Food Science and Human Wellness最新文献

{"title":"Naringenin Alleviates Acute Ethanol Exposure-induced Nerve Injury Through Regulating Neuroinflammation via PI3K/AKT Pathway-Mediated Modulation of Microglial M1/M2 Polarization","authors":"Changming Yang, Hongxi Zheng, Han Wang, Yinjia He, Xiaodong Liu, Linqing Miao","doi":"10.26599/fshw.2026.9251116","DOIUrl":"https://doi.org/10.26599/fshw.2026.9251116","url":null,"abstract":"","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148305719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remodeling of macrophage lipid metabolism by phytosterol suppresses inflammation: Insights from targeted lipidomics","authors":"X. Wang, Lili Xu, Yanhong Zhang, Aizhen Zong, Fangling Du, Hao Yue, Tongcheng Xu","doi":"10.26599/fshw.2026.9251026","DOIUrl":"https://doi.org/10.26599/fshw.2026.9251026","url":null,"abstract":"Phytosterols are widely recognized bioactive compounds exhibiting anti-inflammatory effects. Macrophages adopt a distinct lipid metabolic state during inflammatory responses. However, the specific mechanisms by which phytosterols influence macrophage lipid metabolism and subsequently regulate inflammation through metabolic remodeling remain unclear. In this study, we elucidate how phytosterols reprogram macrophage lipid metabolism to attenuate inflammatory responses. Quantitative lipidomic analysis revealed that phytosterol-induced lipid remodeling predominantly affects long-chain triglycerides. Further mechanistic studies demonstrated that phytosterols increase lipid droplet accumulation and regulate their metabolism by suppressing adipose triglyceride lipase (ATGL), upregulating hypoxia inducible lipid droplet associated proteins (HILPDA), and reducing the production of pro-inflammatory intermediates, including arachidonic acid. Consequently, phytosterol treatment decreased prostaglandin E₂ (PGE₂) levels and reduced the secretion of pro-inflammatory cytokines such as IL-1β. Collectively, these findings provide novel mechanistic insights into how phytosterols reduce macrophage-driven inflammation through lipid metabolic reprogramming.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2026.9251026.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chicory Sesquiterpene Lactones Trained Faeces-derived Extracellular Vesicles Alleviated Ulcerative Colitis by Modulating Gut Microbiota and Bile Acids Metabolism","authors":"Tunyu Jian, Zhen Tang, Yuwen Tian, Weichen Zhang, Xiaoqin Ding, Xiuhua Meng, Han Lv, Bei Tong, Yanan Gai, Weilin Li, Lan Wu, Guanting Niu, Jian Chen","doi":"10.26599/fshw.2026.9251045","DOIUrl":"https://doi.org/10.26599/fshw.2026.9251045","url":null,"abstract":"<p class=\"0000\" style=\"margin-top: 6.0pt;\"><span lang=\"EN-US\">The gut microbiota communicates with the host via extracellular vesicles (EVs), offering a promising therapeutic target for ulcerative colitis (UC). This study investigates the therapeutic potential of sesquiterpene lactones (SL) from chicory and the EVs derived from the faeces of SL-treated mice (SL-trained FEVs) in a dextran sulfate sodium (DSS)-induced UC model. Oral administration of SL significantly ameliorated colitis symptoms, reduced intestinal inflammation, preserved intestinal barrier integrity by maintaining tight junction proteins, and attenuated colonic tissue damage. These protective effects were mechanistically linked to the attenuation of gut microbiota dysbiosis and the restoration of bile acid metabolism homeostasis. Notably, gavage of SL-trained FEVs recapitulated the therapeutic benefits of direct SL treatment, demonstrating the critical role of microbiota-derived EVs in mediating SL's effects. Profiling revealed that SL treatment enriched specific microRNAs (e.g., miR-26a-5p, miR-200b-3p, and miR-194-5p) within these FEVs. Both SL and SL-trained FEVs induced comparable and significant shifts in the gut microbiota composition, notably modulating the relative abundance of genera including <em>Odoribacter</em>, <em>Oscillibacter</em>, <em>Escherichia-Shigella</em>, <em>g-Clostridia_UCG-014_unclassified</em>, and phyla such as p-Actinobacteriota and p-Patescibacteria. Concomitantly, both interventions significantly altered key bile acid profiles, affecting levels of taurocholic acid (TCA), lithocholic acid (LCA), nor-deoxycholic acid (NorDCA), and taurochenodeoxycholic acid (TCDCA). In conclusion, our findings highlight SL and SL-trained FEVs as promising agents for UC treatment, acting through the coordinated modulation of the gut microbiota-bile acid axis, and propose FEVs as a novel, targeted delivery system for microbiota-based therapeutics.</span>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2026.9251045.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive identification and semi-quantitative profiling of 3,5,6,7,8,3&amp;prime;,4&amp;prime;-heptamethoxyflavone metabolites in rats by UHPLC-LTQ-Orbitrap mass spectrometry","authors":"Konglong Feng, Yilu Chen, Mingyue Song, Yu Song, Guo Liu, Meng Kang, Chi-Tang Ho, Hang Xiao, Yong Cao","doi":"10.26599/fshw.2026.9250992","DOIUrl":"https://doi.org/10.26599/fshw.2026.9250992","url":null,"abstract":"3,5,6,7,8,3′,4′-Heptamethoxyflavone (HMF), one of the highest <em>O</em>-methyl numbers in the polymethoxyflavones, possesses various significant health benefits. However, the <em>in vivo</em> metabolic profile of HMF remains largely unexplored. In this study, a systematic identification and relative quantitation of HMF and its metabolites in rats were performed using UHPLC-LTQ-Orbitrap mass spectrometry combined with enzymatic hydrolysis. A total of 56 metabolites were tentatively identified, including 37 demethylated metabolites and 19 glucuronide conjugates of these demethylated products. Notably, all seven mono-demethylated metabolites of HMF were simultaneously detected and structurally discriminated for the first time in rats, along with other di-, tri-, tetra-, and penta-demethylated metabolites. Semi-quantitative analysis revealed that HMF underwent predominant biotransformation into mono-demethylated metabolites in rats, followed by progressive sequential demethylation of these metabolites to generate more di-, tri-, tetra-, and penta-demethylated metabolites. Demethylation and glucuronidation are the primary metabolic pathways of HMF <em>in vivo</em>. This study presents the first comprehensive elucidation of the <em>in vivo</em> metabolic profile of HMF, which is helpful for further understanding of its <em>in vivo</em> potential effective components and pharmacological mechanism.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2026.9250992.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procyanidin B2 improves insulin resistance by regulating the IRS1/PI3K/AKT-Nrf2-GSK3&amp;beta; based on computational biology, molecular evidence and direct binding proof","authors":"Wenli Yang, Shiyun Chen, Rong Tong, Yuhang Yi, Dongsheng Wang, Fangfang Yan, Tao Li, Chenghao Lv, Si Qin","doi":"10.26599/fshw.2026.9250965","DOIUrl":"https://doi.org/10.26599/fshw.2026.9250965","url":null,"abstract":"Procyanidin B2 (PB2), a dimer of oligomeric procyanidins, has been reported to ameliorate abnormal glucose metabolism; however, its efficacy in regulating redox-related insulin resistance (IR) and underlying mechanism has not yet been studied. Currently, antioxidant activity and digestive enzyme inhibitory tests were applied to screen the preliminary chemopreventive effect of PB2 on glucose metabolism dysfunction, further computational biology and molecular verification to elucidate its mechanism on improving IR. The results indicated that PB2 had limited antioxidant property against ROS and inhibitory property on digestive enzyme. Network pharmacology analysis implied that PI3K/AKT and FoxO pathways were the main targeted by PB2 to combating IR. Further molecular data demonstrated that PB2 promoted glycogen synthesis by activation of PI3K/AKT-Nrf2 signaling pathway via promoting posttranscriptional protein stability, nuclear translocation of Nrf2 and ARE binding ability, and inhibited gluconeogenesis by stimulating FoxO1 phosphorylation to reduce the expression of downstream G6Pase. Innovatively, molecular docking coupled with different Pull-Down assays revealed that PB2 could directly bind with AKT, Nrf2 and GSK3β, suggesting PB2 may improve IR via regulation of PI3K/AKT-Nrf2-GSK3β signaling, more than FoxO1 signaling pathway; subsequently, Nrf2 could directly bind with GSK3β, reinforcing evidence for PB2-Nrf2-GSK3β complex formation. Besides, co-treatment with PI3K/AKT and Nrf2 inhibitors or siRNAs highlighted the essential role of Nrf2 activation in PB2-contributed IR improvement. Moreover, PB2 has also been validated to exert its effect via regulation of PI3K/AKT-Nrf2-GSK3β signaling pathway <em>in vivo</em>. Therefore, these findings suggest that PB2 is a potent and promising compound to ameliorate glucose metabolism by targeting IR.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honey as a natural absorption enhancer: Mechanisms underlying the improved oral bioavailability and bioactivity of\u0000 <em>Astragalus</em>\u0000 flavonoids","authors":"Weiye Zhang, Xiao Su, Guangmin Liu, Yanjun Yang, Zhubin Zhang, Xiaobin Jia, Liang Feng, Bing Yang","doi":"10.26599/fshw.2026.9250943","DOIUrl":"https://doi.org/10.26599/fshw.2026.9250943","url":null,"abstract":"","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Emerging Strategy for Grading Chun Mee Green Tea by Combination of Metabolites, Sensory Properties, and Bioactivities","authors":"Libin Chen, Jia‐Ping Ke, Guoping Lai, Wen, Piaopiao Long, Liang Zhang, Zisheng Han","doi":"10.26599/fshw.2026.9251014","DOIUrl":"https://doi.org/10.26599/fshw.2026.9251014","url":null,"abstract":"","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tea-derived exosome-like nanoparticles ameliorate atopic dermatitis by restoring gut microbiome homeostasis via enrichment of Parabacteroides goldsteinii","authors":"Chih‐Jung Chang, Ching-ping Su, Jian Zhou, Qi-Wen Ma, Wen-Hung Chung, Chun-Bing Chen, Chuang-Wei Wang, Maoli Chen, Liang Chen, Yunzhi Gao, Chien-Chun Liu","doi":"10.26599/fshw.2026.9250946","DOIUrl":"https://doi.org/10.26599/fshw.2026.9250946","url":null,"abstract":"Atopic dermatitis (AD), a chronic inflammatory skin disorder often associated with gut microbiota dysbiosis, remains without safe and effective therapies. Tea-derived exosome-like nanoparticles (TELNs) possess anti-inflammatory and intestinal barrier–protective properties, yet their potential in AD has not been explored. In this study, TELNs were successfully isolated from tea leaves, with an average diameter of 77.1 nm and enriched in diverse functional proteins and metabolites. TELNs remained stable within the gastrointestinal tract and demonstrated therapeutic efficacy against AD. <em>In vitro</em>, TELNs were biocompatible and markedly suppressed inflammatory cytokines (TNF-α, IL-6) and chemokines (TARC, RNATES), while inhibiting NF-κB and JAK/STAT signaling pathways. In an AD mouse model, oral administration of TELNs alleviated clinical symptoms, improved skin barrier function, and reduced inflammation through these pathways. Mechanistically, TELNs restored gut microbiota imbalance induced by AD, notably enriching<em> Parabacteroides goldsteinii </em>(P<em>. goldsteinii</em>). Further experiments revealed that TELNs significantly promoted the growth of <em>P. goldsteinii</em> <em>in vitro</em>. Oral supplementation with <em>P. goldsteinii</em> effectively mitigated clinical symptoms and barrier dysfunction in AD mice, accompanied by reduced inflammatory cytokine production and suppression of NF-κB and JAK/STAT activation. Collectively, these findings demonstrate that TELNs ameliorate AD by modulating immune responses and reshaping gut microbiota, particularly through the enrichment of <em>P. goldsteinii</em>. This study highlights TELNs as promising, naturally derived nanotherapeutics for AD.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Prebiotic Activity and Oxidative Damage Protection for Exopolysaccharides Derived from Liquid Fermented <em>Poria cocos</em>","authors":"Lei Zhang, Yongqi Liu, Quan Gan, Wei Zhou, Xiaoting Wu, Jiamiao Liu, Guangyue Shen, Peipei Han, Zhenxing Liu, Shiru Jia, Limin Hao","doi":"10.26599/fshw.2025.9250903","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250903","url":null,"abstract":"<em>Poria cocos</em> is a traditional Chinese medicinal and edible fungus. <em>Poria cocos</em> polysaccharides possess biological activities such as antioxidant and anti-inflammatory effects, making them a novel resource for functional foods. To promote the industrial application of <em>Poria cocos</em> polysaccharides in functional foods, this study utilized liquid fermentation technology to prepare <em>Poria cocos</em> exopolysaccharides (PCE) and compared them with <em>Poria cocos</em> fruiting body polysaccharides (PCP) in terms of intestinal microbiota regulation and <em>in vivo</em> oxidative damage protection. The results showed that PCE contained (53.98 ± 0.19)% of total sugars as well as (6.64 ± 0.51)% of proteins. Two fractions (PCE-0 and PCE-0.2) were obtained by purification of PCE, which had smooth clustered and rough flaky microscopic morphology, respectively. Both of them were composed of glucose, mannose, galactose and other monosaccharides, with β-glycosidic bonds and higher branched structures. PCE-0 contained a triple helical structure while PCE-0.2 did not. <em>In vivo</em> results showed that PCE increased the abundance of probiotics such as <em>Bacteroides</em>, <em>Oscillospira</em>, and <em>Akkermansia</em> in the mouse intestine, exerting a positive influence on maintaining intestinal homeostasis. Additionally, PCE effectively enhanced the activity of superoxide dismutase and glutathione peroxidase in the serum, liver and jejunum of oxidative-damaged mice. It also reduced malondialdehyde content, improved total antioxidant capacity, and regulated the levels of IL-1β, IL-6, IL-10, TNF-α. These effects protected the mice against oxidative damage and inflammation caused by alcohol-induced liver injury. Further molecular mechanism studies suggest that PCE alleviates alcohol-induced liver injury through activation of the Keap1/Nrf2 signaling pathway. This study aimed to explore the potential application of liquid fermented <em>Poria cocos</em> polysaccharides in functional foods, providing a reference for the development and utilization of liquid fermentation products from edible fungi.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"W/O Emulsion Encapsulated Oligopeptide (KRQKYD) for Ameliorating Alcohol-induced Liver Damage by Regulating the Intestinal Homeostasis","authors":"Wen Nie, Yeye Du, Yu-fang Luo, Yue Wang, Su-ting Gao, Baocai Xu","doi":"10.26599/fshw.2025.9250883","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250883","url":null,"abstract":"Alcohol-induced liver damage (ALD) remains a global health burden with limited therapeutic options. To address this challenge, this study developed a water-in-oil (W/O) emulsion encapsulating the oligopeptide KRQKYD, using Jinhua ham fat (JHF) as the oil phase, aiming to enhance the oligopeptide’s digestive stability and ameliorate ALD by regulating intestinal homeostasis. The results confirmed that the W/O emulsion (containing 60% oligopeptide solution) had high encapsulation efficiency (96.37% ± 9.43%), which significantly enhanced the gastrointestinal digestion stability of KRQKYD—with 86.48% ± 2.20% of KRQKYD retained after 120 minutes of simulated intestinal digestion, a rate markedly higher than the 60.84% ± 1.84% retention of free KRQKYD. Moreover, in C57BL/6 mouse models of ALD, oral administration of the emulsion (400 mg/kg B.W., containing 60% oligopeptide solution at 55.6% w/w) significantly reduced serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and malondialdehyde (MDA) compared to ethanol-treated mice (<em>P</em> &lt; 0.05), with efficacy superior to that of KRQKYD or JHF alone. Mechanistically, the emulsion exerted multifaceted protective effects through synergistic actions of its components: KRQKYD promoted the proliferation of <em>Akkermansia muciniphila</em> by alleviating intestinal oxidative stress, while JHF enriched <em>Lactobacillus</em> by replenishing long-chain fatty acids, collectively restoring microbial balance (e.g., reducing <em>Proteobacteria</em> and increasing <em>Verrucomicrobia</em>). Concurrently, KRQKYD upregulated the <em>NRF2/HO-1</em> pathway, and JHF activated the <em>AhR/NQO1</em> pathway, which together reduced hepatic ROS accumulation and enhanced the activities of SOD and GSH-Px. Additionally, the emulsion strengthened intestinal barrier function by increasing Occludin and Claudin-1 expression, thereby reducing LPS leakage; this, in turn, inhibited the <em>LPS-TLR4-NF-κB</em> cascade and lowered hepatic levels of pro-inflammatory cytokines (TNF-<em>α</em>, IL-1<em>β</em>, IL-6). Furthermore, in terms of lipid metabolism, the emulsion downregulated SREBP1c-mediated lipid synthesis and activated AMPK-dependent lipid oxidation, ultimately decreasing hepatic triglyceride accumulation. This study demonstrates that the W/O emulsion enhances KRQKYD stability and exerts synergistic effects with JHF, providing a novel strategy for the oral delivery of bioactive oligopeptides to ameliorate ALD by targeting gut-liver crosstalk.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}