Food Bioscience最新文献_第3页

Sequence optimization and heterologous expression of xanthine oxidase inhibitory peptides in Escherichia coli 大肠杆菌中黄嘌呤氧化酶抑制肽的序列优化和异源表达

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-08 DOI: 10.1016/j.fbio.2024.105080

{"title":"Sequence optimization and heterologous expression of xanthine oxidase inhibitory peptides in Escherichia coli","authors":"","doi":"10.1016/j.fbio.2024.105080","DOIUrl":"10.1016/j.fbio.2024.105080","url":null,"abstract":"<div><p>To solve the problem of low efficiency and purity in preparation of active peptides through enzymatic hydrolysis, the xanthine oxidase (XO) inhibitory peptides were optimized according to structure-activity relationship and a heterologous expression system for these peptides was constructed. The XO inhibitory peptide AEAWMWR (IC<sub>50</sub> = 1.76 mM), which exhibited enhanced activity, was obtained by optimizing AEAQMWR (IC<sub>50</sub> = 8.85 mM) in this research. The optimized peptide AEAWMWR exhibited approximately a 5-fold increase in activity compared to the template peptide AEAQMWR. The optimization results indicated that replacing the non-hydrophobic amino acids in the middle of the sequence with W or adding W to the C-terminal of the sequence effectively improved the activity of peptides. Additionally, to further achieve low-cost and rapid preparation of the peptides AEAQMWR and AEAWMWR, the recombinant plasmids containing fusion proteins of tandem repetitive peptides were designed and expressed in <em>Escherichia coli</em>. The recombinant peptides AEAQMWR (IC<sub>50</sub> = 8.19 mM) and AEAWMWR (IC<sub>50</sub> = 1.57 mM) exhibited significant activity. These results demonstrate that rational optimization and microbial synthesis of peptides can efficiently prepare bio-active peptides.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2212429224015104/pdfft?md5=a31be6a425c6b238d03b83410ada846a&pid=1-s2.0-S2212429224015104-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant protein LPxT-GYLEQ attenuates cognitive impairment by ameliorating oxidative stress in D-galactose-induced aging mice model 重组蛋白 LPxT-GYLEQ 通过改善 D-半乳糖诱导的衰老小鼠模型中的氧化应激减轻认知障碍

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-08 DOI: 10.1016/j.fbio.2024.105079

{"title":"Recombinant protein LPxT-GYLEQ attenuates cognitive impairment by ameliorating oxidative stress in D-galactose-induced aging mice model","authors":"","doi":"10.1016/j.fbio.2024.105079","DOIUrl":"10.1016/j.fbio.2024.105079","url":null,"abstract":"<div><p>Food-derived antioxidant peptides have been shown to have beneficial effects in scavenging excess free radicals. In this study, a novel multifunctional LPxTG-motif protein LPxT-GYLEQ was synthesized, and its molecular mechanism of alleviating cognitive impairment in a D-galactose (D-gal)-induced aging mice model was also investigated. The results confirmed the antioxidant effects of the LPxT-GYLEQ protein, which could scavenge excessive reactive oxygen species (ROS) in aging mice by regulating the c-Jun N-terminal kinase (JNK)/Nuclear factor erythroid 2-related factor 2 (Nrf2)/p38/Nuclear factor-k-gene binding (NF-κB) signal pathway, reduced the accumulation of β-amyloid protein (Aβ), restored the cognitive ability of mice, improved learning and memory behavior, effectively reduced the expression of inflammatory-related factors, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and increased the expression of anti-inflammatory factor interleukin-10 (IL-10). These may be related to the fact that the protein regulates the abundance of beneficial bacteria such as intestinal <em>Akkermansia Muciniphila</em> (<em>Akk</em>). All results suggest that the synthetic LPxT-GYLEQ protein may improve cognitive impairment and be a promising candidate for an anti-aging agent.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel antimicrobial peptide MP-4: Insights into its antimicrobial properties and intestinal regulation on E. coli-infected mice 新型抗菌肽 MP-4：其抗菌特性及其对大肠杆菌感染小鼠肠道调节的启示

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-07 DOI: 10.1016/j.fbio.2024.104840

{"title":"A novel antimicrobial peptide MP-4: Insights into its antimicrobial properties and intestinal regulation on E. coli-infected mice","authors":"","doi":"10.1016/j.fbio.2024.104840","DOIUrl":"10.1016/j.fbio.2024.104840","url":null,"abstract":"<div><p>The quest for novel, potent antimicrobial agents with low resistance potential poses a significant challenge for the advancement of the food and medical sectors. This study aimed to elucidate the antibacterial potency of antimicrobial peptides derived from koumiss in a murine model. Leveraging an antimicrobial peptide database, six peptides (MP-1 to MP-6) were meticulously predicted and screened for their antibacterial properties. These peptides were subsequently synthesized using chemical solid-phase methods and their antibacterial activities were rigorously validated. Remarkably, among the six peptides, MP-4 demonstrated a profound antibacterial effect against <em>E</em>. <em>coli</em>, achieving rapid bacterial eradication within 240 min. Flow cytometry analysis further corroborated its significant bactericidal activity. <em>In vivo</em> experiments conducted on mice infected with <em>E. coli</em> revealed that oral administration of MP-4 significantly ameliorated symptoms such as lethargy, anorexia, and weight loss. Additionally, it effectively reduced the colonic <em>E. coli</em> burden, attenuated inflammatory responses, and favorably modulated the intestinal microbiota composition. This study not only validates the robust antibacterial activity of the koumiss-derived antimicrobial peptide MP-4, but also underscores its potential therapeutic application in mitigating <em>E. coli</em> infections and promoting intestinal health.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142151303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cinnamic acid and its derivatives on dipeptidyl peptidase 4: Structure-activity relationship and mechanism of inhibition 肉桂酸及其衍生物对二肽基肽酶 4 的影响：结构-活性关系和抑制机制

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-07 DOI: 10.1016/j.fbio.2024.105076

{"title":"Cinnamic acid and its derivatives on dipeptidyl peptidase 4: Structure-activity relationship and mechanism of inhibition","authors":"","doi":"10.1016/j.fbio.2024.105076","DOIUrl":"10.1016/j.fbio.2024.105076","url":null,"abstract":"<div><p>Dipeptidyl peptidase 4 (DPP4), which breaks down glucagon-like peptide 1 (GLP-1), is closely associated with glucose metabolism, and the inhibition of this enzyme is one of the important targets for the treatment of diabetes. Traditionally cinnamon and bitter melon have been in wide use in diabetes treatment, and cinnamic acid (CIA) as its main ingredient is expected to be an ideal DPP4 inhibitor. <em>In vitro</em> inhibition experiments showed that CIA had the lowest IC<sub>50</sub> (33.56 ± 1.13 mM) compared to the other substances in the study, suggesting that it was more effective in inhibiting DPP4. Analyses showed that adding hydroxyl and methyl groups to CIA's aromatic ring reduced its effect on DPP4; CIA and its derivatives were inhibited in a mixed way. With the exception of ferulic acid (FA), CIA and its derivatives quenched the fluorescence of DPP4 via a static quenching mechanism. Thermodynamic parameters show that the binding of CIA (the most inhibitory compound) to DPP4 was spontaneous and driven by hydrogen bonding. Atomic force microscopy and circular dichroism spectroscopy analyses reveal that upon binding with DPP4, CIA underwent a conformational change. Molecular docking results highlight, while introducing hydroxyl and methoxy groups on the aromatic ring, the superior binding capacity of CIA diminished. The study confirms that CIA is an ideal inhibitor with the highest absolute value of binding energy (−5.8) and the lowest IC<sub>50</sub> compared to other substances. By clarifying the inhibition mechanism of DPP4,the study thus provides dietary guidance for diabetic patients.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular docking and transcriptomic analysis reveal the mechanism of myosin-derived peptides activating bitter receptor of hT2R1 分子对接和转录组分析揭示肌球蛋白衍生肽激活 hT2R1 苦味受体的机制

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-07 DOI: 10.1016/j.fbio.2024.105067

{"title":"Molecular docking and transcriptomic analysis reveal the mechanism of myosin-derived peptides activating bitter receptor of hT2R1","authors":"","doi":"10.1016/j.fbio.2024.105067","DOIUrl":"10.1016/j.fbio.2024.105067","url":null,"abstract":"<div><p>To better understand the bitterness effect and molecule mechanism of myosin-derived peptides activating bitter receptors, the interaction between myosin-derived peptides of dry-cured ham and bitter receptors was investigated by molecular docking and molecular dynamics simulation; the signal transduction mechanism of myosin-derived peptides was explored by HEK-293T cells using calcium imaging and transcriptomics analysis. Lower CDOCKER energy was observed during the interaction between myosin-derived peptides and hT2R1 by molecular docking compared with hT2R4, hT2R5, hT2R8, hT2R14 and hT2R16. Hydrogen bonds and hydrophobic interaction were the most important interaction forces which stabilized the interaction of hT2R1 and myosin-derived peptides. Compared with LEKEKSELK and TEELEEAKK, the RMSF values and EC<sub>50</sub> values of HVLATLGEK were lower, indicating that hT2R1 was more sensitive to HVLATLGEK stimulation. Transcriptomics and KEGG analyses showed that 767 differentially expressed genes were found and mainly involved in cAMP signaling pathway, neuroactive ligand-receptor interaction, calcium signaling pathway and MAPK signaling pathway after stimulating of HVLATLGEK. Protein-protein interaction network further demonstrated that DDIT3, FOS, FOSB, MYC, EGR1 and CCN2 were the key genes to connect the six functional clusters including ligand-receptor interaction and signal transduction.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-melanogenic effect of a novel oligosaccharide derived from almond on forskolin-stimulated melanogenesis in B16F10 melanoma cells 杏仁中提取的一种新型寡糖对 B16F10 黑色素瘤细胞中由福斯可林刺激的黑色素生成具有抗黑色素生成作用

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-07 DOI: 10.1016/j.fbio.2024.105013

{"title":"Anti-melanogenic effect of a novel oligosaccharide derived from almond on forskolin-stimulated melanogenesis in B16F10 melanoma cells","authors":"","doi":"10.1016/j.fbio.2024.105013","DOIUrl":"10.1016/j.fbio.2024.105013","url":null,"abstract":"<div><p>The application of natural products for regulating skin pigmentation is increasingly being acknowledged due to their security and proven efficacy. Almond (<em>Amygdalus communis</em> L.) is an edible nut plant with significant nutritional value and medicinal attributes. In this research, oligosaccharide (ACO-II-1) was extracted and isolated applying gel chromatography techniques from the aqueous extract of almond kernel. Structural characterization of ACO-II-1 was conducted using ESI-MS, methylation analysis and 1D/2D-NMR. The findings indicated that ACO-II-1 was composed of fructose and glucose and the glycosidic bond predominantly featured <em>β</em>-D-Fruf-(1 → 2)-<em>β</em>-D-Fru-(1 → 6)-<em>α</em>-D-Glcp-(1 → 1)-<em>α</em>-D-Glcp. Moreover, ACO-Ⅱ-1 possessed strong antioxidant activity and notable inhibitory effect on melanogenesis in forskolin (FSK)-induced B16F10 melanoma cells. Subsequent mechanism investigation was conducted and suggested that the <em>anti</em>-melanogenic properties of ACO-Ⅱ-1 might be attributed to its modulation of the Mitogen-activated protein kinases (MAPKs) and β-catenin signaling pathways. These results indicated that edible plant derived ACO-II-1 might be served as promising inhibitor of hyperpigmentation with potential application in pharmaceutical and skin-care sector.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating characteristic fingerprints and volatile flavor compound variations during the fermentation of Guangxi bamboo shoot using HS-GC-IMS and HS-SPME-GC-MS 利用 HS-GC-IMS 和 HS-SPME-GC-MS 研究广西竹笋发酵过程中的特征指纹和挥发性风味化合物变化

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-07 DOI: 10.1016/j.fbio.2024.105071

引用次数: 0

Lipid digestion profiles of pickering emulsion gels stabilized by β-glucans microgel particles 用β-葡聚糖微凝胶颗粒稳定的皮克林乳液凝胶的脂质消化曲线

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-07 DOI: 10.1016/j.fbio.2024.105069

引用次数: 0

Dietary heme-enriched Corynebacterium extract exerts health benefits by reshaping gut microbiota 膳食血红素富集棒状杆菌提取物通过重塑肠道微生物群对健康有益

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-06 DOI: 10.1016/j.fbio.2024.105062

{"title":"Dietary heme-enriched Corynebacterium extract exerts health benefits by reshaping gut microbiota","authors":"","doi":"10.1016/j.fbio.2024.105062","DOIUrl":"10.1016/j.fbio.2024.105062","url":null,"abstract":"<div><p>We previously reported that a heme-enriched <em>Corynebacterium</em> extract (hSCP) alleviated weight gain and lowered subcutaneous fat mass in a murine model. To understand the mechanisms underlying the beneficial effects of dietary hSCP, we investigated the histological and microbiological changes in the guts of mice after hSCP administration in this study. Dietary hSCP lowered serum triglyceride and cholesterol levels and restrained adipose tissue expansion in mice fed a normal diet (ND) or a high-fat diet (HFD). The colonic mucosa was prominently lined with acidic and neutral mucin-enriched goblet cells and Muc2 transcription was increased by dietary hSCP in HFD-fed mice. Given the nutritional content of hSCP, microbial shifts triggered by dietary hSCP were dissected from the cecum and colon of the mice. Bacterial community compositions in the luminal contents were significantly reshaped by hSCP supplementation in HFD-fed mice and also differed between intestinal loci, that is, the cecum and colon. Dietary hSCP enriched short-chain fatty acid-producing bacterial genera in ND-fed mice, while attenuating other mucin-degrading bacteria in HFD-fed mice. The addition of hSCP to a co-culture of a defined microbiota community promoted <em>Lactobacillus gasseri</em> proliferation compared with nine other species. An <em>in vitro</em> culture medium supplemented with hSCP increased bile salt hydrolase activity and cholesterol assimilation by <em>L. gasseri</em>, which is reminiscent of the beneficial effects of <em>Lactobacillus</em> spp. as cholesterol-lowering probiotics. Our findings demonstrate the potential of hSCP as a dietary supplement with beneficial effects on lipid metabolism and gut health.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2212429224014925/pdfft?md5=88d9a89574782eebe783672b90c48b74&pid=1-s2.0-S2212429224014925-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interesterified palm oil leads to distinct variation in the intestinal microbiota of mice 酯化棕榈油导致小鼠肠道微生物群发生明显变化

IF 4.8 1区农林科学

Food Bioscience Pub Date : 2024-09-06 DOI: 10.1016/j.fbio.2024.105032

{"title":"Interesterified palm oil leads to distinct variation in the intestinal microbiota of mice","authors":"","doi":"10.1016/j.fbio.2024.105032","DOIUrl":"10.1016/j.fbio.2024.105032","url":null,"abstract":"<div><p>Interesterified fat (IF) has been established as a substitute for trans-fat in ultra-processed foods. Preclinical studies have demonstrated that IF increases intestinal barrier permeability and bacterial translocation, but it is still unknown its impacts on the intestinal microbiota. Thus, this study aimed to evaluate if IF modulates the intestinal microbiota and the production of short-chain fatty acids (SCFAs) by the intestinal bacteria of male Swiss mice fed control or high-fat diet, with or without IF. Animals were divided into groups that received anormolipidic control diet (10% of calories from lipids) or high-fat diet (45% of calories from lipids) containing unmodified fat or IF, both obtained from palm oil: CT (Control diet); ICT (Interesterified Control diet); HF (High-fat diet); IHF (Interesterified High-fat diet) for eight weeks. Fecal samples were collected directly from the animals' colons for processing. The SCFA production was analyzed by gas chromatography. For microbiota diversity, bioinformatics analysis was carried out. Significant differences in microbiota composition at the phylum level were not observed between diet groups. However, a reduction in abundance of the <em>Lactobacillus</em> and <em>Enterococcus</em> genera, and an increase in the <em>Peptococcus</em> genus were found in high-fat diet groups. Interestingly, in the normolipid diet group with IF (ICT group), the animals showed microbiota composition similar to those found in animals that consumed high-fat diets. These alterations negatively interfered with the production of acetate and butyrate and may influence intestinal homeostasis and compromise metabolism. This study opens important discussions about the physiological impact of IF in health.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0