Food Science and Human Wellness最新文献

{"title":"<em>In vitro</em> fermentation characteristics of guar gum: Particularly enriching <em>Bifidobacterium pseudolongum</em> and <em>Parabacteroides distasonis</em>","authors":"Shanshan Zhang, Yonggan Sun, Yanli Zhang, Shaoping Nie","doi":"10.26599/fshw.2025.9250896","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250896","url":null,"abstract":"Guar gum, a widely used food hydrocolloid, exhibits extensive biological activities and its regulatory effects on the gut microbiota are closely linked to health outcomes. However, its specific enrichment effects on gut microbiota and the interaction modes with the enriched bacteria remain unclear. This study investigated the regulatory effects using an <em>in vitro</em> fermentation model and both single-step and stepwise cultivation methods. Results showed that the gut microbiota degraded guar gum, yielding abundant acetate and propionate. Guar gum suppressed the growth of the potential pathogenic bacteria (Enterobacteriaceae) while enriching the beneficial bacteria (<em>Bifidobacterium </em>and <em>Parabacteroides</em>). Notably, <em>B. pseudolongum </em>and <em>P. distasonis</em> were specifically enriched, although neither could directly utilize guar gum. The primary degrader, such as <em>Bacteroides ovatus</em>, facilitated guar gum breakdown and stimulated the proliferation of <em>B. pseudolongum </em>and <em>P. distasonis</em>. Further analysis revealed that the proliferation of these bacteria may be related to their preferences for utilizing mannose and galactose. Taken together, these findings enhance our understanding of the interactions between guar gum and the microbiota, and contribute to the scientific basis for developing guar gum-based functional foods.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250896.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147912777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compartmentalized multi-enzyme immobilization in hierarchical shell-shell structure for glucose detection","authors":"Lixue Zhao, Minsong Zhang, Shao Yang, Tongyue Zhu, Zichen Wang, Y.J Bi, Hongwu Wang, Jiandong Cui","doi":"10.26599/fshw.2025.9250839","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250839","url":null,"abstract":"Recent years, incorporation of multiple enzymes into matrix is considered as promising candidates for constructing highly sensitive glucose-responsive systems. However, the stochastic nature of traditional multi-enzyme immobilization techniques makes it challenging to precisely control the spatial arrangement of enzyme molecules. Here, a new hierarchical shell-shell (HSS) multi-enzyme structure was constructed using interfacial growth method and colloidal hydrated zinc sulphate to be used as a soft template (Cyt C@HSS@GOx). In this process, glutathione (GSH) hybrid ZIF-67 was used as the guest MOF and hollow ZIF-8 as the host MOF. The resultant Cyt C@HSS@GOx exhibited high activity by separating cytochrome C (Cyt C) and glucose oxidase (GOx) in two independent compartments. This method can accurately locate the spatial position of multiple enzymes, weaken enzyme-to-enzyme inhibition, and enhance the channeling effect of substrates, thus improving the catalytic performance of multiple enzymes. The resulting Cyt C@HSS@GOx showed 230% higher activity than free enzymes, and maintained good stability under extreme pH and temperature conditions. Furthermore, Cyt C@HSS@GOx demonstrated 71.25% retention of its initial activity following seven cycles, providing good reusability. Particularly, Cyt C@HSS@GOx has been demonstrated to possess a glucose detection limit as low as 0.38 μmol/L, with a reaction time of only three minutes. This strategy provides a rapid and accurate glucose detection method.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250839.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<em>Bifidobacterium longum</em> DPUB-15, screened by an <em>ex vivo</em> mesenteric lymph nodes cell model, alleviates allergic responses in casein-allergic Sprague-Dawley rats","authors":"Zihao Guo, Feng Lu, Mingyang Ma, Yu Jiang, Rodríguez-Cervantes MA, Wenpu Yao, Guangqing Mu, Xuemei Zhu","doi":"10.26599/fshw.2025.9250848","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250848","url":null,"abstract":"Cow's milk allergy is a global health issue, and probiotic-based treatments may offer a promising therapeutic approach. Mesenteric lymph nodes (MLNs) play a crucial role in the development of food allergies. The present study aims to establish an <em>ex vivo</em> model based on rat-derived MLN cells to screen probiotic strains with potential to alleviate casein allergy and to investigate the effects in the casein-allergic rat model. The results showed that <em>ex vivo</em> screening <em>via</em> normal and casein-sensitized SD rat MLN cells identified three strains, with&nbsp;<em>Bifidobacterium longum</em>&nbsp;DPUB-15 selected as target strain demonstrating optimal probiotic properties. Subsequently, the present study demonstrated that both live and heat-killed&nbsp;<em>Bifidobacterium longum</em>&nbsp;DPUB-15 could alleviate casein allergy in rats. Notably, heat-killed <em>Bifidobacterium</em> <em>longum</em> DPUB-15 demonstrated superior effects, further downregulating IgG2a and histamine levels, improving ileal immune balance and colonic barrier function (ZO-1), and modulating intestinal microbiota by reducing the abundance of casein allergy-associated Lachnospirales_CAG-274 and increasing the abundance of <em>Paraprevotella</em>. In conclusion, this study provides an<em> ex vivo</em> model for screening anti-allergic probiotic strains and highlights <em>Bifidobacterium longum </em>DPUB-15 as a promising candidate for alleviating casein allergy, with heat-killed <em>Bifidobacterium</em> <em>longum</em> DPUB-15 showing potential for postbiotic development in allergy treatment.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250848.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148305572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoconfinement boosts electrochemiluminescence based on in-situ anchoring Pt NCs on mesoporous Mn ₃ O ₄ for rapid and sensitive detection of <em>salmonella typhimurium</em>","authors":"Xiaobo Zhang, Xiuwen Wang, Longjiao Zhu, Jinyan Zhu, Bing Hu, Qiuyue Zheng, Jingli Yuan, Wentao Xu, Jijuan Cao","doi":"10.26599/fshw.2025.9250845","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250845","url":null,"abstract":"The development of a simple, rapid and sensitive method for foodborne pathogens is essential to ensure public health safety. Existing methods, such as culture-based techniques and polymerase chain reaction (PCR), often face challenges in terms of time-consuming, labor-intensive, and require extensive sample preparation and amplification, which makes them impractical for rapid or on-site pathogen detection. Herein, a competitive mechanism-driven electrochemiluminescence (ECL) aptasensor was constructed for <em>Salmonella typhimurium</em> (<em>S.</em> <em>typhimurium</em>) detection without nucleic acid extraction and amplification. To boost the ECL signal of luminol-H₂O₂ systems, <em>in-situ</em> anchoring of Pt nanoclusters on mesoporous Mn₃O₄ nanoparticles (Pt NCs@M-Mn₃O₄) used as the nanoconfinement co-reactor. Benefitting from the restricted mesoporous structure, Pt NCs@M-Mn₃O₄ provided a unique microenvironment for the enrichment of H₂O₂. Furthermore, the interfacial interaction could activate the electronic transfer between active species of Pt NCs and M-Mn₃O₄, enabling a synergistic catalytic effect on ROS generation to effectively boost the ECL emission of luminol. The proposed ECL aptasensor had a linear concentration from 2 × 10¹ to 2 × 10⁶ CFU/mL with limit of detection of 8 CFU/mL of <em>S.</em> <em>typhimurium</em>. Furthermore, the method showed high anti-interference ability. Especially, satisfactory recovery values in spiked-actual samples were obtained, thereby demonstrating great potential for sensitive and reliable detection of <em>S.</em> <em>typhimurium </em>in various environments.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250845.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Proteomics-Based Reactive Metabolites and Protein Adducts: The New Perspective on the Metabolism of Food Furanocoumarins","authors":"Zhendong Wang, Shan Han, Xiaoyan Cai, Meng Li, Jiaming Qian, Qiyao Jiang, Xiqing Bian, Jian‐Lin Wu, Zhuo Yue","doi":"10.26599/fshw.2025.9250900","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250900","url":null,"abstract":"Furanocoumarins are widely found in various foods and exhibit a range of biological activities. However, due to the structural characteristics of their furan ring, they are susceptible to being metabolized by cytochrome P450 enzymes, thereby forming reactive metabolites (RMs), leading to a dual effect: firstly, the inhibition of CYP enzyme activity, which may result in food-drug interactions affecting drug absorption; and secondly, the formation of covalent bonds between RMs and proteins, potentially disrupting protein function and causing toxicity. Consequently, understanding the metabolic metabolism of furanocoumarins in biological systems is essential for ensuring food safety. This study employed chemical proteomics-based RMs and protein adducts analytical approach to investigate mechanisms of psoralen (PRN), a representative simple-structured furanocoumarin. Initially, dose-dependent hepatotoxicity was confirmed in mice treated with excessive PRN exposure. Subsequently, an LC-MS strategy was developed to identify PRN RM-protein adducts both <em>in vitro</em> (liver microsomes) and <em>in vivo</em> (mouse liver). The abundance of PRN RM-protein adducts was positively correlated with the severity of liver injury. Further analysis identified CYP2C6, CYP3A1, CYP2E1, and CYP2A3 as the primary enzymes facilitating the formation of PRN RM-protein adducts. Finally, label-free quantitative proteomics, integrated with network pharmacology, indicated that disruption of glutathione metabolism may be a potential hepatotoxic pathway activated by PRN RM-protein adducts. This study provides new insights into the dual role of furanocoumarin-induced food-drug interactions and hepatotoxicity, establishes a methodological framework for assessing food safety, and offers critical insights for their judicious application.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2025.9250900.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isocaloric high protein dietary pattern ameliorates chronic alcohol-induced liver dysfunction <em>via</em> a BDH2 involved mechanism","authors":"Jiannan Qiu, Fan Dong, Kaixin Pan, Tianchi Sun, Feiwei Cao, Qinchao Ding, Qianyu Qian, Xiaobing Dou, Qing Song, Songtao Li","doi":"10.26599/fshw.2025.9250849","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250849","url":null,"abstract":"Emerging evidence shows that the proportion of dietary macronutrients determines the pathological progression of multiple metabolic diseases, however, whether and how this affect alcohol-associated liver diseases (ALD) are largely unclear. The present study was conducted to explore the role of dietary protein proportions on ALD and to unveil its potential mechanisms. C57BL/6N mice were administrated with isocaloric Lieber-DeCarli alcohol liquid diets with different proportions of protein, including normal protein (NP, <strong>15% energy</strong>), high protein (HP, 30% energy), and low protein (LP, 10% energy), respectively. Our results showed that HP diet significantly ameliorated alcohol-induced hepatic steatosis, liver injury, oxidative stress, and inflammation, which were aggravated in LP diet-fed mice. Alcohol consumption-stimulated lipid metabolism-associated genes dysregulation was improved by HP diet. Liver transcriptome sequencing analysis and further validation identified that alcohol dramatically down-regulated 3-hydroxybutyrate dehydrogenase 2 (<em>Bdh2</em>) along with the reduction of<em> </em>β-hydroxybutyric acid, which were rescued by HP diet intervention. <em>Bdh2 </em>was also decreased in the liver of patients with ALD and alcohol-exposed hepatocytes. Spearman correlation analysis revealed that <em>Bdh2</em> was negatively associated with the indices of hepatic steatosis, liver injury, and oxidative stress. Genetically knocking-down <em>Bdh2 </em>significantly enhanced alcohol-induced hepatotoxicity, lipid accumulation, and pro-inflammatory factors expression in cultured hepatocytes. In summary, HP diet intervention alleviates chronic alcohol consumption induced liver dysfunction in mice. BDH2 is a potential novel target for ALD prevention <em>via</em> restoring the ketogenic pathway that contributes to HP diet-improved ALD. HP dietary pattern might be a promising choice for ALD management.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250849.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The improvement in stability and coloration effect of gallic acid acylation of anthocyanins and their quantum chemical mechanism","authors":"Lei Yan, Pingping Wang, 福住 一雄, Chun Chen","doi":"10.1016/j.foodw.2025.100034","DOIUrl":"https://doi.org/10.1016/j.foodw.2025.100034","url":null,"abstract":"To address the limitations in functionality and stability of current anthocyanin-based smart packaging indicators, this study developed an innovative enzymatic acylation strategy. Through lipase-catalyzed grafting of gallic acid (acylation degree: 9.16%), acylated anthocyanins with excellent thermal stability (1.5-fold extended half-life), photostability (32% higher retention after 48-hour UV irradiation), and pH sensitivity (pH 4-8) were successfully prepared. By combining characterization techniques including Fourier transform infrared spectroscopy, high-performance liquid chromatography, and ultraviolet-visible spectroscopy with density functional theory calculations, it was discovered that diacylation synergistically enhanced molecular rigidity through π-π conjugation and hydrogen bonding networks (electrostatic potential energy reduced by 1295 kJ/mol). Notably, despite the reduction in phenolic hydroxyl groups, the acylated products retained strong antioxidant activity (verified by DPPH assay) due to residual galloyl moieties. This research not only elucidated the intrinsic mechanisms of structure-property relationships but also provided novel insights for developing high-performance natural smart packaging indicators, offering important guidance for food freshness monitoring and the design of stabilized anthocyanin functional materials.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"2 1","pages":"100034-100034"},"PeriodicalIF":0.0,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and Metabolic Regulatory Effects of Ocular-Accumulative Phenolic Compounds in Dry Eye Disease Management","authors":"Ke Wang, P.P. Lee, Weipeng Li, Wenjie Wu, Christie Hang-I Lam, Ka‐Ying Wong, Man‐Sau Wong, Dennis Y. Tse, Danyue Zhao","doi":"10.26599/fshw.2025.9250836","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250836","url":null,"abstract":"Dry eye disease (DED), characterized by symptoms of dryness and inflammation in the eyes, has become a prevalent ocular disorder worldwide. While several DED medications are available, their effectiveness is often limited by the low bioavailability and rapid clearance from ocular tissues. Alternatively, phenolic compounds with ocular-targeting and anti-inflammatory properties provide viable solutions. This study aimed to explore the ability of polyphenols to reach ocular tissues and exert therapeutic effects against dry eye symptoms in <em>in vivo</em> and <em>in vitro</em> models. The therapeutic mechanisms of ocular-accumulative phenolic compounds were further investigated in a human corneal epithelial cell (HCEC) model of DED. For <em>in vivo</em> studies, db/db mice were administrated to a polyphenol-rich herbal preparation (PHP), followed by assessments of dry eye symptoms, and identification of ocular accumulative phenolic compounds through targeted metabolomics. For<em> in vitro</em> studies, HCECs were exposed to a hyperosmolar solution to induce DED, followed by evaluation of the ability of selected phenolic compounds to maintain cell viability and attenuate inflammation. For mechanistic investigation, metabolomics and lipidomics were performed on samples prepared using the innovative enhanced matrix removal (EMR)-lipid µelution technique alongside assessments on gene expression and molecular interactions through molecular docking analyses. Results show that PHP treatment significantly improved corneal surface integrity and reduced inflammation in mice. Among the phenolic metabolites detected in ocular tissues, urolithin A (UA) and 3,4-dihydroxybenzoic acid (3,4-diHBA) exhibited the strongest anti-inflammatory effects in the DED cell model, as evinced by the downregulated interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) levels. Furthermore, integrated metabolomic and lipidomic analyses revealed significant differences in multiple metabolite levels and metabolic pathways across groups. Of note, 3,4-diHBA and UA remarkably regulated amino acid metabolism, glutathione metabolism, sphingolipid and glycerophospholipid metabolic pathways, which may collectively support restoration of cellular metabolic functions related to anti-inflammation, ocular surface integrity maintenance, and osmoregulation. Overall, this study highlights the therapeutic potential of diet-derived, ocular-accumulative phenolic compounds in the management of DED.&nbsp;","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/1988847886784860162.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyvinyl alcohol-based sustainable packaging films incorporating glycerol monostearate: Fabrication, characterization, and intermolecular interactions analysis","authors":"Yiting Fan, Xinglong Xiao, Ye Zou, Jiahao Ren, Qi Zhang, Qiyue Yang, Yifang Cao, Yijia Xie, Fengsong Liu","doi":"10.1016/j.foodw.2025.100033","DOIUrl":"https://doi.org/10.1016/j.foodw.2025.100033","url":null,"abstract":"Polyvinyl alcohol (PVA) is a promising biodegradable polymer for sustainable packaging. However, its strong hydrophilicity limits practical applications. This study reports the fabrication of PVA films modified with glycerol monostearate (GMS) to address this limitation. Films containing 5%, 10%, and 15% GMS were systematically characterized by rheology, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The analyses revealed strong molecular entanglement and high compatibility between GMS and PVA, which significantly enhanced film hydrophobicity. At 15% GMS loading, the films exhibited improved water and gas barrier properties, with a water contact angle (CA) of 127.18°, a water absorption capacity (WAC) of 6.35 g/g, a water vapor transmission rate (WVTR) of 8.82 g/m 2 /d, and an oxygen transmission rate (OTR) of 58.14 (cm 3 ×cm×cm 2 ×s×Pa) -1 . Moreover, the incorporation of GMS enhanced ultraviolet (UV)-shielding performance (average UV transmittance: 75.66%) and increased tensile strength to 75.84 MPa. Molecular dynamics (MD) simulations corroborated these findings, revealing stable intermolecular hydrogen bonding that reinforced the film network. Collectively, these results underscore PVA-GMS films as high-performance, eco-friendly packaging materials with superior water resistance, barrier functionality, and mechanical strength. The hydrophilic regions of glycerol monostearate (GMS) form intermolecular hydrogen bonds with polyvinyl alcohol (PVA), while its hydrophobic regions migrate to the film's surface, effectively enhancing its water resistance. • Hydrogen bonding and molecular entanglement between PVA and GMS increased hydrophobicity. • MD simulations verified the hydrogen bonding and van der Waals forces of PVA and GMS. • The modified PVA films had promising potential in food packaging applications.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"2 1","pages":"100033-100033"},"PeriodicalIF":0.0,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalin Attenuates Chronic Pancreatitis through SIRT5-Mediated Nrf2 Deacetylation and JAK-STAT Pathway Modulation: Implications for Natural Product-Based Therapy","authors":"Hai Zhong, Bin Wu, Xiaoguang Wang, Xiaodan Yang, Lingyu Hu","doi":"10.26599/fshw.2025.9250830","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250830","url":null,"abstract":"Chronic pancreatitis (CP) is a progressive inflammatory disease marked by irreversible pancreatic fibrosis and functional decline, for which effective treatments are relatively scarce. This study explores the protective role of the dietary flavonoid baicalin (BA) in CP, with emphasis on its modulation of Sirtuin 5 (SIRT5) and associated signaling cascades. Through bioinformatics screening, nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a pivotal factor in CP pathogenesis. In rat pancreatic ductal epithelial cells (RPDECs), BA administration or Nrf2 knockdown promoted cell proliferation, inhibited apoptosis, suppressed pro-inflammatory cytokines, and facilitated Th2 polarization. In a CP rat model, BA alleviated fibrosis, reduced neutrophil accumulation, and limited collagen deposition, alongside a decline in serum levels of amylase, lipase, and trypsin. Mechanistically, multi-omics analysis demonstrated that BA activated SIRT5, leading to Nrf2 deacetylation and subsequent suppression of the JAK-STAT axis. This regulatory effect rebalanced immune responses by limiting Th1/Th17-mediated inflammation and enhancing Th2/Treg activity, thereby reducing pancreatic injury. Collectively, our findings suggest that BA mitigates CP through SIRT5/Nrf2-driven modulation of immune-fibrotic interactions, supporting its potential as a dietary agent for targeted inflammation and fibrosis control in CP.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/10.26599/FSHW.2025.9250830.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}