Folia microbiologica最新文献

{"title":"Nanobodies: a new frontier in influenza virus neutralization.","authors":"Mohamed J Saadh, Waleed K Abdulsahib, Ashok Kumar Bishoyi, Suhas Ballal, Abhayveer Singh, Suman Saini, Khasankhodja Abidov, Kamal Kant Joshi, Munther Kadheem, Manizhe Jozpanahi, Mohammad Darvishi","doi":"10.1007/s12223-025-01303-2","DOIUrl":"https://doi.org/10.1007/s12223-025-01303-2","url":null,"abstract":"<p><p>Recurrent epidemics and pandemics caused by seasonal human influenza viruses result in substantial morbidity and are a significant public health burden worldwide annually. Antiviral drugs are used to treat influenza infections but have several limitations.. Therefore, monoclonal antibody therapy is an exciting and promising approach. Nanobodies, also known as single-domain antibodies, are a new class derived from heavy-chain-only antibodies found in camelids like alpacas, llamas, and camels. These antibodies neutralize influenza viruses by targeting various proteins through multiple mechanisms. For example, they can target the hemagglutinin protein to prevent its functions. By focusing on conserved epitopes, they can neutralize a variety of influenza subtypes, including seasonal flu strains and possible pandemic variants. Additionally, these antibodies can neutralize free-floating viruses in the extracellular environment, preventing them from infecting cells. They can reduce the viral load and limit the spread of the infection. Using nanobodies to neutralize influenza viruses provides numerous advantages compared to conventional antibodies. Thanks to their unique properties, nanobodies play a crucial role in fighting influenza, improving disease management, and strengthening public health responses. In this review, we summarize the role of nanobodies in influenza virus neutralization.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare and first report of Ochrobactrum intermedium co-infection in coxsackievirus-A24-infected acute hemorrhagic conjunctivitis patients.","authors":"Sthita Pragnya Behera, Pooja Bhardwaj, Prashansha Srivastava, Shashikant Tiwari, Manoj Kumar, Nalini Mishra, Ritesh Kumar, Nirbhay Singh, Rajeev Singh, Hari Shanker Joshi, Gaurav Raj Dwivedi","doi":"10.1007/s12223-025-01300-5","DOIUrl":"https://doi.org/10.1007/s12223-025-01300-5","url":null,"abstract":"<p><p>Epidemic form of acute hemorrhagic conjunctivitis was reported from different geographical locations of the world, during 2023. Since the viral agents are well established behind acute hemorrhagic conjunctivitis outbreaks, this study aims to investigate the bacterial agent associated with the acute hemorrhagic conjunctivitis outbreak that occurred in the eastern Uttar Pradesh region of India. The bacterial infection was investigated in 91 conjunctival swabs collected from acute hemorrhagic conjunctivitis patients during the outbreak. Total nucleic acid was extracted from the ocular swab collected from acute hemorrhagic conjunctivitis patients, followed by the detection of human adenovirus and pan-enterovirus using PCR. Further, the isolation of bacteria was performed using these clinical samples. Characterization of the bacterial isolates was done using the VITEK-2 system and 16S ribosomal RNA sequencing. Of 64 conjunctival swabs positive for coxsackievirus-A24 samples, two clinical specimens showed bacterial growth. Both isolates were identified as Ochrobactrum anthropi via VITEK-2 with 93% and 95% confidence levels. While 16S ribosomal RNA analysis characterized the isolates as Ochrobactrum intermedium. Ochrobactrum intermedium is an emerging multidrug-resistant bacterium and is reported to cause a variety of clinical infections. This study first reported the Ochrobactrum intermedium infection in two coxsackievirus-A24 infected acute hemorrhagic conjunctivitis patients.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing analysis of Candida glabrata isolates collected from patients with selected drug-resistant candidiasis hospitalized in Eastern Poland.","authors":"Sebastian Kubica, Weronika Szulińska, Celina Kruszniewska-Rajs, Magdalena Kimsa-Dudek, Alina Olender, Agnieszka Bogut, Magdalena Szukała, Wojciech Dąbrowski, Daniel Pietrzak, Mariusz Gagoś, Joanna Magdalena Gola","doi":"10.1007/s12223-025-01298-w","DOIUrl":"https://doi.org/10.1007/s12223-025-01298-w","url":null,"abstract":"<p><p>The epidemiology data for candidiasis indicate an increase in Candida glabrata infections. Moreover, several reports have shown an increasing number of drug-resistant cases of these infections. The source of drug resistance can often be traced to genetic mutations in genes related to a drug's mechanism of action. Therefore, we conducted whole genome sequencing of several drug-resistant isolates of Candida glabrata collected from patients hospitalized in Eastern Poland to assess whether mutations in selected genes correlated with susceptibility analysis results. The fungal species from patient samples were identified, and the isolated Candida glabrata were subjected to antifungal drug susceptibility testing. The results were interpreted according to the EUCAST and CLSI recommendations. Susceptibility to 5-flucytosine was assessed using the ATB FUNGUS kit. Libraries were prepared according to the NEXTERA XT DNA Library Prep and subsequently sequenced. The outcomes indicated common resistance to two of the three analyzed echinocandins, as well as two cases of simultaneous resistance to echinocandins and selected azole-based drugs. We detected several previously reported mutations in selected resistance-related genes, as well as five that are first described here: ERG5 (M267I), ERG6 (R57K), PDH1 (K438Q, V434I, F600V, V1192S), FCY1 (M129T), and FCY2 (I384F). Neither of the identified nonsynonymous mutations was correlated with the drug resistance demonstrated in the susceptibility testing. Furthermore, we can exclude the possibility of acquired drug resistance, thereby raising questions about the possibility of unknown mechanisms of resistance to azole-based and echinocandin drugs.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-yield production and functional analysis of novel rhizobial-type glutaminase-free L-asparaginase from Paenibacillus thiaminolyticus.","authors":"Tomáš Podzimek, Karolína Loužecká, Veronika Urbánková, Jan Beránek, Petra Lipovová, Eva Benešová","doi":"10.1007/s12223-025-01296-y","DOIUrl":"https://doi.org/10.1007/s12223-025-01296-y","url":null,"abstract":"<p><p>L-Asparaginases are enzymes known for decades due to their use in medicine for the treatment of acute lymphoblastic leukemia. Recently, they have also found application in the food industry, and other possibilities are emerging in the treatment of infectious diseases or in the design of biosensors. For this reason, an ongoing effort has been made to find and characterize new enzymes with properties suitable for these specific applications. In this work, L-asparaginase from Paenibacillus thiaminolyticus (isoenzyme 1) belonging to the least explored group of L-asparaginases derived from L-asparaginase from Rhizobium etli was recombinantly produced with high yields (335 mg per L of culture medium) in E. coli cells and characterized: K_M = (26 ± 8) mmol L^-1, pH_opt = 10.3, without L-glutaminase and urease activity. A probable oligomeric structure (homodimer) was derived by computer modeling and confirmed by gel chromatography experiments. The results of this work extend the current limited knowledge about the poorly described class of R. etli L-asparaginases. Moreover, this L-asparaginase exhibits suitable properties for use in biosensor construction because of the high yields during recombinant production, K_M value, stability, and absence of L-glutaminase activity.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel and simple workflow for investigating Mycoplasma spp. contamination in cell cultures.","authors":"Nathalia de Freitas Michelon, José Valter Joaquim Silva Júnior, Rudi Weiblen, Eduardo Furtado Flores","doi":"10.1007/s12223-025-01297-x","DOIUrl":"https://doi.org/10.1007/s12223-025-01297-x","url":null,"abstract":"<p><p>Mycoplasma spp. contamination is a major concern in laboratories handling cell cultures, and routine detection methods are usually time-consuming, laborious and lack sensitivity. This study presents a streamlined workflow integrating rapid thermal DNA extraction (99 °C^-1 min) with a SYBR Green-based qPCR for Mycoplasma detection. High-coverage primers targeting an 86-bp region of the 16S rDNA were designed using 109 Mycoplasma spp. sequences from GeneBank. In silico analysis confirmed full primer annealing to major cell culture contaminants (M. arginini, M. hominis, M. orale, and M. hyorhinis). Upon thermal lysis and qPCR optimization, the yield of the protocol was equivalent to that of phenol-chloroform extraction plus qPCR, with a detection limit of 64 bacterial cells. Finally, the performance of the protocol was confirmed in cell cultures with known Mycoplasma spp. contamination, accurately reproducing the contamination status. Thus, the developed protocol provides a simple, rapid, cost-effective, and sensitive method for monitoring Mycoplasma spp. in cell cultures.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling a sustainable approach to cancer treatment: the antitumor activity of amygdalin and cell-free supernatant of Lacticaseibacillus rhamnosus.","authors":"Omnia Karem M Riad, Hagar F Forsan, Basma Abdulsamad, Heba Mohammed Refat M Selim, Wafaa S Khalaf","doi":"10.1007/s12223-025-01289-x","DOIUrl":"https://doi.org/10.1007/s12223-025-01289-x","url":null,"abstract":"<p><p>The pharmaceutical industry increasingly prioritizes environmental sustainability across its strategies, focusing on developing eco-friendly anti-cancer treatments. This is particularly important considering many anti-cancer drugs are not fully metabolized and pollute aquatic ecosystems. Amygdalin, a plant-derived compound, and cell-free supernatants (CFS) of Lacticaseibacillus rhamnosus show promise as sustainable anti-cancer agents. This study evaluated the potential anti-cancer activity of amygdalin and L. rhamnosus CFS against MCF-7 and A549 cancer cell lines. Cell viability was assessed using the MTT assay in response to monotherapy and combination therapy treatments. The induction of apoptosis was investigated using flow cytometric analyses. The combination demonstrated a synergistic effect on MCF-7 and A549 cell lines, with a combination index (CI) value of 0.8159 ± 0.0245 and 0.6422 ± 0.0316, respectively. Further analysis using propidium iodide (PI) staining and Annexin V confirmed increased apoptosis in combination-treated cells compared to those treated with amygdalin alone. These findings suggest that the combination of amygdalin and CFS of L. rhamnosus exhibits a synergistic anti-cancer effect against MCF-7 and A549 cells. Therefore, this combination is considered sustainable, eco-friendly, and effective anti-cancer treatment. This is the first study to investigate the anti-cancer effect of amygdalin in combination with L. rhamnosus cell-free supernatant (CFS) while considering its potential for environmental sustainability. Further research in a xenograft animal model is warranted to validate these findings.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Silviavirus phages with broad-host-range activity against methicillin resistant Staphylococcus aureus from seafood: comprehending the phenotypic and genotypic variability.","authors":"Karthika Raveendran, Sifana Sharaf, Ammu Lakshmi D, Reshmi K, Toms Cheriyath Joseph, Raja Swaminathan Thangaraj, Visnuvinayagam Sivam, Shashikanta Parida, Nagendra R Hegde, Ravindranath Shashidhar, Madhusudana Rao Badireddy, Vandan Nagar, Murugadas Vaiyapuri","doi":"10.1007/s12223-025-01290-4","DOIUrl":"https://doi.org/10.1007/s12223-025-01290-4","url":null,"abstract":"<p><p>Methicillin-resistant Staphylococcus aureus (MRSA) poses significant challenges to global health, attributed to their ability to resist multiple antibiotic classes. In the current situation, phage-based biocontrol strategies offer a promising alternative, leveraging their high specificity and efficacy against multidrug-resistant bacteria. The present study reports the phenotypic and genotypic characterizations of three broad-host-range MRSA phages: φCIFT_MFB_MRSA12, φCIFT_MFB_MRSA28, and φCIFT_MFB_MRSA32 for their application in seafood safety. The phages exhibited burst sizes ranging from 75 to 107 PFU/cell and burst periods of 80-90 min. The thermal and pH stability studies indicated that φCIFT_MFB_MRSA12 exhibited the highest thermal stability (- 20 to 60 °C), while φCIFT_MFB_MRSA28 demonstrated the widest pH tolerance (pH 3-12). The genomic analysis indicated that the phages possessed linear double-stranded DNA ranging from 141,193 to 141,505 bp, with large direct terminal repeats (DTRs) of 10,893 bp and various coding and non-coding genes (group-I introns, HEARO, and RAGATH). The comparative genome analysis revealed that three phages were found to be closely related to Silviavirus phages of the Herelleviridae family and differed with respect to tail fiber proteins, Ig domain-like carbohydrate-binding domains, and certain hypothetical proteins. Interestingly, the intergenomic and phylogenetic analyses revealed that the phages belonged to a novel species. Importantly, the genomes lacked virulence factors, antimicrobial resistance genes, or lysogenic determinants, supporting their safety in biocontrol strategies. The three Silviavirus phages could be potential candidates for the biocontrol of MRSA in seafood supply chains, thereby contributing to food safety and security.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of SAP1-3 genes in non-albicans Candida isolates in bovine raw milk and human sample.","authors":"Zahra Namvar, Abbas Akhavan Sepahy, Robab Rafiei Tabatabaei, Somayeh Sharifynia, Farzad Aala, Sassan Rezaie","doi":"10.1007/s12223-025-01284-2","DOIUrl":"https://doi.org/10.1007/s12223-025-01284-2","url":null,"abstract":"<p><p>Candida species are now taken as one of the essential opportunistic pathogens in clinical specimens. Although Candida albicans is one of the most essential opportunistic pathogens, non-albicans Candida (NAC) should not be taken for granted because these microorganisms are among the most common pathogens in patients today, leading to diseases, such as candidiasis, gastrointestinal tract infection, and vulvovaginitis. Fungal agents produce secreted aspartic proteinases (SAPs) to penetrate tissues. SAPs facilitate the invasion and colonization of host tissue by rupturing host mucosal membranes. They play an important role in weakening the structural and immunological defense proteins. The aim of this study was to compare the expression of SAP 1-3 genes in NAC isolated from different samples. We isolated the NAC such as Candida parapsilosis (C. parapsilosis), Candida tropicalis (C. tropicalis), Pichia kudriavzevii (P. kudriavzevii), and Nakaseomyces glabrata (N. glabrata) from two different sources of bovine raw milk and human samples. Then, we compared the expression of SAP1, SAP2, and SAP3 genes in both samples by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The results of gene expression showed that expression of the genes SAP2 and SAP3 was different in C. parapsilosis detected from raw milk and human samples. The expression of SAP2 was significantly decreased in human samples (**p < 0.01), whereas the expression of SAP3 was significantly increased in human samples (*p < 0.05). In some cases, the expression of these genes was similar among N. glabrata, P. kudriavzevii, and C. tropicalis. The expression of SAP2 and SAP3 genes in the same species of NAC from various sources is different.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative genome analysis of bacteriocin-producing Lactiplantibacillus pentosus LNP1-39 and its synbiotic role in suppressing food-borne pathogens.","authors":"Thanadol Jirakanjanasit, Natladda Choovet, Auttaporn Booncharoen, Engkarat Kingkaew, Saranporn Poothong, Somboon Tanasupawat, Sukanya Phuengjayaem","doi":"10.1007/s12223-025-01285-1","DOIUrl":"https://doi.org/10.1007/s12223-025-01285-1","url":null,"abstract":"<p><p>Lactic acid bacteria were isolated from traditional Thai-fermented foods. Among these, the strain LNP1-39, closely related to Lactiplantibacillus pentosus, was selected for further study because of its non-pathogenic profile. The bacteriocins produced by L. pentosus LNP1-39 were proteinaceous substances that exhibited strong antimicrobial activity across a wide pH range (pH 2-11; 6400-2400 AU/mL) and thermal stability at 100 °C for 40 min (400 AU/mL). These bacteriocins showed a narrow antimicrobial spectrum, effectively targeting Gram-positive pathogens, such as Kocuria rhizophila MIII, Enterococcus faecalis JCM 5803^T, and Listeria monocytogenes ATCC 19115. Comprehensive safety assessments, including whole-genome analysis and in vitro tests, confirmed a low risk of antibiotic resistance and the absence of virulence factors. Strain LNP1-39 was confirmed to be closely related to L. pentosus DSM 20314^T via digital DNA‒DNA hybridization (dDDH; 75.4%), with average nucleotide identity (ANI) at 96.56% ANIb and 97.22% ANIm values. Additionally, LNP1-39 produces pediocin with notable similarity (76.29% identity to pediocin) and presents low risks for antibiotic-resistance genes or transfer genes while providing antioxidant properties. Strain LNP1-39 survived harsh gastrointestinal tract conditions and exhibited a favorable prebiotic index and positive prebiotic activity score when paired with polydextrose or isomalto-oligosaccharide. These findings support L. pentosus LNP1-39 as potential bacteriocin-producing lactic acid bacteria for further application in food preservation and pathogen control or as a synbiotic.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of SAP1 confers vanillin tolerance in Saccharomyces cerevisiae.","authors":"Bora Kim","doi":"10.1007/s12223-025-01294-0","DOIUrl":"https://doi.org/10.1007/s12223-025-01294-0","url":null,"abstract":"<p><p>The objectives of this study were to identify genes associated with vanillin tolerance and to elucidate the link between vanillin tolerance and oxidative stress and the hog1 pathway activation in the yeast Saccharomyces cerevisiae for the production of biofuels and renewable chemicals from lignocellulosic biomass. In this study, one strain tolerant to vanillin stress was isolated through a transposon-mediated mutant library, and the disrupted gene was identified as SAP1. In addition, this phenotype associated with vanillin tolerance was confirmed by deletion and overexpressing of this corresponding gene. In response to vanillin stress, mutant strain was found to reduce intracellular level of reactive oxygen species (ROS), induce activation of Hog1p, and increase expression of the stress-response transcription factors MSN2/4 and genes mediated by stress response elements such as CTT1, HSP12, and DDR2.</p>","PeriodicalId":12346,"journal":{"name":"Folia microbiologica","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}