Fish & shellfish immunology最新文献

{"title":"Comparison analysis of circulating hemocytes in decapod crustaceans","authors":"Yiyi Su ,&nbsp;Feng Yang ,&nbsp;Fang Li","doi":"10.1016/j.fsi.2024.109947","DOIUrl":"10.1016/j.fsi.2024.109947","url":null,"abstract":"<div><div>Hemocytes are the primary immune cells of crustaceans. Few comparison studies have been done among different crustaceans and some key parameters of circulating hemocytes have not been investigated. Here, we compared the circulating hemocytes in six decapod crustaceans, <em>Cherax quadrinatus</em>, <em>Procambarus clarkii</em>, <em>Penaeus vannamei</em>, <em>Penaeus monodon</em>, <em>Eriocheir sinensis</em>, and <em>Scylla paramamosain.</em> Although the hemocytes of different species vary in size, they share common morphological characteristics. Based on their morphological features, circulating hemocytes can be basically classified into granular cells (GCs), semi-granular cells (SGCs), and hyaline cells (HCs). In the six decapods analyzed in this study, the proportion of GCs varied from 10 % to 30 %. <em>P. vannamei</em>, <em>P. monodon</em>, and <em>P. clarkii</em> had fewer GCs in circulation than the other three species. Correspondingly, proliferation was detected only in a small portion of cells in <em>P. vannamei</em>, <em>P. monodon</em>, and <em>P. clarkii</em> under physical conditions. The hemocyte renewal rates for <em>P. clarkii</em>, <em>E</em>. <em>sinensis</em>, and <em>C</em>. <em>quadrinatus</em> were 6.1 %, 5.1 %, and 1.5 % per day, while no steady new hemocyte production was found in <em>S. paramamosain</em> within six days. These data give a general picture of the similarities and differences of circulating hemocytes in decapods and provide a base for an in-depth study of their immune system.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109947"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-glucan regulates the intestinal immunity of pearl gentian grouper via the nuclear factor kappa B signaling pathway","authors":"Fan Wang ,&nbsp;Jia Xu ,&nbsp;Chaoqun Hu ,&nbsp;Junxiang Lai ,&nbsp;Peihong Shen ,&nbsp;Yishan Lu ,&nbsp;Fajun Jiang","doi":"10.1016/j.fsi.2024.109941","DOIUrl":"10.1016/j.fsi.2024.109941","url":null,"abstract":"<div><div>The preceding study observed that yeast β-glucan supplementation enhanced intestinal health and augmented disease resistance in pearl gentian grouper (<em>Epinephelus lanceolatus</em>♂ × <em>Epinephelus fuscoguttatus</em>♀), which occurred concurrently with the activation of the nuclear factor kappa B (NFκB) signaling pathway. Thus, we hypothesized that β-glucan improves intestinal health in grouper by modulating the NFκB pathway. Accordingly, the present study examined the effects of NFκB pathway disruption using a specific inhibitor on the intestinal health of pearl gentian grouper that had been injected with β-glucan. The experimental groups were as follows, (1) CD group: PBS injected; (2) βG group: β-glucan injected at a dose of 80 mg/kg; (3) PDTC group: NFκB inhibitor PDTC injected at a dose of 30 mg/kg; (4) βG + PDTC group: a combination of β-glucan (80 mg/kg) and PDTC (30 mg/kg) injected together. The results demonstrated that β-glucan-induced increases in mRNA expression levels of NFκB inhibitor α (<em>iκbα</em>) and <em>p65</em>, the degradation and phosphorylation of IκBα, and the phosphorylation of NFκB p65 were significantly inhibited following NFκB inhibition using PDTC in the intestine of grouper. The PDTC injection resulted in a significant reduction in the β-glucan-induced increase in mucin levels. The β-glucan-induced elevation of alkaline phosphatase (AKP) activity, component 3 (C3) content, and inflammatory factors were significantly suppressed following NFκB inhibition. The βG + PDTC treatment resulted in a restoration of catalase (CAT) enzyme activity to the level observed in the CD treatment, while total antioxidant capacity (T-AOC) was decreased to the level of the βG treatment. The β-glucan-induced downregulation of caspase8 (<em>casp8</em>) was reversed following NFκB inhibition, as well as the mRNA levels of <em>casp3</em> and casp9 being elevated to a greater extent. In conclusion, the β-glucan-regulated intestinal immunity in grouper may be mediated by the NFκB pathway. Furthermore, the inhibitory effect of β-glucan on apoptosis and oxidative stress may not be related to the NFκB signaling pathway.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109941"},"PeriodicalIF":4.1,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic energetic adaptation of Atlantic salmon phagocytes to changes in carbon sources and exposure to PAMPs","authors":"Daniela Ortiz ,&nbsp;Francisco Guajardo ,&nbsp;Andrea Talamilla-Espinoza ,&nbsp;Francisca Vera-Tamargo ,&nbsp;Javiera Pérez-Valenzuela ,&nbsp;Madelaine Mejías ,&nbsp;Lucas Pino-Quezada ,&nbsp;Felipe Galdames-Contreras ,&nbsp;Dinka Mandakovic ,&nbsp;Jurij Wacyk ,&nbsp;Félix A. Urra ,&nbsp;Rodrigo Pulgar","doi":"10.1016/j.fsi.2024.109926","DOIUrl":"10.1016/j.fsi.2024.109926","url":null,"abstract":"<div><div>Phagocytic cells are pivotal for host homeostasis and infection defense, necessitating metabolic adaptations in glycolysis, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (OXPHOS). While mammalian phagocytes shift towards glycolysis and glutaminolysis during polarization, research on fish phagocyte metabolic reprogramming is limited. To address this, the Atlantic salmon phagocytic cell line, SHK-1, serves as a valuable model. Using the Seahorse XFe96 Flux Analyzer, this study compares SHK-1 bioenergetics under glucose-restricted (L-15 medium) and glucose-supplemented (PM) conditions, providing insights into metabolic characteristics and responses to <em>Piscirickettsia salmonis</em> bacterium Pathogen-associated molecular patterns (PAMPs). A standardized protocol for the study of real-time changes in the metabolism study of SHK-1 in PM and L-15 media, determining oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) is shown. Exhibiting metabolic adaptations, SHK-1 cells in the PM medium have higher basal and maximal OCR and spare capacity (SRC), while those grown in the L-15 medium favor OXPHOS, showing minimal glycolytic function. Despite metabolic differences, intracellular ATP levels are comparable, highlighting the metabolic plasticity and adaptability of SHK-1 cells to various carbon sources. Exposure to PAMPs from <em>Piscirickettsia salmonis</em> induces a metabolic shift, increasing glycolysis and OXPHOS, influencing ATP, lactate, glutamine, and glutamate levels. These findings highlight the role of mitochondrial bioenergetics and metabolic plasticity in salmon phagocytes, offering novel nutritional strategies for host-pathogen interventions based on energy metabolism.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109926"},"PeriodicalIF":4.1,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the functional properties of thioredoxin domain-containing protein 12 (TXNDC12): Antioxidant activity, immunological expression, and wound-healing effect in yellowtail clownfish (Amphiprion clarkii)","authors":"M.A.H. Dilshan ,&nbsp;W.K.M. Omeka ,&nbsp;H.M.V. Udayantha ,&nbsp;D.S. Liyanage ,&nbsp;D.C.G. Rodrigo ,&nbsp;W.A.D.L.R. Warnakula ,&nbsp;H.A.C.R. Hanchapola ,&nbsp;Y.K. Kodagoda ,&nbsp;G.A.N.P. Ganepola ,&nbsp;Jeongeun Kim ,&nbsp;Gaeun Kim ,&nbsp;Jihun Lee ,&nbsp;Taehyug Jeong ,&nbsp;Sukkyoung Lee ,&nbsp;Qiang Wan ,&nbsp;Jehee Lee","doi":"10.1016/j.fsi.2024.109939","DOIUrl":"10.1016/j.fsi.2024.109939","url":null,"abstract":"<div><div>Thioredoxin domain-containing protein 12 (TXNDC12) is a member of the thioredoxin-like superfamily that contributes to various thiol-dependent metabolic activities in all living organisms. In this research, the <em>TXNDC12</em> gene from yellowtail clownfish (<em>Amphiprion clarkii</em>) was structurally characterized using <em>in silico</em> tools, assessed for immunological expression, and evaluated for biological activity using recombinant protein and cellular overexpression. The deduced coding sequence of AcTXNDC12 comprised a 522-bp nucleotide, encoding 173 amino acids with a predicted molecular mass of 19.198 kDa. The AcTXNDC12 protein consists of a⁶⁶WCGAC⁷⁰ active motif and a¹⁷⁰GDEL¹⁷³ signature. The highest tissue-specific expression of <em>AcTXNDC12</em> was observed in the brain tissue, with significant modulation observed in the blood and gill tissues following stimulation of polyinosinic: polycytidylic acid, lipopolysaccharides (LPS), and <em>Vibrio harveyi.</em> In functional assays, recombinant AcTXNDC12 protein (rAcTXNDC12) showed insulin disulfide reduction activity, 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) decolorization antioxidant capacity, and ferric (Fe³⁺) reducing antioxidant potential. Additionally, a significant reduction in nitric oxide production was observed in AcTXNDC12-overexpressed RAW 264.7 cells upon LPS stimulation. Furthermore, genes associated with the regulation of oxidative stress, including nuclear factor erythroid 2-related factor 2 (<em>Nrf2</em>), catalase (<em>Cat</em>), peroxiredoxin 1 (<em>Prx1</em>), and ribonucleotide reductase catalytic subunit M1 (<em>Rrm1</em>) were significantly upregulated in fathead minnow cells overexpressing <em>AcTXNDC12</em> in response to H₂O₂ treatment. The scratch wound healing assay demonstrated tissue regeneration and cell proliferation ability upon AcTXNDC12 overexpression. Altogether, the current study elucidated the antioxidant activity, immunological importance, and wound-healing effect of the <em>AcTXNDC12</em> gene in yellowtail clownfish, providing valuable insights for advancing the aquaculture of <em>A. clarkii</em> fish.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109939"},"PeriodicalIF":4.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ocean warming on early development of the Mediterranean mussel Mytilus galloprovincialis: Effects on larval susceptibility to potential vibrio pathogens","authors":"Teresa Balbi ,&nbsp;Matteo Bozzo ,&nbsp;Manon Auguste ,&nbsp;Michele Montagna ,&nbsp;Angelica Miglioli ,&nbsp;Kévin Drouet ,&nbsp;Luigi Vezzulli ,&nbsp;Laura Canesi","doi":"10.1016/j.fsi.2024.109937","DOIUrl":"10.1016/j.fsi.2024.109937","url":null,"abstract":"<div><div>In a global change scenario, ocean warming and pathogen infection can occur simultaneously in coastal areas, threatening marine species. Data are shown on the impact of temperature on early larvae of the Mediterranean mussel <em>Mytilus galloprovincialis</em>. Increasing temperatures (18-20-22 °C) altered larval phenotypes at 48 hpf and affected gene expression from eggs to 24 and 48 hpf, with shell biogenesis related genes among the most affected. The effects of temperature on larval susceptibility to infection were evaluated using <em>Vibrio coralliilyticus</em>, a coral pathogen increasingly associated with bivalve mortalities, whose ecology is affected by global warming. Malformations and mortalities at 48 hpf were observed at higher temperature and <em>vibrio</em> concentrations, with interactive effects. In non-lethal conditions, interactions on gene expression at 24 and 48 hpf were also detected. Although temperature is the main environmental driver affecting <em>M. galloprovincialis</em> early larvae, warming may increase the susceptibility to <em>vibrio</em> infection, with consequences on mussel populations.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109937"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation with Akkermansia muciniphila improved intestinal barrier and immunity in zebrafish (Danio rerio)","authors":"Yawei Wang ,&nbsp;Zhenyi Huang ,&nbsp;Zewei Gui ,&nbsp;Boya Yang ,&nbsp;Fu You ,&nbsp;Guokun Yang ,&nbsp;Xindang Zhang ,&nbsp;Xulu Chang ,&nbsp;Xiaolin Meng","doi":"10.1016/j.fsi.2024.109935","DOIUrl":"10.1016/j.fsi.2024.109935","url":null,"abstract":"<div><div><em>Akkermansia muciniphila</em> (Akk), a second-generation probiotic known for its ability to regulate intestinal function in mammals, is not yet fully understood in the context of aquaculture. This study aims to investigate the effects of different forms of Akk on intestinal barrier function and immune response in zebrafish (<em>Danio rerio</em>) under high-fat diet conditions. The experimental groups included a control group, a high-fat diet group, an Akk group, and a group receiving various concentrations of pasteurized <em>Akkermansia muciniphila</em> (P-Akk) along with a high-fat diet. Evaluation methods included histological examination with hematoxylin and eosin staining, ultrastructural analysis using transmission electron microscopy, real-time fluorescence quantitative analysis, and transcriptome sequencing technology. The results showed that both the Akk and P-Akk groups exhibited a significant increase in villi number and length compared to the high-fat group. Furthermore the expression levels of <em>claudin</em>, <em>claudin-2</em>, <em>occludin A</em>, <em>occludin B</em>, and other genes were significantly upregulated, while the expression levels of intestinal proinflammatory factors genes and proteins were significantly downregulated. Compared to the high-fat group, the Akk group showed a more complete and well-preserved nucleus, mitochondria, and tight junction structures. Additionally, the morphology of intestinal epithelial microvilli in the medium and high concentration Akk group was complete and dense. The expressions of <em>tlr2</em> and <em>nf-κb</em> were upregulated, while the expressions of myd88 and nod2 were downregulated in the medium- and high-concentration Akk groups. Akk may improve immune dysfunction in high-fat fed zebrafish through the TLR2/NF-κB signaling pathway, which requires further study. Transcriptome analysis revealed significant upregulation of the immune-related gene <em>pigr</em>, significant downregulation of <em>stat3</em>, and significant upregulation of the intercellular adhesion molecule <em>f11r</em>. In conclusion, dietary Akk supplementation alleviated intestinal barrier damage and immune dysfunction in high-fat zebrafish. This study provides important insights into the potential use of Akk in fish and lays the foundation for further studies on its role in fish immunity.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109935"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Clostridium butyricum metabolites mitigated the disturbances in growth, immune response and gut health status of Ctenopharyngodon idella subjected to high cottonseed and rapeseed meal diet","authors":"Shao Wang ,&nbsp;Minglang Cai ,&nbsp;Yan Wang ,&nbsp;Lei Zhong ,&nbsp;Yi Hu ,&nbsp;Guihong Fu","doi":"10.1016/j.fsi.2024.109934","DOIUrl":"10.1016/j.fsi.2024.109934","url":null,"abstract":"<div><div>Cottonseed meal and rapeseed meal exhibit a potential for fishmeal substitute in grass carp feed, while their excessive use contribute to growth decline and weakening immunity of aquatic animals. <em>Clostridium butyricum</em> metabolites (CBM) was recognized as a functional additive due to its antioxidant properties and maintenance of intestinal microbiota balance. CBM was added to a high of cottonseed and rapeseed meal diet to determine its effects on growth, immunity, and intestinal microbiota alterations of grass carp (<em>Ctenopharyngodon idella</em>) over 56 days. Eight hundred grass carp (mean weight, around 50 g) were randomized to five treatments and fed with the basic diet (CON), CBM0 diet (28 % cottonseed and 27 % rapeseed meal), and CBM diets (CBM0.5, CBM1, and CBM2, namely CBM0 diet supplemented with 500, 1000, and 2000 mg kg⁻¹ CBM). The results indicated that compared to CBM0, The ingestion of 1000 mg kg⁻¹ CBM diet by grass carp significantly promoted growth as measured by intestinal lipase activity, villus height, and muscle thickness. Moreover, accompanied by a decrease in intestine MDA content, and enhance antioxidant capacity by activating Keap1/Nrf2 signaling pathway to increase enzyme activities (SOD, CAT and T-AOC) and corresponding gene expression (<em>mnsod</em>, <em>cat</em>, <em>gsto</em> and <em>gpx1</em>) in the intestine of grass crap fed CBM1 diet. The dietary CBM1 diet increased serum levels of C3 and IgM, increased ACP activity and expression of the corresponding anti-inflammatory factors (<em>tgf-β1</em> and <em>il-15</em>), and suppressed the expression of pro-inflammatory factors (<em>tnf-α</em> and <em>il-12β</em>), resulting in enhanced immunity. The dietary CBM1 diet up-regulates gene expression of tight junction proteins (<em>zo-1</em>, <em>occludin</em>, <em>occludin7a</em> and <em>occludin-c</em>), coupled with the decreases in DAO and D-lactate contents, implying that the decreased mucosal permeability could be observed in the gut. The dietary CBM1 diet largely altered the intestinal microbial community, especially reducing the relative abundance of intestinal pathogenic bacteria (<em>Streptococcus</em> and <em>Actinomyces</em>). And it significantly increased the content of short-chain fatty acids (acetic acid, butyric acid, isobutyric acid, propionic acid and isovaleric acid). Taken above, dietary CBM supplementation improved growth in grass carp and attenuated the intestinal oxidative stress, inflammation and microflora dysbacteriosis caused by high proportions of cottonseed and rapeseed meal diets.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109934"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low temperature alleviated the adverse effects of simulated transport stress on the intestinal health in Chinese soft-shelled turtle Pelodiscus sinensis","authors":"Jiaxiang Luo,&nbsp;Jintao Gao,&nbsp;Haoran Song,&nbsp;Zhiyin Mo,&nbsp;Binquan Hong,&nbsp;Leyan Zhu,&nbsp;Wei Song,&nbsp;Guoying Qian,&nbsp;Caiyan Li","doi":"10.1016/j.fsi.2024.109936","DOIUrl":"10.1016/j.fsi.2024.109936","url":null,"abstract":"<div><div>Transport stress always poses a threat to aquatic animals. Transportation under low temperatures was often used to relieve transport stress in practical production of Chinese soft-shelled turtle <em>Pelodiscus sinensis</em>, but their effect on the turtle's intestinal barrier remains unclear. In this study, <em>P</em>. <em>sinensis</em> (initial weight 200 ± 20 g) were exposed to simulated transport stress for 12 h at control (30 °C) and low (20 °C) temperature, and then recovery for 24 h, and each treatment had 4 replicates with each replicate containing 4 turtles. The results showed that transportation induced obvious morphological and histological damages in intestinal villus, with a down-regulated expression of the tight junction related genes. Besides turtles in transport group showed an oxidative stress in intestine, which stimulated a physiological detoxification response together with apoptosis. Low temperature transport plays a mitigative effect on the transport stress of turtle intestine via relieved stress response. Specifically, the intestinal villus/crypt (V/C) ratio and the expression of tight junction genes in the low-temperature group were significantly higher compared to the control temperature group, while stress response parameters such as intestinal cortisol levels and <em>hsp</em> expression were significantly lower in the low-temperature group. Additionally, low temperature alleviated oxidative damage and apoptosis caused by transport stress relative to the control temperature group. However, the protective effect of low temperature on <em>P</em>. <em>sinensis</em> intestine was limited, especially after the temperature recovery stage. Overall, the findings of the present study demonstrated that transport stress would induce the disruption of intestinal integrity and oxidative damage, also activated the mucosal immunity and antioxidant enzyme system response of turtles. It was also suggested that low temperature could alleviate the adverse effects of transport stress on intestinal integrity through modulation of oxidative status and apoptosis, whereas much less impact after temperature recovery.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109936"},"PeriodicalIF":4.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenomethionine alleviates Aeromonas hydrophila-induced oxidative stress and ferroptosis via the Nrf2/HO1/GPX4 pathway in grass carp","authors":"Penghui Yu ,&nbsp;Lei Su ,&nbsp;Bo Li ,&nbsp;Jianguo Su ,&nbsp;Gailing Yuan","doi":"10.1016/j.fsi.2024.109927","DOIUrl":"10.1016/j.fsi.2024.109927","url":null,"abstract":"<div><div><em>Aeromonas hydrophila</em> infection is a severe, acute, and life-threatening disease affecting grass carp (<em>Ctenopharyngodon idella</em>) in aquaculture. Ferroptosis is a novel form of cell death characterized by the accumulation of free iron and harmful lipid peroxides within cells. While selenomethionine (Se-Met) is known to effectively inhibit ferroptosis and alleviate cell damage, its ability to counteract oxidative stress and ferroptosis induced by <em>A. hydrophila</em> remains unclear. The objective of this study is to reveal the possible mechanism behind the ferroptosis phenomenon during <em>A. hydrophila</em> infection. We established a macrophage model of <em>A. hydrophila</em> invasion to monitor the dynamic changes in iron metabolism markers to evaluate the correlation between ferroptotic stress and <em>A. hydrophila</em> infection. <em>A. hydrophila</em> infection induces cytotoxicity and mitochondrial membrane damage via ferroptosis. This damage is attributed to the accumulation of lipid peroxides due to intracellular ferrous ion overload and glutathione depletion. Supplementation of Se-Met reduced mitochondrial damage, enhanced antioxidant enzyme activity and reduced ferroptosis by activating the Nrf2/HO1/GPX4 axis. These findings provide new insights into the regulatory mechanisms of <em>A. hydrophila</em>-induced ferroptosis in teleosts and suggest that targeted inhibition of ferroptosis may offer a novel therapeutic strategy for managing <em>A. hydrophila</em> infections.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109927"},"PeriodicalIF":4.1,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-375 modulates neutrophil chemotaxis via targeting Cathepsin B in zebrafish","authors":"Decheng Wang ,&nbsp;Tianqi Wang ,&nbsp;Daniel Kim ,&nbsp;Shelly Tan ,&nbsp;Sheng Liu ,&nbsp;Jun Wan ,&nbsp;Qing Deng","doi":"10.1016/j.fsi.2024.109933","DOIUrl":"10.1016/j.fsi.2024.109933","url":null,"abstract":"<div><div>Neutrophils are crucial for defense against numerous infections, and their migration and activations are tightly regulated to prevent collateral tissue damage. We previously performed a neutrophil-specific miRNA overexpression screening and identified several microRNAs, including miR-375, as potent modulators for neutrophil activity. Overexpression of miR-375 decreases neutrophil motility and migration in zebrafish and human neutrophil-like cells. We screened the genes downregulated by miR-375 in zebrafish neutrophils and identified that Cathepsin B (Ctsba) is required for neutrophil motility and chemotaxis upon tail wounding and bacterial infection. Pharmacological inhibition or neutrophil-specific knockout of <em>ctsba</em> significantly decreased the neutrophil chemotaxis in zebrafish and survival upon systemic bacterial infection. Notably, Ctsba knockdown in human neutrophil-like cells also resulted in reduced chemotaxis. Inhibiting integrin receptor function using RGDS rescued the neutrophil migration defects and susceptibility to systemic infection in zebrafish with either miR-375 overexpression or <em>ctsba</em> knockout. Our results demonstrate that miR-375 and its target Ctsba modulate neutrophil activity during tissue injury and bacterial infection <em>in vivo</em>, providing novel insights into neutrophil biology and the overall inflammation process.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"154 ","pages":"Article 109933"},"PeriodicalIF":4.1,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}