Food Bioscience最新文献

{"title":"Self-nanoemulsifying delivery system of PEITC ameliorates DSS-induced ulcerative colitis by regulating NF-κB pathway, modulating oxidative stress, and barrier repair","authors":"Haibo Huang ,&nbsp;Changlong Ouyang ,&nbsp;Haoxi Ma ,&nbsp;Ping Tang ,&nbsp;Zijun Wang ,&nbsp;Yiyang Hou ,&nbsp;Lijuan Zhu ,&nbsp;Xiaojing Ma","doi":"10.1016/j.fbio.2025.107649","DOIUrl":"10.1016/j.fbio.2025.107649","url":null,"abstract":"<div><div>Ulcerative colitis (UC) is a chronic inflammatory bowel disease that significantly affects patients' quality of life. Although Phenethyl isothiocyanate (PEITC) has shown considerable promise in alleviating intestinal inflammation, the low bioavailability and water insolubility have hindered further development and clinical application. In this study, a self-assembled nanoemulsion (PNE) was successfully developed, and its physicochemical properties and therapeutic efficacy against UC were systematically evaluated. The prepared nanoemulsions displayed excellent stability, anti-inflammatory, and antioxidant activities. In dextran sulfate sodium (DSS)-induced colitis in mice, treatment with PNE significantly alleviated weight loss, restored colon length, reduced DAI score, and mitigated histopathological damage. These therapeutic effects may be attributed to PEITC's ability to attenuate intestinal oxidative stress, suppress inflammatory responses, and enhance the integrity of the intestinal barrier. In conclusion, the results of this study indicate that PNE holds therapeutic potential in the treatment of UC and lays the groundwork for future clinical applications.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107649"},"PeriodicalIF":5.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential antimutagenic activity of foods from Mediterranean diet using the Ames test","authors":"Carlotta Alias,&nbsp;Claudia Zani,&nbsp;Ilaria Zerbini,&nbsp;Donatella Feretti","doi":"10.1016/j.fbio.2025.107642","DOIUrl":"10.1016/j.fbio.2025.107642","url":null,"abstract":"<div><div>Many compounds of foods belonging to the Mediterranean diet were studied for their cardiovascular-protective and anticancer effects, as well as for antimutagenic activities. Among them, fermented foods as yoghurts and vegetables rich in antioxidants were frequently tested. However, the large majority of the studies have been carried out on single functional element of the food (such as bacterial strain or extracted compound).</div><div>Differently, this study aimed to investigate whether a panel of commercially relevant foods belonging to Mediterranean diet (namely, yoghurt, apple, lemon, peach, kiwi, broccoli, onion, tomato) can counteract the effects on DNA of mutagens (2-nitrofluorene, sodium azide, 2-aminofluorene) by using the Ames test with pre-incubation on TA98 and TA100 strains of <em>Salmonella typhimurium.</em></div><div>The greatest inhibitory effect of the mutagens was achieved by yoghurt, followed, in decreasing order, by lemon = kiwi &gt; peach = broccoli &gt; onion = tomato &gt; apple &gt; tomato purée.</div><div>The present findings provide a foundation for further research on the potential DNA protective activity of fermented foods and vegetables.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107642"},"PeriodicalIF":5.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorisation of table olive discards: bioactive composition and bioaccessibility of a novel functional ingredient","authors":"Patricia Rus-Fernández,&nbsp;Miguel Henares,&nbsp;Ana Fuentes","doi":"10.1016/j.fbio.2025.107641","DOIUrl":"10.1016/j.fbio.2025.107641","url":null,"abstract":"<div><div>The table olive production industry generates considerable volumes of by-products every year, mainly olives that do not meet established quality standards and are withdrawn from commercial channels despite having nutritionally desirable constituents. This work aims to characterise the fatty acid profile and bioactive compounds of new ingredients obtained from table olive discards, namely table olive powders, and to determine their antioxidant properties and the bioaccessibility of their phenolic fraction. The results reveal that the stuffing ingredients and the drying process do not affect the obtained product's fatty acid profile, and all the samples are high in oleic (68.25 %–72.43 %), palmitic (16.34 %–18.42 %) and linoleic (4.42 %–6.95 %) acids. Hot-air drying treatment affects samples' contents of phenolic compounds and samples' antioxidant properties, which makes freeze-drying (FD) extremely suitable for obtaining high-antioxidant powder. Nevertheless, the total phenol content of all table olive powders (TOPs) evaluated was high, with values ranging from 5933.3 to 8141.9 mg GAE/kg of dry weight (DW). After <em>in vitro</em> digestion, hydroxytyrosol (HT), recognised as health-promoting agent, presents the highest bioaccessibility, with values exceeding 100 %. This finding suggests that the digestive process may release antioxidant compounds from their precursor forms, thereby enhancing the functionality of TOPs. This research has shown that table olive discards can be a valuable source of bioactive compounds, exhibiting considerable potential for substituting conventional chemical additives, such as antioxidants, and for the innovation of novel ingredients in new formulations intended for human consumption.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107641"},"PeriodicalIF":5.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cinnamaldehyde nanoemulsion disrupts Listeria monocytogenes biofilms: Metabolomics reveals dual inhibition and removal mechanisms","authors":"Yurong Yang ,&nbsp;Huayan Cheng ,&nbsp;Qiujin Zhu ,&nbsp;Jing Wan","doi":"10.1016/j.fbio.2025.107634","DOIUrl":"10.1016/j.fbio.2025.107634","url":null,"abstract":"<div><div>To address the persistent colonization of <em>Listeria monocytogenes</em> (<em>L. monocytogenes</em>) biofilms on food processing surfaces, which poses critical food safety risks, this study investigated the multi-target antibiofilm mechanism of cinnamaldehyde nanoemulsion (CAN) based on its previously established efficacy. Results demonstrated that CAN compromised membrane integrity, increasing intracellular conductivity while facilitating protein and nucleic acid leakage. It concurrently suppressed adenosine triphosphate (ATP) synthesis and accelerated reactive oxygen species (ROS) accumulation, ultimately triggering ion imbalance and metabolic homeostasis collapse. Furthermore, CAN significantly reduced <em>L. monocytogenes</em> motility, enhanced surface hydrophobicity, and inhibited extracellular polymeric substance (EPS) secretion, thereby disrupting biofilm assembly. The sharp decline in viable biofilm cells critically impaired structural recovery. Metabolomics further revealed that CAN inhibited initial adhesion through targeted suppression of fatty acid metabolism and teichoic acid biosynthesis while disrupting biofilm maintenance and repair via interference with ABC transporter functionality and D-amino acid metabolism. This study elucidated the mechanism by which CAN inhibited and removed biofilms from a metabolomics perspective, providing novel insights for biofilm control strategies in the food industry.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107634"},"PeriodicalIF":5.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nisin-mediated organic-inorganic hybrid for the inhibition of Alicyclobacillus acidoterrestris in apple juice","authors":"Hang Jia ,&nbsp;Wenhao Zhang ,&nbsp;Mengyao Wang ,&nbsp;Yanli Xie ,&nbsp;Renyong Zhao ,&nbsp;Tianli Yue","doi":"10.1016/j.fbio.2025.107638","DOIUrl":"10.1016/j.fbio.2025.107638","url":null,"abstract":"<div><div>Juice spoilage caused by microbial contamination has occurred worldwide, among these microorganisms, <em>Alicyclobacillus</em> spp. have received widespread attention owing to the thermos-acidophilic ability. To develop new non-thermal method, antibacterial peptide-mediated organic-inorganic hybrid nanoflowers were designed to control <em>Alicyclobacillus acidoterrestris</em> in this work. Investigation on antibacterial ability demonstrated that the constructed Nisin@NFs damaged bacterial membrane and caused the leakage of intracellular substance. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against <em>A. acidoterrestris</em> were 4.0 μg mL⁻¹ and 32.0 μg mL⁻¹, respectively. Besides, investigation on <em>in vitro</em> hemolysis assay and <em>in vivo</em> zebrafish toxicity evaluation implied that the synthesized nanoflowers displayed negligible adverse effects on biocompatibility. Further application researches indicated that Nisin@NFs possessed effective antibacterial ability against <em>A. acidoterrestris</em> in apple juice without affecting the juice quality. The construction of peptide-mediated organic-inorganic hybrid nanoflowers provided a new idea for the control of <em>A. acidoterrestris</em> contamination in the juice industry.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107638"},"PeriodicalIF":5.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented Sargassum hemiphyllum soluble dietary fiber exhibits enhanced bioactivity and gut microbiota regulation potential in C57BL/6 mice","authors":"Hongying Cai ,&nbsp;Zhuo Wang ,&nbsp;Rui Li ,&nbsp;Qiaoli Zhao ,&nbsp;Bingbing Song ,&nbsp;Xiaofei Liu ,&nbsp;Riming Huang ,&nbsp;Baojun Xu ,&nbsp;Shouchun Liu ,&nbsp;Kit-Leong Cheong ,&nbsp;Saiyi Zhong","doi":"10.1016/j.fbio.2025.107563","DOIUrl":"10.1016/j.fbio.2025.107563","url":null,"abstract":"<div><div>Sargassum, a sustainable marine resource in China, has less explored soluble dietary fiber (SDF) functions and gut microbiota effects. In this study, SDF was extracted through fermentation with three lactic acid bacteria (LAB) strains, <em>Lactobacillus brevis</em>, <em>Bifidobacterium longum</em>, and <em>Streptococcus thermophilus.</em> Fermentation significantly increased SDF yield to 0.22–0.25 g/g, reduced molecular weight to 27.5–54.8 kDa, and enhanced its water-holding capacity and sodium cholate adsorption capacity. Among the three LAB strains, <em>B. longum</em> (BL-SDF) demonstrated the greatest antioxidant improvement, increasing total phenolic content by 42.8 % and reducing IC50 value for DPPH and hydroxyl radicals by 42.36 % and 48.54 %, respectively (<em>p</em> &lt; 0.05). <em>In vivo</em> studies in C57BL/6 mice using 16S rRNA sequencing revealed that BL-SDF significantly enriched the abundance of beneficial species such as <em>Blautia</em>, <em>Roseburia</em>, <em>Parabacteroides</em>, <em>Alistipes</em>, and <em>Muribaculum</em>. These results provide novel evidence that SDF from <em>Sargassum</em>, especially BL-SDF, could be used as a functional ingredient to improve antioxidant properties and promote gut health.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107563"},"PeriodicalIF":5.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Killer yeast in winemaking: A comprehensive review","authors":"Fatima El Dana ,&nbsp;Salem Hayar ,&nbsp;Hervé Alexandre","doi":"10.1016/j.fbio.2025.107631","DOIUrl":"10.1016/j.fbio.2025.107631","url":null,"abstract":"<div><div>The killer phenomenon refers to the ability of certain yeasts to produce toxins that are lethal to sensitive yeast strains and filamentous fungi. Killer toxins exert antimicrobial activity among other things, wine spoilage microorganisms. For this reason, the role of yeast killer toxins in winemaking has gained considerable attention due to their potential to control spoilage organisms and enhance the quality of wine. Killer toxins are proteinaceous compounds produced by certain yeast strains, notably <em>Saccharomyces cerevisiae</em> and various non-<em>Saccharomyces</em> yeasts, which exhibit antimicrobial properties against competing yeast species. However, the production of killer toxins is species and strain dependent. Moreover, this production is modulated by environmental factors, which makes it difficult to exploit this property in an oenological context. Furthermore, studies on killer toxins are often carried out based on agar test results or using purified toxin, which makes it difficult to extrapolate these activities to wine conditions. There are also relatively few studies on killer activity in real ecological contexts and some studies are contradictory. This review will not present the mechanisms of action of killer proteins which have been widely described in the literature [1–9]. We propose a focus on killer toxins in oenological conditions to synthesize knowledge on the role of the killer phenomenon in microbial and fermentation dynamics in wine. But also, to understand their role in the optimization of fermentations, in bioprotection, and in yeast interaction phenomena. The purpose of this review is also to highlight the contradictions and their origins and the scientific gaps relating to the study of this phenomenon in oenological conditions.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107631"},"PeriodicalIF":5.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure-guided engineering of L-aspartate-α-decarboxylase for enhanced substrate tolerance and β-alanine yield","authors":"Lu Xu,&nbsp;Junwen Yuan,&nbsp;Daobin Wang,&nbsp;Ruisi Wu,&nbsp;Jidong Liu,&nbsp;Xiyao Cheng,&nbsp;Xinquan Liang,&nbsp;Ning Li","doi":"10.1016/j.fbio.2025.107633","DOIUrl":"10.1016/j.fbio.2025.107633","url":null,"abstract":"<div><div>β-Alanine is an important precursor for food additives, drugs, and nitrogen-containing compounds, and its biosynthesis is mainly catalyzed by L-aspartate-α-decarboxylase (PanD). However, the inherent limitations of wild-type PanD, including low catalytic efficiency and pronounced substrate inhibition at high concentrations, significantly constrain its industrial applications. This study systematically compared three PanD orthologs (EcPanD from <em>Escherichia coli</em>, CgPanD from <em>Corynebacterium glutamicum</em>, and BsPanD from <em>Bacillus subtilis</em>), revealing BsPanD's superior catalytic efficiency. BsPanD exhibited substrate inhibition at high L-aspartate concentrations, with conversion rates of 78 % and 52 % at 60 g/L and 100 g/L substrate, respectively. Through the construction of protein structures and reasonable structure-oriented design, as well as computational screening and experimental verification, we selected five mutants (BsPanD^T4W, BsPanD^I33A, BsPanD^P40N, BsPanD^I88W, BsPanD^N109E) with significantly improved tolerance from 40 candidate mutants. These mutants achieved 90.6–92.9 % conversion rates at 60 g/L substrate, yielding 36.4–37.4 g/L β-alanine. In addition, kinetic analyses demonstrated reduced K_m values (28–45 % decrease) in all mutants, indicating enhanced substrate affinity. Notably, BsPanD^I33A exhibited the highest catalytic efficiency (<em>k</em>_<em>cat</em>/K_m = 1.95 s⁻¹ mmol⁻¹ L, a 57.3 % increase over the wild-type), attributed to reduced steric hindrance from the Ile33Ala mutation. With its exceptional performance at high substrate concentration (92.9 % conversion at 60 g/L) and optimized catalytic efficiency, BsPanD^I33A emerges as the most promising biocatalyst for industrial β-alanine production. These results provide novel insights into PanD catalysis and establish a robust platform for industrial β-alanine biosynthesis.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107633"},"PeriodicalIF":5.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apios americana Medikus tuber starch alleviates diphenoxylate-induced constipation by modulating neurotransmitters and gut microbiota in mice","authors":"Tao Li ,&nbsp;Min Jiang ,&nbsp;Wenhua Ji ,&nbsp;Pengjie Han ,&nbsp;Jingguo Xu ,&nbsp;Hong Liu ,&nbsp;Xiao Wang","doi":"10.1016/j.fbio.2025.107635","DOIUrl":"10.1016/j.fbio.2025.107635","url":null,"abstract":"<div><div>As the modulator of gut microbiota, resistant starch (RS) demonstrates the significant therapeutic efficacy against difficult defecation. This study investigated the novel prebiotic effects of <em>Apios americana</em> Medikus tuber starch (AS) on diphenoxylate-induced constipation in mice. AS was characterized by a typical B-type crystalline pattern with a high RS content of 87.25 %. Treatment with the high dosage of AS (AS-H), the wet weight and number of black feces, and gastrointestinal transit ratio of constipated mice significantly enhanced by 57.5 %, 242.9 % and 33.9 %, respectively, in comparison with the model group. Moreover, AS-H supplementation significantly inhibited the levels of inflammation-related factors including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) as well as the increased protein expression levels of zonula occluden-1 (ZO-1) and occludin in the colon tissue of constipated mice. AS-H group showed marked increases in the excitatory neurotransmitters with concurrent decreases in the inhibitory neurotransmitters. Additionally, the colonic expression of vasoactive intestinal peptide receptor 1 (VIPR1) was significantly suppressed, while serotonin receptor 4 (5-HT4) was enhanced by AS-H treatment. A significant increase at gut bacterial genus level (such as <em>Bacteroides</em>, <em>Lachnospiraceae_NK4A136_group</em> and <em>Oscillibacter</em>) accompanied by increasing concentrations of SCFAs was observed after AS-H treatment, with these changes directly correlating to the alleviation of constipation. Importantly, fecal microbiota transplantation (FMT) intervention with a AS-derived microbiome notably ameliorated the fecal parameters in constipated mice. Collectively, supplementation with AS could promote the fecal excretion by modulating enteric neurotransmitters and gut microbiota in mice, providing the conduct of subsequent human clinical trials.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107635"},"PeriodicalIF":5.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediococcus acidilactici CCFM1344 intervention during pregnancy prevents maternal high-fat diet-induced autism-like behaviors in early-life offspring mice","authors":"Jingge Sun ,&nbsp;Qing Li ,&nbsp;Xin Qian ,&nbsp;Jingyu Wang ,&nbsp;Zhiying Jin ,&nbsp;Jianxin Zhao ,&nbsp;Wei Chen ,&nbsp;Peijun Tian","doi":"10.1016/j.fbio.2025.107636","DOIUrl":"10.1016/j.fbio.2025.107636","url":null,"abstract":"<div><div>Maternal health plays a crucial role in fetal neurodevelopment, with high-fat diet (HFD) consumption before or during pregnancy known to induce neurodevelopmental and behavioral abnormalities in offspring. In this study, we assessed whether supplementation with <em>Pediococcus acidilactici</em> CCFM1344 (10⁹ CFU/day, oral gavage) during pregnancy in C57BL/6J mice could serve as an effective strategy to counteract neurodevelopmental impairments in offspring induced by maternal HFD. Female mice (8 weeks old) were used for breeding, and both male and female offspring were included in the analyses. 16S rRNA gene sequencing revealed that CCFM1344 significantly modulated the maternal gut microbiota composition, reduced the Firmicutes-to-Bacteroidetes (F/B) ratio, and enriched beneficial genera <em>Alloprevotella</em> and <em>Rikenellaceae RC9 gut group</em>. Behavioral assessments, including the open field and three-chamber social interaction tests, demonstrated that CCFM1344 effectively ameliorated hyperactivity and social deficits in juvenile offspring, with these behavioral changes showing partial normalization in adulthood. Gut microbiota analysis of the juvenile offspring indicated that CCFM1344 significantly affected microbial diversity and improved ecological stability. In mothers, fecal metabolomics and serum cytokine profiling showed that CCFM1344 intervention regulated key metabolites, including taurine and L-histidine, and modulated inflammatory markers such as TNF-α, IFN-γ, and IL-10. In addition, transcriptomic and quantitative real-time PCR analyses of neonatal brain tissue revealed that CCFM1344 reversed the abnormal expression of genes associated with neuroinflammation and myelination—including <em>Gfap</em>, <em>Mmp9</em>, <em>Il-1β</em>, and <em>Mobp</em>—with these changes normalizing by adulthood. In conclusion, pregnancy-specific administration of CCFM1344 modulated the maternal gut microbiota and immunometabolic environment, alleviating HFD-induced impairments in early brain development and behavior in offspring. These findings highlight the critical importance of probiotic interventions during pregnancy in supporting neurodevelopmental health in the next generation.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"73 ","pages":"Article 107636"},"PeriodicalIF":5.9,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}