白色念珠菌中组成型ALS3的表达对酵母锁定的tet-NRG1和brg1Δ菌株有不同的影响。

IF 3.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Folia microbiologica Pub Date : 2025-07-01 DOI:10.1007/s12223-025-01288-y

Nicholas C Schena, Kassandra M Baker, Anna A Stark, Derek P Thomas, Ian A Cleary

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引用次数: 0

摘要

真菌病原体白色念珠菌产生许多参与粘附的表面蛋白。其中许多黏附素对不同的形态具有特异性。不能形成菌丝的菌株通常在附着表面和形成生物膜的能力上受损。为了更好地了解其中一种粘附素Als3p在酵母锁定菌株中的功能，我们在BRG1和NRG1这两个调控回路中基因之一表达改变的菌株中表达ALS3。我们检测的突变体BRG1和NRG1株在表型上相似。我们观察到ALS3的表达提高了两种菌株对玻璃的粘附力，但只有一种菌株改变了生物膜的形成能力，这种能力降低了。

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Constitutive ALS3 expression in Candida albicans has differential effects on yeast-locked tet-NRG1 and brg1Δ strains.

The fungal pathogen Candida albicans produces numerous surface proteins involved in adhesion. Many of these adhesins are specific to different morphological forms. Strains that are unable to form hyphae are generally impaired in their ability to adhere to surfaces and to form biofilms. To better understand the function of one of these adhesins, Als3p, in the context of yeast-locked strains, we expressed ALS3 in strains with altered expression of one of two genes that are part of a regulatory circuit, BRG1 and NRG1. The mutant BRG1 and NRG1 strains we examined were phenotypically similar. We observed that ALS3 expression improved the adhesion of both strains to glass but that only one had altered biofilm forming ability, which was decreased.

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来源期刊

Folia microbiologica 工程技术-生物工程与应用微生物

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.