{"title":"Antioxidant and anti-inflammatory activities of Gynura procumbens flowers extract through suppressing LPS-induced MAPK/NF-κB signalling pathways","authors":"Ming-Yuan Cao, Jing Wu, Chuan-Qi Xie, Lei Wu, Zhen Gu, Ju-Wu Hu, Wei Xiong","doi":"10.1080/09540105.2022.2098935","DOIUrl":null,"url":null,"abstract":"<p><b>ABSTRACT</b></p><p><i>Gynura procumbens</i> is a traditional herb and food extensively cultivated in China and Southeast Asian countries. This study was designed to determine the content of the main chemical components and elucidate the antioxidant and anti-inflammatory capacity of <i>G. procumbens</i> flowers extracts (GPFE). GPFE was rich in flavonoids and phenolics and exhibited great reducing power as well as strong scavenging ability of ABTS<sup>+</sup> and DPPH radicals. The pro-inflammatory factors including IL-6, IL-1β, NO, TNF-α, PGE<sub>2</sub> and their mRNA transcriptions were strongly inhibited with the treatment of GPFE in LPS-stimulated RAW264.7 macrophages. Meanwhile, GPFE could attenuate the inflammation via suppressing the nuclear translocation of NF-κB p65 and downregulating the MAPK signalling pathways. Furthermore, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C isolated from GPFE all had a high content and showed potent anti-inflammatory activities. Our findings suggest that GPFE could be used as a natural anti-inflammatory agent.</p>","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2022-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Agricultural Immunology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/09540105.2022.2098935","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
ABSTRACT
Gynura procumbens is a traditional herb and food extensively cultivated in China and Southeast Asian countries. This study was designed to determine the content of the main chemical components and elucidate the antioxidant and anti-inflammatory capacity of G. procumbens flowers extracts (GPFE). GPFE was rich in flavonoids and phenolics and exhibited great reducing power as well as strong scavenging ability of ABTS+ and DPPH radicals. The pro-inflammatory factors including IL-6, IL-1β, NO, TNF-α, PGE2 and their mRNA transcriptions were strongly inhibited with the treatment of GPFE in LPS-stimulated RAW264.7 macrophages. Meanwhile, GPFE could attenuate the inflammation via suppressing the nuclear translocation of NF-κB p65 and downregulating the MAPK signalling pathways. Furthermore, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C isolated from GPFE all had a high content and showed potent anti-inflammatory activities. Our findings suggest that GPFE could be used as a natural anti-inflammatory agent.
期刊介绍:
Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed.
The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on:
-Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer
-Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals
-Effects of food on the immune system
-Studies on allergy and allergic reactions
-Investigations into food allergies
-Development of allergen-free food systems
-Development of novel assay formats
-Applications of assay systems to the monitoring of food items in relation to safety and labelling
-Food quality issues, e.g. speciation, adulteration and contamination
-Comparisons between different analytical techniques
The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.