Food Bioscience最新文献

{"title":"Microgreens: Cultivation practices, bioactive potential, health benefits, and opportunities for its utilization as value-added food","authors":"Mahendra Gunjal ,&nbsp;Jyoti Singh ,&nbsp;Jaspreet Kaur ,&nbsp;Sawinder Kaur ,&nbsp;Vikas Nanda ,&nbsp;Ankita Sharma ,&nbsp;Prasad Rasane","doi":"10.1016/j.fbio.2024.105133","DOIUrl":"10.1016/j.fbio.2024.105133","url":null,"abstract":"<div><div>In recent years, changes in diet patterns and preferences for fresh food commodities, ready-to-eat food products, functional foods, and nutraceuticals have increased. Microgreens (young vegetable greens) are a relatively new form of product that has gained popularity and is also referred to as ‘vegetable confetti’. It is used to improve the sensorial characteristics, <em>viz</em>. appearance and flavor of foods such as salads and main dishes. Compared with seeds and their mature counterparts, microgreens contain greater levels of functional nutrients (minerals, vitamins, antioxidants, and phenolic compounds). This comprehensive review briefly describes the different families of microgreens used for the cultivation of microscale products and highlights their health-promoting bioactive compounds, such as antioxidants, phenolics, pigments, minerals, and vitamins, which are critically associated with the sustainable developmental goals of good health and welfare. In addition to these important factors affecting the cultivation of microgreens, such as their species, type, growth medium, use of nutrients, biofortification, and use of advanced illumination systems, preharvest and postharvest factors affecting microgreens are also addressed.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105133"},"PeriodicalIF":4.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"D-serine alleviates colitis by regulating intestinal α1,2-fucosylation","authors":"Ye Yao ,&nbsp;Xubin Pan ,&nbsp;Yuanyuan Dai ,&nbsp;Yinghan Chen ,&nbsp;Zepeng Chang ,&nbsp;Zhangming Pei ,&nbsp;Yue Xiao ,&nbsp;Hongchao Wang ,&nbsp;Wenwei Lu ,&nbsp;Jianxin Zhao","doi":"10.1016/j.fbio.2024.105057","DOIUrl":"10.1016/j.fbio.2024.105057","url":null,"abstract":"<div><div>Colitis is often accompanied with reduced intestinal α1,2-fucosylation. D-serine has been reported to prevent chronic colitis and upregulate the α1,2-fucosylation levels of intestinal epithelial cells <em>in vitro.</em> However, the role of D-serine in acute colitis and whether α1,2-fucosylation regulation is involved in the process remains unclear. In this study, D-serine alleviated body weight loss, colon shortening, and intestinal barrier damage in mice with acute colitis. Additionally, D-serine helped maintain gut microbiota balance by increasing the abundance of beneficial bacteria, including <em>Bifidobacterium</em>, and decreasing the harmful bacteria, such as <em>Escherichia. Shigella</em>. Furthermore, untargeted metabolomics showed that D-serine can modify the metabolism of cecal microbiota by decreasing concentrations of colitis-associated metabolites. Nevertheless, inhibiting α1,2-fucosylation impaired D-serine-mediated alleviation of colitis, highlighting the importance of α1,2-fucosylation upregulation in this process. D-serine significantly increased the trans-epithelial resistance of normal colonic epithelial cells, which was impaired by α1,2-fucosylation inhibition. Additionally, D-serine enhanced α1,2-fucosylation of macrophages (RAW264.7 cells) and reduced the secretion of tumor necrosis factor-α. The higher expression of the serine uptake gene <em>Slc3a5</em> in type 3 innate lymphoid cells (ILC3s) suggested that D-serine may regulate intestinal α1,2-fucosylation by affecting IL-22 secretion of ILC3s. Taken together, our study showed that D-serine alleviates acute colitis by regulating α1,2-fucosylation of intestinal epithelial cells and macrophages. These findings suggest that regulating intestinal α1,2-fucosylation could be a potential strategy for the treatment of colitis.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105057"},"PeriodicalIF":4.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, identification and screening of anti-osteoporosis milk-derived peptides: Intervention effects in osteoporosis rats","authors":"Yingrui Zhang ,&nbsp;Yue Niu ,&nbsp;Caiqi Fu ,&nbsp;Lingjiao Zhu ,&nbsp;Hongxun Wang ,&nbsp;Yang Yi ,&nbsp;Wei Xu ,&nbsp;Danjun Guo","doi":"10.1016/j.fbio.2024.105120","DOIUrl":"10.1016/j.fbio.2024.105120","url":null,"abstract":"<div><div>To identify milk-derived peptides with both antioxidant and calcium absorption activities in combating osteoporosis, we employed a comprehensive screening approach that included virtual enzymatic hydrolysis, molecular docking, and cellular experiments using osteoblasts. Under the optimal conditions for dual-enzyme hydrolysis, the 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical scavenging rate and soluble calcium binding capacity of the milk-derived peptides were 19.69% and 0.6965 μg/mL, respectively. Six peptide segments, namely KEDVPSER, HKEMPFPK, YPSYG, EDVPSE, VPQLE, and IPAVF, were identified through UPLC-Q-Exactive Orbitrap MS and molecular docking for further validation. Among the peptides, YPSYG significantly promoted the proliferation of MC3T3-E1 cells both with and without CaCl₂ (<em>P</em> &lt; 0.05), increasing proliferation by 38.27% and 20.67%, respectively, compared to the control group. Additionally, YPSYG significantly improved proliferation after H₂O₂-induced oxidative damage (<em>P</em> &lt; 0.05), with a 38.23% higher rate than the model group. Compared with rats in the osteoporosis model group, YPSYG significantly enhanced serum alkaline phosphatase (ALP) and N-terminal propeptide of type I procollagen in rats (s-PINP) levels and decreased tartrate-resistant acid phosphatase (TRAP) levels (<em>P</em> &lt; 0.05). Furthermore, milk-derived peptides and YPSYG significantly increased the bone weight index, maximum load, and bending energy of the femur and tibia in osteoporotic rats (<em>P</em> &lt; 0.05). Additionally, these peptides significantly reduced the number of osteoclasts in the metaphysis of the femur and tibia in osteoporotic rats and alleviated microstructural damage. This study confirmed that milk-derived peptides, including YPSYG, effectively promoted bone formation and improved bone microstructure in osteoporotic rats. These findings provided a foundation for developing functional foods for elderly bone health.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105120"},"PeriodicalIF":4.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation with cysteine improved metabolic syndrome in rats by increasing antioxidant potential in the liver and adipose tissue, as well as decreasing hepatic NF-κB expression","authors":"Sina Mahdavifard ,&nbsp;Manouchehr Nakhjavani","doi":"10.1016/j.fbio.2024.105123","DOIUrl":"10.1016/j.fbio.2024.105123","url":null,"abstract":"<div><p>Insulin resistance is a key characteristic of metabolic syndrome (MetS). The hepatic nuclear factor- κB (NF-κB) signaling pathway plays a crucial role in insulin resistance and the development of type 2 diabetes. Our study aimed to examine the impact of cysteine (Cys) on various biochemical and histopathological parameters in the liver and kidney, hepatic NF-kβ expression, oxidative stress, inflammation, glycation, carbonyl stress markers, and insulin resistance. The study involved four groups of rats, each consisting of seven rats: a control group, a MetS group, and two similar groups receiving Cys treatment. Metabolic syndrome was induced in rats by administering a 40% sucrose solution, while, the treated groups received 50 mg/L Cys in their drinking water. Various factors, including body weight, hepatic NF-kβ expression, levels of antioxidants, <em>anti</em>-glycation, oxidative stress, carbonyl stress, inflammatory, <em>anti</em>-glycation, and glycation markers were assessed in blood and tissues. Liver and kidney function parameters and metabolic profiles were measured. Finally, liver tissue was also evaluated by a pathologist. The results showed that Cys reduced hepatic NF-kβ expression, oxidative stress, inflammation, glycation and carbonyl stress markers, as well as liver fatty content, blood sugar levels, insulin resistance, cardiovascular risk index, and body weight. The treatment also mitigated histopathological liver changes and acute hepatitis <em>(p &lt; 0.001).</em> Cysteine exhibited anti-obesity and anti-atherosclerotic effects, improved β-cell function, insulin sensitivity, and lipid metabolism, and enhanced liver and kidney function, as well as prevented acute hepatitis by restoring the GSH/GSSG ratio, hepatic NF-kβ signaling, and carbonyl stress.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105123"},"PeriodicalIF":4.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary oxygenated water mitigates type 2 diabetes mellitus by modulating gut microbiota and enhancing glucose metabolism in skeletal muscle","authors":"Yue Xiao ,&nbsp;Dongmei Yang ,&nbsp;Xiaoqing Chen ,&nbsp;Li Xiong ,&nbsp;Huan Guo ,&nbsp;Sheng Ma ,&nbsp;Bin Li ,&nbsp;Hong Gao ,&nbsp;Yina Huang","doi":"10.1016/j.fbio.2024.105110","DOIUrl":"10.1016/j.fbio.2024.105110","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) is a significant health concern resulting from dysregulation of glucose metabolism, emphasizing the need for effective dietary interventions. Emerging evidence suggests a correlation between diabetes and hypoxia, indicating that oxygenated water (ORW) may hold promise in mitigating T2DM. This study employed a multifaceted approach involving physiological, genomic, metabolomic, transcriptomic, and molecular biological analyses to investigate the effects of ORW on T2DM and its underlying mechanisms. The results demonstrated that ORW effectively ameliorated key symptoms of T2DM by improving polydipsia and polyphagia, reducing fasting blood glucose, enhancing glucose tolerance, and increasing insulin levels. Notably, the beneficial effects of ORW appeared to be mediated through modulation of gut-muscle interactions by influencing gut microbiota composition and metabolite profiles. Transcriptomic and molecular analyses revealed that ORW activated the Akt signaling pathway and upregulated key genes involved in glucose uptake and utilization in skeletal muscle, as evidenced by increased p-AKT/AKT ratio and upregulation of <em>Irs1</em>, <em>Glut4</em>, <em>Hk2</em>, and <em>Pfk</em>. Collectively, these findings demonstrate that ORW alleviates T2DM by modulating gut microbiota while enhancing glucose uptake/utilization in skeletal muscle, supporting its potential use as an effective adjuvant strategy for targeted regulation of T2DM.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105110"},"PeriodicalIF":4.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical compositions and health promoting effects of wild edible mushroom milk-cap (Lactarius deliciosus): A review","authors":"Ziliang Su,&nbsp;Baojun Xu","doi":"10.1016/j.fbio.2024.105118","DOIUrl":"10.1016/j.fbio.2024.105118","url":null,"abstract":"<div><p><em>Lactarius deliciosus</em> is a kind of nutritious edible and medical wild mushroom, belonging to <em>Lactarius</em> genus and Russualaceae family. It has a symbiotic relationship with the roots of the host plant, forming symbiotic bodies and producing fruiting bodies. Due to the potential value for medicinal use, the health effects of <em>L. deliciosus</em> have been studied widely. In this paper, chemical compositions of <em>L. deliciosus</em> and the health effects related to the bioactive components are summarized. The literatures in this article are from ScienceDirect, Jane, Bing Academic, CNKI, ResearchGate, and PubMed. It is rich in protein, minerals, carbohydrates and taste compounds with lower calories and fat content. In addition, it had been confirmed that <em>L. deliciosus</em> have health effects such as antihyperglycemic, immune regulatory, and anti-tumor activity. For its future application and development, its molecular mechanisms and clinical trials in the field of immunity still need to be explored.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105118"},"PeriodicalIF":4.8,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic compounds in dietary target the regulation of gut microbiota: Role in health and disease","authors":"Qinqiu Zhang ,&nbsp;Yi Xu ,&nbsp;Danka Bukvicki ,&nbsp;Yue Peng ,&nbsp;Fan Li ,&nbsp;Qing Zhang ,&nbsp;Jing Yan ,&nbsp;Shang Lin ,&nbsp;Shuxiang Liu ,&nbsp;Wen Qin","doi":"10.1016/j.fbio.2024.105107","DOIUrl":"10.1016/j.fbio.2024.105107","url":null,"abstract":"<div><div>Mechanisms of interaction between phenolic compounds (PCs) and gut microbiota have received widespread attention. Excluding individual differences in absorption and metabolism, the bioavailability and stability of PCs depend on the composition of the gut microbiota and the short-chain fatty acid content, producing a series of enzymatic hydrolytic, splitting and reduction reactions. Meanwhile, PCs can be used as prebiotics, and alter the <em>Firmicutes</em>/<em>Bacteroidetes</em>, reduce harmful bacteria and enrich short-chain fatty acids (SCFAs)-producing bacteria, thereby protecting and regulating the intestinal barrier and immune system. In various diseases, such as metabolic disease, cardiovascular diseases, neurological diseases and cancer, PCs can reduce clinical symptoms and reverse the disease through different pathways, such as facilitating metabolic inflammation mainly through the Lipopolysaccharide (LPS)/Toll-like receptor 4/Nuclear Factor Kappa B signaling pathway. SCFAs and LPS can be also used as two major biomarkers for the interaction PCs and gut microbiota. Based on recent findings, a comprehensive review of PCs biotransformation and gut microbiota interaction mechanisms is summarized. It will provide some perspective on selecting and consuming PCs with probiotic activity.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105107"},"PeriodicalIF":4.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viola stocksii: A rich source of antioxidant and anti-inflammatory flavonoid glycosides with converged therapeutic potential against SARS-CoV-2 MPro, spike trimer, and surface glycoproteins","authors":"Roshan Zamir ,&nbsp;Shanza Munir ,&nbsp;Humaira Yasmeen Gondal ,&nbsp;Muhammad Nisar ,&nbsp;Sami A. Al-Hussain ,&nbsp;Ahmed Abbaskhan ,&nbsp;Zain M. Cheema ,&nbsp;Magdi E.A. Zaki ,&nbsp;M. Iqbal Choudhary","doi":"10.1016/j.fbio.2024.105095","DOIUrl":"10.1016/j.fbio.2024.105095","url":null,"abstract":"<div><p>This research presents the first comprehensive investigation into the phytochemical and nutraceutical properties of <em>Viola stocksii.</em> The study aimed to identify the therapeutic efficacy and bioactive constituents of this medicinal herb, traditionally used as an herbal tea for managing respiratory and abdominal issues in remote areas of Pakistan. The therapeutic efficacy of the plant was assessed as an anti-oxidant-rich health tonic coupled with anti-inflammatory and immunomodulatory activities against COVID-19 and its evolving variants. The study involved sequential extraction, bio-assay guided fractionation, isolation, characterization, and biological evaluation. Preliminary <em>in vitro</em> studies have demonstrated that polar fractions of the plant possess significant antioxidant and antiinflammatory potential. A diverse combination of chromatographic techniques (Diaion resin HP-20, Sephadex LH-20, ODS-C18, and RP-HPLC) facilitated the purification of active constituents of the plant. Comprehensive analysis of NMR (¹H, ¹³C, and 2D NMR) and mass (EIMS, HR-EIMS, and FAB-HRMS) spectral data lead to characterize the isolated compounds (<strong>1</strong>–<strong>4</strong>) as flavonoid glycosides. Results from <em>in vitro</em> and <em>in-silico</em> evaluations revealed that the isolated glycosides exhibit high antioxidant, antiinflammatory, and immunomodulatory activities, which synergistically show promising potential in combating SARS-CoV-2 variants. Besides M^Pro, therapeutic potential against two unexplored proteins i.e. the spike trimer (7WZ1) and surface glycoproteins (7QTJ) was studied for the first time. Kaempferol (<strong>KamGluαRh</strong>) and quercetin (<strong>QurGluαRh</strong>) carrying rutinoside, were identified as the most active constituents of the plant, with the highest binding affinities in the range of −10 to −13 kcal/mol. Ten distinct active regions were explored on the spike trimer, including strong binding affinity with the essential RBD region, spike head, and tail of the protein. MD simulation validated the stability of <strong>M</strong>^{<strong>pro</strong>}-<strong>KamGluαRh</strong> and <strong>M</strong>^{<strong>pro</strong>}<strong>-QurGluαRh</strong> complexes evidenced by an average RMSD of 0.2 nm led to the mechanistic understanding of M^pro inhibition. ADMET and pharmacokinetic studies authenticated the therapeutic ability and drug-likeness profiling of all extracted compounds. Results revealed the beneficial role of glycosides in boosting the medicinal effectiveness of flavonoids and the potential of <em>Viola stocksii</em> as an immunity booster against respiratory infections like COVID-19.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105095"},"PeriodicalIF":4.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2212429224015256/pdfft?md5=0677c54d568d48c4bdc8a7af9f9c2dfa&pid=1-s2.0-S2212429224015256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the potential susceptibility of planktonic cells and bacterial biofilms by diosgenin","authors":"Maria do Socorro Costa ,&nbsp;Ana Raquel Pereira da Silva ,&nbsp;Nara Juliana Santos Araújo ,&nbsp;Cícera Laura Roque Paulo ,&nbsp;Gabriel Gonçalves Alencar ,&nbsp;Maria Anesia Sousa de Alencar ,&nbsp;Talysson Felismino Moura ,&nbsp;Clara Mariana Gonçalves Lima ,&nbsp;Thiago Sampaio de Freitas ,&nbsp;José Maria Barbosa Filho ,&nbsp;Jacqueline Cosmo Andrade-Pinheiro ,&nbsp;Henrique Douglas Melo Coutinho","doi":"10.1016/j.fbio.2024.105106","DOIUrl":"10.1016/j.fbio.2024.105106","url":null,"abstract":"<div><p>Bacterial biofilms are formed by a complex community of microorganisms, held together by a matrix that provides structural support and stability to microbial communities, making infections associated with biofilms more difficult to treat. In the face of growing research in the health area in search of antibiotics with action against biofilms, natural products present an effective alternative strategy. In this context, the aim of this study was to evaluate bacterial biofilm formation, assess the susceptibility of planktonic cells to diosgenin, and examine its efficacy on inhibiting and treating biofilms. The broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC). Six bacterial strains were tested: <em>Staphylococcus aureus</em> ATCC 25923, <em>Pseudomonas aeruginosa</em> ATCC 9027, <em>Pseudomonas aeruginosa</em> 24, <em>Enterococcus faecalis</em> INCQS 0017, <em>Enterococcus faecalis</em> INCQS 00018, and <em>Enterococcus faecalis</em> INCQS 654. The crystal violet method was employed to evaluate the potential of diosgenin in biofilm inhibition and eradication. Chlorhexidine gluconate served as a reference standard for the antibiofilm assays, along with the antibiotics norfloxacin, ampicillin, and gentamicin. MIC determination revealed that diosgenin exhibited intrinsic antimicrobial activity, with an MIC of 406 μg/mL against <em>S. aureus</em> ATCC 25923. The antibiofilm activity showed significant inhibition for <em>E. faecalis</em> 17 and moderate inhibition against <em>E. faecalis</em> 654 and <em>S. aureus</em> 25923. Furthermore, diosgenin effectively eradicated preformed biofilms of <em>S. aureus</em> 25923 and <em>P. aeruginosa</em> 9027, indicating its ability to interfere with biofilm biomass. These results suggest that diosgenin not only inhibits bacterial biofilm formation but also eradicates established biofilms, highlighting it as a promising candidate for the development of products aimed at combating bacterial biofilms.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105106"},"PeriodicalIF":4.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Bacillus strains and growth medium in shaping γ-glutamyl peptide production","authors":"Qian Li ,&nbsp;Longteng Zhang ,&nbsp;Lene Jespersen ,&nbsp;Dennis Sandris Nielsen ,&nbsp;René Lametsch","doi":"10.1016/j.fbio.2024.105103","DOIUrl":"10.1016/j.fbio.2024.105103","url":null,"abstract":"<div><p>To compare γ-glutamyl di- and tripeptides generated by different <em>Bacillus</em> strains and investigate the impact of substrates on the production of γ-glutamyl peptides, six strains of four species, including <em>B. subtilis</em>, <em>B. velezensis</em>, <em>B. amyloliquefaciens</em> and <em>B. paralicheniformis</em>, were cultivated in a standard brain heart infusion (BHI) broth and a medium consisting of hemoglobin hydrolysates (HH) for six days. Quantitative analysis of free amino acids and γ-glutamyl peptides, as well as determination of bacterial growth and γ-glutamyltransferase activity were carried out. Results revealed that all <em>Bacillus</em> strains could generate a series of γ-glutamyl dipeptides in both media. Most strains produced higher concentrations of target peptides in the HH medium (up to 83.56 μM), which correlated with higher levels of free amino acids in the medium. Glutathione was detected only in the BHI medium with <em>B. subtilis</em> PRO84, <em>B. velezensis</em> PRO76, <em>B. altitudinis</em> PRO107, and <em>B. paralicheniformis</em> PRO109 (up to 0.61 μM), indicating the glutathione-forming ability of these <em>Bacillus</em> strains. Production of γ-glutamyl peptides was influenced by both the choice of strain and growth medium, with the medium exerting a more pronounced impact than the strain. This study highlights the importance of selecting the appropriate substrate and <em>Bacillus</em> strains to produce desired γ-glutamyl peptides as enhancers of koku, potentially inspiring the conversion of meat side streams into valuable kokumi seasonings.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"62 ","pages":"Article 105103"},"PeriodicalIF":4.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2212429224015335/pdfft?md5=e548cafeb42669c32cbc3faca2d7d9bb&pid=1-s2.0-S2212429224015335-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}