{"title":"基于超像素的根显微图像分割方法在真菌定植率估计中的应用。","authors":"Laurie Paulin, Didier Techer","doi":"10.1007/s12223-025-01292-2","DOIUrl":null,"url":null,"abstract":"<p><p>This work illustrates a novel application of a supervised superpixel-based segmentation method for root micrograph classification and total fungal colonization rate estimation. Two procedures relying on successive classifier application on different root micrographs or on the same micrograph but with an increasing number of labels to be assigned to each picture element category are compared to a reference grid-intersect count method. Finally, supervised classification with at least 16 labels on the same picture appears as a convenient method for obtaining rapid and confident colonization rate estimates. We suggest this kind of method may be easily and routinely implemented for research or educational purposes.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of a superpixel-based segmentation method to root micrographs for total fungal colonization rate estimation.\",\"authors\":\"Laurie Paulin, Didier Techer\",\"doi\":\"10.1007/s12223-025-01292-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This work illustrates a novel application of a supervised superpixel-based segmentation method for root micrograph classification and total fungal colonization rate estimation. Two procedures relying on successive classifier application on different root micrographs or on the same micrograph but with an increasing number of labels to be assigned to each picture element category are compared to a reference grid-intersect count method. Finally, supervised classification with at least 16 labels on the same picture appears as a convenient method for obtaining rapid and confident colonization rate estimates. We suggest this kind of method may be easily and routinely implemented for research or educational purposes.</p>\",\"PeriodicalId\":12346,\"journal\":{\"name\":\"Folia microbiologica\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia microbiologica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12223-025-01292-2\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia microbiologica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12223-025-01292-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Application of a superpixel-based segmentation method to root micrographs for total fungal colonization rate estimation.
This work illustrates a novel application of a supervised superpixel-based segmentation method for root micrograph classification and total fungal colonization rate estimation. Two procedures relying on successive classifier application on different root micrographs or on the same micrograph but with an increasing number of labels to be assigned to each picture element category are compared to a reference grid-intersect count method. Finally, supervised classification with at least 16 labels on the same picture appears as a convenient method for obtaining rapid and confident colonization rate estimates. We suggest this kind of method may be easily and routinely implemented for research or educational purposes.
期刊介绍:
Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.