Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for assessing okadaic acid and its derivatives in shellfish

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED

Food and Agricultural Immunology Pub Date : 2022-08-31 DOI:10.1080/09540105.2022.2115466

Ruirui Wang, Hong-bo Qi, Miao Liang, Guoxiang Liao, Fan Yang

引用次数: 4

Abstract

ABSTRACT Okadaic acid (OA) and the analogs of dinophysistoxin (DTX) are important members of diarrhetic shellfish poisoning (DSP) toxins. In this study, five OA-specific mAbs (monoclonal antibodies) were developed from five stable cells of hybridoma. The anti-OA mAb-2D7 showed high sensitivity to OA, the IC50 of the antibody was 0.24 ng/mL, and its cross-reactivity was 91.6% with DTX-1 and 110.5% with DTX-2., In dcELISA, the IC50 was set at 0.182 ng/mL, and the detection limit was set at 0.023 ng/mL by using the anti-OA mAb-2D7. The level of OA recovered from spiked mussel samples was 2–50 ng/g, ranging from 97.6 ± 7.2% to 106.4 ± 9.8%. In contrast, the immunostrip assay with a limit of 5 ng/mL, conducted for detecting OA, was completed in 10 min. The mAb-based dcELISA and immunostrip assay developed were precise and sensitive enough to quickly assess OA, DTX-1, and DTX-2 in shellfish specimens.

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快速酶联免疫吸附法和胶体金免疫测定法测定贝类中冈田酸及其衍生物

冈田酸（OA）及其类似物是腹泻性贝类中毒（DSP）毒素的重要组成部分。在本研究中，从五种稳定的杂交瘤细胞中开发了五种OA特异性单克隆抗体。抗OA mAb-2D7对OA具有较高的敏感性，其IC50为0.24ng/mL，与DTX-1和DTX-2的交叉反应性分别为91.6%和110.5%。从加标贻贝样品中回收的OA水平为2–50纳克/克，范围为97.6 ± 7.2%至106.4 ± 9.8%。相比之下，免疫跳闸试验的限值为5 用于检测OA的ng/mL在10分钟内完成。开发的基于mAb的dcELISA和免疫跳闸测定足够精确和灵敏，可以快速评估贝类标本中的OA、DTX-1和DTX-2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.