FEMS yeast research最新文献_第4页

Riboflavin overproduction on lignocellulose hydrolysate by the engineered yeast Candida famata. 工程酵母法氏念珠菌在木质纤维素水解物上过量产生核黄素。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae020

Ljubov S Dzanaeva, Dominik Wojdyła, Dariya V Fedorovych, Justyna Ruchala, Kostyantyn V Dmytruk, Andriy A Sibirny

{"title":"Riboflavin overproduction on lignocellulose hydrolysate by the engineered yeast Candida famata.","authors":"Ljubov S Dzanaeva, Dominik Wojdyła, Dariya V Fedorovych, Justyna Ruchala, Kostyantyn V Dmytruk, Andriy A Sibirny","doi":"10.1093/femsyr/foae020","DOIUrl":"10.1093/femsyr/foae020","url":null,"abstract":"Lignocellulose (dry plant biomass) is an abundant cheap inedible residue of agriculture and wood industry with great potential as a feedstock for biotechnological processes. Lignocellulosic substrates can serve as valuable resources in fermentation processes, allowing the production of a wide array of chemicals, fuels, and food additives. The main obstacle for cost-effective conversion of lignocellulosic hydrolysates to target products is poor metabolism of the major pentoses, xylose and L-arabinose, which are the second and third most abundant sugars of lignocellulose after glucose. We study the oversynthesis of riboflavin in the flavinogenic yeast Candida famata and found that all major lignocellulosic sugars, including xylose and L-arabinose, support robust growth and riboflavin synthesis in the available strains of C. famata. To further increase riboflavin production from xylose and lignocellulose hydrolysate, genes XYL1 and XYL2 coding for xylose reductase and xylitol dehydrogenase were overexpressed. The resulting strains exhibited increased riboflavin production in both shake flasks and bioreactors using diluted hydrolysate, reaching 1.5 g L-1.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Yca1 metacaspase: diverse functions determine how yeast live and let die. 更正：Yca1元天冬酶：多种功能决定酵母如何生存和死亡

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae019

引用次数: 0

Heterologous pulcherrimin production in Saccharomyces cerevisiae confers inhibitory activity on Botrytis conidiation. 在酿酒酵母中异源生产 pulcherrimin 可对肉毒菌分生孢子产生抑制作用。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foad053

Florian M Freimoser, Marina Mahler, Mark McCullough, Alexander O Brachmann, Lukas Nägeli, Maja Hilber-Bodmer, Jörn Piel, Stefan A Hoffmann, Yizhi Cai

{"title":"Heterologous pulcherrimin production in Saccharomyces cerevisiae confers inhibitory activity on Botrytis conidiation.","authors":"Florian M Freimoser, Marina Mahler, Mark McCullough, Alexander O Brachmann, Lukas Nägeli, Maja Hilber-Bodmer, Jörn Piel, Stefan A Hoffmann, Yizhi Cai","doi":"10.1093/femsyr/foad053","DOIUrl":"10.1093/femsyr/foad053","url":null,"abstract":"Pulcherrimin is an iron (III) chelate of pulcherriminic acid that plays a role in antagonistic microbial interactions, iron metabolism, and stress responses. Some bacteria and yeasts produce pulcherriminic acid, but so far, pulcherrimin could not be produced in Saccharomyces cerevisiae. Here, multiple integrations of the Metschnikowia pulcherrima PUL1 and PUL2 genes in the S. cerevisiae genome resulted in red colonies, which indicated pulcherrimin formation. The coloration correlated positively and significantly with the number of PUL1 and PUL2 genes. The presence of pulcherriminic acid was confirmed by mass spectrometry. In vitro competition assays with the plant pathogenic fungus Botrytis caroliana revealed inhibitory activity on conidiation by an engineered, strong pulcherrimin-producing S. cerevisiae strain. We demonstrate that the PUL1 and PUL2 genes from M. pulcherrima, in multiple copies, are sufficient to transfer pulcherrimin production to S. cerevisiae and represent the starting point for engineering and optimizing this biosynthetic pathway in the future.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10786192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138884786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data integration strategies for whole-cell modeling. 全细胞建模的数据整合策略。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae011

Katja Tummler, Edda Klipp

引用次数: 0

Exploring yeast biodiversity and process conditions for optimizing ethylene glycol conversion into glycolic acid. 探索酵母生物多样性和工艺条件，优化乙二醇转化为乙醇酸的过程。

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae024

Vittorio Giorgio Senatore, Riccardo Milanesi, Fiorella Masotti, Letizia Maestroni, Stefania Pagliari, Ciro Cannavacciuolo, Luca Campone, Immacolata Serra, Paola Branduardi

{"title":"Exploring yeast biodiversity and process conditions for optimizing ethylene glycol conversion into glycolic acid.","authors":"Vittorio Giorgio Senatore, Riccardo Milanesi, Fiorella Masotti, Letizia Maestroni, Stefania Pagliari, Ciro Cannavacciuolo, Luca Campone, Immacolata Serra, Paola Branduardi","doi":"10.1093/femsyr/foae024","DOIUrl":"10.1093/femsyr/foae024","url":null,"abstract":"Plastics have become an indispensable material in many fields of human activities, with production increasing every year; however, most of the plastic waste is still incinerated or landfilled, and only 10% of the new plastic is recycled even once. Among all plastics, polyethylene terephthalate (PET) is the most produced polyester worldwide; ethylene glycol (EG) is one of the two monomers released by the biorecycling of PET. While most research focuses on bacterial EG metabolism, this work reports the ability of Saccharomyces cerevisiae and nine other common laboratory yeast species not only to consume EG, but also to produce glycolic acid (GA) as the main by-product. A two-step bioconversion of EG to GA by S. cerevisiae was optimized by a design of experiment approach, obtaining 4.51 ± 0.12 g l-1 of GA with a conversion of 94.25 ± 1.74% from 6.21 ± 0.04 g l-1 EG. To improve the titer, screening of yeast biodiversity identified Scheffersomyces stipitis as the best GA producer, obtaining 23.79 ± 1.19 g l-1 of GA (yield 76.68%) in bioreactor fermentation, with a single-step bioprocess. Our findings contribute in laying the ground for EG upcycling strategies with yeasts.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11344169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sphingolipid diversity in Candida auris: unraveling interclade and drug resistance fingerprints. 白色念珠菌的鞘脂多样性：揭示支链间和耐药性指纹。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foae008

Basharat Ali, Mohit Kumar, Praveen Kumar, Anshu Chauhan, Sana Akhtar Usmani, Shivaprakash M Rudramurthy, Jacques F Meis, Arunaloke Chakrabarti, Ashutosh Singh, Naseem A Gaur, Alok K Mondal, Rajendra Prasad

{"title":"Sphingolipid diversity in Candida auris: unraveling interclade and drug resistance fingerprints.","authors":"Basharat Ali, Mohit Kumar, Praveen Kumar, Anshu Chauhan, Sana Akhtar Usmani, Shivaprakash M Rudramurthy, Jacques F Meis, Arunaloke Chakrabarti, Ashutosh Singh, Naseem A Gaur, Alok K Mondal, Rajendra Prasad","doi":"10.1093/femsyr/foae008","DOIUrl":"10.1093/femsyr/foae008","url":null,"abstract":"In this study, we explored the sphingolipid (SL) landscape in Candida auris, which plays pivotal roles in fungal biology and drug susceptibility. The composition of SLs exhibited substantial variations at both the SL class and molecular species levels among clade isolates. Utilizing principal component analysis, we successfully differentiated the five clades based on their SL class composition. While phytoceramide (PCer) was uniformly the most abundant SL class in all the isolates, other classes showed significant variations. These variations were not limited to SL class level only as the proportion of different molecular species containing variable number of carbons in fatty acid chains also differed between the isolates. Also a comparative analysis revealed abundance of ceramides and glucosylceramides in fluconazole susceptible isolates. Furthermore, by comparing drug-resistant and susceptible isolates within clade IV, we uncovered significant intraclade differences in key SL classes such as high PCer and low long chain base (LCB) content in resistant strains, underscoring the impact of SL heterogeneity on drug resistance development in C. auris. These findings shed light on the multifaceted interplay between genomic diversity, SLs, and drug resistance in this emerging fungal pathogen.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10941814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of unimodal probability distribution models for morphological phenotyping of budding yeast. 单模态概率分布模型在芽殖酵母形态表型中的应用

IF 2.4 4区生物学

FEMS yeast research Pub Date : 2024-01-09 DOI: 10.1093/femsyr/foad056

Yoshikazu Ohya, Farzan Ghanegolmohammadi, Kaori Itto-Nakama

{"title":"Application of unimodal probability distribution models for morphological phenotyping of budding yeast.","authors":"Yoshikazu Ohya, Farzan Ghanegolmohammadi, Kaori Itto-Nakama","doi":"10.1093/femsyr/foad056","DOIUrl":"10.1093/femsyr/foad056","url":null,"abstract":"Morphological phenotyping of the budding yeast Saccharomyces cerevisiae has helped to greatly clarify the functions of genes and increase our understanding of cellular functional networks. It is necessary to understand cell morphology and perform quantitative morphological analysis (QMA) but assigning precise values to morphological phenotypes has been challenging. We recently developed the Unimodal Morphological Data image analysis pipeline for this purpose. All true values can be estimated theoretically by applying an appropriate probability distribution if the distribution of experimental values follows a unimodal pattern. This reliable pipeline allows several downstream analyses, including detection of subtle morphological differences, selection of mutant strains with similar morphology, clustering based on morphology, and study of morphological diversity. In addition to basic research, morphological analyses of yeast cells can also be used in applied research to monitor breeding and fermentation processes and control the fermentation activity of yeast cells.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139086547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antioxidant activity of Fe(III), Mn(III)Mn(II), and Cu(II) compounds in Saccharomyces cerevisiae and Galleria mellonella models of study 探究铁（III）、锰（III）、锰（II）和铜（II）化合物在酿酒酵母和黑僵菌模型中的抗氧化活性

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-12-21 DOI: 10.1093/femsyr/foad052

Larissa M M Mattos, Hyan M Hottum, Daniele C Pires, Bruna B Segat, Adolfo Horn, Christiane Fernandes, Marcos D Pereira

{"title":"Exploring the antioxidant activity of Fe(III), Mn(III)Mn(II), and Cu(II) compounds in Saccharomyces cerevisiae and Galleria mellonella models of study","authors":"Larissa M M Mattos, Hyan M Hottum, Daniele C Pires, Bruna B Segat, Adolfo Horn, Christiane Fernandes, Marcos D Pereira","doi":"10.1093/femsyr/foad052","DOIUrl":"https://doi.org/10.1093/femsyr/foad052","url":null,"abstract":"Reactive oxygen species (ROS) are closely related to oxidative stress, aging, and the onset of human diseases. To mitigate ROS-induced damages, extensive research has focused on examining the antioxidative attributes of various synthetic/natural substances. Coordination compounds serving as synthetic antioxidants have emerged as a promising approach to attenuate ROS toxicity. Herein, we investigated the antioxidant potential of a series of Fe(III) (1), Mn(III)Mn(II) (2) and Cu(II) (3) coordination compounds synthesized with the ligand N-(2-hydroxybenzyl)-N-(2-pyridylmethyl)[(3-chloro)(2-hydroxy)]-propylamine in Saccharomyces cerevisiae exposed to oxidative stress. We also assessed the antioxidant potential of these complexes in the alternative model of study, Galleria mellonella. DPPH analysis indicated that these complexes presented moderate antioxidant activity. However, treating Saccharomyces cerevisiae with 1, 2 and 3 increased the tolerance against oxidative stress and extended yeast lifespan. The treatment of yeast cells with these complexes decreased lipid peroxidation and catalase activity in stressed cells, whilst no change in SOD activity was observed. Moreover, these complexes induced the Hsp104 expression. In G. mellonella, complex administration extended larval survival under H2O2 stress and did not affect the insect's life cycle. Our results suggest that the antioxidant potential exhibited by these complexes could be further explored to mitigate various oxidative stress-related disorders.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138824596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QTL mapping reveals novel genes and mechanisms underlying variations in H2S production during alcoholic fermentation in Saccharomyces cerevisiae QTL 图谱揭示了酿酒酵母在酒精发酵过程中产生 H2S 的新基因和新机制

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-12-16 DOI: 10.1093/femsyr/foad050

Irene De Guidi, Céline Serre, Jessica Noble, Anne Ortiz-Julien, Bruno Blondin, Jean-Luc Legras

{"title":"QTL mapping reveals novel genes and mechanisms underlying variations in H2S production during alcoholic fermentation in Saccharomyces cerevisiae","authors":"Irene De Guidi, Céline Serre, Jessica Noble, Anne Ortiz-Julien, Bruno Blondin, Jean-Luc Legras","doi":"10.1093/femsyr/foad050","DOIUrl":"https://doi.org/10.1093/femsyr/foad050","url":null,"abstract":"Saccharomyces cerevisiae requirement for reduced sulfur to synthesise methionine and cysteine during alcoholic fermentation, is mainly fulfilled through the sulfur assimilation pathway. S. cerevisiae reduces sulfate into sulfur dioxide (SO2) and sulfide (H2S), whose overproduction is a major issue in winemaking, due to its negative impact on wine aroma. The amount of H2S produced is highly strain-specific and also depends on SO2 concentration, often added to grape must. Applying a Bulk Segregant Analysis to a 96 strain-progeny derived from two strains with different abilities to produce H2S, and comparing allelic frequencies along the genome of pools of segregants producing contrasting H2S quantities, we identified two causative regions involved in H2S production in the presence of SO2. A functional genetic analysis allowed the identification of variants in four genes able to impact H2S formation, viz; ZWF1, ZRT2, SNR2 and YLR125W, and involved in functions and pathways not associated with sulfur metabolism until now. These data point out that, in wine fermentation conditions, redox status and zinc homeostasis are linked to H2S formation while providing new insights into the regulation of H2S production, and a new vision of the interplay between the sulfur assimilation pathway and cell metabolism.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138824212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

My Journey with Yeast. 我的酵母之旅。

IF 3.2 4区生物学

FEMS yeast research Pub Date : 2023-06-22 DOI: 10.1093/femsyr/foad035

Charles Abbas

引用次数: 0