{"title":"A highly salt-tolerant monoclonal antibody-based enzyme-linked immunosorbent assay for the rapid detection of phenylethanolamine A in urine","authors":"Minggang Liu, Yuchen Bai, Leina Dou, Yihui Kong, Zhanhui Wang, K. Wen, Jianzhong Shen","doi":"10.1080/09540105.2022.2084043","DOIUrl":"https://doi.org/10.1080/09540105.2022.2084043","url":null,"abstract":"ABSTRACT Phenylethanolamine A (PEAA), as a typical β2-adrenoceptor agonist (β-AA), was widely illegally used in feed to improve the lean meat ratio. The detection performance of immunoassay, which is one of the effective methods to monitor PEAA residues in animal urine, has been hindered by high concentration of salt. Herein, we produced one highly salt-tolerant monoclonal antibody (mAb) 3E2 by hybridoma technology. Homologous modelling and molecular docking were used to analyse the antibody salt tolerance mechanism. Results show that the tight hydrophobic binding cavity is the key to high tolerance. Then the mAb 3E2 was used for specific detecting PEAA by enzyme-linked immunosorbent assay (ELISA) with the IC50 value of 0.36 ng mL−1and the LOD value of 0.065 µg L−1. Benefiting from the high salt tolerance of antibodies, swine urine and sheep urine spiked with PEAA can be detected directly by ELISA, and the acceptable recovery rates of 80.1–108.8% were obtained.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45750293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methods of simultaneous preparation of various active substances from Stichopus chloronotus and functional evaluation of active substances","authors":"Yundan Xia, Changwei Wang, Dejun Yu, Hu Hou","doi":"10.1080/09540105.2022.2100322","DOIUrl":"https://doi.org/10.1080/09540105.2022.2100322","url":null,"abstract":"ABSTRACT\u0000 Stichopus chloronotus, a marine sea cucumber rich in n-3 PUFAs and phenolic compounds, is a deep black–green sea cucumber with yellow/ red papillae tips, which has important economic and ecological value. In this project, a process was established to simultaneous extract and prepare four different active substances (including sea cucumber polysaccharide, polypeptide, saponin and lipid) from Stichopus chloronotus. The yields and purity were also improved. Except for polysaccharides, the purity of the other three active substances was above 90%. The extraction rates were all above 60%. In addition, the functional activity of sea cucumber peptides was evaluated. The results showed that sea cucumber peptides could reduce triglyceride content in mice serum, increase glutathione content and relieve alcohol-induced liver damage. The technical research laid a technical foundation for the high value utilization of Stichopus chloronotus.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44409401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Soriano-Romaní, J. A. Nieto, Sandra García-Benlloch
{"title":"Immunomodulatory role of edible bone collagen peptides on macrophage and lymphocyte cell cultures","authors":"L. Soriano-Romaní, J. A. Nieto, Sandra García-Benlloch","doi":"10.1080/09540105.2022.2098936","DOIUrl":"https://doi.org/10.1080/09540105.2022.2098936","url":null,"abstract":"ABSTRACT Immunonutrition or modulation of immune capacity through food and supplements has been gaining significant importance. Hydrolysed collagen has long been used as a functional ingredient, showing multiple physiological activities, including enhancement of immune functions. However, how collagen peptides may affect the immune system still needs further research. This study investigates bone collagen peptides (BCP) immunomodulatory activity on human monocytic THP-1 and human Jurkat T lymphocyte cell lines, using cytokine mRNA expressions as biomarkers. In vitro, gastrointestinal digestion and Caco-2 cell absorption allow obtaining digested and absorbed BCP fractions, respectively, which are tested on immune cells. Results show: (1) Immunostimulatory effect on M0 macrophages, but not on M1 macrophages (lipopolysaccharide (LPS)-activated), (2) Significant T lymphocyte proliferation after incubation with absorbed BCP fraction. (3) Significant increase of anti-inflammatory IL-10 cytokine biomarker. These results suggest that BCP could act as an immunonutrient, modulating the immune response and inflammatory processes. Abbreviations: ATCC: American Type Culture Collection BCP: bone collagen peptide DMSO: dimethyl sulphoxide FBS: fetal bovine serum ECM: extracellular matrix GRAS: Generally recognized as safe; IFN: interferon IL: interleukin, LPS: lipopolysaccharide SEM: standard error of the mean TEER: transepithelial electrical resistance","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42457940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant and anti-inflammatory activities of Gynura procumbens flowers extract through suppressing LPS-induced MAPK/NF-κB signalling pathways","authors":"Ming-Yuan Cao, Jing Wu, Chuan-Qi Xie, Lei Wu, Zhen Gu, Ju-Wu Hu, Wei Xiong","doi":"10.1080/09540105.2022.2098935","DOIUrl":"https://doi.org/10.1080/09540105.2022.2098935","url":null,"abstract":"<p><b>ABSTRACT</b></p><p><i>Gynura procumbens</i> is a traditional herb and food extensively cultivated in China and Southeast Asian countries. This study was designed to determine the content of the main chemical components and elucidate the antioxidant and anti-inflammatory capacity of <i>G. procumbens</i> flowers extracts (GPFE). GPFE was rich in flavonoids and phenolics and exhibited great reducing power as well as strong scavenging ability of ABTS<sup>+</sup> and DPPH radicals. The pro-inflammatory factors including IL-6, IL-1β, NO, TNF-α, PGE<sub>2</sub> and their mRNA transcriptions were strongly inhibited with the treatment of GPFE in LPS-stimulated RAW264.7 macrophages. Meanwhile, GPFE could attenuate the inflammation via suppressing the nuclear translocation of NF-κB p65 and downregulating the MAPK signalling pathways. Furthermore, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C isolated from GPFE all had a high content and showed potent anti-inflammatory activities. Our findings suggest that GPFE could be used as a natural anti-inflammatory agent.</p>","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138504068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Chaingam, Rattanathorn Choonong, T. Juengwatanatrakul, T. Kanchanapoom, W. Putalun, G. Yusakul
{"title":"Evaluation of anti-inflammatory properties of Eurycoma longifolia Jack and Eurycoma harmandiana Pierre in vitro cultures and their constituents","authors":"J. Chaingam, Rattanathorn Choonong, T. Juengwatanatrakul, T. Kanchanapoom, W. Putalun, G. Yusakul","doi":"10.1080/09540105.2022.2100324","DOIUrl":"https://doi.org/10.1080/09540105.2022.2100324","url":null,"abstract":"ABSTRACT Eurycoma longifolia Jack (EL) has been used for treating erectile dysfunction, in which inflammation plays a major role. EL tissue cultures can overcome the shortage of material; however, the anti-inflammatory property of such cultures has not been investigated. Herein, we evaluated the anti-inflammatory effects of root and callus cultures of EL and of a related species, Eurycoma harmandiana Pierre (EH), on lipopolysaccharide-activated RAW 264.7 cells. Eurycomalactone and 9-methoxycanthin-6-one were the predominant compounds in EL calli. Eurycomanone content was 0.283 mg/g in EL root culture and 0.348 mg/g in intact roots. The extracts of the root and callus cultures significantly inhibited nitric oxide production, and inhibited the expression of iNOS, IL-6, and COX-2 genes. Overall, the tissue cultures of EH and EL produced eurycomanone, chaparinone, eurycomalactone, 9-hydroxycanthin-6-one, canthin-6-one, and 9-methoxycanthin-6-one, which exhibited anti-inflammatory effects. These tissue cultures may be a valuable and sustainable source of active ingredients effective against inflammatory conditions.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45310638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Čelakovská, E. Čermáková, R. Vankova, P. Boudková, C. Andrys, J. Krejsek
{"title":"Kiwi allergy in atopic dermatitis patients – analysis of specific IgE results in ALEX2 multiplex examination. Latex fruit syndrome","authors":"J. Čelakovská, E. Čermáková, R. Vankova, P. Boudková, C. Andrys, J. Krejsek","doi":"10.1080/09540105.2022.2095985","DOIUrl":"https://doi.org/10.1080/09540105.2022.2095985","url":null,"abstract":"ABSTRACT Our study analyses the sensitisation profile to kiwi allergens in patients suffering from atopic dermatitis with the use of ALEX2 Allergy Explorer test. The sensitisation to molecular components of latex, banana, avocado, pollen, seeds within latex fruit syndrome was also evaluated. Altogether 100 atopic dermatitis patients were examined. The incidence of clinical reaction to kiwi was observed in 15% of patients; a combination of the kiwi allergens Act d 1, Act d 2 and Act d 10 gave a diagnostic sensitivity of 33.3%. The latex fruit syndrome was recorded in one patient (1%) with the positive result to Hev b 6.02, to (Pers a) and oral allergy syndrome after kiwi ingestion. The sensitisation both to molecular components of kiwi and latex was recorded in 7% of patients. Further research on kiwi allergy should focus on the importance of chitinase and 2S albumins.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44944871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. B. Bayazid, Young-Ah Jang, Soo Ah Jeong, B. Lim
{"title":"Cypress tree (Chamaecyparis obtusa) Bark extract inhibits melanogenesis through repressing CREB and MITF signalling pathways in α-MSH-stimulated B16F10 cells","authors":"A. B. Bayazid, Young-Ah Jang, Soo Ah Jeong, B. Lim","doi":"10.1080/09540105.2022.2095986","DOIUrl":"https://doi.org/10.1080/09540105.2022.2095986","url":null,"abstract":"ABSTRACT Cypress tree (Chamaecyparis obtusa) bark is well-known for its bio-functional activities and high content of polyphenol and flavonoids. It has previously exhibited antioxidant, anti-pathogenic, and anti-inflammatory activities. Our study aimed to investigate the anti-melanogenic effect of Cypress Tree Bark extract (CBE). We evaluated cellular tyrosinase activity and melanin content in alpha-melanocyte-stimulating hormone (α-MSH) induced B16F10 murine melanoma cells. We analyzed microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP1 and TRP2), and cAMP response element-binding protein (CREB) activation via phosphorylation of AKT and ERK using western blot analysis. Tyrosinase, MITF, TRP1, and TRP2 mRNA expression were examined via real-time polymerase chain reaction. CBE restored melanin content and tyrosinase activity remarkably in α-MSH stimulated melanoma cells. It exhibited an anti-melanogenic effect through suppressing MITF, TRP1, TRP2, tyrosinase mRNA and protein expression in α-MSH-induced B16F10 cells. Furthermore, CBE has significantly inhibited CREB activation by suppressing AKT and extracellular signal-regulated kinase phosphorylation. Our data strongly suggest that CBE has potential effects against melanogenesis. GRAPHICAL ABSTRACT Graphical Abstract: The proposed mechanism of CBE on anti-melanogenic effect.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41274823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Čelakovská, E. Čermáková, R. Vankova, P. Boudková, J. Krejsek, C. Andrys
{"title":"Sensitivity, specificity and positive predictive value of ALEX2 multiplex examination in patients suffering from atopic dermatitis and reaction to egg","authors":"J. Čelakovská, E. Čermáková, R. Vankova, P. Boudková, J. Krejsek, C. Andrys","doi":"10.1080/09540105.2022.2085672","DOIUrl":"https://doi.org/10.1080/09540105.2022.2085672","url":null,"abstract":"ABSTRACT\u0000 The aim is to evaluate the sensitivity, specificity and positive predictive value of ALEX2 Allergy Explorer test in patients suffering from atopic dermatitis and reactions to egg. We compared the results of specific IgE to allergen reagents (molecular components) with reactions to egg in the open exposure test (OET) (history); the sensitivity, specificity and positive predictictive value were calculated with Fisher's Exact test. Altogether 100 atopic dermatitis patients were examined. The reaction to egg was confirmed in 19 patients (19%). We confirmed significant relation between the results of specific IgE to Gal d 1, Gal d 2, Gal d 3, Gal d 5, Gal d white and Gal d yolk and the results of the OET/history; the sensitivity is in the range 21.05–52.63%. We recommend to evaluate food allergy to egg in patients with severe atopic dermatitis even with negative specific IgE for molecular components.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46343678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cold plasma technology: fundamentals and effect on quality of meat and its products","authors":"Javeed Akhtar, Mebrhit Gebremariam Abrha, Kiros Teklehaimanot, Gebremeskel Gebrekirstos","doi":"10.1080/09540105.2022.2095987","DOIUrl":"https://doi.org/10.1080/09540105.2022.2095987","url":null,"abstract":"ABSTRACT Meat quality is damaged by traditional technologies. Researchers devised a revolutionary technique called plasma technology to meet the requirement for an effective cold processing method. Cold plasma (CP) used for meats and its products decontamination and storability extension has shown to be highly successful. The influence of CP on meat quality is important to its acceptability as an alternative meat processing technology. CP treatments have had effects on the color, pH, lipid oxidation, and microbial quality of different meat products. CP treatment offers crucial benefits over traditional processing methods because of its design adaptability, non-thermal behavior, relatively inexpensive, and environmental friendliness. CP processing is currently in its infancy and requires further investigation in meat and meat products to achieve its best extent. This paper discusses the fundamentals of CP technology, its effects on the quality of meats such as color, pH value, lipid oxidation, and microbial quality, and future perspectives.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42678609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Mu, Yinghong Xu, Gang Li, Shuo Dai, Qi Tong, Bin Liu
{"title":"Determination of glucosamine and galactosamine in food by liquid chromatography with pre-column derivatization","authors":"Li Mu, Yinghong Xu, Gang Li, Shuo Dai, Qi Tong, Bin Liu","doi":"10.1080/09540105.2022.2085673","DOIUrl":"https://doi.org/10.1080/09540105.2022.2085673","url":null,"abstract":"ABSTRACT A pre-column derivatization liquid chromatographic method was developed for the simultaneous determination of both glucosamine and galactosamine in selected food samples. The derivatization of glucosamine and galactosamine with 9-fluorenylmethoxycarbonyl chloride was accomplished under alkaline conditions and UV irradiation. When the detection wavelength was 265 nm and acetonitrile-0.1% phosphoric acid was the mobile phase at a flow rate of 1.0 mL/min, the linear range of ultraviolet intensity of glucosamine and galactosamine was 10–100 μg/mL,correlation coefficient of glucosamine was 0.9994 and correlation coefficient of galactosamine was 0.9993. The standard recovery of three kinds of food was in the range of 94.09–114.5%, the relative standard deviation (RSD) was 0.38–5.29%, the detection limit was 1.0 mg/kg, and the quantitative limit was 4.0 mg/kg. The results of this research indicate that this method is suitable for the simultaneous determination of glucosamine and galactosamine in food.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41974183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}