Folia microbiologica最新文献_第3页

Optimization of fermentation conditions for microbial transglutaminase production by Streptoverticillium cinnamoneum KKP 1658 using response surface methodology (RSM). 利用响应面法（RSM）优化肉桂链霉菌 KKP 1658 生产微生物转谷氨酰胺酶的发酵条件。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-23 DOI: 10.1007/s12223-024-01223-7

Vitaliy Kolotylo, Kamil Piwowarek, Alicja Synowiec, Marek Kieliszek

{"title":"Optimization of fermentation conditions for microbial transglutaminase production by Streptoverticillium cinnamoneum KKP 1658 using response surface methodology (RSM).","authors":"Vitaliy Kolotylo, Kamil Piwowarek, Alicja Synowiec, Marek Kieliszek","doi":"10.1007/s12223-024-01223-7","DOIUrl":"https://doi.org/10.1007/s12223-024-01223-7","url":null,"abstract":"Microbial transglutaminase (MTG) is an enzyme widely used in the food industry because it creates cross-links between proteins, enhancing the texture and stability of food products. Its unique properties make it a valuable tool for modifying the functional characteristics of proteins, significantly impacting the quality and innovation of food products. In this study, response surface methodology was employed to optimize the fermentation conditions for microbial transglutaminase production by the strain Streptoverticillium cinnamoneum KKP 1658. The effects of nitrogen dose, cultivation time, and initial pH on the activity of the produced transglutaminase were investigated. The significance of the examined factors was determined as follows: cultivation time > nitrogen dose > pH. The interaction between nitrogen dose and cultivation time was found to be crucial, having the second most significant impact on transglutaminase activity. Optimal conditions were identified as 48 h of cultivation with a 2% nitrogen source dose and an initial medium pH of approximately 6.0. Under these conditions, transglutaminase activity ranged from 4.5 to 5.5 U/mL. The results of this study demonstrated that response surface methodology is a promising approach for optimizing microbial transglutaminase production. Future applications of transglutaminase include the development of modern food products with improved texture and nutritional value, as well as its potential use in regenerative medicine for creating biomaterials and tissue scaffolds. This topic is particularly important and timely as it addresses the growing demand for innovative and sustainable solutions in the food and biomedical industries, contributing to an improved quality of life.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of cultivable endophytic fungi in a decumbent subshrub endemic of the Brazilian tropical savanna. 巴西热带稀树草原特有的外倾亚灌木中可栽培内生真菌的多样性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-19 DOI: 10.1007/s12223-024-01226-4

Jefferson Brendon Almeida Dos Reis, Jadson Diogo Pereira Bezerra, Helson Mario Martins do Vale

{"title":"Diversity of cultivable endophytic fungi in a decumbent subshrub endemic of the Brazilian tropical savanna.","authors":"Jefferson Brendon Almeida Dos Reis, Jadson Diogo Pereira Bezerra, Helson Mario Martins do Vale","doi":"10.1007/s12223-024-01226-4","DOIUrl":"10.1007/s12223-024-01226-4","url":null,"abstract":"The diversity of cultivable endophytic fungi in native subshrubs of the Brazilian Cerrado is largely unknown. This study investigated the cultivable endophytic mycobiome of stems, leaves, and flowers of Peltaea polymorpha (Malvaceae). In total, 208 endophytic fungi were isolated, 95 from stems, 65 from leaves, and 48 from flowers. The isolates were classified as ascomycetes belonging to three classes, eight orders, ten families, 12 genera, and 31 species. Diaporthe, Nigrospora, and Colletotrichum were the dominant genera in the three analyzed organs. The richness estimators suggested that the number of species might be slightly higher than observed. The highest values for the Shannon and Simpson diversity indices were observed in stems. Beta diversity showed overlapping of fungal communities in different organs, with a high rate of sharing of taxa. Furthermore, the dominant primary fungal lifestyles were plant pathogens and saprobes. Our findings show that the cultivable endophytic fungal community of P. polymorpha is species-rich and that communities in different organs share genera and species.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of gut microbiome in developing necrotizing enterocolitis. 肠道微生物组在发生坏死性小肠结肠炎中的作用。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-17 DOI: 10.1007/s12223-024-01217-5

Cevher Karaca, Hatice Aysun Mercimek Takcı

{"title":"Role of gut microbiome in developing necrotizing enterocolitis.","authors":"Cevher Karaca, Hatice Aysun Mercimek Takcı","doi":"10.1007/s12223-024-01217-5","DOIUrl":"https://doi.org/10.1007/s12223-024-01217-5","url":null,"abstract":"Necrotizing enterocolitis (NEC) is one of the most devastating intestinal diseases observed in preterm in the first days of life. Researchers have recently focused on potential predictive biomarkers for early and concomitant diagnoses. Thus, we inquired about the linkage of intestinal dysbiosis, one of the most important factors in NEC development to the gut microbiota. In this study, the systematic differences in the bacterial composition between neonates affected by NEC and healthy newborns were highlighted by metagenomic analysis. The next-generation sequencing of the V3-V4 variable region of the 16S rRNA gene and gene-specific qPCR analyzed the untargeted gut microbiota. Total bacteria, total and fecal coliform loads in stool samples with NEC were higher than control. OTU-level relative abundances of NEC infant was characterized by Firmicutes and Bacteroidetes at phylum levels. At the genus level, NEC stool was identified by the lack of Klebsiella and the presence of Roseburia, Blautia, and Parasutterella. Finally, Clostridium fessum was the predominant species of Clostridium genus in disease and healthy specimens at the species level, whereas Clostridium jeddahitimonense was at NEC diagnosis. Despite a strong relationship between pathophysiology and characterization of gut microbiota at a clinical diagnosis of NEC, our results emphasize the broad difficulty in identifying potential biomarkers.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics and in vitro properties of potential probiotic strain Fructobacillus tropaeoli KKP 3032 isolated from orange juice. 从橙汁中分离出的潜在益生菌株 Fructobacillus tropaeoli KKP 3032 的特征和体外特性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-14 DOI: 10.1007/s12223-024-01207-7

Anna Mikołajczuk-Szczyrba, Adrian Wojtczak, Marek Kieliszek, Barbara Sokołowska

{"title":"Characteristics and in vitro properties of potential probiotic strain Fructobacillus tropaeoli KKP 3032 isolated from orange juice.","authors":"Anna Mikołajczuk-Szczyrba, Adrian Wojtczak, Marek Kieliszek, Barbara Sokołowska","doi":"10.1007/s12223-024-01207-7","DOIUrl":"10.1007/s12223-024-01207-7","url":null,"abstract":"Fructobacillus, a Gram-positive, non-spore-forming, facultative anaerobic bacterium, belongs to the fructophilic lactic acid bacteria (FLAB) group. The group's name originates from fructose, the favored carbon source for its members. Fructobacillus spp. are noteworthy for their distinctive traits, captivating the interest of scientists. However, there have been relatively few publications regarding the isolation and potential utilization of these microorganisms in the industry. In recent years, F. tropaeoli has garnered interest for its promising role in the food and pharmaceutical sectors, although the availability of isolates is rather limited. A more comprehensive understanding of Fructobacillus is imperative to evaluate their functionality in the industry, given their unique and exceptional properties. Our in vitro study on Fructobacillus tropaeoli KKP 3032 confirmed its fructophilic nature and high osmotolerance. This strain thrives in a 30% sugar concentration, shows resistance to low pH and bile salts, and exhibits robust autoaggregation. Additionally, it displays significant antimicrobial activity against foodborne pathogens. Evaluating its probiotic potential, it aligns with EFSA recommendations in antibiotic resistance, except for kanamycin, to which it is resistant. Further research is necessary, but preliminary analyses confirm the high probiotic potential of F. tropaeoli KKP 3032 and its ability to thrive in the presence of high concentrations of fructose. The results indicate that the isolate F. tropaeoli KKP 3032 could potentially be used in the future as a fructophilic probiotic, protective culture, and/or active ingredient in fructose-rich food.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol potential of Botrytis fabiopsis against the weeds of agricultural fields in the Qing-Tibetan Plateau. 拟褐飞虱对青藏高原农田杂草的生物防治潜力。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-12 DOI: 10.1007/s12223-024-01225-5

Haixia Zhu, Liang Cheng

{"title":"Biocontrol potential of Botrytis fabiopsis against the weeds of agricultural fields in the Qing-Tibetan Plateau.","authors":"Haixia Zhu, Liang Cheng","doi":"10.1007/s12223-024-01225-5","DOIUrl":"https://doi.org/10.1007/s12223-024-01225-5","url":null,"abstract":"In screening biocontrol strains with broad-spectrum and high-efficiency herbicidal activities, a strain with strong pathogenicity, HY-021, was isolated from the leaves of Rumex acetosa, which was identified as Botrytis fabiopsis based on morphology and molecular biology. The herbicidal activities of the fermentation filtrate of strain HY-021 against nine weeds, including Chenopodium album L., Elsholtzia densa Benth., Malva verticillata L. var. Crispa, Polygonum lapathifolium L., Amaranthus retroflexus L., Avena fatua L., Thlaspi arvense L., Polygonum aviculare L., and Galium spurium L., were determined in vitro and in vivo. The results showed that the pathogenicity of strain HY-021 to the different weeds in vitro was as follows: E. densa > A. retroflexus > P. aviculare > P. lapathifolium > M. verticillata > T. arvense > G. spurium > A. fatua > C. album. Seven days after inoculation with the HY-021 strain, the incidences in nine weeds were in the range of 32.9-87.23%, and the disease index values of the nine weeds were 41.73-94.57%. The pathogenic effects from high to low were A. retroflexus > E. densa > A. fatua > G. spurium > C. album > M. verticillata > T. arvense > P. aviculare > P. lapathifolium. The crop safety test showed that the biocontrol strain HY-021 was safe to V. faba, P. sativum, H. vulgare, and T. aestivum, but had a slight effect on B. napus. Scanning electron microscopy showed that the mycelium of strain HY-021 invaded the tissue through the stomata of C. album leaves, parasitized and reproduced in the tissue, and gradually destroyed the tissue. The results of this study provide a basis for the development and utilization of new and efficient microbial source herbicides.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular docking and antibacterial and antibiotic-modifying activities of synthetic chalcone (2E)-1-(3'-aminophenyl)-3-(4-dimethylaminophenyl)-prop-2-en-1-one in a MepA efflux pump-expressing Staphylococcus aureus strain. 合成查尔酮(2E)-1-(3'-氨基苯基)-3-(4-二甲基氨基苯基)-丙-2-烯-1-酮在表达 MepA 外排泵的金黄色葡萄球菌菌株中的分子对接及抗菌和抗生素修饰活性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-12 DOI: 10.1007/s12223-024-01221-9

Larissa da Silva, Cicera Alane Coelho Gonçalves, Antonio Henrique Bezerra, Cristina Rodrigues Dos Santos Barbosa, Janaina Esmeraldo Rocha, Yedda Maria Lobo Soares de Matos, Lígia Cláudia Castro de Oliveira, Hélcio Silva Dos Santos, Henrique Douglas Melo Coutinho, Francisco Assis Bezerra da Cunha

{"title":"Molecular docking and antibacterial and antibiotic-modifying activities of synthetic chalcone (2E)-1-(3'-aminophenyl)-3-(4-dimethylaminophenyl)-prop-2-en-1-one in a MepA efflux pump-expressing Staphylococcus aureus strain.","authors":"Larissa da Silva, Cicera Alane Coelho Gonçalves, Antonio Henrique Bezerra, Cristina Rodrigues Dos Santos Barbosa, Janaina Esmeraldo Rocha, Yedda Maria Lobo Soares de Matos, Lígia Cláudia Castro de Oliveira, Hélcio Silva Dos Santos, Henrique Douglas Melo Coutinho, Francisco Assis Bezerra da Cunha","doi":"10.1007/s12223-024-01221-9","DOIUrl":"https://doi.org/10.1007/s12223-024-01221-9","url":null,"abstract":"Bacterial resistance has become a global concern for public health agencies. Various resistance mechanisms found in Staphylococcus aureus strains grant this bacterium resistance to a wide range of antibiotics, contributing to the rise in human mortality worldwide. Resistance mediated by efflux pumps is one of the most prevalent mechanisms in multi-resistant bacteria, which has aroused the interest of several researchers in the search for possible efflux pump inhibitors. In view of the aforementioned considerations, it is important that new strategies, such as the synthesis of chalcones, be made available as a viable strategy in antimicrobial therapy. In this study, the synthesized chalcone (2E)-1-(3'-aminophenyl)-3-(4-dimethylaminophenyl)-prop-2-en-1-one was tested for its antibacterial activity, focusing on antibiotic modification and the inhibition of the MepA efflux pump present in S. aureus strain K2068. The broth microdilution method, using microdilution plates, was employed in microbiological tests to determine the minimum inhibitory concentration of the chalcone, antibiotics, and ethidium bromide. The results show that while the chalcone did not exhibit direct antibacterial activity, it synergistically enhanced the effects of ciprofloxacin and ethidium bromide, as evidenced by the reduction in MICs. In addition, computer simulations of molecular docking demonstrate that the tested chalcone acts on the same binding site as the efflux pump inhibitor chlorpromazine, interacting with essentially the same residues. These data suggest that the chalcone may act as a MepA inhibitor.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment. 通过流式细胞仪和荧光活力评估来评价蓝藻的应激恢复能力。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-06 DOI: 10.1007/s12223-024-01212-w

Zuzana Kroupová, Eva Slaninová, Kateřina Mrázová, Vladislav Krzyžánek, Kamila Hrubanová, Ines Fritz, Stanislav Obruča

{"title":"Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.","authors":"Zuzana Kroupová, Eva Slaninová, Kateřina Mrázová, Vladislav Krzyžánek, Kamila Hrubanová, Ines Fritz, Stanislav Obruča","doi":"10.1007/s12223-024-01212-w","DOIUrl":"https://doi.org/10.1007/s12223-024-01212-w","url":null,"abstract":"Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and assessment of probiotic potential of Acidipropionibacterium acidipropionici C03B-STR from goat milk with cholesterol-lowering capability. 从具有降低胆固醇能力的山羊奶中分离酸性丙酸杆菌 C03B-STR 并评估其益生菌潜力

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-06 DOI: 10.1007/s12223-024-01222-8

Sirikhwan Tinrat, Onnicha Jiraprasertwong

{"title":"Isolation and assessment of probiotic potential of Acidipropionibacterium acidipropionici C03B-STR from goat milk with cholesterol-lowering capability.","authors":"Sirikhwan Tinrat, Onnicha Jiraprasertwong","doi":"10.1007/s12223-024-01222-8","DOIUrl":"https://doi.org/10.1007/s12223-024-01222-8","url":null,"abstract":"Elevated levels of cholesterol or triglycerides in the blood are one of the major risk factors for coronary heart disease, which is the foremost leading cause of death across the world. The aims of this study were to isolate and verify the potential probiotic strain and cholesterol-lowering capability from goat milk. The C03B-STR isolate had a broad-spectrum antibacterial property and exhibited remarkable bacteriostatic activity against Klebsiella pneumoniae ATCC 13883 (27.00 ± 1.73 mm), Pseudomonas aeruginosa ATCC 27853 (19.33 ± 0.57 mm) and Staphylococcus aureus ATCC 10832 (19.00 ± 1.00 mm) by agar well diffusion assay. This isolate is closely related to the 16S rDNA sequence of Acidipropionibacterium acidipropionici. Acidipropionibacterium acidipropionici C03B-STR can survive under acidic conditions (60.51 ± 0.02% of survival rate at pH 3.0 for 3 h) and was as bile-tolerant strain (> 80% of survival rate at 0.30% bile salts after 3 h of incubation). Furthermore, it showed significantly high cell surface hydrophobicity (74.36 ± 0.23%) and aggregation capability (> 83%) (p ≤ 0.05) but displayed low to moderate co-aggregation ability (44-61%). This strain can also be regarded as strongly adhesive (73.69 ± 0.74%) and inhibit pathogen attachment to the Caco-2 cell lines (39.20 ± 0.59-69.01 ± 0.29%). A. acidipropionici C03B-STR appeared to be safe (non-haemolytic) and controllable (sensitive to various antibiotics). Notably, it had the potential to suppress cholesterol uptake in Caco-2 cells (approximately 45%) and also reduced cholesterol level above 84% during the exponential phase under acidic conditions. Thus, A. acidipropionici C03B-STR from goat milk may be a promising novel potential cholesterol-lowering probiotic strain for application in functional foods and biotherapeutics.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A scientific research training programme for teaching biomedical students to identify the horizontal transfer of antibiotic resistance genes. 为生物医学专业学生提供科学研究培训计划，帮助他们识别抗生素抗性基因的水平转移。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-05 DOI: 10.1007/s12223-024-01219-3

Jiafang Fu, Peipei Zhang, Xunzhe Yin, Lingjia Zhu, Gongli Zong, Chuanqing Zhong, Guangxiang Cao

{"title":"A scientific research training programme for teaching biomedical students to identify the horizontal transfer of antibiotic resistance genes.","authors":"Jiafang Fu, Peipei Zhang, Xunzhe Yin, Lingjia Zhu, Gongli Zong, Chuanqing Zhong, Guangxiang Cao","doi":"10.1007/s12223-024-01219-3","DOIUrl":"https://doi.org/10.1007/s12223-024-01219-3","url":null,"abstract":"Worldwide prevalence of multi-antibiotic resistant bacteria is rapidly increasing, and the education of undergraduates and graduates about antibiotic resistance and its associated horizontal gene transfer is critical in the general effort to confront the spread of antibiotic resistance. In this study, a deeper understanding of antibiotic resistance and horizontal gene transfer was achieved by biomedical undergraduate students through a scientific research programme. The enthusiasm of students to participate in the training programme was very high, and results revealed that each student could identify the antibiotic resistance integrative and conjugative element from the Stenotrophomonas maltophilia MER1 genome. Each student could also draw the phylogenetic relationship of the antibiotic resistance integrative and conjugative element. In addition, students proved the horizontal transfer of antibiotic resistance genes from S. maltophilia MER1 to Escherichia coli strain 25DN through conjugation and PCR assays. Each group of students was able to obtain the expected results, indicating that the outcome of the scientific research programme was highly reproducible. This programme improved the theoretical knowledge about antibiotic resistance and horizontal gene transfer and the research skills of biomedical sciences students. Through this programme, students learned that antibiotic resistance genes can be horizontally transferred among different bacteria, laying a solid foundation for students to value the importance of the appropriate use of antibiotics in their future work and life.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal diversity of bacterial endophyte microbiome of mandarin (Citrus reticulata) in the northern Persian Gulf and its HCN production and N₂ fixation. 波斯湾北部柑橘（Citrus reticulata）细菌内生微生物组的时空多样性及其 HCN 生产和 N2 固定。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2024-11-05 DOI: 10.1007/s12223-024-01213-9

Tahereh Sadat Hashemi, Jalal Soltani, Davood Samsampour, Majeed Askari Seyahooei, Mostafa Ghasemi

{"title":"Spatiotemporal diversity of bacterial endophyte microbiome of mandarin (Citrus reticulata) in the northern Persian Gulf and its HCN production and N2 fixation.","authors":"Tahereh Sadat Hashemi, Jalal Soltani, Davood Samsampour, Majeed Askari Seyahooei, Mostafa Ghasemi","doi":"10.1007/s12223-024-01213-9","DOIUrl":"https://doi.org/10.1007/s12223-024-01213-9","url":null,"abstract":"Endophytes are symbionts that live in healthy plants and potentially improve the health of plant holobionts. Here, we investigated the bacterial endophyte community of Citrus reticulata grown in the northern Persian Gulf. Bacteria were isolated seasonally from healthy trees (root, stem, bark, trunk, leaf, and crown tissues) in four regions of Hormozgan province (i.e., Ahmadi, Siyahoo, Sikhoran, Roudan), a subtropical hot region in Iran. A total of 742 strains from 17 taxa, 3 phyla, and 5 orders were found, most of which belonged to Actinobacteria (Actinobacteriales) as the dominant group, followed by Firmicutes (Bacillales), Proteobacteria (Sphingomonadales, Rhizobiales), and Cyanobacteria (Synechoccales). The genera included Altererythrobacter, Arthrobacter, Bacillus, Cellulosimicrobium, Curtobacterium, Kocuria, Kytococcus, Methylopila, Mycobacterium, Nocardioides, Okiabacterium, Paracraurococcus, and Psychrobacillus. The most frequently occurring species included Psychrobacillus psychrodurans, Kytococcus schroetri, and Bacillus cereus. In addition, the overall colonization frequency and variability of endophytes were higher on the trunks. The leaves showed the lowest species variability in all sampling periods. The frequency of endophyte colonization was also higher in summer. The Shannon-Wiener (H') and Simpson indices varied with all factors, i.e., region, season, and tissue type, with the maximum in Roudan. Furthermore, 52.9% of the strains were capable of nitrogen fixation, and 70% produced antagonistic hydrogen cyanide (HCN). Thus, C. reticulata harbors a variety of bioactive bacterial endophytes that could be beneficial for host fitness in such harsh environments.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0