Antioxidant and anti-inflammatory activities of Gynura procumbens flowers extract through suppressing LPS-induced MAPK/NF-κB signalling pathways

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED

Food and Agricultural Immunology Pub Date : 2022-07-13 DOI:10.1080/09540105.2022.2098935

Ming-Yuan Cao, Jing Wu, Chuan-Qi Xie, Lei Wu, Zhen Gu, Ju-Wu Hu, Wei Xiong

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Abstract

ABSTRACT

Gynura procumbens is a traditional herb and food extensively cultivated in China and Southeast Asian countries. This study was designed to determine the content of the main chemical components and elucidate the antioxidant and anti-inflammatory capacity of G. procumbens flowers extracts (GPFE). GPFE was rich in flavonoids and phenolics and exhibited great reducing power as well as strong scavenging ability of ABTS⁺ and DPPH radicals. The pro-inflammatory factors including IL-6, IL-1β, NO, TNF-α, PGE₂ and their mRNA transcriptions were strongly inhibited with the treatment of GPFE in LPS-stimulated RAW264.7 macrophages. Meanwhile, GPFE could attenuate the inflammation via suppressing the nuclear translocation of NF-κB p65 and downregulating the MAPK signalling pathways. Furthermore, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C isolated from GPFE all had a high content and showed potent anti-inflammatory activities. Our findings suggest that GPFE could be used as a natural anti-inflammatory agent.

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通过抑制lps诱导的MAPK/NF-κB信号通路的抗氧化和抗炎活性

原藜是中国和东南亚国家广泛种植的传统草本植物和食品。本研究旨在测定原藜花提取物(GPFE)的主要化学成分含量，阐明其抗氧化和抗炎作用。GPFE含有丰富的黄酮类和酚类物质，对ABTS+和DPPH自由基具有很强的还原能力和清除能力。GPFE对lps刺激的RAW264.7巨噬细胞的促炎因子IL-6、IL-1β、NO、TNF-α、PGE2及其mRNA转录均有明显抑制作用。同时，GPFE可以通过抑制NF-κB p65核易位和下调MAPK信号通路来减轻炎症。此外，从GPFE中分离得到的异绿原酸B、异绿原酸A和异绿原酸C含量较高，具有较强的抗炎活性。我们的研究结果表明，GPFE可以作为一种天然的抗炎剂。

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.

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