Fish & shellfish immunology最新文献

{"title":"BSA-seq and transcriptome analysis identification candidate genes associated with Pseudomonas plecoglossicida resistance in the large yellow croaker (Larimichthys crocea)","authors":"Ting Ye ,&nbsp;Feng Liu ,&nbsp;Xiao Liang ,&nbsp;Dandan Guo ,&nbsp;Wei Zhan ,&nbsp;Guoer Shao ,&nbsp;Bao Lou","doi":"10.1016/j.fsi.2025.110533","DOIUrl":"10.1016/j.fsi.2025.110533","url":null,"abstract":"<div><div>Visceral white-nodule disease (VWND), caused by <em>Pseudomonas plecoglossicida</em>, poses a significant threat to the development of the <em>Larimichthys crocea</em> aquaculture industry. Breeding disease-resistant strains offers a potential solution, but the underlying mechanisms of disease resistance in <em>L. crocea</em> remain unclear, and relevant functional markers are still lack. In the present study, 243 full-sibling F₁ populations of <em>L. crocea</em> were infected with <em>P. plecoglossicida</em>. Whole-genome resequencing, bulk segregant analysis (BSA) mapping, and spleen transcriptome analysis were performed on two extreme phenotype pools, which defined by survival time (AT) and spleen bacterial load (BL) as disease-resistance indicators. Candidate genes were validated through infection experiments. A total of 5,576,771 SNPs were identified, and Δ(SNP-index), Euclidean distances (ED), and G-statistics were applied to locate two stable disease resistance-related QTLs on Chr 02 (2.6 Mb) and Chr 24 (4.9 Mb) with 99 % confidence, containing 278 candidate genes. Transcriptomic analysis revealed 4811 genes involved in the immune response, with 949 genes linked to <em>P. plecoglossicida</em> resistance variation in <em>L. crocea</em>. Integrated analysis identified 17 core immune gene, and subsequent validation confirmed that 6 genes (<em>Phf11</em>, <em>CYBB</em>, <em>PTK2B</em>, <em>STAT1</em>, <em>Adamts1</em>, and <em>NRP2</em>) play a consistent role in <em>L. crocea</em> resistance. Our findings highlight the utility of BSA-seq for genetic analysis of disease resistance and provide insights for molecular-assisted breeding in <em>L. crocea</em>.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110533"},"PeriodicalIF":4.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in vitro study of type I interferon and inflammatory markers induced by dsRNA and dsRNA-phytoglycogen in rainbow trout","authors":"Kristof Jenik ,&nbsp;Tania Rodríguez-Ramos ,&nbsp;Adebisi Sanyaolu ,&nbsp;Brian Dixon ,&nbsp;Stephanie DeWitte-Orr","doi":"10.1016/j.fsi.2025.110524","DOIUrl":"10.1016/j.fsi.2025.110524","url":null,"abstract":"<div><div>Understanding the induction patterns for rainbow trout type I interferon and inflammatory markers is essential for the development of new antipathogenic therapeutics and vaccines as well as enhancing aquaculture productivity and biosecurity. Type I interferons (ex<em>. ifn1</em>) and interferon stimulated genes (ex. <em>vig-3</em>) as well as inflammatory markers such as interferon gamma (<em>ifn</em>-γ) and interleukin 1beta (<em>il-1β</em>) all play a crucial role in protecting rainbow trout against pathogen infection. One strategy to better understand how fish defend themselves is by studying the effects of synthetic viral dsRNA analogues such as polyinosinic:polycytidylic acid (poly IC) on the fish innate immune response. The current work utilizes a phytoglycogen-based nanoparticle (Nanodendrix; NDx) to enhance the immunostimulatory effects of poly IC in three rainbow trout cell lines derived from monocyte/macrophages (RTS11), gonads (RTG-2) and gill (RTgill-W1). Variations in innate immune responses were observed between cell lines, between poly IC and poly IC + NDx treatment groups and between transcript, protein and antiviral response levels of study. The poly IC + NDx complex demonstrated prolonged immune stimulation up to 96h post-treatment and exhibited significant inhibition of infectious pancreatic necrosis virus (IPNV) replication. These findings highlight the potential of poly IC + NDx complexes as a novel antiviral therapeutic approach for future <em>in vitro</em> and <em>in vivo</em> studies.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110524"},"PeriodicalIF":4.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RBCK1 enhances antiviral response through promoting K63-linked polyubiquitination of IRF7 in Carassius auratus var. Pengze","authors":"Quanling Zhang ,&nbsp;Tingting Yu ,&nbsp;Zhongwei Wang ,&nbsp;Miaomiao Li ,&nbsp;Jingen Xu ,&nbsp;Xuefeng Liu ,&nbsp;Cheng Qian ,&nbsp;Zijia Yin ,&nbsp;Gang He ,&nbsp;Menglei Hu ,&nbsp;Binchao Zhang ,&nbsp;Jiwei Liu ,&nbsp;Zhixin Fu ,&nbsp;Shanghong Wang ,&nbsp;Xiaowen Xu ,&nbsp;Chengyu Hu","doi":"10.1016/j.fsi.2025.110532","DOIUrl":"10.1016/j.fsi.2025.110532","url":null,"abstract":"<div><div>Ubiquitination is a widely occurring reversible post-translational modification process in cells. RANBP2-type and C3HC4-type zinc finger-containing 1 (RBCK1) is a kind of E3 ubiquitin ligase. It regulates various biological functions, including protein degradation and kinase signaling pathways in mammals. However, the role of RBCK1 in the virus-induced innate immune response remains largely unknown, particularly in teleost. In this study, we demonstrated that the expression of <em>Carassius auratus</em> var Pengze RBCK1 (<em>Cap</em>RBCK1) was induced by grass carp reovirus (GCRV) and poly (I:C) stimulation in tissues and cells. <em>Cap</em>RBCK1 suppressed the replication of GCRV in cells. Overexpression of <em>Cap</em>RBCK1 activated IFN1 response, whereas knockdown of <em>Cap</em>RBCK1 inhibited this response. <em>Cap</em>RBCK1 is primarily localized in the cytoplasm, where it directly interacted with IRF7 and thereby increased the phosphorylation and dimerization of IRF7 in GCRV-infected cells. Furthermore, <em>Cap</em>RBCK1 catalyzed K63-linked ubiquitination of IRF7 at lysine 106. Finally, the mutant of <em>Cap</em>RBCK1 RING domain exhibited a significant impairment to IRF7 ubiquitination. So, the RING domain is essential for <em>Cap</em>RBCK1 enhancing IFN1 activation. This study provides, to our knowledge, some novel insights into the role of RBCK1 in innate immune response.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110532"},"PeriodicalIF":4.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary valine modulates hepatic immune responses in largemouth bass (Micropterus salmoides) against Aeromonas veronii infection: Integrated regulation of antioxidant defense, ER stress and autophagy","authors":"Ju Zhao ,&nbsp;Xiaoqing Li ,&nbsp;Tianran Zhang ,&nbsp;Haifeng Liu ,&nbsp;Quanquan Cao ,&nbsp;Jun Jiang","doi":"10.1016/j.fsi.2025.110531","DOIUrl":"10.1016/j.fsi.2025.110531","url":null,"abstract":"<div><div>Largemouth bass (<em>Micropterus salmoides</em>) is an economically important aquaculture species in China. <em>Aeromonas veronii</em> (<em>A. veronii</em>), a conditional pathogen, causes substantial economic losses in largemouth bass farming. Valine (Val), an essential amino acid for fish, plays a crucial role in the growth of largemouth bass; however, its effects on liver health under <em>A. veronii</em> infection remain unclear. This study investigated the influence of dietary Val on hepatic antioxidant capacity, inflammatory response, apoptosis, and autophagy in largemouth bass following <em>A. veronii</em> challenge. A total of 720 juveniles (37.99 ± 0.01 g) were fed six isonitrogenous diets with graded levels of Val (11.0–26.1 g/kg diet) for 11 weeks, and the fish were subjected to a 7 d challenge trial with <em>A. veronii</em>. Results showed that dietary Val supplementation (20.1 g/kg) effectively mitigated <em>A. veronii</em>-induced histopathological damage, notably attenuating both necrotic changes and inflammatory infiltration. Mechanistically, dietary Val enhanced hepatic antioxidant capacity by activating the Nrf2/Keap1 pathway, significantly increasing antioxidant enzyme activities (SOD1, CAT, GST, GPx, GR, GCLC) and reducing oxidative stress markers (ROS, MDA, PC) (<em>P</em> &lt; 0.05); Dietary Val attenuated apoptosis through downregulation of endoplasmic reticulum (ER) stress markers (GRP78, ATF6, IRE1, PERK, eIF2α, ATF4, CHOP) and modulation of Bcl2/Bax ratio (<em>P</em> &lt; 0.05); Dietary Val suppressed excessive autophagy as evidenced by decreased expression of key autophagy-related genes (ULK1, ATG9, BECN1, ATG7, ATG5, LC3) and proteins (<em>P</em> &lt; 0.05). Dietary Val regulated inflammatory responses by suppressing pro-inflammatory cytokines (TNF-α, IL-1β) while promoting anti-inflammatory factors (TGF-β2, IL-10). These findings demonstrate Val's multifaceted hepatoprotective effects through coordinated regulation of oxidative stress, apoptosis and autophagy pathways in <em>A. veronii</em>-infected largemouth bass.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110531"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The galectin family in large yellow croaker (Larimichthys crocea): Genome-wide identification, stress-responsive regulation to Pseudomonas plecoglossicida infection and hypoxia, and functional validation of LcGal-2a","authors":"Longdi Wang ,&nbsp;Xuan Tan ,&nbsp;Rumeng Zhai ,&nbsp;Jiaxin Song ,&nbsp;Xiumei Liu ,&nbsp;Xubo Wang","doi":"10.1016/j.fsi.2025.110527","DOIUrl":"10.1016/j.fsi.2025.110527","url":null,"abstract":"<div><div>Galectins are carbohydrate-binding proteins involved in many physiological functions, including immune response and cell migration. Although galectins have been studied in various fish species, their functional roles and expression patterns in large yellow croaker (<em>Larimichthys crocea</em>) remain poorly understood. In this study, 13 galectin genes (Lcgalectins) were identified using the whole genome database of large yellow croaker. Phylogenetic analyses as well as gene structure characterization of coding/non-coding regions, motif information, and gene locations revealed evolutionary conservation and highly conserved domains in galectins. Under acute hypoxia stress, multiple Lcgalectins displayed temporally and spatially dynamic expression patterns across different tissues, suggesting their potential involvement in hypoxia-response. Following <em>Pseudomonas plecoglossicida</em> infection, diverse Lcgalectins were significantly altered in the spleen of large yellow croaker, highlighting their potential participation in immune responses. Protein-protein interaction network analysis further revealed extensive interactions between protein family members. Among these, Lcgalectin-2a displayed significant expression changes following acute hypoxia and <em>P. plecoglossicida</em> infection, with protein-protein interaction analysis revealing its central network position. Based on these findings, LcGal-2a was selected for further functional study and was found to possess significant bacteriostatic properties. These results provided valuable insights into the immunological and stress-responsive roles of galectins in large yellow croaker, laying the groundwork for future studies on their molecular mechanisms and applications in aquaculture disease management.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110527"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal Isatidis Radix extract enhances growth, immunity, modifies immune-gene action and intestinal health in hybrid snakehead (Channa maculata ♀ × Channa argus ♂) against rhabdovirus","authors":"Shuzhan Fei ,&nbsp;Jiamin Kang ,&nbsp;Junhao Zhang ,&nbsp;Haiyang Liu ,&nbsp;Qing Luo ,&nbsp;Jian Zhao ,&nbsp;Mi Ou","doi":"10.1016/j.fsi.2025.110529","DOIUrl":"10.1016/j.fsi.2025.110529","url":null,"abstract":"<div><div><em>Isatidis Radix</em> is a traditional Chinese medicinal herb renowned for its broad-spectrum antimicrobial, antiviral, and anti-inflammatory properties. This study assessed the effects of <em>Isatidis Radix</em> extract on growth performance, non-specific immunity and intestinal health and disease resistance in hybrid snakehead (<em>Channa maculata ♀ × Channa argus ♂</em>). Fish were fed diets supplemented with <em>Isatidis Radix</em> extract at concentrations of 0 (C), 0.5 % (IRE0.5), 1.0 % (IRE1.0), 1.5 % (IRE1.5), and 2.0 % (IRE2.0) for 8 weeks. The results showed that with the increase of <em>Isatidis Radix</em> extract levels, the weight gain rate (WGR) and specific growth rate (SGR) significantly increased and reached the maximum at 1.5 %. The serum and liver activities of phosphatase (ACP), alkaline phosphatase (AKP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the <em>Isatidis Radix</em> extract supplemented group were significantly lower than those in the control group, demonstrating a dose-dependent decrease as the concentration increased. Antioxidant enzyme activities, such as superoxide dismutase (SOD) and catalase (CAT) in the IRE1.5 group were significantly elevated, whereas malondialdehyde (MDA) content were decreased. The IRE2.0 group demonstrated significant upregulation of intestinal antioxidant genes, including <em>sod</em>, <em>cat</em>, kelch-like ECH-associated protein 1 (<em>keap1</em>), and glutathione peroxidase (<em>gpx</em>), while the pro-inflammatory factors interleukin-1 beta (<em>il-1β</em>) and tumor necrosis factor-alpha (<em>tnf-α</em>) were significantly down-regulated. The 16S rRNA analysis indicated that the IRE1.0 group exhibited the highest Chao,the abundance of potentially pathogenic Proteobacteria was reduced, while the abundance of beneficial bacteria Acidobacteriota, <em>Staphylococcus</em>, and <em>Glutamicibacter</em> increased. The cumulative survival rate of hybrid snakeheads after <em>rhabdovirus</em> infection increased in a dose-dependent manner following the administration of <em>Isatidis Radix</em> extract in their diet. In conclusion, this study indicated that dietary <em>Isatidis Radix</em> extract (1 %–1.5 %) significantly improved the growth performance, antioxidant capacity and antiviral resistance of juvenile hybrid snakehead.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110529"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization and immune-modulatory roles of PPAR genes in Cynoglossus semilaevis: insights into inflammation regulation and host defense","authors":"Yishuai Li ,&nbsp;Mengyang Chang ,&nbsp;Suxu Tan ,&nbsp;Wenwen Wang ,&nbsp;Kunpeng Shi ,&nbsp;Shaoqing Zang ,&nbsp;Zhujun Li ,&nbsp;Jin Liu ,&nbsp;Zhenxia Sha","doi":"10.1016/j.fsi.2025.110528","DOIUrl":"10.1016/j.fsi.2025.110528","url":null,"abstract":"<div><div>Peroxisome proliferator-activated receptors (PPARs), members of the nuclear receptor superfamily, play essential roles in orchestrating metabolic processes and modulating innate immune responses. However, the roles of <em>PPAR</em> genes in <em>Cynoglossus semilaevis</em> remain largely unexplored. In this study, four <em>PPAR</em> genes were identified and categorized into three subfamilies based on genome-wide analysis. Physicochemical property analysis revealed that PPARγ is distinct from the other three isoforms, as it harbors a unique PPARγ_N superfamily domain and is exclusively localized in the nucleus. Comparative and evolutionary analyses demonstrated a high degree of conservation between <em>C. semilaevis</em> and other bony fish. The Ka/Ks ratio consistently remained below 1, suggesting strong purifying selection acting on <em>PPAR</em> genes. qRT-PCR analysis demonstrated a general downregulation of <em>PPAR</em>s in the early phase of <em>Vibrio anguillarum</em> infection across multiple tissues, potentially facilitating an inflammatory response against the pathogen. In the later stage, their expression increased, which may play a role in suppressing excessive inflammation. Functional assays demonstrated that overexpression of <em>pparαa</em>, <em>pparδ</em>, and <em>pparγ</em> suppressed LPS-induced exaggerated expression of inflammatory factors, suggesting their roles in negative regulation of excessive immune response. Moreover, protein-protein interaction (PPI) network analysis predicted candidate interactors, offering new avenues for further functional investigation. Collectively, the study provided a comprehensive characterization, expression, and immune function of <em>PPAR</em> genes in Chinese tongue sole, setting a solid foundation for further functional studies and practical usage in disease control.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110528"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis reveals the role of MAPK signaling pathway in IgM+ B cells against Pseudomonas plecoglossicida infection in large yellow croaker (Larimichthys crocea)","authors":"Zhuo Zhu ,&nbsp;Qiuxuan Chen ,&nbsp;Qiuhua Li ,&nbsp;Meiyan Wang ,&nbsp;Yihan Zhang ,&nbsp;Zhengwei Cui ,&nbsp;Yuan Shi ,&nbsp;Xinhua Chen","doi":"10.1016/j.fsi.2025.110526","DOIUrl":"10.1016/j.fsi.2025.110526","url":null,"abstract":"<div><div>The large yellow croaker (<em>Larimichthys crocea</em>), an economically important marine fish in China, often suffers serious losses due to visceral white nodule disease caused by <em>Pseudomonas plecoglossicida</em> infection. IgM⁺ B cells play critical roles in the defense against bacterial infection, yet their immune response and underlying mechanisms against the <em>P. plecoglossicida</em> infection in large yellow croaker remain poorly characterized. In this study, we used fluorescence-activated cell sorting (FACS) and RNA sequencing to profile the transcriptomes of head kidney IgM⁺ B cells at different time points after <em>P. plecoglossicida</em> infection. The results showed that <em>P. plecoglossicida</em> infection induced time-dependent transcriptomic changes in IgM⁺ B cells. Mfuzz analysis categorized genes into 9 different clusters based on their expression patterns. Notably, cluster 9, 8 and 1 exhibited specific upregulation at 1, 3, and 7 days post infection (dpi), respectively, while cluster 2 displayed a sustained upregulation pattern throughout infection. KEGG enrichment analysis revealed enhanced energy metabolism and proliferation ability of IgM⁺ B cells after infection. Furthermore, we found that the MAPK signaling pathway was rapidly activated after infection (enriched in cluster 9). qPCR results further confirmed the upregulation of MAPK signaling related genes (cacna1g, map2k1, map3k4, map3k10) at 1 dpi, suggesting its role in regulating the early immune response of B cells. Together, this study comprehensively analyzed the transcriptomic dynamics of IgM⁺ B cells after <em>P. plecoglossicida</em> infection, which would provide insights into the understanding of teleost B cell immunity and offer potential strategies for bacterial disease control in large yellow croaker aquaculture.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110526"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral infections affect the transcription of biological clock components in lymphoid organs and lymphoid-associated tissues of common carp (Cyprinus carpio L.)","authors":"Mikolaj Mazur ,&nbsp;Lukasz Pijanowski ,&nbsp;Mikolaj Adamek ,&nbsp;Marek Matras ,&nbsp;Krzysztof Rakus ,&nbsp;Magdalena Chadzinska","doi":"10.1016/j.fsi.2025.110525","DOIUrl":"10.1016/j.fsi.2025.110525","url":null,"abstract":"<div><div>The circadian clock is a mechanism that allows organisms to adapt to the changing environment in a diurnal manner. It receives external cues (time givers), which synchronize the internal circadian clock with the external environment. A crucial aspect of the circadian clock involves the positive and negative feedback loops of clock genes.</div><div>In the present study, we examined the diurnal changes in the expression of clock genes in the lymphoid organs (thymus, spleen, head kidney, trunk kidney), lymphoid-associated tissues (gills and gut), and peripheral blood leukocytes (PBLs) of common carp. Moreover, we investigated how the expression of these clock genes changes in these tissues/organs upon viral infections.</div><div>Our findings showed that clock genes are constitutively expressed in the lymphoid organs/tissues, gills, gut and PBLs of common carp, and that different light regimes (LD, DD, and LL) altered their expression patterns. We also demonstrated that the two studied viruses, cyprinid herpesvirus 3 (CyHV-3) and spring viremia of carp virus (SVCV), affect the expression of clock genes (<em>per1, per2</em>, <em>cry1,2</em>, <em>clock</em> and <em>bmal1,2</em>) in the head kidney, trunk kidney, gill, and skin of common carp under the LD regime.</div><div>Clock gene expression was negatively correlated with viral copy numbers.</div><div>Overall, this study shows that the immune system and biological clock are interconnected and suggests that proper lighting conditions are crucial for the well-being and functionality of the circadian clock in fish. This consideration is important both in experimental settings and especially in aquaculture.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110525"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of opioid growth factor receptor (OGFR) promotes wound healing in the freshwater pearl mussel (Hyriopsis schlegelii)","authors":"Kaixin Chen ,&nbsp;Kou Peng ,&nbsp;Hao Zhang ,&nbsp;Jiaqian Li ,&nbsp;Xinyue Zheng ,&nbsp;Chunfu Chen ,&nbsp;Xiaoying Zeng","doi":"10.1016/j.fsi.2025.110530","DOIUrl":"10.1016/j.fsi.2025.110530","url":null,"abstract":"<div><div><em>Hyriopsis schlegelii</em>, widely regarded as the most economically significant freshwater pearl mussel farmed in China, is known for producing high-quality pearls. The damage inflicted on the organism by pearl insertion is incalculable. Opioid growth factor receptor (OGFR), as a receptor for the opioid growth factor (OGF; [Met⁵]-enkephalin), plays essential roles in regulating cell proliferation and wound healing. Nonetheless, the role of OGFR in <em>H. schlegelii</em> wound healing remains poorly understood. In this paper, a cDNA of 3662 bp for the pearl mussel <em>H.schlegelii OGFR</em> (hereinafter referred to as <em>HsOGFR</em>) was obtained using the rapid amplification of cDNA ends (RACE) method. The complete cDNA sequence of <em>HsOGFR</em> contains a 2640 bp open reading frame (ORF) encoding 879 amino acids. The amino acid sequences of HsOGFR are 43–63 % similar to those of other OGFRs. The N-terminal of HsOGFR contains a conserved OGFR_N domain. Transcripts of <em>HsOGFR</em> were expressed in all tested tissues, with the highest expression observed in the hepatopancreas. Histological evaluation demonstrated that RNA interference-mediated <em>HsOGFR</em> knockdown (via dsRNAs) or pharmacological inhibition with naloxone significantly accelerated wound healing following the removal of mantle tissues in the mussels. Further qRT-PCR assays revealed that the mRNA expression of <em>HsOGFR</em> was down-regulated in mantle and haemocytes following wound treatment (<em>P</em> &lt; 0.05). Concomitantly, <em>HsCDKN1</em> and <em>HsCDKN3</em> expression was moderately downregulated at the incision site post-wounding (<em>P</em> &lt; 0.05). Following <em>HsOGFR</em> interference, the expression levels of <em>HsKi67</em>, <em>HsCCND</em>, <em>HsCDKL1</em> and <em>HsCDK6</em> were significantly upregulated (<em>P</em> &lt; 0.05) compared with the wound group. Conversely, <em>HsCDKN1</em> and <em>HsCDKN3</em> expression was further downregulated after <em>HsOGFR</em> blockade (<em>P</em> &lt; 0.05). Collectively, these findings demonstrate that HsOGFR, as a homolog of OGFRs, is significantly involved in tissue repair in mollusks, and might work through cell cycle related proteins above.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110530"},"PeriodicalIF":4.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}