Food Science and Human Wellness最新文献_第3页

Improved viability of trehalose on Lactobacillus plantarum embedded with whey protein concentrate/pullulan hydrogel in simulated gastrointestinal conditions and its application in juice 在模拟胃肠道条件下提高三卤糖对嵌入浓缩乳清蛋白/木聚糖的植物乳杆菌的活力及其在酸果汁中的应用

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250043

Xiujuan Wang , Haiyue Sun , Jian Wu , Yu Wang , Zhiyi Ai , Xinzhu Wang , Bo Nan , Yong Cao , Xia Li , Jingsheng Liu , Yuhua Wang

{"title":"Improved viability of trehalose on Lactobacillus plantarum embedded with whey protein concentrate/pullulan hydrogel in simulated gastrointestinal conditions and its application in juice","authors":"Xiujuan Wang , Haiyue Sun , Jian Wu , Yu Wang , Zhiyi Ai , Xinzhu Wang , Bo Nan , Yong Cao , Xia Li , Jingsheng Liu , Yuhua Wang","doi":"10.26599/FSHW.2023.9250043","DOIUrl":"10.26599/FSHW.2023.9250043","url":null,"abstract":"<div><div>Trehalose (TRE) was used to improve the gastrointestinal tolerance of <em>Lactobacillus plantarum</em> embedded with whey protein concentrate/pullulan (WPC/PUL) hydrogel and the embedded <em>L. plantarum</em> was applied to juice. The study indicated that 5 % TRE significantly increased the viable counts of <em>L. plantarum</em> embedded in WPC/PUL hydrogel from (8.83 ± 0.03) to (9.14 ± 0.04) (lg (CFU/g)) in simulated gastric juice (SGJ) and from (9.13 ± 0.04) to (9.38 ± 0.04) (lg (CFU/g)) in simulated intestinal juice, respectively. The addition of TRE improved the glass transition temperature of WPC/PUL hydrogel and decreased the hardness and its solubility in SGJ, which may be responsible for the improved protection of WPC/PUL hydrogels on <em>L. plantarum</em>. In addition, TRE increased the viable counts of <em>L. plantarum</em> in WPC/PUL probiotic microcapsule juice at low pH and high temperature during storage.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3614-3623"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polysaccharide-rich extract of Potentilla anserina ameliorates nonalcoholic fatty liver disease in free fatty acid-induced HepG2 cells and high-fat/sugar diet-fed mice 富含多糖的旱莲草提取物可改善游离脂肪酸诱导的 HepG2 细胞和高脂/糖饮食小鼠的非酒精性脂肪肝症状

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250020

Xiujun Lin , Yimei Zheng , Yingying Yan , Hongting Deng , Shunxin Wang , Yuanju He , Yuting Tian , Wenhui Zhang , Hui Teng

{"title":"Polysaccharide-rich extract of Potentilla anserina ameliorates nonalcoholic fatty liver disease in free fatty acid-induced HepG2 cells and high-fat/sugar diet-fed mice","authors":"Xiujun Lin , Yimei Zheng , Yingying Yan , Hongting Deng , Shunxin Wang , Yuanju He , Yuting Tian , Wenhui Zhang , Hui Teng","doi":"10.26599/FSHW.2023.9250020","DOIUrl":"10.26599/FSHW.2023.9250020","url":null,"abstract":"<div><div><em>Potentilla anserina</em> L. (PA) belongs to the Rosaceae family, is a common edible plant in the Qinghai-Tibet Plateau areas of China. This study elucidates the mechanism upon which crude polysaccharide of PA (PAP) on fat accumulation in HepG2 cells stimulated by oleic acid (OA) and high fat high sugar induced mice. The result revealed that PAP inhibited lipid accumulation in obese mice and ameliorated the degree of damage in OA-induced HepG2 cells. Specifically, compared to the control group, the TG and TC levels were decreased in cells and mice serum, the aspartate transaminase and alamine aminotransferase contents were declined in liver of obese mice by PAP treatment. The expressions of adipogenic genes of <em>SREBP-1c</em>, <em>C/EBPα</em>, <em>PPARγ</em>, and <em>FAS</em> were inhibited after PAP treatment. Moreover, PAP increased the mRNA levels of <em>CPT-1</em> and <em>PPARα</em>, which were involved in fatty acid oxidation. The present results indicated the PAP could alleviate the damage of liver associated with obesity and PAP treatment might provide a dietary therapeutic option for the treatment of hyperlipidemia.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3351-3360"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140467677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative mechanism of chicken wooden breast myofibrillar protein 鸡木胸肌纤维蛋白的氧化机制

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250026

Ke Wang , Yimin Zhang , Jingxin Sun

{"title":"Oxidative mechanism of chicken wooden breast myofibrillar protein","authors":"Ke Wang , Yimin Zhang , Jingxin Sun","doi":"10.26599/FSHW.2023.9250026","DOIUrl":"10.26599/FSHW.2023.9250026","url":null,"abstract":"<div><div>To explore the oxidation mechanism of wooden breast myofibrillar protein (WBMP), oxidative breast MP (OBMP) was obtained from different doses (3, 10, and 20 mmol/L) of H<sub>2</sub>O<sub>2</sub> oxidized normal breast MP (NBMP). The results showed that the Zeta-potential, particle size, solubility, sulfhydryl, and carbonyl contents of OBMP-3 (3 mmol/L, low-dose free radicals) and WBMP were similar. Fluorescence spectrum analysis showed that the oxidation of low-dose free radicals led to a significant increase in the surface hydrophobicity (from 214.03 ± 10.03 to 393.50 ± 10.33) and tryptophan fluorescence intensity (from 185.71 to 568.32). In addition, the <em>α</em>-helix content of WBMP decreased significantly from (37.46 ± 1.15)% (NBMP) to (34.70 ± 2.04)%, while <em>β</em>-sheet and random coil contents increased significantly (<em>P</em> < 0.05) from (14.37 ± 0.69)% and (22.24 ± 0.78)% (NBMP) to (17.70 ± 0.87)% and (25.20 ± 1.47)% (WBMP). In summary, low-dose free radical oxidation attacks protein groups, inducing secondary and tertiary structural changes, leading to the formation of WBMP. This work will provide a theoretical basis at the molecular level for exploring the mechanism of functional degradation of WBMP.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3415-3423"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adulteration detection of plant protein beverages by UPLC-MS/MS based on signature peptide of allergen 基于过敏原特征肽的 UPLC-MS/MS 检测植物蛋白饮料的掺假情况

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250022

Yawei Ning , Zhuo Liu , Zheng Yang , Junmei Ma , Yan Zhang , Qiang Li

{"title":"Adulteration detection of plant protein beverages by UPLC-MS/MS based on signature peptide of allergen","authors":"Yawei Ning , Zhuo Liu , Zheng Yang , Junmei Ma , Yan Zhang , Qiang Li","doi":"10.26599/FSHW.2023.9250022","DOIUrl":"10.26599/FSHW.2023.9250022","url":null,"abstract":"<div><div>Plant protein beverage adulteration occurs frequently, which may cause health problems for consumers due to the hidden allergens. Hence, a novel method was developed for authentication by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Almond, peanut, walnut and soybean were hydrolyzed, followed by separation by NanoLC-Triple TOF MS. The obtained fingerprints were identified by ProteinPilot<sup>TM</sup> combined with Uniprot, and 16 signature peptides were selected. Afterwards, plant protein beverages treated by trypsin hydrolysis were analyzed with UPLC-MS/MS. This method showed a good linear relationship with <em>R</em><sup>2</sup> > 0.994 03. The limit of quantification were 0.015, 0.01, 0.5 and 0.05 g/L for almond, peanut, walnut and soybean, respectively. Mean recoveries ranged from 84.77 % to 110.44 % with RSDs < 15 %. The developed method was successfully applied to the adulteration detection of 31 plant protein beverages to reveal adulteration and false labeling. Conclusively, this method could provide technical support for authentication of plant protein beverages to protect the rights and health of consumers.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3371-3380"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diet with high content of advanced glycation end products induces oxidative stress damage and systemic inflammation in experimental mice: protective effect of peanut skin procyanidins 高级糖化终产物含量高的饮食会诱发实验小鼠的氧化应激损伤和全身炎症：花生皮原花青素的保护作用

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250039

Li Zhao , Xingxing Zhang , Langzhi He , Yubing Li , Yue Yu , Qun Lu , Rui Liu

{"title":"Diet with high content of advanced glycation end products induces oxidative stress damage and systemic inflammation in experimental mice: protective effect of peanut skin procyanidins","authors":"Li Zhao , Xingxing Zhang , Langzhi He , Yubing Li , Yue Yu , Qun Lu , Rui Liu","doi":"10.26599/FSHW.2023.9250039","DOIUrl":"10.26599/FSHW.2023.9250039","url":null,"abstract":"<div><div>Non-enzymatic glycation reaction in food can produce diet-derived advanced glycation end products (dAGEs), which have potential health risks. Thus, it is of great significance to find efficient substances to improve the negative effects induced by dAGEs on human health. This study investigated the intervening effects of peanut skin procyanidins (PSP) on the dAGEs-induced oxidative stress and systemic inflammation in experimental mice model. Results showed that the accumulation of AGEs in serum, liver, and kidney was significantly increased after mice were fed dAGEs (<em>P</em> < 0.05). The expression of advanced glycation product receptor (RAGE) was also significantly increased in liver and kidney (<em>P</em> < 0.05). PSP could not only effectively reduce the accumulation of AGEs in serum, liver and kidney of mice, but also reduce the expression of RAGE in liver and kidney of mice. And the levels of pro-inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and IL-1β in serum of mice were significantly decreased (<em>P</em> < 0.05), while the levels of anti-inflammatory factor IL-10 were increased, and the inflammatory injury in mice was improved. In addition, the levels of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) in liver and kidney of mice were increased (<em>P</em> < 0.05), and the level of malondialdehyde (MDA) was decreased (<em>P</em> < 0.05), which enhanced the antioxidant capacity of mice <em>in vivo</em>, and improved the oxidative damage of liver and kidney. Molecular docking technique was used to confirm that the parent compound of procyanidins and its main metabolites, such as 3-hydroxyphenylacetic acid, could interact with RAGE, which might inhibit the activation of nuclear transcription factor (NF-κB), and ultimately reduce oxidative stress and inflammation in mice.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3570-3581"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140467433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of feed additive ethoxyquin on tilapia growth performance and potential secondary hazard to consumers 饲料添加剂乙氧基喹对罗非鱼生长性能的影响及对消费者的潜在次生危害

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250010

Shanjun Chen , Chong Wang , Yanan Liu , Zhigang Zhou , Linglin Fu , Yanbo Wang

{"title":"The effect of feed additive ethoxyquin on tilapia growth performance and potential secondary hazard to consumers","authors":"Shanjun Chen , Chong Wang , Yanan Liu , Zhigang Zhou , Linglin Fu , Yanbo Wang","doi":"10.26599/FSHW.2023.9250010","DOIUrl":"10.26599/FSHW.2023.9250010","url":null,"abstract":"<div><div>Ethoxyquin (EQ) is a widely used feed additives for aquaculture, but the potential hazard to fish and consumers are not fully understood. In this work, EQ was applied to tilapia fed with either high-fat or low-fat feed, and the changes in growth performance and intestinal barrier integrality of genetically improved farmed tilapia was observed. Additionally, the microbiota in the intestinal content and flesh was analyzed by 16S rDNA sequencing, and the flesh metabolites was measured by LC-MS/MS untargeted metabolomics. In the low-fat group, EQ increased weight gain and feed conversion rate, but reduced survival rate. In the high-fat group, only weight gain decreased, and there was no significant effect on feed conversion rate and survival rate. Additionally, the intestinal villi and microbiota diversity was impaired in both feeding conditions. Microbiota analysis revealed that EQ resulted in different composition and lower diversity of the flesh microbiota and upregulated the proportion of potential pathogens. EQ also changed the flesh metabolome, decreasing beneficial metabolites and increasing potential harmful components. This work elucidated the effect of EQ on growth performance of tilapia and implied that it has adverse effects on tilapia and may be passed on to consumers.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3236-3244"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the quality deterioration induced by microorganisms in Litopenaeus vannamei during superchilling storage 万年青在超冷贮藏期间由微生物引起的质量下降的启示

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250030

Chuang Pan , Kaiying Yang , Xianqing Yang , Haiyun Wu , Chunsheng Li , Shuxian Hao , Yongqiang Zhao , Shengjun Chen , Xiaoshan Long

{"title":"Insight into the quality deterioration induced by microorganisms in Litopenaeus vannamei during superchilling storage","authors":"Chuang Pan , Kaiying Yang , Xianqing Yang , Haiyun Wu , Chunsheng Li , Shuxian Hao , Yongqiang Zhao , Shengjun Chen , Xiaoshan Long","doi":"10.26599/FSHW.2023.9250030","DOIUrl":"10.26599/FSHW.2023.9250030","url":null,"abstract":"<div><div>Quality deterioration induced by microbial community in <em>Litopenaeus vannamei</em> during superchilling storage were demonstrated in this study. In this study, 6 microorganisms were identified as the biomarkers by 16S rDNA sequencing and 71 differential metabolites were identified by untargeted metabolomics. The correlation analysis between microorganisms and differential metabolites revealed that <em>Oceanicaulis</em>, <em>Aliihoeflea</em>, <em>Prauserella</em>, <em>Chelativorans</em> and <em>Pseudoalteromonas</em> had the potential to be the important microorganisms of deterioration of superchilled shrimp. Moreover, the spearman correlation analysis revealed that lightness value had highly significant correlation with <em>Shewanella</em> (<em>P</em> < 0.01), and thiobarbituric acid content, total viable count value, total sulfhydryl content, trichloroacetic acid-soluble peptide and Ca<sup>2+</sup>-ATPase activity showed significantly correlation with <em>Oceanicaulis</em>, <em>Prauserella</em>, <em>Chelativorans</em>, <em>Aliihoeflea</em> and <em>Pseudoalteromonas</em> (<em>P</em> < 0.05). This study offers a better understanding for mechanisms behind quality degradation induced by microorganisms, which may help the quality maintaining of Pacific white shrimp during superchilling storage.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3461-3471"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of short-term changes in sleeping and eating patterns on glucometabolic health and gut microbiota in healthy young adults: a proof-of-concept controlled feeding study 短期改变睡眠和饮食模式对健康年轻人的糖代谢健康和肠道微生物群的影响：概念验证对照喂养研究

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250038

Jiehua Chen , Ruijie Zhang , Chao Zhou , Louise Weiwei Lu , Dana Feng , Haiqiao Zou , Ran Gao , Xinying Zhang , Peiyi Chen , Jiayue Zhu , Haoxie Xu , Nina Zeng , Cijuan Zhang , Bin Liu , Mingfu Wang , Qian Ge , Caiqun Ouyang , Feng Chen

{"title":"The impact of short-term changes in sleeping and eating patterns on glucometabolic health and gut microbiota in healthy young adults: a proof-of-concept controlled feeding study","authors":"Jiehua Chen , Ruijie Zhang , Chao Zhou , Louise Weiwei Lu , Dana Feng , Haiqiao Zou , Ran Gao , Xinying Zhang , Peiyi Chen , Jiayue Zhu , Haoxie Xu , Nina Zeng , Cijuan Zhang , Bin Liu , Mingfu Wang , Qian Ge , Caiqun Ouyang , Feng Chen","doi":"10.26599/FSHW.2023.9250038","DOIUrl":"10.26599/FSHW.2023.9250038","url":null,"abstract":"<div><div>Epidemiological studies showed that night workers are at higher risk of developing chronic metabolic diseases. However, no study has investigated the changes in circadian rhythms caused by a combined effect of sleep and diet in a real-life setting on cardiometabolic health, gut microbiota, and psychological status in healthy people. A 4-week step-wise misaligned-realigned controlled-feeding trial with a 2 × 2 factorial design (sleep and diet) was conducted on healthy young adults. At first, subjects experienced a one-week circadian rhythm misalignment with a high-fat fast-food diet, extended eating window, and delayed sleep schedules, then gradually transited to a complete circadian rhythm realignment with a high-fiber balanced diet, 8-h time-restricted eating, and normal sleep schedules. Circadian rhythm misalignment led to significantly higher levels of fasting glucose and homeostatic model assessment for insulin resistance (HOMA-IR) of subjects compared to baseline and failed to recover to the baseline level in circadian rhythm realignments. Notably, the incremental area under the curve (iAUC) of postprandial glucose decreased with circadian rhythm adjustments as compared to that in circadian rhythm misalignment, suggesting circadian rhythm realignment by sleep or/and diet could partly restore glucose metabolism impaired by a short-term circadian rhythm misalignment. However, circadian rhythm changes did not result in overall perturbations of gut microbiota diversities.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3553-3569"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of Lactobacillus fermentum isolated from individuals following vegan, omnivorous and high-meat diets on ulcerative colitis in mice 从素食、杂食和高肉饮食者体内分离的发酵乳杆菌对小鼠溃疡性结肠炎的改善作用

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250005

Qingsong Zhang , Wei Xiao , Leilei Yu , Fengwei Tian , Jianxin Zhao , Hao Zhang , Wei Chen , Qixiao Zhai

{"title":"Ameliorative effects of Lactobacillus fermentum isolated from individuals following vegan, omnivorous and high-meat diets on ulcerative colitis in mice","authors":"Qingsong Zhang , Wei Xiao , Leilei Yu , Fengwei Tian , Jianxin Zhao , Hao Zhang , Wei Chen , Qixiao Zhai","doi":"10.26599/FSHW.2023.9250005","DOIUrl":"10.26599/FSHW.2023.9250005","url":null,"abstract":"<div><div><em>Lactobacillus</em> spp. can be beneficial for the prevention or treatment of ulcerative colitis (UC). In this study, 153 participants who followed vegan, omnivorous, or high-meat diet were recruited. Compositional analysis of the <em>Lactobacillus</em> community in feces revealed that <em>Lactobacillus fermentum</em> strains were significantly affected by diet. Administration of mixed <em>L. fermentum</em> strains from vegans significantly improved inflammation compared to that from omnivores and high-meat consumers, as evidenced by a significant reduction in colonic tissue damage, improvement in inflammatory cytokines, enhanced expression of ZO-1, occludin, and claudin-3, and a significant increase in short chain fatty acids concentration. The effect of a single strain of <em>L. fermentum</em> was similar to that of a mixed strains of <em>L. fermentum</em> group. Genomic analysis suggested that <em>L. fermentum</em> strains from the guts of vegans possessed a higher prevalence of genes involved in carbohydrate catabolism than those from the guts of omnivores and high-meat eaters. In particular, the <em>ME2</em> gene is involved in the biosynthesis of acetate, a compound considered to possess anti-inflammatory properties. In conclusion, this study indicates strain-speciﬁc differences in the ability of <em>L. fermentum</em> strains to alleviate UC in mice, influenced by habitual diets.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3181-3192"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140464198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel fluorescence sensor for milk clotting enzyme chymosin using peptide as substrate and covalent organic framework nanosheet as fluorescence quencher 以肽为底物、共价有机框架纳米片为荧光淬灭剂的新型牛奶凝血酶糜蛋白酶荧光传感器

IF 5.6 1区农林科学

Food Science and Human Wellness Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250042

Xiaokang Lu , Tianle Qi , Linjiang Guo , Lin Xiao , Hanbin Xu , Guobao Ning , Hui Zhao , Canpeng Li

{"title":"A novel fluorescence sensor for milk clotting enzyme chymosin using peptide as substrate and covalent organic framework nanosheet as fluorescence quencher","authors":"Xiaokang Lu , Tianle Qi , Linjiang Guo , Lin Xiao , Hanbin Xu , Guobao Ning , Hui Zhao , Canpeng Li","doi":"10.26599/FSHW.2023.9250042","DOIUrl":"10.26599/FSHW.2023.9250042","url":null,"abstract":"<div><div>Chymosin is one of the critical enzymes in cheese making. Herein, we proposed a novel fluorometric assay for chymosin determination. Firstly, covalent organic frameworks (COF) were synthesized and exfoliated to 2-dimensional COF nanosheets (COF NS) by ultrasound treatment. Gold nanoparticles (Au NPs) were loaded with COF NS to prepare AuNPs/COF NS (Au@COF NS). Secondly, rhodamine B (RhB) modified substrate peptide (Pep) for chymosin was linked with Au@COF NS to construct a Pep-Au@COF NS nanocomposite. For the sensing principle, fluorescence of RhB was quenched by Au@COF NS and the fluorescence intensity was weak due to the fluorescence resonance energy transfer between COF NS and RhB of Pep. However, in the presence of chymosin, the RhB was released by specific cleavage of the substrate peptide by chymosin and resulted in the recovery of fluorescence. The increased fluorescence intensity was proportional to the increase of chymosin concentration and thus a “turn on” fluorescent sensor for chymosin was constructed. The sensor showed a linear range in the concentration of 0.05<img>60.00 μg/mL for the detection of chymosin with a detection limit of 20 ng/mL. The sensor was used to quantify chymosin in rennet product with good selectivity, which has the potential applications in cheese manufacturing.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3606-3613"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140464922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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