Fish & shellfish immunology最新文献

{"title":"Survival, serum biochemical parameters, hepatic antioxidant status, and gene expression of three Nile tilapia strains under pathogenic Streptococcus agalactiae challenge.","authors":"Jinglin Zhu, Dayu Li, Wei Xiao, Jie Yu, Binglin Chen, Zhiying Zou, Hong Yang","doi":"10.1016/j.fsi.2024.110030","DOIUrl":"https://doi.org/10.1016/j.fsi.2024.110030","url":null,"abstract":"<p><p>Streptococcosis is the leading bacterial disease impacting Nile tilapia (Oreochromis niloticus) and causes substantial economic losses in China. This study assessed the resistance and tolerance of three Nile tilapia (88, 99, and NG) to Streptococcus agalactiae infection. Survival rates were monitored, and samples were collected from blood, liver, and spleen at 0, 1, 2, 5, 7, and 14 days post-infection. Serum biochemical parameters, hepatic antioxidant enzymes, and the expression of related antioxidant and immune genes were measured. Results showed that strain 88 had superior resistance, with the highest survival rate, reduced liver damage, and lower alanine aminotransferase and alkaline phosphatase levels at 1 and 5 days post-infection. This strain also had higher serum cholesterol and triglyceride levels between days 5 and 14. Antioxidant activities increased in all strains post-infection, with strain 88 showing significantly higher superoxide dismutase (SOD) activity and elevated catalase (CAT) and SOD gene expressions; this indicated enhanced control of reactive oxygen species and reduced tissue damage. Additionally, strain 88 exhibited lower levels of inflammatory markers (TNF-α, IL-1β, IL-10) with higher IgM levels and superior MHC-II expression during early infection, which demonstrated a strong immune response. These results indicate that strain 88 is a promising candidate for selective breeding to improve streptococcosis resistance in tilapia.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110030"},"PeriodicalIF":4.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation mechanism of oxidative status, immunity and apoptosis induced by hypoxia and heat exposure via PI3K/Akt signaling pathway in Megalobrama amblycephala.","authors":"Kang Chen, Yihui Jia, Zheng He, Peiyu Xie, Hong Liu, Zexia Gao, Huanling Wang","doi":"10.1016/j.fsi.2024.110027","DOIUrl":"10.1016/j.fsi.2024.110027","url":null,"abstract":"<p><p>Megalobrama amblycephala, a main herbivorous fish with notable economic benefits in China, often faces serious challenges to its survival and growth due to hypoxia and heat caused by factors such as global warming and intensive aquaculture. To evaluate the combined effects of these stressors, we performed a two-factor crossover test to assess the impacts of simultaneous exposure to hypoxia (2 mg/L) and heat (35 °C) on oxidative stress, immunity and apoptosis in M. amblycephala. These results showed that hypoxia and heat exposure significantly enhanced the expression of oxygen-sensing and heat shock protein (HSP) genes, hypoxia inducible factor 1α (Hif-1α), HIF-prolyl hydroxylase-2 (phd2) and factor inhibiting Hif-1 (fih-1), as well as hsp70 and hsp90α. Furthermore, M. amblycephala suffering from hypoxia and heat exposure exhibited several changes in liver tissues, with the most severe lesions and up-regulation of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) observed in those subjected to simultaneous exposure. Moreover, the combined hypoxia and heat exposure initially triggered an increase in the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and catalase (CAT), and glutathione (GSH) contents, followed by a reduction, and the accumulation of malondialdehyde (MDA), which induced oxidative stress. This was accompanied by an increase and subsequent reduction in the contents of alkaline phosphatase (AKP), acid phosphatase (ACP), complement component 3 (C3) and C4, immunoglobulin M (IgM), and interferon-γ (IFN-γ) leading to immunosuppression. Additionally, hypoxia and heat exposure up-regulated the expression of antioxidant enzyme genes (nrf2, cu/zn-sod, mn-sod cat, ho-1, pi3k and gpx-1a), inflammatory genes (interleukin il-1β, il-8 and tnf-α), immunity effectors (igm and lyz), as well as apoptosis genes (casp3, casp8, casp9 and p53) and activated p-Akt/Akt, suggesting apoptosis may be linked with oxidative stress and inflammation and mediated through the PI3K/Akt signaling pathway. In short, the combined hypoxia and heat exposure disrupted homoeostasis in M. amblycephala, with a more pronounced detrimental effect than exposure to either stressor alone These results will contribute to understanding the mechanism of combined exposure to hypoxia and heat in fish and provide a fundamental base for fisheries management.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110027"},"PeriodicalIF":4.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel C-type lectin, perlucin, from the small abalone, Haliotis diversicolor involved in the innate immune defense against Vibrio harveyi infection.","authors":"Tuo Yao, Zhengxin Tong, Jie Lu, Shengli Fu, Changhong Cheng, Lingtong Ye","doi":"10.1016/j.fsi.2024.110029","DOIUrl":"https://doi.org/10.1016/j.fsi.2024.110029","url":null,"abstract":"<p><p>C-type lectins (CTLs), a member of pattern recognition receptors, play an important role in the innate immunity by recognizing invading microorganisms. In this study, a novel perlucin gene (designated as HdPer 3), a typical CTLs was cloned and characterized from the small abalone Haliotis diversicolor. The open reading frame of HdPer 3 was 471 bp, encoding a protein of 156 amino acids that included a single carbohydrate-recognition domain. HdPer 3 was widely expressed in all tested tissues and developmental stage. HdPer 3 expression was significantly up-regulated after Vibrio harveyi infection, suggesting that HdPer 3 was activated in response to bacterial infection. The encapsulation ability of hemocytes could be significantly enhanced by the recombinant protein HdPer 3 (rHdPer 3). To understand the regulation mechanism of the HdPer 3, HdPer 3 was silenced in vivo by RNAi. Knocking down HdPer 3 decreased the hemocytes phagocytosis. Meanwhile, knocking down HdPer 3 can reduce the expression of 2 phagocytosis-related genes (Rab and Dynamin), TNF-α, and 2 MAPK pathway-related genes (MAPK-X1 and Ras) after V. harveyi infection. Moreover, HdPer 3 interference could increase the bacterial load in the hemolymph and the mortality of abalones after V. harveyi infection. All these results suggested that HdPer 3 played a crucial role in the defense against V. harveyi infection by recognizing bacterial pathogens and activating the expression of immune-related genes.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110029"},"PeriodicalIF":4.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-intestinal microbiota interactions in Edwardsiella piscicida-induced lethal enteritis in big-belly seahorses: novel insights into the role of Carbohydrate-Active enzymes and host transcriptional responses.","authors":"Longwu Jia, Lele Zhang, Hongwei Yang, Lin Li, Shiyi Zheng, Yicong Ma, Yuanyuan Xue, Jingyi Zhang, Mingzhu Li, Xiaolei Su, Kai Wang","doi":"10.1016/j.fsi.2024.110024","DOIUrl":"10.1016/j.fsi.2024.110024","url":null,"abstract":"<p><p>Edwardsiella piscicida-induced lethal enteritis is a major threat to the sustainable development of seahorse aquaculture. The roles of Carbohydrate-Active enzymes (CAZymes) in interactions between the pathogen and the host are poorly understood. In this study, we found that 22 key CAZymes encoded by E. piscicida might involve in the coordination of five key stages of infection. Specifically, during the motility, adherence, and invasion stages, 10 CAZymes, including CE4, PL8, and CBM48, may significantly increase the activities of Lipid metabolism-associated pathways of the intestinal microbiota (P < 0.01), facilitating pathogen invasion of the host intestinal epithelium. During the replication stage, 11 CAZymes, including GH20, GT4, and GH3, may significantly increase activities of pathways associated with Carbohydrate metabolism (P < 0.01) to promote replication and proliferation of the pathogen. And for avoiding host defenses, GH2 and GH1 may enhance activities of both Carbohydrate and Amino acid metabolic pathways (P < 0.01), facilitating infection and immune evasion. Conjoint analysis showed that E. piscicida might mainly rely on Carbohydrate metabolism for infection, while the host might activate Amino acid metabolic pathways for self-defense. In addition, expressions of 10 key genes, Aldh9a1b, Aoc1, Tpi1b, PCK1, Ldha, Me1, Gla, Cel.2, Ugdh, and Mao, were significantly altered (P < 0.01) and may be used for characterizing host responses to E. piscicida infection. Activities of both Glycolysis/Gluconeogenesis and Tryptophan metabolism were found oppositely changed (P < 0.01) between pathogen and host, respectively, representing the primary focuses of the competition. Overall, this study provides new insights into E. piscicida-mediated intestinal enteritis in fish for the first time from the perspective of CAZymes, as well as a theoretical reference for the prevention and control of these diseases in the aquaculture of seahorses and other fish.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110024"},"PeriodicalIF":4.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel perlucin with immune regulatory functions protects Litopenaeus vannamei against Vibrio parahaemolyticus infection.","authors":"Zi-Ang Wang, Linwei Yang, Zexu Zhao, Shaoping Weng, Jianguo He, Xiaopeng Xu","doi":"10.1016/j.fsi.2024.110028","DOIUrl":"10.1016/j.fsi.2024.110028","url":null,"abstract":"<p><p>C-type lectins (CTLs), a class of carbohydrate-recognizing glycoproteins, play a vital role in immune response against bacterial infection. Vibrio parahaemolyticus is a major bacterial pathogen in shrimp, causing huge economic losses to shrimp farming. The role of the CTL family in anti-V. parahaemolyticus immunity requires further exploration. In this study, a novel CTL named Perlucin with immune regulatory functions was characterized from Litopenaeus vannamei. Perlucin was highly expressed in the muscle and hepatopancreas of healthy L. vannamei. The mRNA levels of Perlucin were significantly upregulated after LPS stimulation, and V. parahaemolyticus, Staphylococcus aureus and Aspergillus niger infections. Silencing of Perlucin by injection of specific dsRNA decreased the survival rate of V. parahaemolyticus-infected shrimp and increased the bacterial load of V. parahaemolyticus in tissues, while injection of recombinant Perlucin protein had the opposite effect. Moreover, silencing of Perlucin significantly affected the expression of multiple immune-related genes, including immune signaling components and downstream effector genes, suggesting that Perlucin is involved in immune regulation. This suggests that perlucin plays a crucial role in regulating humoral immune response against V. parahaemolyticus infection in shrimp.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110028"},"PeriodicalIF":4.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predominant role of IgM in grouper (Epinephelus coioides) mucosal defense against ectoparasitic protozoan infection","authors":"Zequan Mo ,&nbsp;Huajian Lin ,&nbsp;Xueli Lai ,&nbsp;Pengbo Dan ,&nbsp;Huicheng Wu ,&nbsp;Xiaochun Luo ,&nbsp;Xueming Dan ,&nbsp;Yanwei Li","doi":"10.1016/j.fsi.2024.110023","DOIUrl":"10.1016/j.fsi.2024.110023","url":null,"abstract":"<div><div>The skin mucosa of fish is exposed to significant challenges from infectious disease agents due to continuous exposure to the aqueous environment. Interestingly, bony fish have evolved to express a unique IgT, which is absent in terrestrials, that appears to play a predominant role in the mucosal-associated lymphoid tissue of the rainbow trout. Nevertheless, in other IgT-producing fish, it is unclear whether IgM or IgT is primarily responsible for protection against infections of cutaneous tissue. Here, we show that grouper IgM appears quickly within the skin following challenge by the marine parasite, <em>Crytopcaryon irritans</em>. These IgM-class antibodies may arise from local proliferating antibody secreting cells or may infiltrate tissue from the serum in dimer polymer form. Based on details of IgM functional responses, we conclude that grouper IgM plays a predominant role in defense against <em>C. irritans</em>.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"155 ","pages":"Article 110023"},"PeriodicalIF":4.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics and transcriptomics analysis reveals the enhancement of growth, anti-oxidative stress and immunity by (-)-epigallocatechin-3-gallate in Litopenaeus vannamei","authors":"Xuanyi Yang ,&nbsp;Mei Liu ,&nbsp;Keyong Jiang ,&nbsp;Baojie Wang ,&nbsp;Lei Wang","doi":"10.1016/j.fsi.2024.110025","DOIUrl":"10.1016/j.fsi.2024.110025","url":null,"abstract":"<div><div>(−)-epigallocatechin-3-gallate (EGCG), the main active component of tea polyphenols, has been less studied in <em>Litopenaeus vannamei</em>. Therefore, the potential benefits of EGCG on <em>L. vannamei</em> were explored in this experiment. <em>L. vannamei</em> were fed diets containing EGCG (0, 0.5, and 1.0 g/kg). At the end of the 60-day farming experiment, metabolomics, transcriptomics, quantitative real-time PCR assays, and tissue sections were used to assess and explore the effects of EGCG on growth, antioxidant capacity, and immunity. It was found that the best growth and genes expressions related to antioxidant, anti-stress, apoptosis, and immunity were observed in the group with 0.5 g/kg EGCG. The analysis of hepatopancreatic metabolomics and transcriptomics results revealed bumper differential metabolites and differentially expressed genes (DEGs) associated with nutrient metabolism, digestion, immunity, and environmental adaptation. Many metabolites with anti-inflammatory, antibacterial, antiviral, and antioxidant activities have been identified. A large number of DEGs were enriched in apoptosis, Rap1 signaling pathway, HIF-1 signaling pathway, hippo signaling pathway, chemokine signaling pathway, and signaling pathways related to amino acid and lipid metabolism. In conclusion, dietary 0.5 g/kg EGCG was beneficial for growth, resistance to oxidative stress, and immunity in <em>L. vannamei</em>. The improvement of the properties was attributed to the modulation of signaling pathways, metabolites abundance and genes expressions related to the above properties by EGCG.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"155 ","pages":"Article 110025"},"PeriodicalIF":4.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel antimicrobial peptide CpAMP identified from Chinese horseshoe crab, Tachypleus tridentatus.","authors":"Weifeng Wang, Sisi Wei, Xiaoyong Xie, Baoying Guo, Hong Liu, Huanling Wang","doi":"10.1016/j.fsi.2024.110026","DOIUrl":"https://doi.org/10.1016/j.fsi.2024.110026","url":null,"abstract":"<p><p>Antibacterial peptide (AMP) is a crucial component of the innate immune system in most organism, play an important role in host defense processes. Many of these peptides have broad antimicrobial activity against Gram-negative, -positive bacteria and fungi. In the present study, a novel AMP named CpAMP was identified using transcriptome analysis in Chinese horseshoe crab. The preprotein of CpAMP consists of a signal peptide (21 aa) and a mature peptide (47 aa) enriched by cysteine. And the putative mature peptide CpAMP was 4.95 kDa with a theoretical isoelectric point (pI) of 8.73. The mature CpAMP showed α-helix and irregularly curled structure in the cys-stabilized region. CpAMP exhibited a broad spectrum of antimicrobial activity against Gram-negative, -positive bacteria and antibiotic-resistant strains. In addition, CpAMP can significantly increase the survival rate of zebrafish infected with bacteria. Due to its broad-spectrum antibacterial activity and the sensitivity of drug-resistant strains, CpAMP could be used as a new type of drug for inhibition of pathogenic microorganisms or as an immune enhancer in animals.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110026"},"PeriodicalIF":4.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel goldfish orthotopic xenograft model of hepatocellular carcinoma developed to evaluate antitumor drug efficacy","authors":"Fenghua Zhang ,&nbsp;Zhixin Qu ,&nbsp;Jing Zeng ,&nbsp;Lanxin Yu ,&nbsp;Laifeng Zeng ,&nbsp;Xianmei Li","doi":"10.1016/j.fsi.2024.109998","DOIUrl":"10.1016/j.fsi.2024.109998","url":null,"abstract":"<div><div>Tumor xenograft animal models play a crucial role in hepatocellular carcinoma (HCC) research. Mice xenograft models are time consuming, laborious and expensive while zebrafish tumor xenograft models are cost-effective and effortless. However, the development of orthotopic xenograft models for HCC in zebrafish embryos has been challenging due to the small size of zebrafish livers. In this study, we utilized 7-day-old goldfish embryos as hosts and successfully established an orthotopic xenograft model of HCC in goldfish livers. Through injecting fluorescence labeled HCC cells into the liver of goldfish, we could visualize the proliferation and migration of tumor cells <em>in vivo</em>. In addition, we found that the temperature of 36 °C was better for tumor cell survival in goldfish larvae compared to 28 °C, assessed by EdU and TUNEL assays. Moreover, macrophage infiltration in the goldfish liver could be evaluated by neutral red staining. Finally, we evaluated the efficacy of the targeted therapy drug Sorafenib and the traditional Chinese medicine, Huaier granules, alone or in combination in the goldfish HCC orthotopic xenograft model. We found that the combination therapy showed the best efficacy against HCC cells in terms of macrophage infiltration, polarization as well as tumor cells proliferation, metastasis and apoptosis. In conclusion, the proposed goldfish HCC orthotopic xenograft model opens new avenues for HCC related research, including evaluation of tumor progression, cell interactions in the immune microenvironment, drug efficacy, and screening of anti-tumor drugs.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"155 ","pages":"Article 109998"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antibacterial defence role of β-defensin in the seahorse testis.","authors":"Wei Huang, Wanghong Xiao, Geng Qin, Zijian Lu, Xiaoqian Peng, Ying Liu, Qiang Lin, Jinhui Sun","doi":"10.1016/j.fsi.2024.110022","DOIUrl":"10.1016/j.fsi.2024.110022","url":null,"abstract":"<p><p>Seahorses represent the only known group of animals with male pregnancy. Seahorses have small testis that produce a limited quantity of sperm. To date, the response of this immune-privileged organ to pathogenic infections has not been reported. β-defensin (BD) is an important innate immune defence factor against pathogens in vertebrate testis. To elucidate its immunoprotection in seahorse testis, we identified the Hippocampus erectus β-defensin (HeBD) sequence in its genome via phylogenetic tree and protein-sequence structure analysis. Gene-expression analysis showed that HeBD was highly expressed in the seahorse testis and was significantly upregulated after bacterial infection, indicating that HeBD expression was related to testicular immune responses. Furthermore, antibacterial activity testing demonstrated that the mature HeBD peptide exhibited broad-spectrum aggregation activity but only moderate antibacterial activity. We found that the mature HeBD mature significantly neutralised bacterial endotoxin activity. In conclusion, our results imply that HeBD serves an immunoprotective role in seahorse testis.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110022"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}