Bifidobacterium bifidum FL-228.1 enhances mucus barrier via succinic acid-mediated PI3K-Akt/MUC2 signaling pathway in vitro

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-09-23 DOI:10.1016/j.fbio.2025.107645

Guoyan Wang , Jiajia Ye , Wenjing Du , Yang Li , Yongping Wang , Huaxi Yi , Lanwei Zhang , Tongjie Liu

{"title":"Bifidobacterium bifidum FL-228.1 enhances mucus barrier via succinic acid-mediated PI3K-Akt/MUC2 signaling pathway in vitro","authors":"Guoyan Wang , Jiajia Ye , Wenjing Du , Yang Li , Yongping Wang , Huaxi Yi , Lanwei Zhang , Tongjie Liu","doi":"10.1016/j.fbio.2025.107645","DOIUrl":null,"url":null,"abstract":"<div><div><em>Bifidobacterium bifidum</em> is widely recognized for its beneficial effects on gut health. Our previous work identified <em>B. bifidum</em> FL-228.1, a strain isolated from infant feces, as particularly effective in protecting intestinal barrier function; however, the underlying mechanisms remained unclear. In this study, treatment with <em>B. bifidum</em> FL-228.1 significantly enhanced MUC2 expression in LS174T cells. Non-targeted metabolomic profiling revealed a marked elevation of succinic acid among the metabolites of <em>B. bifidum</em> FL-228.1. Exogenous supplementation with succinic acid similarly promoted MUC2 expression in LS174T cells, and transcriptomic analysis implicated the PI3K-Akt pathway in this process. Moreover, inhibition of PI3K with LY294002 suppressed succinic acid-induced MUC2 upregulation, confirming the essential role the pathway. Collectively, these findings elucidate a mechanism by which <em>B. bifidum</em> FL-228.1 enhances MUC2 expression, providing a theoretical foundation for its potential application in improving intestinal barrier function.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107645"},"PeriodicalIF":5.9000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Bioscience","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221242922501822X","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Bifidobacterium bifidum is widely recognized for its beneficial effects on gut health. Our previous work identified B. bifidum FL-228.1, a strain isolated from infant feces, as particularly effective in protecting intestinal barrier function; however, the underlying mechanisms remained unclear. In this study, treatment with B. bifidum FL-228.1 significantly enhanced MUC2 expression in LS174T cells. Non-targeted metabolomic profiling revealed a marked elevation of succinic acid among the metabolites of B. bifidum FL-228.1. Exogenous supplementation with succinic acid similarly promoted MUC2 expression in LS174T cells, and transcriptomic analysis implicated the PI3K-Akt pathway in this process. Moreover, inhibition of PI3K with LY294002 suppressed succinic acid-induced MUC2 upregulation, confirming the essential role the pathway. Collectively, these findings elucidate a mechanism by which B. bifidum FL-228.1 enhances MUC2 expression, providing a theoretical foundation for its potential application in improving intestinal barrier function.

查看原文本刊更多论文

两歧双歧杆菌FL-228.1通过琥珀酸介导的PI3K-Akt/MUC2信号通路增强体外粘液屏障

两歧双歧杆菌因其对肠道健康的有益作用而被广泛认可。我们之前的工作发现两歧双歧杆菌FL-228.1，一种从婴儿粪便中分离出来的菌株，在保护肠道屏障功能方面特别有效；然而，潜在的机制仍不清楚。在本研究中，两歧双歧杆菌FL-228.1显著提高了LS174T细胞MUC2的表达。非靶向代谢组学分析显示，两歧双歧杆菌FL-228.1的代谢产物中琥珀酸显著升高。外源补充琥珀酸同样促进了LS174T细胞MUC2的表达，转录组学分析提示PI3K-Akt通路参与了这一过程。此外，LY294002抑制PI3K抑制琥珀酸诱导的MUC2上调，证实了该途径的重要作用。综上所述，这些发现阐明了双歧杆菌FL-228.1增强MUC2表达的机制，为其在改善肠道屏障功能方面的潜在应用提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.