Folia microbiologica最新文献_第2页

Evaluation and mechanism of the antioxidant activity of lactic acid bacteria. 乳酸菌抗氧化活性的评价及机制研究。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-07 DOI: 10.1007/s12223-025-01277-1

Yuyang Xiao, Jian Yang, Xupeng Zhang, Meng Yang, Yuexiang Qin, Pinfang Huang, Dan Liu

{"title":"Evaluation and mechanism of the antioxidant activity of lactic acid bacteria.","authors":"Yuyang Xiao, Jian Yang, Xupeng Zhang, Meng Yang, Yuexiang Qin, Pinfang Huang, Dan Liu","doi":"10.1007/s12223-025-01277-1","DOIUrl":"https://doi.org/10.1007/s12223-025-01277-1","url":null,"abstract":"Lactic acid bacteria (LABs) have emerged as a significant area of study within the field of probiotics due to their diverse health benefits and wide application. This review examines the various methods used to evaluate the antioxidant activity of LABs, including in vitro chemical evaluation methods, cell model evaluation methods, and in vivo evaluation methods. Comprehensive overview of the various assessment techniques employed to elucidate the multifaceted roles of LABs in enhancing the body's natural defenses against oxidative damage. Moreover, this review emphasizes several pivotal aspects of the antioxidant effects of LABs, including the activation of the antioxidant signal pathway, the induction of antioxidative enzymes, the formation of a ROS-binding system, the production of metabolites, the enhancement of intestinal barrier integrity, the activation of the oxidative damage repair system, and the assurance of mitochondrial function. These represent the key antioxidant effects of LABs. The synthesis of this information advances our understanding of the dynamic and diverse antioxidant effects of LABs, providing a foundation for further research into their therapeutic applications in combating oxidative stress-related disorders. Future research should employ multi-omics technologies, genetic engineering, studies on synergistic effects, and large-scale clinical trials to further elucidate the molecular mechanisms underlying the antioxidant effects of LABs. This will promote their application in functional foods, pharmaceuticals, and cosmetics, providing a scientific basis for the development of more efficient antioxidant products.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease. 从山羊呼吸道疾病中分离的鼻杆菌。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-06 DOI: 10.1007/s12223-025-01279-z

Fuxiang Li, Huafeng Gao, Wenhua Zhao, Jianling Song

{"title":"Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease.","authors":"Fuxiang Li, Huafeng Gao, Wenhua Zhao, Jianling Song","doi":"10.1007/s12223-025-01279-z","DOIUrl":"https://doi.org/10.1007/s12223-025-01279-z","url":null,"abstract":"A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820T, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.0 (optimum pH 7.0), at 24-39 °C (optimum 37 °C) and with 0.5-2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131T. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006T), 37.0% (Fluviicoccus keumensis JCM 19370T), and 64.0% (Acinetobacter kanungonis JCM 34131T), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C12:0, C16:0, summed feature 3 (C16:1ω7c and/ or C16:1ω6c), C17:0, and C15:1ω6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820T represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820T (= CCTCC AB 2021475T = NBRC 115474T).","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urine-based ELISA for non-invasive diagnosis of urogenital schistosomiasis: a promising tool for resource-limited regions. 基于尿液的ELISA无创诊断泌尿生殖血吸虫病：资源有限地区的一种有前途的工具。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-02 DOI: 10.1007/s12223-025-01278-0

Tajudeen O Oriade, Kabirat A Sulaiman, Timothy Auta, Funmilayo I D Afolayan, Alexander B Odaibo, Rafaella F Q Grenfell, Ramzy G Fatem, Oyetunde T Oyeyemi

{"title":"Urine-based ELISA for non-invasive diagnosis of urogenital schistosomiasis: a promising tool for resource-limited regions.","authors":"Tajudeen O Oriade, Kabirat A Sulaiman, Timothy Auta, Funmilayo I D Afolayan, Alexander B Odaibo, Rafaella F Q Grenfell, Ramzy G Fatem, Oyetunde T Oyeyemi","doi":"10.1007/s12223-025-01278-0","DOIUrl":"https://doi.org/10.1007/s12223-025-01278-0","url":null,"abstract":"Traditional serological diagnosis can be invasive and uncomfortable, potentially discouraging patients, especially women and children, from seeking diagnosis and treatment. In resource-limited, endemic regions, non-invasive diagnostic approaches are highly desirable. This study investigated the potential of urine-based ELISA using Schistosoma haematobium soluble egg-based (Sh-SEA) and worm antigen protein (Sh-WAP) for diagnosing urogenital schistosomiasis. Urine samples were disaggregated into three groups; 50 laboratory-confirmed S. haematobium-positive individuals, 50 S. haematobium-negative individuals from the endemic area (NE), and 50 non-infected samples from a non-endemic area (NNE) were used for the ELISA immunoassay. Diagnostic performance was evaluated using receiver operating characteristic (ROC) curves, sensitivity (SS), and specificity (SP). The Sh-SEA ELISA with urine demonstrated superior diagnostic accuracy compared to Sh-WAP in both endemic (AUC = 0.89, SS = 92%, SP = 74%) and non-endemic areas (AUC = 0.77, SS = 80%, SP = 46%). Notably, both Sh-SEA and Sh-WAP antibody titers were significantly higher in infected individuals compared to the non-infected samples in both endemic and non-endemic areas (p < 0.0001). This study's findings suggest that urine-based ELISA using Sh-SEA and Sh-WAP antigens exhibits promising potential as a non-invasive diagnostic tool for urogenital schistosomiasis, particularly in resource-limited settings.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-based design of Mycobacterium tuberculosis PptT-ACP complex inhibitors using derivative design and machine learning. 基于衍生设计和机器学习的结核分枝杆菌PptT-ACP复合物抑制剂结构设计。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-02 DOI: 10.1007/s12223-025-01274-4

Badriyah Shadid Alotaibi, Vivek Dhar Dwivedi, Mohammad Amjad Kamal

{"title":"Structure-based design of Mycobacterium tuberculosis PptT-ACP complex inhibitors using derivative design and machine learning.","authors":"Badriyah Shadid Alotaibi, Vivek Dhar Dwivedi, Mohammad Amjad Kamal","doi":"10.1007/s12223-025-01274-4","DOIUrl":"https://doi.org/10.1007/s12223-025-01274-4","url":null,"abstract":"Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), remains a critical global health challenge, particularly with the rise of multidrug-resistant (MDR) strains. This study employed a comprehensive computational approach to identify and optimize inhibitors targeting the PptT-ACP complex, a key enzyme in MTB lipid biosynthesis. Virtual screening of FDA-approved compounds identified Mk3207 as a promising candidate. Stability analysis through molecular dynamics (MD) simulations validated its selection for derivative design. Three chemically tractable regions suitable for structural modification were identified, and the second region was selected for derivative design due to its favorable structural properties and binding interactions. One hundred derivatives were designed using ADMEopt and screened virtually, resulting in three top derivatives selected alongside Mk3207 for further evaluation. All compounds underwent 200 ns MD simulations in triplicate, with Compound_36 exhibiting the highest binding stability, as indicated by low root mean square deviation (RMSD) and root mean square fluctuation (RMSF) values, followed by Compound_98 and Compound_60. Free energy landscape (FEL) and principal component analysis (PCA) confirmed the thermodynamic stability of these derivatives. Predicted biological activity using machine learning (Random Forest Regression) indicated pIC50 values of 25.64, 22.43, 22.32, and 26.26 for Compound_36, Compound_98, Compound_60, and Mk3207, respectively. This study demonstrates the potential of derivative design and machine learning in designing potent MTB inhibitors, providing strong candidates for experimental validation to combat drug-resistant TB effectively.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of alkaline protease enzyme produced from marine yeast Candida orthopsilosis AKB-1 and its applications. 海洋酵母正生念珠菌 AKB-1 产碱性蛋白酶的特性及其应用。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-01 Epub Date: 2024-10-28 DOI: 10.1007/s12223-024-01216-6

Anwesha Sarkar, Anjukrishna S R, Bhaskara Rao K V

{"title":"Characterization of alkaline protease enzyme produced from marine yeast Candida orthopsilosis AKB-1 and its applications.","authors":"Anwesha Sarkar, Anjukrishna S R, Bhaskara Rao K V","doi":"10.1007/s12223-024-01216-6","DOIUrl":"10.1007/s12223-024-01216-6","url":null,"abstract":"The present study has undertaken the isolation of marine yeasts from mangrove sediment samples and their ability to produce alkaline protease enzymes. A total of 14 yeast isolates were recovered on yeast-malt agar (YMA) and yeast extract peptone dextrose (YEPD) agar medium. After screening for proteolytic activity on skim milk agar, marine yeast isolate, AKB-1 exhibited a hydrolysis zone of 18 mm. Optimal conditions for the enzyme production from yeast isolate AKB-1 were at 30 °C, pH 8, fructose as carbon source, potassium nitrate as nitrogen source, and 25% saline concentration. Under the optimal conditions, the protease enzyme activity of the isolate AKB-1 was observed to be 978 IU/mL. The structural and functional analysis was carried out through FTIR and HPLC analysis for the extracted protease enzyme. Furthermore, the enzyme produced was partially purified by solvent extraction using ethyl acetate and ammonium sulfate precipitation (3.4-fold) followed by dialysis (56.8-fold). The molecular weight of the purified enzyme was observed to be around 60 kDa using SDS-PAGE. The extracted protein showed good antibacterial activity against six different clinical bacterial pathogens and the highest against Bacillus cereus (16 ± 0.5 mm). The extracted protease enzyme was revealed to remove blood stains from cloth within 20 min of application similar to the commercial detergent. The marine yeast isolate was further identified as Candida orthopsilosis AKB-1 (Accession number KY348766) through 18S rRNA sequencing, and a phylogenetic tree was generated.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"631-643"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The next viral pandemic-where do we stand? 下一次病毒大流行——我们处于什么位置？

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-01 Epub Date: 2025-03-28 DOI: 10.1007/s12223-025-01256-6

Rui Fan, Qun Liang, Yanbo Sui, Yang Yang, Xingxing Yuan

引用次数: 0

Enhancement of carotenogenesis by Blakeslea trispora in a mixed culture with bacteria. 在与细菌混合培养的过程中，三孢叶枯藻可增强胡萝卜素的生成。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-01 Epub Date: 2024-10-03 DOI: 10.1007/s12223-024-01202-y

Maryam Azizi, Davood Zare, Abbas Akhavan Sepahi, Mehrdad Azin

{"title":"Enhancement of carotenogenesis by Blakeslea trispora in a mixed culture with bacteria.","authors":"Maryam Azizi, Davood Zare, Abbas Akhavan Sepahi, Mehrdad Azin","doi":"10.1007/s12223-024-01202-y","DOIUrl":"10.1007/s12223-024-01202-y","url":null,"abstract":"Among carotenoids, ꞵ-carotene has the highest biological activity and is found as an all-trans isomer in many biological systems. Blakeslea trispora is a microorganism that is of interest to industries for the commercial production of ꞵ-carotene. This study investigated the effect of different bacteria on carotenogenesis in B. trispora. The B. trispora bisexual mold was cultured in a production medium, and different bacterial cells were added to it after 24 h. Then, the culture conditions and the culture medium were optimized in the presence of the selected bacteria using the experimental design. The percentage of carotenoids obtained from the mixed culture was determined using high-performance liquid chromatography (HPLC). Results showed that Kocuria rhizophila had the greatest effect on increasing the production of carotenoids in B. trispora. The highest content of carotenoids obtained during optimization was 770 ± 7.5 mg/L, a 6.8-fold increase compared to the control. HPLC analysis of carotenoids indicated the presence of two main peaks, ꞵ-carotene and γ-carotene, in which the primary carotenoid was ꞵ-carotene followed by γ-carotene with a lower content. Therefore, due to the importance of ꞵ-carotene in industry, the use of biostimulants is one of the appropriate strategies to increase the production of this pigment in industry.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"545-557"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applicability of Cyclotella caspia and Thalassiosira weissflogii diatomous water growth for the removal of the antibiotic tetracycline. 鸡皮环藻和魏氏海藻硅藻水生长去除抗生素四环素的适用性。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-01 Epub Date: 2025-03-15 DOI: 10.1007/s12223-025-01255-7

Ekaterina S Solomonova, Natalia Yu Shoman, Arkady I Akimov

{"title":"Applicability of Cyclotella caspia and Thalassiosira weissflogii diatomous water growth for the removal of the antibiotic tetracycline.","authors":"Ekaterina S Solomonova, Natalia Yu Shoman, Arkady I Akimov","doi":"10.1007/s12223-025-01255-7","DOIUrl":"10.1007/s12223-025-01255-7","url":null,"abstract":"The effect of the antibiotic tetracycline on the growth and photosynthetic activity of the diatoms Cyclotella caspia Grunow, 1878, and Thalassiosira weissflogii (Grunow) G.A. Fryxell & Hasle, 1977, has been studied. The rate of tetracycline concentration decreases in the medium with and without has been estimated. The expediency and prospects of using diatoms as promising objects for water purification from tetracycline are shown. It was found that the rate and efficiency of tetracycline removal from the medium in the presence of algae depends on the initial content of the pollutant in the medium. The maximum efficiency of antibiotic removal is observed at concentrations of the pollutant provoking the hormesis growth of algae at 5 mg/L in C. caspia and 10 mg/L in T. weissflogii. In samples with C. caspia and tetracycline, the residual antibiotic content in the medium was 10-14% lower than in samples without algae. In the experiment with T. weissflogii, this indicator reached 15-16%. At concentrations of the pollutant, 2 and 10 mg/L in C. caspia and 2 and 5 mg/L in T. weissflogii, the effectiveness of removing the antibiotic by algae was lower. The residual content of tetracycline in the medium was on average 8-11% lower than in samples without algae. At antibiotic concentrations of 15 and 20 mg/L, the rate of its removal in samples with and without algae did not significantly differ. This result is obviously due to the toxic effect of tetracycline on cells, leading to pronounced inhibition of algae growth and/or death.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"657-663"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio sulfur granules developed with Methylobacterium thiocyanatum promoted black gram growth and yield in sulfur-deficient calcareous vertisol. 利用硫氰酸甲基杆菌开发的生物硫颗粒促进了缺硫钙质蛭石中黑禾的生长和产量。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-01 Epub Date: 2024-10-05 DOI: 10.1007/s12223-024-01199-4

G Akila, S Thiyageshwari, R Krishnamoorthy, D Selvi, A Manikandan, R Anandham, M Djanaguirman

{"title":"Bio sulfur granules developed with Methylobacterium thiocyanatum promoted black gram growth and yield in sulfur-deficient calcareous vertisol.","authors":"G Akila, S Thiyageshwari, R Krishnamoorthy, D Selvi, A Manikandan, R Anandham, M Djanaguirman","doi":"10.1007/s12223-024-01199-4","DOIUrl":"10.1007/s12223-024-01199-4","url":null,"abstract":"High soil pH and excess CaCO3 are major contributors to calcareous soil limitations on crops' access to essential nutrients, especially phosphorus (P) and micronutrients, which in turn impact pulses yields and growth. The purpose of this study was to determine the effect of bio sulfur granules (BSG) on the growth of black gram and the availability of nutrients in calcareous vertisols deficient in sulfur. BSG was developed by using sulfur-oxidizing bacteria (SOB) and elemental sulfur (ES) through an incubation study. Developed BSG was tested in a pot and field conditions to evaluate their effectiveness on black gram growth and yield. In the incubation study, soil treated with Methylobacterium thiocyanatum VRI7-A4 and ES (40 kg S/ha) significantly decreased pH and increased available S (SO42-) in calcareous soils. After 40 days of incubation, the solubility of P, Fe, and Zn was greatly increased by the addition of ES @ 40 kg S/ ha in combination with M. thiocyanatum VRI7-A4 or Pandoraea thiooxydans ATSB16. Black gram in S-deficient calcareous soil was improved by the application of BSG (ES @ 40 kg S/ ha with M. thiocyanatum VRI7-A7) in terms of root and shoot lengths, nodule number, plant biomass, pod yield, and biological yield as compared to control. The same treatment greatly increased plant nutrient intake as well as the concentrations of P, Fe, and Zn in the soil. The results showed that the addition of BSG granules (ES @ 40 kg S/ha + M. thiocyanatum VRI7-A4) to calcareous vertisol deficient in S enhanced the nutrient solubility through S oxidation. The developed bio sulfur granules may be added to the fertilizer schedule of the pulses growers to get improved crop growth and yield of black gram in calcareous soil.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"559-574"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of rhizosphere soil microbial communities and environmental factors on growth and the active ingredients of Angelica sinensis in Gansu Province, China. 根圈土壤微生物群落和环境因子对甘肃省当归生长及有效成分的影响

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-06-01 Epub Date: 2024-10-29 DOI: 10.1007/s12223-024-01210-y

Zuhua Yan, Hui Jin, Xiaoyan Yang, Deng Min, Xinxin Xu, Cuiping Hua, Bo Qin

{"title":"Effect of rhizosphere soil microbial communities and environmental factors on growth and the active ingredients of Angelica sinensis in Gansu Province, China.","authors":"Zuhua Yan, Hui Jin, Xiaoyan Yang, Deng Min, Xinxin Xu, Cuiping Hua, Bo Qin","doi":"10.1007/s12223-024-01210-y","DOIUrl":"10.1007/s12223-024-01210-y","url":null,"abstract":"The growth and accumulation of active ingredients of Angelica sinensis were affected by rhizosphere soil microbial communities and soil environmental factors. However, the correlationship between growth and active ingredients and soil biotic and abiotic factors is still unclear. This study explored rhizosphere soil microbial community structures, soil physicochemical properties, enzyme activities, and their effects on the growth and active ingredient contents of A. sinensis in three principal cropping areas. Results indicated that the growth indices, ligustilide, ferulic acid contents, and soil environmental factors varied in cropping areas. Pearson correlation analysis revealed that the growth of A. sinensis was affected by organic matter, total nitrogen, total phosphorus, and available phosphorus; ferulic acid and ligustilide accumulation were related to soil catalase and alkaline phosphatase activities, respectively. Illumina MiSeq sequencing showed that the genera Mortierella and Conocybe were the dominant fungal communities, and Sphingomonas, Pseudomonas, Bryobacter, and Lysobacter were the main bacterial communities associated with the rhizosphere soil. Kruskal-Wallis one-way ANOVA and Spearman correlation conjoint analysis demonstrated a significant positive correlation (p < 0.001) among the composition of the rhizosphere microbial communities at all three sampling sites. The growth and active ingredient accumulation of A. sinensis not only was significantly susceptible to the bacterial communities of Sphingomonas, Epicoccum, Marivita, Muribaculum, and Gemmatimonas but also were significantly influenced by the fungal communities of Inocybe, Septoria, Tetracladium, and Mortierella (p < 0.05). Our findings provide a scientific basis for understanding the relationship between the growth and active ingredients in A. sinensis and their corresponding rhizosphere soil microbial communities, soil physicochemical properties, and enzyme activities.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":"673-687"},"PeriodicalIF":2.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0