FEMS yeast research最新文献_第5页

Overexpression of the MAPK transcription factor gene MSS11 enhances ethanol biosynthesis in Saccharomyces cerevisiae via coordinated metabolic and stress-response reprogramming. MAPK转录因子基因MSS11的过表达通过协调代谢和应激反应重编程促进酿酒酵母的乙醇生物合成

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag024

Xu Qiu, Yangyang Liu, Aili Zhang

{"title":"Overexpression of the MAPK transcription factor gene MSS11 enhances ethanol biosynthesis in Saccharomyces cerevisiae via coordinated metabolic and stress-response reprogramming.","authors":"Xu Qiu, Yangyang Liu, Aili Zhang","doi":"10.1093/femsyr/foag024","DOIUrl":"10.1093/femsyr/foag024","url":null,"abstract":"Bioethanol is a pivotal sustainable alternative to fossil fuels. Saccharomyces cerevisiae is the primary microorganism for its industrial production. Enhancing the fermentative performance of S. cerevisiae is crucial for sustainable bioethanol production. To systematically enhance the fermentative capacity of yeast, this study employed targeted genetic engineering to overexpress MSS11-a principal transcription factor within the mitogen-activated protein kinase (MAPK) signaling cascade. The resultant recombinant strain, Eng-1, demonstrated a marked 23.9% enhancement in final ethanol titer (18.94 g/l vs. 15.29 g/l) and a commensurate 23.8% improvement in yield coefficient (0.473 vs. 0.382 g ethanol per g glucose) relative to the isogenic control strain Eng-0 under microaerobic batch fermentation. This phenotype was consistently validated in a distinct genetic background (JT139 derivative), underscoring the generalizable function of MSS11. Transcriptomic analysis revealed that MSS11 orchestrates a dual regulatory mechanism: it upregulates pyruvate-supplying pathway genes to enhance carbon flux toward ethanol, while concurrently activating a suite of genes involved in oxidative, osmotic, and ethanol stress tolerance to improve cellular robustness. These findings establish MSS11 as a novel and effective metabolic engineering target for yeast, and they elucidate its dual regulatory mechanism in coordinately enhancing carbon conversion and cellular robustness, thereby providing a strategic framework for constructing high-performance microbial cell factories for advanced biofuel production.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13289602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148143265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive laboratory evolution of maltose utilization in wild Irish isolates of Saccharomyces eubayanus. 爱尔兰真芽酵母菌野生分离株麦芽糖利用的适应性实验室进化。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag036

Sean Bergin, Jillian Scully, Conor Hession, Juliane da Silveira, Matthieu Osborne, Calum Nolan, Róisín O'Neill, Hans-Georg Eckhardt, Lisa Lombardi, Kenneth H Wolfe, Geraldine Butler

{"title":"Adaptive laboratory evolution of maltose utilization in wild Irish isolates of Saccharomyces eubayanus.","authors":"Sean Bergin, Jillian Scully, Conor Hession, Juliane da Silveira, Matthieu Osborne, Calum Nolan, Róisín O'Neill, Hans-Georg Eckhardt, Lisa Lombardi, Kenneth H Wolfe, Geraldine Butler","doi":"10.1093/femsyr/foag036","DOIUrl":"10.1093/femsyr/foag036","url":null,"abstract":"The first European isolates of Saccharomyces eubayanus were discovered in Ireland in 2022 and belong to the same clade as the S. eubayanus parent of the hybrid lager yeast Saccharomyces pastorianus. Here, we report the isolation of 10 additional Irish strains and we explore maltose metabolism. Maltose metabolism genes are clustered in subtelomeric MAL loci including maltase (MALS), maltose transporter (MALT), and regulator (MALR). Despite having intact MAL loci, the Irish strains do not grow on maltose. Two Irish S. eubayanus strains (UCD650 and UCD926) were passaged in medium containing maltose as the sole carbon source until they acquired the ability to metabolize maltose. The UCD650-derived lineage acquired an I243N substitution in MalR, and a 30-kb duplication of the MAL locus. The UCD926 parent has a similar MAL locus duplication and its evolved lineage acquired a W307L substitution in MalR. Expression of MALT and MALS was increased in the evolved isolates. Introducing the W307L mutation into UCD926 by CRISPR-Cas9 editing fully recapitulates the maltose utilization phenotype. The I243N variant alone has little effect on expression of maltose genes. We find that maltose utilization is influenced both by the copy number of MAL genes and by gain-of-function mutations in MalR.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13479922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering yeast for sustainable bioproduction of tryptophan-derived aromatic compounds. 可持续生物生产色氨酸衍生芳香化合物的工程酵母。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag012

Baode Sun, Xinqi Xie, Axayacatl González, Tim McCubbin, Esteban Marcellin, Huadong Peng

引用次数: 0

Yeast strain development and process intensification in high-gravity fermentation. 高重力发酵中酵母菌株的开发与工艺强化。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag046

Son Chu-Ky, Tuan-Anh Pham, Ngoc-Hung Pham, Tien-Thanh Nguyen, Chinh-Nghia Nguyen, Tien-Cuong Nguyen, Tuan Le, Thuy-Hang Dam, Lan-Huong Nguyen, Thi Minh Tu Nguyen, Thi Anh-Tuyet Nguyen, Lan-Huong Phung, Quyet-Tien Phi, Quynh-Anh Pham, Quoc-Phong Truong, Kien-Cuong Pham, Luong Hung Tien, Minh Nhat Dang, Tien-Nam Tien, Joe Eun Son, Sung Keun Jung, Ui Wook Hwang, Soo Rin Kim

{"title":"Yeast strain development and process intensification in high-gravity fermentation.","authors":"Son Chu-Ky, Tuan-Anh Pham, Ngoc-Hung Pham, Tien-Thanh Nguyen, Chinh-Nghia Nguyen, Tien-Cuong Nguyen, Tuan Le, Thuy-Hang Dam, Lan-Huong Nguyen, Thi Minh Tu Nguyen, Thi Anh-Tuyet Nguyen, Lan-Huong Phung, Quyet-Tien Phi, Quynh-Anh Pham, Quoc-Phong Truong, Kien-Cuong Pham, Luong Hung Tien, Minh Nhat Dang, Tien-Nam Tien, Joe Eun Son, Sung Keun Jung, Ui Wook Hwang, Soo Rin Kim","doi":"10.1093/femsyr/foag046","DOIUrl":"10.1093/femsyr/foag046","url":null,"abstract":"High- and very-high-gravity (HG/VHG) fermentation increases substrate loading and product titers, thereby improving fermenter utilization and potentially reducing water use and downstream processing requirements. Initially developed for brewing and fuel ethanol production, these approaches are now applied more broadly in food, beverage, and bioproduct manufacturing. This MiniReview summarises operational definitions and industrial drivers of HG/VHG fermentation and examines the associated constraints in rheology, mass and heat transfer, osmotic and ethanol stress, nutrient availability, and oxidative damage. Yeast improvement strategies are reviewed, including adaptive laboratory evolution, mutagenesis, genome shuffling, multiplex genome editing, non-conventional yeasts, and multi-omics-guided selection. Process developments such as no-cook simultaneous liquefaction, saccharification and fermentation, enzyme formulation, nutrient management, and in situ product recovery are considered together with applications in alcoholic beverages, organic acids, microbial lipids, and other value-added products. The review also discusses coproduct valorization and the need to integrate strain development with process design. Current evidence supports HG/VHG fermentation as a useful process-intensification platform, although performance and sustainability depend strongly on feedstock, operating conditions, product requirements, and the basis used to report fermentation outcomes.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13599774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148944620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A refined Saccharomyces cerevisiae reference transcriptome from direct RNA sequencing, with a reusable pipeline for UTR annotation updates. 来自Direct RNA测序的精制酿酒酵母参考转录组，具有可重复使用的UTR注释更新管道。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag052

Orlane Rossini, Alice Cleynen, Nikolay E Shirokikh

{"title":"A refined Saccharomyces cerevisiae reference transcriptome from direct RNA sequencing, with a reusable pipeline for UTR annotation updates.","authors":"Orlane Rossini, Alice Cleynen, Nikolay E Shirokikh","doi":"10.1093/femsyr/foag052","DOIUrl":"10.1093/femsyr/foag052","url":null,"abstract":"Untranslated regions (UTRs) flanking the coding sequence govern mRNA translation, localization, and decay, making accurate UTR boundaries essential for quantitative RNA sequencing and the study of post-transcriptional control. Reference transcriptomes built from short-read sequencing have been invaluable to the yeast community, yet in a genome as gene-dense as that of Saccharomyces cerevisiae, short reads cannot be assigned to a single transcript of origin, leaving roughly one quarter of transcripts without a defined UTR. Here, we use direct RNA sequencing (DRS), in which native polyadenylated molecules are sequenced without fragmentation or amplification, to reduce this ambiguity and deliver two complementary resources. First, an updated S. cerevisiae S288C reference: change-point segmentation of per-gene DRS coverage defined boundaries for 5682 of the 6607 genes in the backbone model, and a merge-max rule retaining the longer UTR from each source ensures no gene loses existing annotation. The result adds previously absent UTRs to 1213 (5') and 1220 (3') of the genes that the earlier short-read annotation left unannotated, and extends 28.9% of 5' and 19.4% of 3' boundaries among comparable genes. Second, the complete, documented pipeline so that the transcriptome can be rebuilt from any DRS data. Validation on two independent datasets shows improved mapping rates, reduced soft-clipping, and metagene profiles consistent with genuine transcript signal.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13637595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the potential of oxidative and weakly fermentative yeasts in biocontrol and bioprotection. 利用氧化酵母和弱发酵酵母在生物防治和生物保护中的潜力。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag053

Evelyn Maluleke, Heinrich Wilbur du Plessis, Neil Paul Jolly, Mathabatha Evodia Setati

{"title":"Harnessing the potential of oxidative and weakly fermentative yeasts in biocontrol and bioprotection.","authors":"Evelyn Maluleke, Heinrich Wilbur du Plessis, Neil Paul Jolly, Mathabatha Evodia Setati","doi":"10.1093/femsyr/foag053","DOIUrl":"10.1093/femsyr/foag053","url":null,"abstract":"There is increasing demand for sustainable alternatives to chemical fungicides and preservatives due to the negative impact that they have on the environment, human and animal health. This has led to intensified interest in microbial biocontrol and bioprotection strategies. Oxidative and weakly fermentative yeasts represent an underexplored group of microorganisms with considerable potential for managing undesirable microorganisms. These yeasts produce limited amounts of ethanol and carbon dioxide while exhibiting diverse mechanisms of antagonism against spoilage and pathogenic microorganisms. Their biocontrol and bioprotection activity is mediated through nutrient and space competition, antimicrobial metabolites and hydrolytic enzymes production, biofilm formation, induction of host resistance, and modulation of microbial communities. These characteristics make such yeasts particularly attractive for applications in fresh produce, fermented beverages, and agricultural systems where minimal alteration of the final product is desirable. Furthermore, their reduced fermentative activity lowers the risk of undesirable sensory changes while maintaining effective suppression of fruit and beverage spoilage microorganisms. Despite these advantages, their commercial application remains limited. This review examines current knowledge of oxidative and weakly fermentative yeasts as biological control agents, highlights their applications in fruits and beverages, factors affecting their efficacy, and challenges for their integration into sustainable biocontrol and bioprotection strategies.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13679385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The oxidative cost of losing low temperature viability protein 1 [Ltv1]: insights into cellular damage and homeostasis. 低温存活蛋白1 （Ltv1）的氧化损失：对细胞损伤和稳态的研究。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag019

McKayla Remines, Samuel A Ammerman, Jill B Keeney, Erin D Strome

引用次数: 0

Nutrient sensing and transceptor-mediated metabolic control in yeast. 酵母的营养感知和受体介导的代谢控制。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag027

Ryoya Tanahashi, Hiroshi Takagi, Akira Nishimura

{"title":"Nutrient sensing and transceptor-mediated metabolic control in yeast.","authors":"Ryoya Tanahashi, Hiroshi Takagi, Akira Nishimura","doi":"10.1093/femsyr/foag027","DOIUrl":"10.1093/femsyr/foag027","url":null,"abstract":"The yeast Saccharomyces cerevisiae coordinates growth, metabolism, and stress adaptation through signaling pathways that respond to changes in nutrient availability. Classical nutrient-sensing systems, including the cAMP-protein kinase A (PKA), Snf1/AMP-activated protein kinase, target of rapamycin complex 1 (TORC1)-Sch9, Ssy1-Ptr3-Ssy5, and general amino acid control pathways, have revealed how yeast senses and responds to extracellular carbon, nitrogen, phosphate, and amino acid levels. In addition to these established pathways, plasma-membrane nutrient transporters also function in signaling rather than solely mediating substrate uptake. These dual-function proteins, termed nutrient transceptors, couple nutrient transport or extracellular nutrient recognition to rapid intracellular responses, often activating PKA without detectable changes in cAMP levels. This review focuses on yeast nutrient transceptors, specifically Gap1, Mep2, Pho84, Sul1/Sul2, Can1, Ftr1, and Zrt1. These proteins link extracellular nutrient availability to intracellular regulatory responses, including trehalose mobilization, stress resistance, growth resumption, filamentous development, and, in some cases, TORC1-Sch9 signaling. Mechanistic insights, including transport-signaling uncoupling and potential physical association with downstream protein kinases, are also discussed. Collectively, this evidence establishes nutrient transceptors as an essential additional layer of nutrient sensing in yeast, highlighting their role in translating extracellular nutrient cues into cellular responses.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13377633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148351438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cysteine, methionine, and pantothenic acid remodel the Saccharomyces cerevisiae transcriptome and volatile sulfur compound metabolome during alcoholic fermentation. 半胱氨酸、蛋氨酸和泛酸在酒精发酵过程中重塑酿酒酵母的转录组和挥发性硫化合物代谢组。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag022

Rafael Jiménez-Lorenzo, James D Duncan, Valérie Nolleau, Vincent Farines, Jean-Marie Sablayrolles, Audrey Bloem, Carole Camarasa

{"title":"Cysteine, methionine, and pantothenic acid remodel the Saccharomyces cerevisiae transcriptome and volatile sulfur compound metabolome during alcoholic fermentation.","authors":"Rafael Jiménez-Lorenzo, James D Duncan, Valérie Nolleau, Vincent Farines, Jean-Marie Sablayrolles, Audrey Bloem, Carole Camarasa","doi":"10.1093/femsyr/foag022","DOIUrl":"10.1093/femsyr/foag022","url":null,"abstract":"Yeast nitrogen and vitamin nutrition are fundamental levers for managing wine quality, yet the specific mechanisms linking nutrient availability to sulfur aroma formation remain poorly understood. This study explored the impact of methionine, cysteine, and pantothenic acid (vitamin B5)-nutrients with direct, pivotal roles in sulfur metabolic pathways-on the Saccharomyces cerevisiae transcriptome and VSC metabolome during fermentation. Our findings reveal that methionine and cysteine catabolic routes act as isolated compartments; the genes responsible for bridging these two pathways remain transcriptionally silent in the presence of high cysteine or methionine availability. This lack of metabolic crossover leads to highly specific VSC signatures. Methionine exerts only a limited influence on global gene expression and primarily drives the production of methylthio-compounds via the Ehrlich pathway. Cysteine triggers a starvation-like transcriptomic response and promotes a diverse range of thiols and thioesters. Pantothenic acid deficiency compromised yeast growth and fermentation efficiency, triggered extensive transcriptional changes in sulfur assimilation pathways, effectively redirecting flux toward non-Ehrlich catabolic products. Overall, this study provides a robust mechanistic basis for how sulfur amino acid and pantothenic acid levels can be targeted to modulate wine aroma profiles and prevent the development of reductive off-flavors.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13348253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearing Candida biofilms using combined antimicrobial sophorolipids with amphotericin B and itraconazole. 抗菌皂荚脂与两性霉素B和伊曲康唑联合清除念珠菌生物膜。

IF 3.5 4区生物学

FEMS yeast research Pub Date : 2026-01-05 DOI: 10.1093/femsyr/foag021

L A Channa B Jayasekara, Kwanrutai Watchaputi, Jirayut Euanorasetr, Kanokwan Poomputsa, Chutikarn Butkinaree, Nitnipa Soontorngun

{"title":"Clearing Candida biofilms using combined antimicrobial sophorolipids with amphotericin B and itraconazole.","authors":"L A Channa B Jayasekara, Kwanrutai Watchaputi, Jirayut Euanorasetr, Kanokwan Poomputsa, Chutikarn Butkinaree, Nitnipa Soontorngun","doi":"10.1093/femsyr/foag021","DOIUrl":"10.1093/femsyr/foag021","url":null,"abstract":"Candida albicans biofilms present a major clinical challenge due to their resistance to conventional antifungal therapies. Sophorolipids (SLs), glycolipid biosurfactants produced by Starmerella riodocensis, have emerged as promising antifungal agents. This study evaluates SLs produced using soybean meal, a low-cost agro-industrial substrate, for antibiofilm activity and synergistic potential with amphotericin B (AmB) and itraconazole (ITZ), two widely used antifungals. Both crude and purified SLs reduced biofilm formation and metabolic activity, with purified SLs showing superior efficacy. SLs reduced the amount of AmB required to achieve up to 90% biofilm inhibition. SL-ITZ combinations also showed enhanced antibiofilm activity, indicating synergy across distinct antifungal classes. Given the amphiphilic nature of SLs and the central role of adhesion, hyphal development, and morphogenesis in biofilm formation, we hypothesized that SLs interfere with key virulence-associated processes. Accordingly, qRT-PCR analysis demonstrated significant downregulation of ALS3, HWP1, and CDC28 under hyphal-inducing conditions. Cytotoxicity assessment using SLs, representing a conservative evaluation, showed no significant toxicity to mammalian cells. Collectively, these findings highlight soybean meal-derived SLs as effective and safe adjuncts to conventional antifungal therapy for combating drug-resistant C. albicans biofilms.","PeriodicalId":12290,"journal":{"name":"FEMS yeast research","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13263898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0