利用RNA-seq技术鉴定和表征LPS攻毒后猪肝中的microrna

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED

Food and Agricultural Immunology Pub Date : 2022-08-22 DOI:10.1080/09540105.2022.2109602

Yang Yang, Qingqing Lv, Xingfa Huang, Jiajun Fan, Pei Li, Huijuan Zhu, P. Kang, Yulan Liu

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引用次数: 1

摘要

本研究采用测序技术检测LPS攻毒后仔猪肝脏中mirna的表达谱。将12只雄鼠分为生理盐水组和LPS组。构建6个小RNA (sRNA)文库，利用mirevo和mirdeep2软件鉴定新的mirna，并采用实时荧光定量PCR (qRT-PCR)进行验证。结果显示，LPS刺激后肝脏中IL-1β、IL-6和TNF-α mRNA表达升高(P < 0.05)。LPS刺激后，肝脏中共鉴定出29种差异表达的mirna。通过qRT-PCR对11个mirna进行了验证。此外，途径富集的统计结果表明，这些mirna可能与脂质代谢反应有关。本研究首次提供了LPS介导的肝脏变化的miRNA表达谱，这可能为LPS刺激下参与调节猪脂质代谢的miRNA提供潜在的见解。

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Identification and characterization of MicroRNAs in pig liver after the LPS challenge using RNA-seq

ABSTRACT This study was conducted to test the expression profiles of miRNAs in the liver in piglets after the LPS challenge using sequencing technology. Twelve barrows were assigned to two groups, including the saline group and the LPS group. Six small RNA (sRNA) libraries were constructed, and novel miRNAs were identified using mirevo and mirdeep2 software and validated by quantitative real-time PCR (qRT-PCR). The results showed the mRNA expression of IL-1β, IL-6 and TNF-α in the liver increased after the LPS challenge (P < .05). A total of 29 differentially expressed miRNAs were identified in the liver after the LPS challenge. And 11 miRNAs were validated by qRT-PCR. In addition, the results of statistics of pathway enrichment showed that these miRNAs could be related to lipid metabolism response. This study provides the first miRNA expression profiles of LPS-mediated changes in the liver, which might provide potential insights into miRNAs involved in regulating lipid metabolism in pigs challenged by LPS.

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.