A nisin-mediated organic-inorganic hybrid for the inhibition of Alicyclobacillus acidoterrestris in apple juice

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-09-22 DOI:10.1016/j.fbio.2025.107638

Hang Jia , Wenhao Zhang , Mengyao Wang , Yanli Xie , Renyong Zhao , Tianli Yue

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引用次数: 0

Abstract

Juice spoilage caused by microbial contamination has occurred worldwide, among these microorganisms, Alicyclobacillus spp. have received widespread attention owing to the thermos-acidophilic ability. To develop new non-thermal method, antibacterial peptide-mediated organic-inorganic hybrid nanoflowers were designed to control Alicyclobacillus acidoterrestris in this work. Investigation on antibacterial ability demonstrated that the constructed Nisin@NFs damaged bacterial membrane and caused the leakage of intracellular substance. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against A. acidoterrestris were 4.0 μg mL⁻¹ and 32.0 μg mL⁻¹, respectively. Besides, investigation on in vitro hemolysis assay and in vivo zebrafish toxicity evaluation implied that the synthesized nanoflowers displayed negligible adverse effects on biocompatibility. Further application researches indicated that Nisin@NFs possessed effective antibacterial ability against A. acidoterrestris in apple juice without affecting the juice quality. The construction of peptide-mediated organic-inorganic hybrid nanoflowers provided a new idea for the control of A. acidoterrestris contamination in the juice industry.

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乳清蛋白介导的有机-无机杂合物对苹果汁中嗜酸地酸杆菌的抑制作用

微生物污染引起的果汁腐败在世界范围内都有发生，在这些微生物中，aliicyclobacillus spp.由于其嗜热嗜酸的能力而受到广泛关注。为了开发一种新的非热方法，设计了抗菌肽介导的有机-无机杂交纳米花来控制嗜酸地酸杆菌。抗菌能力的研究表明，构建的Nisin@NFs破坏了细菌膜，造成细胞内物质的渗漏。最小抑菌浓度（MIC）为4.0 μ mL−1，最小杀菌浓度（MBC）为32.0 μ mL−1。此外，体外溶血实验和斑马鱼体内毒性评价表明，合成的纳米花对生物相容性的不良影响可以忽略不计。进一步的应用研究表明，Nisin@NFs对苹果汁中的嗜酸葡萄球菌具有有效的抑菌能力，且不影响果汁品质。多肽介导的有机-无机杂交纳米花的构建为果汁工业中酸地霉污染的防治提供了新的思路。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.