Food Science and Human Wellness最新文献

{"title":"Isoliensinine alleviates cardiac apoptosis by activating the PI3K/AKT Pathway: Insights from <em>in Vivo</em> and <em>in Vitro</em> Studies","authors":"Meizhu Wu, Lingqi Chen, Zhi Guo, Hongshu Liu, Yi Xie, Guosheng Lin, Qiaoyan Zeng, Shilan Chen, Rongji Chen, Yi Huang, Farman Ali, Dawei Lian, Aling Shen, Jun Peng","doi":"10.26599/fshw.2025.9250865","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250865","url":null,"abstract":"This study investigated the cardioprotective effects of Isoliensinine, a bisbenzylisoquinoline alkaloid from <em>Nelumbo nucifera</em> Gaertn, and its underlying mechanisms. Spontaneous hypertensive rats (SHR) received Isoliensinine (2.5, 5, 10 mg/kg/day) or valsartan (10 mg/kg/day) for 10 weeks, with Wistar-Kyoto rats as controls. Cardiac function, histopathology, apoptosis, and transcriptomic changes were evaluated <em>in vivo</em> and in Angtension II (AngII)-stimulated H9c2 cardiomyocytes <em>in vitro</em>. Isoliensinine significantly improved left ventricular function, and ameliorated pathological cardiac damage in SHR. Transcriptomic analysis revealed that Isoliensinine reversed 111 up-regulated and 127 down-regulated transcripts in cardiac tissues of SHR, with enrichment of pathways related to apoptosis and the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. Both <em>in vivo</em> and <em>in vitro</em> experiments confirmed that Isoliensinine reduced cardiomyocyte apoptosis and restored PI3K/AKT phosphorylation. Cellular thermal shift assay (CETSA) and molecular docking supported potential binding of Isoliensinine to PI3K and AKT. Co-treatment with PI3K/AKT activator 740Y-P did not negate the effects of Isoliensinine, suggesting pathway specificity. These findings indicated that Isoliensinine alleviates hypertensive cardiac dysfunction by inhibiting cardiomyocyte apoptosis primarily through modulation of the PI3K/AKT pathway and may serve as a promising therapeutic agent for hypertension.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sandwich-Type SERS Aptasensor Based on Aptamer-functionalized Magnetic Capture Probe and AuNPs@MIL-101 Signal Enhancement Probe for Ultrasensitive Detection of <em>Bifidobacterium bifidum</em>","authors":"Huan Li, Zhuangzhuang Tang, Jian Chen, Qiao Fan, Huiying Xu, Song Miao, Yanbo Wang","doi":"10.26599/fshw.2025.9250890","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250890","url":null,"abstract":"Rapid and on-site detection of <em>Bifidobacterium bifidum </em><em>(</em><em>B. bifidum</em><em>)</em> throughout the food manufacturing process serves as an essential sentinel for assessing product quality and healthy potency. Herein, we introduce a sandwich-type surface-enhanced Raman scattering (SERS) aptasensor for <em>B. bifidum</em> quantification, which integrates aptamer-functionalized magnetic beads (Apt-Fe₃O₄) for target-specific capture and aptamer-modified gold nanoparticles/metal-organic framework composites (Apt-AuNPs@MIL-101) for SERS-based signal amplification. Experimental results revealed a strong linear correlation between the intensity of the characteristic Raman peak and the logarithmic of the <em>B. bifidum</em> concentrations within the range of 2.0 × 10¹ to 2.0 × 10⁶ CFU/mL, and the limit of detection was as low as 2.5 CFU/mL. In addition to superior sensitivity, the proposed approach demonstrated exceptional specificity against non-target bacterial strains, particularly in multi-strain coexistent systems. The method further demonstrated great potential in analysis of <em>B. bifidum</em> in commercial samples, showing high consistency with the results obtained from the classic qPCR method. The study established an innovative analytical platform for rapid and on-site probiotic assessment, highlighting its applicability in probiotic food products’ quality control and evaluation.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250890.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The vascular protective effects of Perillae folium in hyperlipidemia: An integrated strategy integrating metabolomics, network pharmacology and <em>in vitro</em> / <em>in vivo</em> validation","authors":"D. L. Li, Xingyong Zhang, Zhenchang Wang, Ye Liu, Jiayan Qian, Peidong Chen, Zihui Huang, Mingliang Gao, Rui Deng","doi":"10.26599/fshw.2025.9250863","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250863","url":null,"abstract":"Dietary intervention is a reliable approach to protect high-risk individuals against cardiovascular events. Perillae folium (PF) in China is valued for its medicinal, nutritional, and economic benefits, particularly in managing diet-induced lipid metabolism disorders. However, unclear active constituents and mechanisms hinder its quality control and resource utilization. In this study, notable regional variations in the compound profiles of samples sourced from different areas were uncovered in qualitative and quantitative analyses. A comprehensive strategy was implemented, integrating network pharmacology, metabolomics, and experimental validations conducted both <em>in vivo</em> and <em>in vitro</em>, to investigate potential active compounds and mechanisms of action. Four compounds (apigenin-7-<em>O</em>-diglucuronide, shisonin, scutellarin, and rosmarinic acid) were identified as the primary active constituents. PF demonstrated cardiovascular protective effects by modulating the arginine biosynthesis pathway to increase the availability of <em>L</em>-arginine, as well as activating the PI3K-AKT1 signaling pathway, thereby facilitating the release of nitric oxide (NO).","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucoidan attenuated diabetic kidney disease through liver-kidney crosstalk based on CXCL1","authors":"S. Jiang, Li‐Ping Jiang, Chenyu Lu, Yuanyuan Tan, Lisa X. Xu, Tao Yan, Zhaoyi Zhong, Yangting Zhang, Wenyan Yu, Tonghui Zhang, Xin Gao, Qi Yan, Qiuzhen Wang","doi":"10.26599/fshw.2025.9250856","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250856","url":null,"abstract":"As a major microvascular complication of diabetes, the incidence of diabetic kidney disease (DKD) is increasing progressively, especially in the elderly. Early intervention is essential for the prevention and treatment of DKD. Organ crosstalk has a strong association in the progression of DKD, while C-X-C motif chemokine ligand 1 (CXCL1), a chemokine plays a critical role in the liver-kidney axis. Our previous studies found that fucoidan interventions prevented the progression of long-term high-fat diet-induced DKD, while also improving liver damage. However, the precise mechanisms through which fucoidan influences CXCL1 associated with liver-kidney crosstalk in DKD are not yet fully understood. The purpose of this study was to investigate the possible mechanism of fucoidan on the high-fat diet-induced DKD. Sixty male C57BL/6J mice were randomly divided into control, DKD, metformin (MET), low dose fucoidan (LFC, 50 mg/kg/d), middle dose fucoidan (MFC, 100 mg/kg/d), and high dose fucoidan (HFC, 200 mg/kg/d) groups for 24 weeks. Compared to the DKD group, HFC significantly reduced the urine creatinine ((555.06 ± 87.67) μg/L vs. (429.62 ± 33.29) μg/L; <em>P &lt; </em>0.05) and glomerular hypertrophy ((61.46 ± 4.54) mmol/L vs. 54.86±2.66 mmol/L; <em>P &lt; </em>0.05). Moreover, HFC treatment significantly reduced the CXCL1 level in the liver ((37.51 ± 6.70) pg/ng prot vs. (63.64 ± 7.15) pg/ng prot; <em>P &lt; </em>0.05) and kidney ((38.77 ± 8.55) pg/ng prot vs. (56.76 ± 7.50) pg/ng prot; <em>P &lt; </em>0.05) compared to the DKD group. Fucoidan treatment inhibited macrophage recruitment (<em>P &lt; </em>0.05) and modulated the expression of IL-6 and TNF-α in the kidney of DKD mice. Moreover, the expression of cyclic GMP-AMP synthase, stimulator of interferon genes, and nuclear factor kappa-B in the HFC group was significantly lower compared with the DKD group. Collectively, these findings suggest that fucoidan exerts a protective effect against DKD induced by a high-fat diet through the crosstalk between the liver and kidney, based on CXCL1.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250856.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blackcurrant Extract Regulates Lipid Metabolism in <em>Caenorhabditis elegans via</em> SBP-1 and NHR-49","authors":"Liwei Li, Zhe Xu, Rui Liu, Jian Du, Huali Wang, Jianbo Zhang, Ye Zhang, Jianhui Liu, Hao Wang","doi":"10.26599/fshw.2025.9250893","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250893","url":null,"abstract":"Blackcurrant extract (BCE), rich in anthocyanins, has demonstrated significant potential in lipid level reduction. In this study, network pharmacology was applied to predict the lipid-lowering metabolic mechanism of BCE. Upon treatment with BCE (100 μg/mL), lipid accumulation was significantly reduced in both the HepG2 cells in <em>vitro</em> and the high-glucose-fed <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) in<em> vivo</em> by 46.06% and 56.68%. Additionally, the lifespan of <em>C. elegans</em> was significantly prolonged by BCE treatment, its stress tolerance and antioxidant capacity were enhanced, and aging-related markers (e.g., lipofuscin accumulation) were altered. Subsequent qPCR analysis revealed that genes associated with the SBP-1/SREBP pathway (<em>sbp-1</em>,<em> fat-6</em>, and <em>fat-7</em>) were down-regulated by BCE, while genes in the NHR-49/PPARα pathway (<em>nhr-49</em>, <em>acs-2</em>, <em>ech-1.1</em>, and <em>cpt-2</em>) were up-regulated. Furthermore, Oil Red O staining and triglyceride (TG) content assays demonstrated that the hypolipidemic effect of BCE was absent in <em>nhr-49</em>, <em>fat-6</em>, and <em>fat-7</em> mutant strains of <em>C</em><em>. elegans</em>. The current findings were validated by molecular docking, indicating that abnormal fat accumulation in <em>C. elegans</em> was alleviated by BCE treatment via the SBP-1 and NHR-49 pathways. In summary, these findings indicated that BCE exerted a lipid-lowering effect and provided novel insights and research materials for the development of natural lipid-lowering medications and health products.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250893.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cordycepin Attenuates Microglial-Mediated Neurotoxicity in Alzheimer's Disease Through Dual Modulation of Pro-Inflammatory and Phagocytic Functions via Caspase1 Suppression and STAT6 Activation","authors":"Ke Du, Benyu Su, Chunhong Jin, Juan Wang, Luoxuan Su, Xi Zheng, Ying Duan, Mingyan Liu","doi":"10.26599/fshw.2025.9250901","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250901","url":null,"abstract":"Microglia (MG) play dual roles in Alzheimer’s disease (AD) development. They can worsen the condition by causing inflammation but also help in early stages by removing harmful amyloid-beta (Aβ) through phagocytosis. Consequently, precise modulation of microglial phagocytic activity and inflammatory responses holds considerable significance for AD treatment. Cordycepin (Cor) is a representative active component extracted from Cordyceps militaris, which is a type of medicinal and nutritional food. Although Cor is considered to exert neuroprotective effects, its potential regulatory role in microglial function in AD, as well as the underlying specific mechanisms, remain poorly elucidated. Through transcriptomics combined with <em>in vitro</em> and<em> in vivo</em> experimental validations, we demonstrated that Cor suppresses the pro-inflammatory responses of MG while enhancing their phagocytic capacity for Aβ, with these dual effects mediated through distinct mechanisms. Specifically, on one hand, Cor can activate the STAT6 signaling pathway to upregulate the expression of phagocytosis-related factors, including Arg1, TGFβ, IL10, and CD206, thereby promoting the clearance of Aβ by MG. Notably, activation of the STAT6 signaling pathway does not affect the inflammatory response. On the other hand, Cor can inhibit caspase1 to reduce the levels of inflammation-related factors such as IL1β, TNFα, iNOS, and CD86, thereby alleviating neuroinflammation. Meanwhile, inhibition of caspase1 activity does not impact phagocytic function. This unique dual action of Cor, which simultaneously suppresses inflammation and promotes phagocytosis of MG, opens new avenues for prevention and therapeutic strategies against AD and provides solid experimental evidence and theoretical support.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2025.9250901.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147913962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of ovomucin on the physicochemical properties of <em>Lactobacillus plantarum</em> Lp90","authors":"Mei Wang, Hong He, Yu Wei, Xianhui Xiang, Jinqiu Wang, Shugang Li, Fang Geng","doi":"10.26599/fshw.2025.9250902","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250902","url":null,"abstract":"Egg white ovomucin (OVM) has properties highly similar to intestinal mucin and is considered to have a protective effect on probiotics, but this remains to be confirmed. This study evaluated the effect of OVM on the probiotic viability and functionality of <em>Lactobacillus plantarum </em>Lp90 and explored the mechanism by which OVM affects Lp90 using proteomics. The results indicated that OVM treatment showed no adverse effects on Lp90 viability, but concentration-dependently enhanced its self-aggregation ability, with the H-OVM group (10 mg/mL) reaching up to 39.6%. Quantitative proteomic analysis revealed that L-OVM (3 mg/mL) and H-OVM treatments resulted in the differential expression of only 79 (4.42%) and 173 (9.68%) proteins, respectively, indicating OVM exerted a weak overall effect on Lp90. Analysis of the differentially expressed proteins revealed that L-OVM enhanced the environmental adaptability of Lp90 by regulating cell wall synthesis and modification and promoting the expression of various nutrient transport systems. This study clarified that OVM is beneficial to the self-aggregation and environmental adaptability of Lp90, demonstrating that OVM has potential as a probiotic protectant.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveals the anticancer mechanism of luteoloside on colorectal cancer <em>in vivo and in vitro</em>","authors":"Jie Zhang, Xiuxiu Zhang, Wang-Wei Zhang, Qian Zhong, Kiran Thakur, Jian‐Guo Zhang, Guangsen Fan, Zhao‐Jun Wei","doi":"10.26599/fshw.2025.9250853","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250853","url":null,"abstract":"Colorectal cancer (CRC) is a malignant tumor characterized by high incidence and fatality rates, ranking as the second most common cancer globally. Honeysuckle, a traditional medicinal plant, contains a large amount of the compound called luteoloside (LUS), which exhibits notable biological functions, particularly in cancer prevention and treatment. This study aimed to elucidate the anticancer mechanisms targeted by LUS, focusing on the interaction between miRNA and mRNA within the context of CRC pathogenesis. We assessed the impact of LUS on CRC using both <em>in vivo</em> and <em>in vitro</em> methods. The potential mechanisms of LUS on CRC HCT116 cells were further explored using immunohistochemistry, immunofluorescence, miRNA-seq and mRNA-seq, western blot, and qRT-PCR. The integrated analysis identified a total of 4519 DEGs and 30 DEMs that exhibited negative regulatory interactions, suggesting that LUS triggered both apoptosis and autophagy via endoplasmic reticulum (ER) stress and DNA damage-related pathways. 87 DEGs and 29 DEMs corresponding to protein processing in endoplasmic reticulum (PPER) and 36 DEGs and 23 DEMs corresponding to p53 pathways were identified via miRNA-target gene network analysis. Hub miRNAs and genes, including Hsa-miR-152-3p, hsa-mir-663a-p3, hsa-mir-663b-p3, mmu-miR-6240_R-5_1ss1CA, CANX and PPM1D were identified as central regulators of anticancer potential of LUS. These findings suggest miRNA-mediated central gene regulatory networks can serve as predictive models for molecular mechanisms of CRC, potentially offering new understanding into the pathogenesis of CRC, indicating that LUS has broad market prospects in the development and application of functional food, medicine and other fields.&nbsp;","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite genetic characterization and bile acid metabolomics reveal deoxycholic acid protective effects on acute pancreatitis","authors":"L Wang, Xiaowu Dong, Qingtian Zhu, Zhi-Hao Wang, Ping Li, Ziyi Zhao, Weiwei Chen, Chenchen Yuan, Wei Li, Bo Tu, Weijuan Gong, Lei Zhang, Guotao Lu, Weixuan Yang","doi":"10.26599/fshw.2025.9250862","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250862","url":null,"abstract":"Abnormal bile acid metabolism is associated with various diseases, including acute pancreatitis (AP), although its beneficial effects have not been fully elucidated. This study aimed to investigate the relationship between deoxycholic acid (DCA) and AP.<strong> </strong>We designed a comprehensive analysis pipeline involving two-sample Mendelian randomization (MR) and targeted bile acid metabolomics based on a bi-center population to identify potential metabolic influencers of AP. These analyses were complemented by in vivo and in vitro experimental studies to assess the effects of metabolites, followed by integrated analysis and prediction of target molecules to explore the potential mechanisms underlying the protective effects of DCA.<strong> </strong>In MR studies, we identified a causal relationship between DCA and AP. A population-based targeted bile acid metabolomics showed that DCA levels were significantly reduced during the acute phase of AP compared with those in healthy controls and were associated with acute respiratory distress syndrome and infectious pancreatic necrosis. In the cholecystokinin-induced pancreatic acinar cell injury model and the caerulein-induced AP model, DCA administration alleviated pancreatic acinar cell injury, improved the pathological manifestations of pancreatic tissues, and reduced serum lipase and amylase levels. Integrated analysis of pancreatic transcriptomic data and DCA target proteins prediction by PharmMapper revealed significant alterations in the PPAR signaling pathway. Furthermore, Western blot and immunofluorescence assays confirmed that DCA likely mediates its protective effects through this mechanism.<strong> </strong>For the first time, we found that DCA is closely associated with the development of AP using a public database and a dual-center clinical cohort and that supplementation with DCA can improve acinar cell necrosis in AP, representing a promising novel strategy for the prevention and treatment of AP.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250862.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ketogenic diet disrupts BDH1-mediated ketone metabolism and causes myocardial dysfunction in mice","authors":"Yin Li, Bin Guo, Hui Shu, Hongyu Fu, Xiangpeng Liu, Jie Zhang, Yue Ma, Zhiwei Liu, Lei Fu, Yingying Dong, Xiaobo Sun, Bin Zhang, Zhihao Jia, Xiaosong Gu, Hong Sun, Feng Yu","doi":"10.26599/fshw.2025.9250852","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250852","url":null,"abstract":"Ketogenic diet (KD), recognized for its multiple metabolic benefits, such as enhanced glycemic homeostasis and weight loss, also imposes intricate influences on cardiac substrate utilization. While KD is effective in reducing blood glucose levels and improving insulin sensitivity, its long-term effects on myocardial ketone body (KB) metabolism remain insufficiently investigated, particularly in relation to cardiac remodeling. In the present study, we found that the KD-based nutritional intervention caused considerable cardiac hypertrophy, altered FAs and KBs metabolic pathways and impaired cardiac function, potentially through the repression of BDH1 protein, which is crucial for the utilization of β-hydroxybutyrate (β-OHB). Additionally, mice with cardiac muscle-specific <em>Bdh1</em> manipulation exhibited cardiac dysfunction after fasting, possibly due to histone methylation modifications. We established several diabetic models using streptozotocin (STZ), <em>db/db </em>transgenic mice, and a combination of STZ and a high-fat diet (HFD). We confirmed that these diabetic models exhibited significant alterations in cardiac ketone body metabolism and BDH1 expression. In summary, our study highlights that the downregulation of BDH1 induced by the ketogenic diet is associated with disrupted myocardial ketone utilization and may lead to myocardial structure and function impairment. Furthermore, the loss of BDH1 can also aggravate cardiac dysfunction during fasting by histone modifications.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250852.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}