Folia microbiologica最新文献_第2页

Pichia Toolkit: Use of the combinatorial library screening system for expression of a marine laccase. 毕赤酵母工具包：使用组合文库筛选系统表达海洋漆酶。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-24 DOI: 10.1007/s12223-025-01276-2

Igor Vinicius Ramos Otero, Magdalena Haslbeck, Volker Sieber, Lara Durães Sette

{"title":"Pichia Toolkit: Use of the combinatorial library screening system for expression of a marine laccase.","authors":"Igor Vinicius Ramos Otero, Magdalena Haslbeck, Volker Sieber, Lara Durães Sette","doi":"10.1007/s12223-025-01276-2","DOIUrl":"https://doi.org/10.1007/s12223-025-01276-2","url":null,"abstract":"Pnh_Lac1 (Lac1) gene from the marine-derived fungus Peniophora sp. CBMAI 1063 was expressed in Pichia pastoris using the Pichia Toolkit system. Constitutive (pGAP, pPET9, pG1, pG6, and pADH2) and methanol-inducible (pAOX1, pDAS1, and pPMP20) promoters were assessed in combination with 21 different signal peptides and His-tag about efficiency in laccase production. Next, 3,200 variants were screened, different culture conditions were evaluated, and an investigation was performed in a bench-scale bioreactor for the best variant selected. The influence of promoters and signal peptides on Lac1 expression was demonstrated in the constitutive libraries. The change from pG6 to pGAP resulted in a 171-fold increase in production. Changing the alpha-mating factor peptide by the native signal peptide of the Lac1 gene decreased laccase production 22-fold. The promoters pGAP (constitutive library) and pAOX1 (inductive library) performed best. The association with the signal peptide αAmylase-αMFD was more efficient for both promoters. The constitutive expression of Lac1 had a 1.37-fold greater production compared to the inducible expression achieved by pAOX1 and was considered more suitable for laccase expression. Culturing the best producer variant pGAP_αA1 at pH 6 and 18 °C resulted in the best production rate in deep-well plates (90 U/L). Constitutive laccase production in a 2-L bioreactor resulted in a peak production of 178 U/L after 78 h. Pichia Toolkit was efficient in the selection of the best molecular regulation and secretion of Lac1. Our findings contribute to the development of marine biotechnology and will serve as the basis for Lac1 production optimization.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight on long non-coding RNA expression profile in THP-derived macrophages infected by Mycobacterium tuberculosis H37Rv, H37Ra, and BCG. 结核分枝杆菌H37Rv、H37Ra和BCG感染的thp源性巨噬细胞中长链非编码RNA表达谱的研究

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-16 DOI: 10.1007/s12223-025-01272-6

Shima Hadifar, Abozar Ghorbani

{"title":"Insight on long non-coding RNA expression profile in THP-derived macrophages infected by Mycobacterium tuberculosis H37Rv, H37Ra, and BCG.","authors":"Shima Hadifar, Abozar Ghorbani","doi":"10.1007/s12223-025-01272-6","DOIUrl":"https://doi.org/10.1007/s12223-025-01272-6","url":null,"abstract":"Emerging evidence has suggested a potential role for long non-coding RNAs (lncRNAs) in transcriptome dysregulation during Mycobacterium tuberculosis (Mtb) infection. Understanding the regulatory functions of lncRNAs can provide further insight into the interaction between Mtb and the host. In this study, we sought to explore the lncRNA signature in the Mtb-infected THP1 macrophages (H37Rv, H37Ra, and BCG strains) using the publicly available RNA sequencing dataset of GSE162729. Our analysis identified 6202 putative lncRNAs, with the majority being novel lncRNAs, indicating their significant involvement in the Mtb-infected macrophages. We also identified several differentially expressed lncRNA genes specifically induced in each infected group. Reactome enrichment pathway analysis on cis target genes of lncRNAs revealed that inflammatory immune responses were the predominant features of lncRNAs induced during the H37Rv infection compared to H3Ra and BCG infection. Scavenging by class A receptors and inflammasomes were also highlighted as the common enriched terms among Mtb- and BCG-infected groups. Moreover, we highlighted several potential lncRNAs as hub genes in the predicted regulatory network between the differentially expressed lncRNAs and miRNAs in Mtb-infected THP-1 cells. These findings suggested a possible diverse regulatory role for lncRNAs in the macrophage response to different Mycobacterium strain infections. Further functional study of the lncRNA genes in Mtb infection, while considering the genetic background of the Mtb strain, will be a promising focus for future research.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological activities of citrus fruit-derived copper oxide nanoparticles: towards sustainable antimicrobial and antioxidant solutions. 柑橘类水果衍生的氧化铜纳米颗粒的生物活性：朝着可持续的抗菌和抗氧化解决方案。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-10 DOI: 10.1007/s12223-025-01266-4

Bhanu Krishan, Anu Kumar, Wamik Azmi, Sunny Dhiman

{"title":"Biological activities of citrus fruit-derived copper oxide nanoparticles: towards sustainable antimicrobial and antioxidant solutions.","authors":"Bhanu Krishan, Anu Kumar, Wamik Azmi, Sunny Dhiman","doi":"10.1007/s12223-025-01266-4","DOIUrl":"https://doi.org/10.1007/s12223-025-01266-4","url":null,"abstract":"The synthesis of CuO NPs from Citrus fruit peel waste is a noteworthy strategy for the effective repurposing utilization of waste and its application in therapeutic studies. Synthesized copper oxide nanoparticles (CuO NPs) from citrus fruit extracts displayed a dark greenish-black colour with sizes ranging from 379.41, 113.19 and 142.76 nm of lemon, orange and tangerine CuO NPs. Phytochemical screening confirmed the presence of phytochemicals in the extracts wherein lemon CuO NPs lacked flavonoids and cardiac glycosides, while orange CuO NPs lacked alkaloids and flavonoids, and tangerine CuO NPs lacked only alkaloids. The decrease in phenolic concentration in CuO NPs was attributed to complex formation with metal ions. Tangerine CuO NPs exhibited the highest antioxidant activity, while lemon CuO NPs showed the highest total antioxidant capacity. Antibacterial activity increased with CuO NP concentration, with tangerine CuO NPs displaying the highest activity against both Bacillus subtilis subtilis strain 168 and Escherichia coli strain PU-1 isolated from Ghagghar river, Haryana, India. This activity was linked to the disruption of bacterial cell membranes and oxidative stress, supported by the interaction between CuO NPs and bacterial cell components. These findings contribute to understanding of various potential applications of citrus fruit-derived CuO NPs in antimicrobial and antioxidant therapies.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actinobacillus seminis DnaK interacts with bovine transferrin, lactoferrin, and hemoglobin as a putative iron acquisition mechanism. 半放线杆菌DnaK与牛转铁蛋白、乳铁蛋白和血红蛋白相互作用，作为一种假定的铁获取机制。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-10 DOI: 10.1007/s12223-025-01271-7

Candelario Vazquez-Cruz, Edmundo Reyes-Malpica, J Fernando Montes-García, Pamela Bautista-Betancourt, Elena Cobos-Justo, Miguel A Avalos-Rangel, Erasmo Negrete-Abascal

{"title":"Actinobacillus seminis DnaK interacts with bovine transferrin, lactoferrin, and hemoglobin as a putative iron acquisition mechanism.","authors":"Candelario Vazquez-Cruz, Edmundo Reyes-Malpica, J Fernando Montes-García, Pamela Bautista-Betancourt, Elena Cobos-Justo, Miguel A Avalos-Rangel, Erasmo Negrete-Abascal","doi":"10.1007/s12223-025-01271-7","DOIUrl":"https://doi.org/10.1007/s12223-025-01271-7","url":null,"abstract":"Ovine epididymitis, caused by Actinobacilus seminis, is an infectious disease that produces atrophy of the testis, low fertility, and sterility in infected animals. Iron is a microelement necessary for different vital functions in all organisms and most microorganisms. A. seminis iron acquisition mechanisms are undiscovered. For this reason, this work aimed to know the mechanisms this bacterium possesses to respond when grown in an iron restriction culture medium. A. seminis up-expressed three proteins, including a transferrin binding protein, and down-expressed seven (enzymes and putative adhesins) proteins when grown with 2,2'dipyridyl. With chelate, its growth was reduced by 40%, but it was recovered by adding 50-µM FeCl3. No siderophore production was detected with the CAS-BHI medium assay, but siderophore transporter proteins are present. Under normal growth conditions, this microorganism expresses a protein of 70 kDa, identified by mass spectrometry as DnaK. A. seminis DnaK interacts with biotin-labeled transferrin, lactoferrin, or bovine hemoglobin but not with biotin-labeled apo-transferrin or apo-lactoferrin, suggesting its participation in iron acquisition. This protein diminished its expression in iron restriction conditions at 37 °C but remained unchanged at 40 °C, and it is immune recognized by sheep serum with epididymitis. These different iron acquisition mechanisms could give rise to A. seminis, infecting different host tissues.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation of fosfomycin resistance genes in Escherichia coli isolates. 大肠杆菌中磷霉素耐药基因的分子特征分析。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-07 DOI: 10.1007/s12223-025-01269-1

Funda Yag, Fikriye Milletli Sezgin, Elif Sevim

{"title":"Molecular characterisation of fosfomycin resistance genes in Escherichia coli isolates.","authors":"Funda Yag, Fikriye Milletli Sezgin, Elif Sevim","doi":"10.1007/s12223-025-01269-1","DOIUrl":"https://doi.org/10.1007/s12223-025-01269-1","url":null,"abstract":"Fosfomycin is a well-known antibiotic that exhibits broad-spectrum activity against various bacterial pathogens, including gram-negative strains and some gram-positive strains such as staphylococci. The use of parenteral fosfomycin has been recently revised because the antibiotic has been found to effectively manage serious infections caused by multidrug-resistant pathogens. The occurrence of fosfomycin resistance could threaten the reintroduction of this antibiotic for the treatment of bacterial infections. In this study, a total of 24 fosfomycin-resistant Escherichia coli isolates obtained from urine samples were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes. The replication origins of the conjugative and transformant plasmids obtained from the isolates were examined using the replication origin determination method based on the polymerase chain reaction (PCR). Through the PCR process performed with the fosA, fosA3, fosB, fosC, fosC2, and fosX genes to determine fosfomycin resistance, one out of 24 samples was found to be fosA3 gene-positive. A Class-1 integron gene was detected in three fosfomycin-resistant E. coli isolates, while no Class-2 integrons were detected in any isolate. The conjugation experiments demonstrated that the fosA3 gene was transferable in one isolate that also carried the blaTEM, blaCTX-M-15, and aac(6')-ib-cr genes. Through plasmid isolation in the transconjugant E. coli isolates, it was determined that the E. coli isolate FF21 carried fosfomycin resistance on the plasmid. To ensure the continued effective use of fosfomycin as a treatment option, fosfomycin resistance needs to be detected and closely monitored. Given the global rise in plasmid-transmissible genes, we anticipate a growing resistance to fosfomycin in the near future.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of native Bacillus thuringiensis strains possessing nematicidal specific cry genes against Meloidogyne incognita. 原生苏云金芽孢杆菌对丝虫病的特异性杀线虫基因评价。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-06 DOI: 10.1007/s12223-025-01268-2

Paramjeet, Devendra Jain, Chandra Prakash Nama, Santosh Ranjan Mohanty

{"title":"Evaluation of native Bacillus thuringiensis strains possessing nematicidal specific cry genes against Meloidogyne incognita.","authors":"Paramjeet, Devendra Jain, Chandra Prakash Nama, Santosh Ranjan Mohanty","doi":"10.1007/s12223-025-01268-2","DOIUrl":"https://doi.org/10.1007/s12223-025-01268-2","url":null,"abstract":"Plant-parasitic nematodes, including root-knot nematodes, are phyto-parasites that cause significant crop damage and economic losses. Bacillus thuringiensis (Bt), which produces nematicidal toxins, is extensively used to combat nematode infestations in agricultural and horticultural crops. This research assessed the efficacy of native Bt strains as a biocontrol agents against the root-knot nematode Meloidogyne incognita. Twenty native Bt strains were evaluated for the presence of nematicidal cry genes using PCR. Eight strains, namely Bt1, Bt5, Bt6, Bt7, Bt17, Bt19, Bt23, and Bt24, exhibited the presence of nematicidal cry genes, specifically cry5, app6, cry12, cry13, cry14, cry21, xpp55, cry31, cry73, and cry40, as determined by gene-specific primers. The in vitro effectiveness of the Bt strains was assessed against M. incognita using a cavity block test, revealing that the Bt strains, namely Bt7 and Bt19, impeded the hatching of M. incognita eggs and were deadly to nematode larvae (J2 stage). SEM analysis of spore-crystal mixtures of Bt isolates revealed different crystal shapes that confirmed the nematicidal activity. Pot experiments revealed that the Bt7 and Bt19 strains are the most efficacious biological agents, exhibiting superior nematicidal activity in Brinjal and Tomato. The molecular characterization of the most virulent Bt strains, namely Bt-7 and Bt-19, using 16S rDNA sequencing, validated their molecular identification as Bacillus thuringiensis.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and activity enhancement of extracellular tyrosinase from a protease-silenced zygomycete Amylomyces rouxii strain. 一株蛋白酶沉默的粘连菌rouxiamylomyces胞外酪氨酸酶的纯化及活性增强。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-05-02 DOI: 10.1007/s12223-025-01264-6

Jaime Marcial-Quino, Francisco J Fernández, Francisco Fierro, Alba M Montiel-González, Araceli Tomasini

{"title":"Purification and activity enhancement of extracellular tyrosinase from a protease-silenced zygomycete Amylomyces rouxii strain.","authors":"Jaime Marcial-Quino, Francisco J Fernández, Francisco Fierro, Alba M Montiel-González, Araceli Tomasini","doi":"10.1007/s12223-025-01264-6","DOIUrl":"https://doi.org/10.1007/s12223-025-01264-6","url":null,"abstract":"The intra- and extra-cellular monophenolase and diphenolase activities of the tyrosinase produced by Amylomyces rouxii were determined in submerged culture using Melin-Norkrans medium supplemented with 12.5 mg/L pentachlorophenol (PCP) and 0.1 g/L tyrosine. Maximal intracellular monophenolase activity was 180 U/mL while maximal extracellular monophenolase activity was 80 U/mL, both using p-cresol as substrate. For diphenolase, the highest intracellular activity was 2233 U/mL using 4-tert-butylcatechol (TBC) as substrate and extracellular diphenolase activity was 975 U/mL with catechol as substrate. The peak tyrosinase activity (mono- and diphenolase) was observed at 48 h of culture. The transformant A412-3 exhibited the highest extracellular activities, with a 2.14-fold increase in monophenolase and a 3.02-fold increase in diphenolase activity compared to the parental strain of A. rouxii. Additionally, it was confirmed that the enzyme secreted was in its active form. Extracellular tyrosinase from the transformant A412-3 was partially purified, achieving a purification factor of 10.6. SDS-PAGE analysis of partially purified tyrosinase revealed three bands of 40, 53, and 130 kDa. These bands were sequenced by LC-MS/MS, revealing eight peptides that showed similarity to tyrosinases from different fungi. It was determined that purified tyrosinase exhibited higher diphenolase activity than monophenolase activity, in line with previous studies on fungal tyrosinases.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The response surface method enables efficient optimization of induction parameters for the production of bioactive peptides in fed-batch bioreactors using Escherichia coli. 响应面法能够有效优化利用大肠杆菌进料间歇式生物反应器生产生物活性肽的诱导参数。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-26 DOI: 10.1007/s12223-025-01265-5

Z R Khasanshina, I A Kornakov, E A Buslaeva, R V Drai

{"title":"The response surface method enables efficient optimization of induction parameters for the production of bioactive peptides in fed-batch bioreactors using Escherichia coli.","authors":"Z R Khasanshina, I A Kornakov, E A Buslaeva, R V Drai","doi":"10.1007/s12223-025-01265-5","DOIUrl":"https://doi.org/10.1007/s12223-025-01265-5","url":null,"abstract":"The production of recombinant peptides is critical in biotechnology and medicine for treating a variety of diseases. Thus, there is an urgent need for the development of quick, scalable, and cost-effective recombinant protein expression strategies. This study optimizes induction conditions for an insulin precursor, an analog GLP-1 precursor, and a peptide for COVID-19 therapy expression in E. coli using the response surface method. Factors such as pH, temperature, induction time, isopropyl-β-D-thiogalactopyranoside concentration, and optical density significantly influence peptide productivity. Experimental validation supports the effectiveness of these models in predicting peptide yields under optimal conditions. The optimal induction conditions were determined as follows: temperature at 37 °C, pH of the medium 7.0-8.0, induction at the early logarithmic phase of growth, isopropyl-β-D-thiogalactopyranoside concentration of 0.05 mM, and induction time of 6 h. After model validation, the productivity of each peptide producer exceeded 3 g/L. The optimal conditions achieved peptide titers significantly higher than those previously reported, suggesting that this technique is a versatile cultivation technology for the efficient production of different recombinant peptides. In conclusion, our research enhances the understanding of how tailored cultivation conditions can optimize recombinant peptide production efficiency.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of Lactobacillus reuteri strain I300 against Helicobacter pylori adhesion, invasion, and inflammatory response in gastric epithelial cells in vitro. 罗伊氏乳杆菌I300菌株对幽门螺杆菌粘附、侵袭和胃上皮细胞炎症反应的体外抑制作用

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-17 DOI: 10.1007/s12223-025-01263-7

Ghazaleh Talebi, Ali Nabavi-Rad, Zahra Sadeghloo, Michael Doulberis, Mohammad Reza Zali, Abbas Yadegar

{"title":"Inhibitory effects of Lactobacillus reuteri strain I300 against Helicobacter pylori adhesion, invasion, and inflammatory response in gastric epithelial cells in vitro.","authors":"Ghazaleh Talebi, Ali Nabavi-Rad, Zahra Sadeghloo, Michael Doulberis, Mohammad Reza Zali, Abbas Yadegar","doi":"10.1007/s12223-025-01263-7","DOIUrl":"https://doi.org/10.1007/s12223-025-01263-7","url":null,"abstract":"The increasing rate of Helicobacter pylori (H. pylori) antibiotic resistance has attenuated the effectiveness of conventional antibiotic-based treatment regimens. This study was aimed at investigating the in vitro inhibitory effects of Lactobacillus reuteri (L. reuteri) strain I300 against H. pylori. The inhibitory effects of live L. reuteri I300 and its different formulations I300L, I300G, and I300T were examined on H. pylori adhesion and invasion to AGS cells. Auto-aggregation and co-aggregation assays and also scanning electron microscopy were performed, evaluating L. reuteri capacity to auto-aggregate and co-aggregate with H. pylori. RT-qPCR and ELISA were used to investigate the expression, and production level of inflammation-related cytokines TNF-α, IL-8, and IL-10. E-cadherin expression level was also measured, determining L. reuteri potential effect on AGS cells integrity. L. reuteri presented a time-dependent capacity to auto-aggregate and co-aggregate with H. pylori. Live L. reuteri and its formulations significantly reduced H. pylori adhesion and invasion of AGS cells. H. pylori treatment with L. reuteri reduced proinflammatory cytokines TNF-α and IL-8 production while increasing anti-inflammatory cytokine IL-10 production. L. reuteri promoted the epithelial cell-cell contact by upregulating E-cadherin expression. This study indicated L. reuteri I300 as a potential probiotic strain with co-aggregation capacity and inhibitory effects against H. pylori adhesion, invasion, and inflammation.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between the skin microbiome and probiotics in the healing of burn injuries. 皮肤微生物群与益生菌在烧伤愈合中的关系。

IF 2.4 4区生物学

Folia microbiologica Pub Date : 2025-04-14 DOI: 10.1007/s12223-025-01262-8

Samane Teymouri, Maryam Pourhajibagher, Abbas Bahador

{"title":"The relationship between the skin microbiome and probiotics in the healing of burn injuries.","authors":"Samane Teymouri, Maryam Pourhajibagher, Abbas Bahador","doi":"10.1007/s12223-025-01262-8","DOIUrl":"https://doi.org/10.1007/s12223-025-01262-8","url":null,"abstract":"The relationship between the skin microbiome and probiotics in the healing of burn injuries has garnered significant attention in recent years. Burn injuries disrupt the delicate balance of the skin microbiome, leading to complications in the healing process. Probiotic therapies have emerged as promising interventions to restore microbial balance, inhibit biofilm formation, and accelerate tissue repair. Probiotics may also mitigate the risk of antibiotic-resistant infections, which is a major concern in burn units. By enhancing immune responses and stimulating the production of antimicrobial peptides, probiotics can effectively combat bacterial colonization and prevent the emergence of drug-resistant strains. A combination of probiotics with other therapies, such as phages or nanoparticles, holds significant promise for enhancing burn healing. This approach can effectively treat burn wounds by promoting wound healing synergy, preventing infection, modulating the immune response, and disrupting biofilms. Overall, the relationship between the skin microbiome and probiotics in burn wound healing has substantial potential to advance the field of burn wound management.","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0