two-component Agr system in Staphylococcus aureus: Molecular docking and dynamics insights into natural compound inhibition","authors":"Praisy Joy Bell I, Rajiniraja Muniyan","doi":"10.1016/j.fbio.2025.107670","DOIUrl":"10.1016/j.fbio.2025.107670","url":null,"abstract":"<div><div>The escalating prevalence of antimicrobial resistance, particularly in methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), necessitates the development of alternative therapeutic strategies beyond traditional antibiotics. Targeting quorum sensing (QS), a bacterial communication mechanism that modulates virulence, biofilm development, and toxin secretion represents a promising anti-virulence approach. In <em>S. aureus</em>, the Accessory gene regulator (Agr) system, comprising the response regulator AgrA and the sensor kinase AgrC, plays a pivotal role in pathogenicity regulation. In this study, a structure-based computational workflow was employed to identify food-derived quorum sensing inhibitors (QSIs) targeting AgrA and AgrC. A curated set of bioactive compounds from the FooDB database was subjected to ADMET profiling, molecular docking, molecular dynamics (MD) simulations, and MM-PBSA binding free energy calculations. Several candidate compounds exhibited strong binding affinities (ranging from −9 to −7.5 kcal/mol) and stable interactions with key residues of AgrA and AgrC. MD simulations over 100 ns confirmed the stability of these protein–ligand complexes, while Molecular Mechanics-Poisson-Boltzmann Surface Area (MM-PBSA) analysis and interaction fingerprint analysis indicated favourable binding energetics. Collectively, these findings suggest that food-derived compounds hold significant potential as safe and effective QSIs capable of disrupting <em>S. aureus</em> virulence pathways. This anti-virulence strategy offers a novel and resistance-mitigating therapeutic avenue by attenuating bacterial pathogenicity without exerting direct bactericidal pressure.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107670"},"PeriodicalIF":5.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevention and detoxification of mycotoxins in food and feed: A review 食品和饲料中真菌毒素的预防和解毒：综述

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-26 DOI: 10.1016/j.fbio.2025.107667

Kubra Deliklitas , Cengiz Gokbulut

{"title":"Prevention and detoxification of mycotoxins in food and feed: A review","authors":"Kubra Deliklitas , Cengiz Gokbulut","doi":"10.1016/j.fbio.2025.107667","DOIUrl":"10.1016/j.fbio.2025.107667","url":null,"abstract":"<div><div>Mycotoxins are toxic secondary metabolites produced by various species of fungi, including mainly <em>Fusarium</em> spp., <em>Aspergillus</em> spp., and <em>Penicillium</em> spp., which are found globally in different foods and animal feed. These substances present significant health risks to both humans and animals due to their carcinogenic, genotoxic, teratogenic, nephrotoxic, and hepatotoxic properties. These toxins contaminate food and feed products during the harvesting and storage process, affecting up to 50 % of the total and causing significant economic losses. The financial impact of this issue includes not only the costs associated with disposing of contaminated food but also a reduction in overall food productivity. The main types of mycotoxins that pose serious risks to both human and animal health include aflatoxins, fumonisins, ochratoxins, zearalenone, trichothecenes, ergot alkaloids and patulin. The impact of physical and chemical methods on food quality, their environmental toxicity, cost implications, and potential for leaving residues, as well as concerns about the consistency of production with biological processes, are among the limitations. Advances in biotechnology are expected to offer the greatest potential for future improvements. This review aims to highlight the latest techniques used to reduce or eliminate mycotoxins in food and feed materials using physical, chemical, and biological methods.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107667"},"PeriodicalIF":5.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking bioinformatics and structural insights of a novel thermo-alkaline pectate lyase ScpB from Phytophthora parasitica INRA-310 从疫霉INRA-310中提取的新型热碱性果胶裂解酶ScpB的生物信息学和结构分析

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107662

Mei Zhao , Xiaohong Pan , Jiaojiao Chen , Jie Shang , Yiwei Zhang , Cunsheng Zhang , Liang Guo , Xianghui Qi

{"title":"Unlocking bioinformatics and structural insights of a novel thermo-alkaline pectate lyase ScpB from Phytophthora parasitica INRA-310","authors":"Mei Zhao , Xiaohong Pan , Jiaojiao Chen , Jie Shang , Yiwei Zhang , Cunsheng Zhang , Liang Guo , Xianghui Qi","doi":"10.1016/j.fbio.2025.107662","DOIUrl":"10.1016/j.fbio.2025.107662","url":null,"abstract":"<div><div>Pectate lyase (PEL) has broad industrial utility, but its application is limited by insufficient thermal stability and alkali tolerance. In this study, a novel PEL (hereafter, ScpB) from <em>Phytophthora parasitica</em> INRA-310 was identified for the first time through data-driven mining and bioinformatics. Homology modeling revealed that ScpB consists of two domains: a PEL superfamily-associated domain ScpB-I and a catalytic domain ScpB-II, which are connected by a short linker region. Based on truncated engineering and protein structure engineering strategies, ScpB-I and ScpB-II must cooperate to exhibit the PEL activity. Furthermore, ScpB was recruited for developing biochemical properties. The purified ScpB exhibited optimal activity at pH 10.0 and 55 °C, aligning with textile biorefining conditions. Notably, in the presence of Ca<sup>2+</sup> and Fe<sup>3+</sup>, a 215.97 % and 182.08 %, respectively, improvement in catalytic activity compared to the control group without metal ion. Finally, the bioscouring assay was carried out, ScpB exhibited a significant increase in the wetted fabric area to 5.371 ± 0.382 cm<sup>2</sup>, which was 3.6 times that of the untreated sample (1.496 cm<sup>2</sup>). Scanning electron microscopy confirmed effective pectin removal and smoother fibers. This study expands the PEL resource library and offers a promising enzyme for efficient, eco-friendly textile biorefining.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107662"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis and formation mechanism of Key aroma compounds with ammonia-like off-flavors in Jiang-flavored high-temperature daqu: Substances composition and main microorganisms 江味高温大曲中含氨类异味的关键香气化合物及其形成机理分析：物质组成及主要微生物

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107656

Ping Tang , Qingliang Li , Changwen Li , Dongguang Xiao , Xiaodan Wang , Xuewu Guo

{"title":"Analysis and formation mechanism of Key aroma compounds with ammonia-like off-flavors in Jiang-flavored high-temperature daqu: Substances composition and main microorganisms","authors":"Ping Tang , Qingliang Li , Changwen Li , Dongguang Xiao , Xiaodan Wang , Xuewu Guo","doi":"10.1016/j.fbio.2025.107656","DOIUrl":"10.1016/j.fbio.2025.107656","url":null,"abstract":"<div><div>Ammonia odor is a notable off-flavor in Jiang-flavored high-temperature Daqu, impacting its quality, but the substances composition and formation of this odor are still unknown. In this study, molecular sensory science was employed to identify the key flavor compounds responsible for the ammonia odor in Daqu, while microbiomic analysis, correlation analysis, and functional gene prediction were conducted to investigate the mechanisms of their formation. Trimethylamine was identified as the predominant component contributing to the ammonia odor characteristic of Daqu. Subsequent microbial community analysis revealed that <em>Bacillus</em>, <em>Kroppenstedtia</em>, <em>Thermoascus</em>, and <em>Thermomyces</em> were the dominant microorganisms differentiating ammonia-flavored Daqu (AFDQ) from non-ammonia-flavored Daqu (NFDQ). Moreover, significant variations in the amino acid metabolic pathways of the bacterial community functional genes between these two Daqu types further influenced the development of the ammonia flavor. Concurrently, the research findings were applied to the Daqu production process for the identification of AFDQ. A support vector machine (SVM) discrimination model was developed based on trimethylamine and total volatile basic nitrogen levels, demonstrating robust performance in distinguishing AFDQ from NFDQ. These findings provide new insights into the flavor formation mechanisms of ammonia-flavored Daqu and offer a scientific basis for its quality control and optimization.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107656"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weissella cibaria X1 fermentation enhances the UA-lowering ability of Carthamus tinctorius L. via metabolic regulation Weissella cibaria X1发酵通过代谢调节提高红花（Carthamus tintorius L.）降ua能力

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107643

Xiqian Tan, Anqi Sun, Shuaibo Gao, Zixiao Shen, Lijun You, Fangchao Cui, Xuepeng Li, Jianrong Li

{"title":"Weissella cibaria X1 fermentation enhances the UA-lowering ability of Carthamus tinctorius L. via metabolic regulation","authors":"Xiqian Tan, Anqi Sun, Shuaibo Gao, Zixiao Shen, Lijun You, Fangchao Cui, Xuepeng Li, Jianrong Li","doi":"10.1016/j.fbio.2025.107643","DOIUrl":"10.1016/j.fbio.2025.107643","url":null,"abstract":"<div><div>The uric acid (UA) degrading <em>Weissella cibaria</em> X1 was employed for the fermentation of safflower (<em>Carthamus tinctorius</em> L.). Compared with naturally fermented safflower, X1 fermented safflower significantly enhancing the antioxidant capacity and xanthine oxidase (XO) inhibition rate, decreased UA levels by 81.30 %, XO activity by 99.02 %, and creatinine levels by 27.08 % based on a hyperuricemia (HUA) zebrafish model. Additionally, it upregulated the genes inhibiting UA synthesis—hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1 gene) 1.91 fold, and those promoting UA excretion—urate oxidase (UOX) gene 5.40 fold, organic anion transporter 1 (OAT1) gene 4.80 fold, hepatocyte nuclear factor 4 alpha (HNF4A gene) 3.29 fold, and polyvalent PDZ domain 1 (PDZK1) gene 57.46 fold. Subsequently, non-targeted metabolomics analysis showed that X1 fermentation downregulated the content of L-glutamine and modulated purine metabolism than the safflower fermented naturally, thus decreasing the levels of purines and increasing those of scolymoside. Our research provided a potential HUA therapy via both pharmaceutical and nutraceutical strategies.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107643"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex amplification assisted real-time nanopore accurate sequencing for rapid and on-site identification of Pseudomonas aeruginosa in packaged drinking water 多重扩增辅助实时纳米孔精确测序用于包装饮用水中铜绿假单胞菌的快速和现场鉴定

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107658

Xianzhuo Meng , Qi Chen , Bangben Yao , Jiening Yang , Zhaoran Chen , Samuel B. Adeloju , Wei Chen

{"title":"Multiplex amplification assisted real-time nanopore accurate sequencing for rapid and on-site identification of Pseudomonas aeruginosa in packaged drinking water","authors":"Xianzhuo Meng , Qi Chen , Bangben Yao , Jiening Yang , Zhaoran Chen , Samuel B. Adeloju , Wei Chen","doi":"10.1016/j.fbio.2025.107658","DOIUrl":"10.1016/j.fbio.2025.107658","url":null,"abstract":"<div><div>The occurrence of <em>Pseudomonas aeruginosa (P. aeruginosa)</em> contamination in packaged drinking water has become a significant public health concern. Accurate, specific and rapid detection of <em>P. aeruginosa</em> in drinking water is crucial to safeguard public health. In this study, we developed a multiplex PCR assisted real-time nanopore sequencing method for rapid and accurate identification of target <em>P. aeruginosa</em>. Three primer sets of long amplicons with non-overlapping sequences were designed. By combining real-time sequencing, the time-varying current signals captured by nanopore proteins were used for real-time identification of the sequence information of the measured strand using the portable nanopore gene sequencing device. Rapid and on-site identification of <em>P. aeruginosa</em> in packaged drinking water were achieved through real-time bioinformatics analysis. The total time of sequence-based identification could be completed in less than 220 min from sampling to the final results output. Interestingly, with our designed multiplex PCR assisted strategy, the core sequencing operations were completed in just 5 min, and the detection limit could be achieved as 10<sup>2</sup> CFU/250 mL without pre-culture treatment. Of greater significance, this multiplex PCR assisted real-time sequencing strategy can be applied for the identification of other common bacteria by designing corresponding long amplicon primer sets, making it a potential universal method for rapid and accurate bacteria identification in food safety.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107658"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secretory expression of κ-carrageenase MtKC16A in Bacillus subtilis for the preparation of κ-carratriose κ-卡拉胶酶MtKC16A在枯草芽孢杆菌中分泌性表达制备κ-卡拉胶

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107663

Chengcheng Jiang , Xiangzhao Mao

{"title":"Secretory expression of κ-carrageenase MtKC16A in Bacillus subtilis for the preparation of κ-carratriose","authors":"Chengcheng Jiang , Xiangzhao Mao","doi":"10.1016/j.fbio.2025.107663","DOIUrl":"10.1016/j.fbio.2025.107663","url":null,"abstract":"<div><div>Carrageenases can degrade carrageenan and produce even-numbered neocarrageenan-oligosaccharides (NCOSs). However, the gel properties of κ-carrageenan limit the maximum concentration of substrate that can be treated by κ-carrageenase. In this study, we first constructed an expression system in <em>Bacillus subtilis</em> and achieved secretion of Nκ4-producing κ-carrageenase MtKC16A, with an activity of 0.016 U/mL. Our further exploration showed that MtKC16A can also cleave the trisaccharide unit from the non-reducing end of κ-carraheptaose, κ-carranonaose, κ-carraundecaose, and κ-carrauntetradecaose. This allowed us to successfully prepare κ-carratriose by combining acid hydrolysis and enzymatic hydrolysis. We were able to obtain 0.47 ± 0.01 g of κ-carratriose from 1 g of κ-carrageenan, with a high yield of 47.0 % (w/w). Furthermore, our study demonstrated that carrageenanoligosaccharides (COSs) prepared by acid hydrolysis combined with enzymatic hydrolysis have excellent <em>in vitro</em> antioxidant activity, significantly higher than polysaccharides. This study presents new methods for the efficient preparation of odd-numbered COSs using κ-carrageenases.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107663"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-level production of L-methionine through collaborative modification of multiple modules in Escherichia coli 通过大肠杆菌中多个模块的协同修饰高水平生产l -蛋氨酸

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107664

Kun Niu , Mao-Qin Chen , Qiao-Ling Zhang , Zi-Xuan Zhang , Wen-Bin Cai , Zhi-Qiang Liu , Yu-Guo Zheng

{"title":"High-level production of L-methionine through collaborative modification of multiple modules in Escherichia coli","authors":"Kun Niu , Mao-Qin Chen , Qiao-Ling Zhang , Zi-Xuan Zhang , Wen-Bin Cai , Zhi-Qiang Liu , Yu-Guo Zheng","doi":"10.1016/j.fbio.2025.107664","DOIUrl":"10.1016/j.fbio.2025.107664","url":null,"abstract":"<div><div>L-Methionine is the only sulfur-containing essential amino acid, and it plays a pivotal role in various industries. Due to the unique sulfur-containing structure and complex synthetic regulation, the biological production of L-methionine is still difficult to rival with the chemical synthesis method. In this study, L-cysteine synthesis and one-carbon unit supply modules were successively modified based on a non-auxotrophic L-methionine producing strain. Strengthening the genes in sulfate pathway effectively boosted L-methionine production to 2.85 g/L, and the reduction of SO<sub>4</sub><sup>2−</sup> to SO<sub>3</sub><sup>2−</sup> and the supply of cofactors for sulfite reductase were key limitations for sulfur assimilation in this pathway. And the modification of the thiosulfate, new thiosulfate, and the one-carbon unit yielded no significant results. However, improving the cell membrane permeability could enhance the L-methionine production to 3.45 g/L. Finally, the L-methionine production reached 36.06 g/L for fed-batch fermentation in a 5-L bioreactor, which was the highest reported L-methionine titer to date. The study provides a well research foundation for L-methionine production by microbial fermentation with the capacity for industrial application.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107664"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving phenyllactic acid production of Gluconacetobacter tumulisoli FBFS 97 using a strong promoter, Pkan 使用强启动子Pkan改善葡醋杆菌坟坟FBFS 97的苯乳酸生成

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107659

Yijun Liu , Yanqin Ma , Jiayun Song , Kyay Mohn Thinn , Fusheng Chen

{"title":"Improving phenyllactic acid production of Gluconacetobacter tumulisoli FBFS 97 using a strong promoter, Pkan","authors":"Yijun Liu , Yanqin Ma , Jiayun Song , Kyay Mohn Thinn , Fusheng Chen","doi":"10.1016/j.fbio.2025.107659","DOIUrl":"10.1016/j.fbio.2025.107659","url":null,"abstract":"<div><div>Phenyllactic acid (PLA) is a broad-spectrum antimicrobial substance with broad application prospects in the food industry. In our previous research, we isolated and screened a strain of acetic acid bacteria (AAB) from vinegar mash, <em>Gluconacetobacter tumulisoli</em> FBFS 97, which is the first reported AAB strain with the PLA production capacity. In current study, in order to increase the PLA production of the FBFS 97 strain, a strong promoter, P<sub><em>kan</em></sub> (promoter of kanamycin resistance gene), suitable for the FBFS 97 strain, was screened using enhanced green fluorescent protein (EGFP) as a reporter. Then five related genes with PLA biosynthesis in the FBFS 97 strain, including the chorismate synthase gene <em>aroc</em>, the prephenate dehydratase gene <em>pheA</em>, the glucose dehydrogenase gene <em>gdh</em>, the formate dehydrogenase gene <em>fdh</em> and the glycerate dehydrogenase gene <em>gldh</em> were overexpressed using the P<sub><em>kan</em></sub> promoter, respectively. The results revealed that except the <em>gldh</em> overexpression strain, PLA production of other mutants were improved to 125.9 %, 140.2 %, 130 % and 113 % comparing to that (56.85 mg/L) of the FBFS 97 strain, respectively. This study not only lays the foundation for constructing the high-producing PLA engineering strain using the FBFS 97, but also provides a novel insight for better understanding the PLA biosynthesis pathway of microorganisms.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107659"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a real-time PCR method for the detection of penicillin fermentation residue in feed 饲料中青霉素发酵残留物实时PCR检测方法的建立

IF 5.9 1区农林科学

Food Bioscience Pub Date : 2025-09-25 DOI: 10.1016/j.fbio.2025.107618

Yuhang Fan , Juan Zhang , Yang Li , Dengcheng Suo , Zengling Yang , Xiaoyu Li , Xia Fan , Ailiang Chen

{"title":"Development of a real-time PCR method for the detection of penicillin fermentation residue in feed","authors":"Yuhang Fan , Juan Zhang , Yang Li , Dengcheng Suo , Zengling Yang , Xiaoyu Li , Xia Fan , Ailiang Chen","doi":"10.1016/j.fbio.2025.107618","DOIUrl":"10.1016/j.fbio.2025.107618","url":null,"abstract":"<div><div>Feed safety is critical to the quality of livestock and poultry products and plays a key role in ensuring food safety and public health. To address the growing issue of illicit addition of antibiotic fermentation residues, such as penicillin mycelial waste, into feed, we developed a real-time PCR method targeting the <em>orf70c</em> gene, a specific marker within the biosynthetic gene cluster of industrial penicillin-producing strains. Specific primers and a probe were designed to enable accurate detection across nine types of feed. The assay requires no complex sample pretreatment and completes the entire process from DNA extraction to result within 2 h. It reliably detects as little as 0.5 % (w/w) of penicillin fermentation residue in feed. The method demonstrated high reproducibility, with inter-assay variation between 0.46∼0.55 % and intra-assay variation between 0.37∼0.69 %. To our knowledge, this is the first real-time PCR assay developed for detecting antibiotic fermentation residues in feed. The method provides a practical tool for feed safety monitoring and supports sustainable practices in the feed and animal production industries.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107618"},"PeriodicalIF":5.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0