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Food and Agricultural Immunology最新文献

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Metabolomics and water migration analysis provides valuable insights into nutrient generation in Tartary buckwheat (Fagopyrum tataricum) seed germination 代谢组学和水分迁移分析为苦麦种子发芽过程中的营养生成提供了有价值的见解
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-09-19 DOI: 10.1080/09540105.2022.2117797
Bai Yongliang, Xin Meiguo, Lin Roumin, He Weijun, He Shuyan, Z. Rong, Guo Yiping
{"title":"Metabolomics and water migration analysis provides valuable insights into nutrient generation in Tartary buckwheat (Fagopyrum tataricum) seed germination","authors":"Bai Yongliang, Xin Meiguo, Lin Roumin, He Weijun, He Shuyan, Z. Rong, Guo Yiping","doi":"10.1080/09540105.2022.2117797","DOIUrl":"https://doi.org/10.1080/09540105.2022.2117797","url":null,"abstract":"ABSTRACT Seed germination of Tartary buckwheat is important for its biological generation of various nutrients. To investigate the nutrient differences during seed germination, the metabolic profiling and moisture status of Tartary buckwheat seed germination were quantitatively measured by ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS) and NMR, respectively. The results showed water was predominantly in free status during the three stages of seed germination divided. Statistical analysis (PCA, OPLS-DA, KEGG) of 53 metabolites at different germination stages revealed the key metabolites of linoleic, flavonoid and phenylalanine biosynthesis during the seed germination. Furthermore, flavonoids biosynthesis was identified as the pathway with the largest difference significance at the second and third stages, while linoleic and phenylalanine metabolisms were screened out as the major pathways with most impact factors in the first and second and the third stage, respectively. The analysis provided valuable insights into the nutrient generation during Tartary buckwheat seeds germination.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"692 - 708"},"PeriodicalIF":3.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44024267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish 快速酶联免疫吸附法和胶体金免疫测定法测定贝类中冈田酸及其衍生物
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-31 DOI: 10.1080/09540105.2022.2115466
Ruirui Wang, Hong-bo Qi, Miao Liang, Guoxiang Liao, Fan Yang
{"title":"Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish","authors":"Ruirui Wang, Hong-bo Qi, Miao Liang, Guoxiang Liao, Fan Yang","doi":"10.1080/09540105.2022.2115466","DOIUrl":"https://doi.org/10.1080/09540105.2022.2115466","url":null,"abstract":"ABSTRACT Okadaic acid (OA) and the analogs of dinophysistoxin (DTX) are important members of diarrhetic shellfish poisoning (DSP) toxins. In this study, five OA-specific mAbs (monoclonal antibodies) were developed from five stable cells of hybridoma. The anti-OA mAb-2D7 showed high sensitivity to OA, the IC50 of the antibody was 0.24 ng/mL, and its cross-reactivity was 91.6% with DTX-1 and 110.5% with DTX-2., In dcELISA, the IC50 was set at 0.182 ng/mL, and the detection limit was set at 0.023 ng/mL by using the anti-OA mAb-2D7. The level of OA recovered from spiked mussel samples was 2–50 ng/g, ranging from 97.6 ± 7.2% to 106.4 ± 9.8%. In contrast, the immunostrip assay with a limit of 5 ng/mL, conducted for detecting OA, was completed in 10 min. The mAb-based dcELISA and immunostrip assay developed were precise and sensitive enough to quickly assess OA, DTX-1, and DTX-2 in shellfish specimens.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"677 - 691"},"PeriodicalIF":3.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46487863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Curcumin inhibits invasion and metastasis of human hepatoma cells through Bclaf1-mediated Wnt/β-catenin signalling 姜黄素通过bclaf1介导的Wnt/β-catenin信号传导抑制人肝癌细胞的侵袭和转移
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-28 DOI: 10.1080/09540105.2022.2113864
Zhongwei Zhao, Jielin Su, Jiaqi Zhao, Jiaxin Chen, Xinmu Cui, Manqing Sun, Xuewu Zhang
{"title":"Curcumin inhibits invasion and metastasis of human hepatoma cells through Bclaf1-mediated Wnt/β-catenin signalling","authors":"Zhongwei Zhao, Jielin Su, Jiaqi Zhao, Jiaxin Chen, Xinmu Cui, Manqing Sun, Xuewu Zhang","doi":"10.1080/09540105.2022.2113864","DOIUrl":"https://doi.org/10.1080/09540105.2022.2113864","url":null,"abstract":"ABSTRACT Curcumin, a kind of natural compound extracted from the rhizome of Zingiberaceae such as turmeric, has many pharmacological effects such as anti-cancer effects. This study investigated the effect of curcumin on the invasion and metastasis of hepatocellular carcinoma (HCC) cell lines HepG2 and SK-Hep-1 through the Wnt/β-catenin signalling pathway and the regulatory mechanism of Bcl-2-associated transcription factor 1 (Bclaf1). Curcumin significantly inhibited the migration and invasion of HepG2 and SK-Hep-1 cells and inhibited the Wnt/β-catenin signalling pathway and reduced Bclaf1 expression in human hepatoma cells. In nude mice, intraperitoneal injection of curcumin significantly inhibited the growth of subcutaneously transplanted tumours and reduced lung metastasis of the tumour cells, downregulated the expression of Bclaf1, and inhibited the Wnt/β-catenin pathway. This study suggests that curcumin is a novel candidate drug to prevent cancer metastasis and that Bclaf1 is a new gene target related to the proliferation, invasion, and metastasis of hepatocellular carcinoma.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"664 - 676"},"PeriodicalIF":3.0,"publicationDate":"2022-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45563165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Identification and characterization of MicroRNAs in pig liver after the LPS challenge using RNA-seq 利用RNA-seq技术鉴定和表征LPS攻毒后猪肝中的microrna
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-22 DOI: 10.1080/09540105.2022.2109602
Yang Yang, Qingqing Lv, Xingfa Huang, Jiajun Fan, Pei Li, Huijuan Zhu, P. Kang, Yulan Liu
{"title":"Identification and characterization of MicroRNAs in pig liver after the LPS challenge using RNA-seq","authors":"Yang Yang, Qingqing Lv, Xingfa Huang, Jiajun Fan, Pei Li, Huijuan Zhu, P. Kang, Yulan Liu","doi":"10.1080/09540105.2022.2109602","DOIUrl":"https://doi.org/10.1080/09540105.2022.2109602","url":null,"abstract":"ABSTRACT This study was conducted to test the expression profiles of miRNAs in the liver in piglets after the LPS challenge using sequencing technology. Twelve barrows were assigned to two groups, including the saline group and the LPS group. Six small RNA (sRNA) libraries were constructed, and novel miRNAs were identified using mirevo and mirdeep2 software and validated by quantitative real-time PCR (qRT-PCR). The results showed the mRNA expression of IL-1β, IL-6 and TNF-α in the liver increased after the LPS challenge (P < .05). A total of 29 differentially expressed miRNAs were identified in the liver after the LPS challenge. And 11 miRNAs were validated by qRT-PCR. In addition, the results of statistics of pathway enrichment showed that these miRNAs could be related to lipid metabolism response. This study provides the first miRNA expression profiles of LPS-mediated changes in the liver, which might provide potential insights into miRNAs involved in regulating lipid metabolism in pigs challenged by LPS.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"652 - 663"},"PeriodicalIF":3.0,"publicationDate":"2022-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48546669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Solanum nigrum induces macrophage activation through TLR4-mediated activation of JNK and macrophage autophagy through TLR4-mediated activation of p38 and JNK 龙葵通过TLR4介导的JNK活化诱导巨噬细胞活化，并通过TLR4中介导的p38和JNK活化介导巨噬细胞自噬
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-16 DOI: 10.1080/09540105.2022.2113507
Julia Yu, Min Yeong Choi, So-Jung Park, Na Gyeong Geum, Jae Won Lee, G. Park, H. Eo, J. Jeong
{"title":"Solanum nigrum induces macrophage activation through TLR4-mediated activation of JNK and macrophage autophagy through TLR4-mediated activation of p38 and JNK","authors":"Julia Yu, Min Yeong Choi, So-Jung Park, Na Gyeong Geum, Jae Won Lee, G. Park, H. Eo, J. Jeong","doi":"10.1080/09540105.2022.2113507","DOIUrl":"https://doi.org/10.1080/09540105.2022.2113507","url":null,"abstract":"ABSTRACT In this study, we investigated the effect of Solanum nigrum aerial parts (SNAP) on macrophage activation and macrophage autophagy in RAW264.7 cells. SNAP increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. TLR4 inhibition blocked SNAP-mediated production of immunostimulatory factors. In addition, the JNK inhibition reduced the SNAP-mediated production of immunostimulatory factors, and the SNAP-mediated JNK activation was blocked by the TLR4 inhibition. SNAP activated macrophage autophagy. TLR4 inhibition blocked SNAP-mediated macrophage autophagy and inhibition of p38 and JNK attenuated SNAP-mediated macrophage autophagy. These findings indicate that SNAP may induce TLR4/JNK-mediated macrophage activation and TLR4/p38 and JNK-mediated macrophage autophagy.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"641 - 651"},"PeriodicalIF":3.0,"publicationDate":"2022-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44820149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immune-enhancing effects of Hibiscus syriacus roots in RAW264.7 macrcophages 木槿根对RAW264.7巨噬细胞的免疫增强作用
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-16 DOI: 10.1080/09540105.2022.2107621
H. Eo, Yunmi Park, H. Kwon, G. Park
{"title":"Immune-enhancing effects of Hibiscus syriacus roots in RAW264.7 macrcophages","authors":"H. Eo, Yunmi Park, H. Kwon, G. Park","doi":"10.1080/09540105.2022.2107621","DOIUrl":"https://doi.org/10.1080/09540105.2022.2107621","url":null,"abstract":"ABSTRACT In this study, we evaluated whether extracts of the roots of Hibiscus syriacus (HSR) exert immune activation activities and elucidated its potential mechanism in macrophages. The HSR dose-dependently increased the production of immunomodulators such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and tumor necrosis factor (TNF-α) activated phagocytosis in macrophages. Inhibition of toll-like receptors 4 (TLR4) reduced the production of immunomodulators induced by HSR. Inhibition of mitogen-activated protein kinase (MAPKs), nuclear factor-κB (NF-κB) and phosphoinositide-3 kinase (PI3K) signaling attenuated the production of immunomodulators induced by HSR. Based on the results of this study, HSR was thought to activate macrophages through the production of immunomodulators and phagocytosis activation through TLR4-dependent MAPKs, NF-κB and PI3K signaling pathways. Therefore, it is thought that the HSR has the potential to be used as agents for enhancing immunity.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"617 - 626"},"PeriodicalIF":3.0,"publicationDate":"2022-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48567130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design and synthesis of geosmin derivatives using organic synthesis strategies and application in antibody production 利用有机合成策略设计、合成geosmin衍生物及其在抗体生产中的应用
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-11 DOI: 10.1080/09540105.2022.2107620
Penghui Zhou, Ying Liu, Ting Zhou, Hanwen Liu, Jianguo Li, Anping Deng
{"title":"Design and synthesis of geosmin derivatives using organic synthesis strategies and application in antibody production","authors":"Penghui Zhou, Ying Liu, Ting Zhou, Hanwen Liu, Jianguo Li, Anping Deng","doi":"10.1080/09540105.2022.2107620","DOIUrl":"https://doi.org/10.1080/09540105.2022.2107620","url":null,"abstract":"ABSTRACT To develop a sensitive and specific ELISA for geosmin (GSM), the research of this study is focused on the design and synthesis of several GSM derivatives, which maintain the whole GSM structure as much as possible and contain a spacer arm with an active group at the end. To form the GSM backbone, tandem organic reaction strategies were used to replace the traditional Robinson cyclization reaction. Five GSM derivatives were synthesized and the formed GSM derivative-protein conjugates were used as the immunogens for the production of polyclonal antibodies against GSM, while four GSM derivatives were synthesized and the GSM derivative-protein conjugates were used as coating antigens for establishing ELISA. The relationship between the structures of GSM derivatives and the antibody properties was explored. Under optimal conditions, the LOD of the ELISA for GSM was found to be 0.16 ng mL−1, and the antiserum was able to specifically recognize the GSM backbone.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"627 - 640"},"PeriodicalIF":3.0,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46988164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taraxasterol repairs UVB-induced skin barrier injury through MAPK/NF-κB signaling pathways 蒲公英醇通过MAPK/NF-κB信号通路修复uvb诱导的皮肤屏障损伤
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-04 DOI: 10.1080/09540105.2022.2107619
Yuzhi Zhang, Hao Fu, Yongtao Zhang, Dongdong Wang, Dan Zhao, Jiachan Zhang, Meng Li, Changtao Wang
{"title":"Taraxasterol repairs UVB-induced skin barrier injury through MAPK/NF-κB signaling pathways","authors":"Yuzhi Zhang, Hao Fu, Yongtao Zhang, Dongdong Wang, Dan Zhao, Jiachan Zhang, Meng Li, Changtao Wang","doi":"10.1080/09540105.2022.2107619","DOIUrl":"https://doi.org/10.1080/09540105.2022.2107619","url":null,"abstract":"ABSTRACT\u0000 Taraxasterol (TAL) is a pentacyclic triterpenoid compound, which has anti-inflammatory effect. Cytotoxicity assay was used to determine the optimal concentration of TAL and positive control dipotassium glycyrrhizinate (DG), and the optimal dose of UVB. Experimental data indicate that TAL has scavenging activity against UVB radiation-induced intracellular reactive oxygen species (ROS) compared to UVB controls. The contents of skin barrier-related factors in the groups were detected by Enzyme-linked immunosorbent assay (ELISA), then ELISA and quantitative polymerase chain reaction (qPCR) were used to detect changes in the inflammatory factors, apoptosis factors, and gene levels in the groups. Therefore, TAL stabilised the levels of inflammation, apoptosis, and skin barrier-related factors by regulating Mitogen-activated protein kinases/nuclear factor-k-gene binding (MAPK/NF-κB) signalling pathways, such as jun-amino-terminal kinase (JNK), p38, extracellular signal-regulated kinase (ERK), and NF-κB. These results suggest that TAL repairs UVB-induced skin barrier damage by scavenging reactive oxygen species and regulating the MAPK/NF-κB signalling pathway.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"604 - 616"},"PeriodicalIF":3.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44824368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Sensitisation to molecular components of mites in atopic dermatitis patients 特应性皮炎患者对螨虫分子成分的致敏
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-08-04 DOI: 10.1080/09540105.2022.2100323
J. Čelakovská, E. Čermáková, R. Vankova, P. Boudková, C. Andrys, J. Krejsek
{"title":"Sensitisation to molecular components of mites in atopic dermatitis patients","authors":"J. Čelakovská, E. Čermáková, R. Vankova, P. Boudková, C. Andrys, J. Krejsek","doi":"10.1080/09540105.2022.2100323","DOIUrl":"https://doi.org/10.1080/09540105.2022.2100323","url":null,"abstract":"ABSTRACT Background and aim: To analyse the sensitisation profile to molecular components of mites allergens with the use of ALEX2 Allergy Explorer test in Atopic dermatitis (AD) patients. Method: The complete dermatological and allergological examination including the examination of the sensitisation to molecular components with ALEX2 Allergy Explorer testing was performed. For the statistical analysis, we used Fisher's Exact. Results and conclusion: The sensitisation to mites increases with AD severity. The central role in atopic march may play the molecular component Der f 2 and Der p 23. The results of our study confirm the important role of Der p 5 and Der p 7 in subgroup of patients suffering from bronchial asthma. The specific immunotherapy should focus on the components of NPC2 family and Der p 23 in AD patients. Our results may be helpful in planning hyposensitisation, treatment and immunotherapy.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"588 - 603"},"PeriodicalIF":3.0,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48422219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A highly salt-tolerant monoclonal antibody-based enzyme-linked immunosorbent assay for the rapid detection of phenylethanolamine A in urine 基于高耐盐单克隆抗体的酶联免疫吸附法快速检测尿液中苯乙醇胺A
IF 3 3区 农林科学
Food and Agricultural Immunology Pub Date : 2022-07-29 DOI: 10.1080/09540105.2022.2084043
Minggang Liu, Yuchen Bai, Leina Dou, Yihui Kong, Zhanhui Wang, K. Wen, Jianzhong Shen
{"title":"A highly salt-tolerant monoclonal antibody-based enzyme-linked immunosorbent assay for the rapid detection of phenylethanolamine A in urine","authors":"Minggang Liu, Yuchen Bai, Leina Dou, Yihui Kong, Zhanhui Wang, K. Wen, Jianzhong Shen","doi":"10.1080/09540105.2022.2084043","DOIUrl":"https://doi.org/10.1080/09540105.2022.2084043","url":null,"abstract":"ABSTRACT Phenylethanolamine A (PEAA), as a typical β2-adrenoceptor agonist (β-AA), was widely illegally used in feed to improve the lean meat ratio. The detection performance of immunoassay, which is one of the effective methods to monitor PEAA residues in animal urine, has been hindered by high concentration of salt. Herein, we produced one highly salt-tolerant monoclonal antibody (mAb) 3E2 by hybridoma technology. Homologous modelling and molecular docking were used to analyse the antibody salt tolerance mechanism. Results show that the tight hydrophobic binding cavity is the key to high tolerance. Then the mAb 3E2 was used for specific detecting PEAA by enzyme-linked immunosorbent assay (ELISA) with the IC50 value of 0.36 ng mL−1and the LOD value of 0.065 µg L−1. Benefiting from the high salt tolerance of antibodies, swine urine and sheep urine spiked with PEAA can be detected directly by ELISA, and the acceptable recovery rates of 80.1–108.8% were obtained.","PeriodicalId":12300,"journal":{"name":"Food and Agricultural Immunology","volume":"33 1","pages":"575 - 587"},"PeriodicalIF":3.0,"publicationDate":"2022-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45750293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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