Fish & shellfish immunology最新文献

{"title":"Transcriptome analysis reveals the mechanism of cortisol through GR regulating the expression of inflammatory cytokines in Siberian sturgeon (Acipenser baerii) after LPS treatment in vitro.","authors":"Peng Tang, Yinqiu Chen, Defang Chen, Hao Zhu, Shaotong Dai, Jiawen Zhou, Xin Zhang, Xiaoli Huang, Ping Ouyang, Yi Geng, Zhiqiong Li","doi":"10.1016/j.fsi.2025.110262","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110262","url":null,"abstract":"<p><p>Cortisol can impact the transcription of downstream inflammation and immune-related genes via the glucocorticoid receptor (GR), thereby influencing the immune response and maintaining the homeostatic balance of the host. However, there is a lack of research on the mechanisms by which cortisol affects the immune response of Siberian sturgeon (Acipenser baerii) through GR. In this study, an anti-inflammatory state of Siberian sturgeon was established by the combined treatment of head kidney (HK) leukocytes with LPS + cortisol. Subsequently, the inflammation-related genes of the AbGR antagonistic group (LPS + cortisol + RU-486) and the AbGR non-antagonistic group (LPS + cortisol) were compared by qRT-PCR and high-throughput sequencing methods. Furthermore, an AP-1 agonist ASLAN003 was used to detect the regulatory effect of the AP-1 gene on inflammatory cytokines. The results showed that cortisol downregulated the expressions of il-1β, il-6, il-8, tnf-α, and il-17c that were induced by LPS, while simultaneously promoting the expressions of tgf-β1. Moreover, this pattern was reversed by adding RU486. When analyzing the differentially expressed genes in the transcriptome sequencing of HK leukocytes in AbGR antagonistic group, 261 significantly down-regulated genes and 194 significantly up-regulated genes were annotated. Furthermore, 26 differentially expressed genes related to inflammation in AbGR antagonistic group were enriched, and the key nuclear transcription factor AP-1 for regulating inflammation function of AbGR was selected based on the enrichment factor and p-value for subsequent research. In the HK leukocytes after cortisol + ASLAN003 treatment, the expressions of fosl1 and jund that were induced by ASLAN003 were significantly down-regulated after cortisol treatment. In this situation, the expressions of il-10 and tgf-β1 were significantly increased, and the expressions of tnf-α and cox-2 were significantly decreased after cortisol treatment. Therefore, this study demonstrated that cortisol inhibits the expression of AP-1 through GR in Siberian sturgeon, and then regulates the generation of the inflammatory response.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110262"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy enhances the antibacterial response in Macrobrachium rosenbergii by modulating cellular metabolism and immune pathways.","authors":"Feifei Wang, Yang Liu, Jing Wen, Aiping Tan, Yuting Deng, Ling Wang, Hua Gong, Yingtiao Lai, Zhibin Huang, Fei Zhao","doi":"10.1016/j.fsi.2025.110258","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110258","url":null,"abstract":"<p><p>Autophagy plays a crucial role in innate and adaptive immunity against invading microorganisms. However, the mechanism underlying autophagy in Macrobrachium rosenbergii remains largely unknown. Here, we demonstrate that Aeromonas hydrophila activates autophagy in M. rosenbergii, according to western blot, qRT-PCR, and transmission electron microscopy observations. Rapamycin treatment to activate autophagy in M. rosenbergii followed by stimulation with A. hydrophila significantly decreased the A. hydrophila OmpA copy number in the gills of M. rosenbergii. Furthermore, high-throughput RNA-seq analysis of M. rosenbergii gills treated with rapamycin revealed 1684 upregulated and 1500 downregulated differentially expressed genes (DEGs), most of which regulate metabolic pathways. A comprehensive joint analysis of the two transcriptomic databases for A. hydrophila infection and rapamycin treatment identified 15 upregulated and 25 downregulated DEGs, respectively. These genes enhance the immune defense of M. rosenbergii by negatively regulating metabolic pathways and promoting immune pathways. Our results provide a theoretical basis for further exploration of the antibacterial mechanism of M. rosenbergii.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110258"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of immunological effects of two DNA vaccines against Nocardia seriolae in hybrid snakehead.","authors":"Yiming Wen, Guoquan Chen, Yuhao Li, Deyu Ning, Wanna Sirimanapong, Liqun Xia","doi":"10.1016/j.fsi.2025.110233","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110233","url":null,"abstract":"<p><p>Although DNA vaccines hold significant potential, their practical application in aquaculture remains limited. In both mammals and teleost fish, B cells, which recognize antigens and produce antibodies, play an important role in immunity. In this study, B-cell epitopes capable of inducing fish immunity from IS701 family transposase (IS701) and molybdopterin-dependent oxidoreductase (Mol) proteins of Nocardia seriolae were screened. pcDNA-IS701 and pcDNA-Mol recombinant plasmids were constructed. The results showed that two proteins possessed multiple B-cell epitopes, and both pcDNA-IS701 and pcDNA-Mol induced innate immunity and specific antibody responses, along with increased mRNA expression levels of genes encoding humoral (MHCIIα and CD4) and cell-mediated (MHCIα and CD8α) immunity. In addition, both pcDNA-IS701 and pcDNA-Mol strengthened the protection against N. seriolae infection, with immune protection rates of 45.06% for IS701 and 61.04% for Mol, respectively. In conclusion, pcDNA-IS701 and pcDNA-Mol are candidate DNA vaccines for nocardiosis in fish.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110233"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of a gill cell line from turbot (Scophthalmus maximus).","authors":"Jiaqi Wang, Xiang Shi, Zhihong Gong, Songlin Chen, Guobin Hu","doi":"10.1016/j.fsi.2025.110265","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110265","url":null,"abstract":"<p><p>Turbot (Scophthalmus maximus) is a main breeding marine fish species in northern China, but its aquaculture industry is seriously threatened by bacterial diseases. The turbot cell line will provide effective tools for the study of turbot immunity and basic biology. In this study, a continuous passaged Scophthalmus maximus gill (SMG) cell line was established through primary culture and subculture, and the optimal culture conditions were determined. The culture medium consists only of a basal medium L-15, fetal bovine serum (FBS) and antibiotics without supplementing growth factors and other additives. Its turbot-derivation was verified by chromosomal analysis and 18S rRNA sequencing. We also confirmed that pEGFP-C1 plasmid could transfect SMG cells. After lipopolysaccharide (LPS) stimulation, the transcriptome of SMG cells changed. The results of gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed that the differentially expressed genes were significantly enriched in immune-related signaling pathways such as Toll-like receptor signaling pathway and RIG-I-like receptor signaling pathway. Taken together, we established an easy-to-culture cell line from turbot gill, which provides a convenient tool for studying turbot diseases and immune mechanisms.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110265"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The structural characteristics and expression characteristics of C1S in response to GCRV infection in grass carp.","authors":"Yuling Wei, Zhao Lv, Zongjun Du, Tiaoyi Xiao","doi":"10.1016/j.fsi.2025.110264","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110264","url":null,"abstract":"<p><p>The complement system, a critical component of innate immunity in fish, plays a pivotal role in the defense against Grass Carp Reovirus (GCRV) infection in grass carp. This study explores the structural characteristics of C1S, a crucial molecule in the classical pathway of the complement system, and its involvement in the response to GCRV infection. We found that the grass carp C1S gene comprises six domains similar to those in mammals: two CUB (Complement C1r/C1s, Uegf, Bmp1) domains, two CCP (Complement control protein) domains, one EGFCA (Calcium-binding epidermal growth factor) domain, and one Tryp_SPc (Trypsin-like serine protease) domain, albeit without chromosomal collinearity to humans. Comparative analysis revealed that the identity and similarity of this gene with those in other species range from 30.6-89.4% and 30.7-89.7%, respectively. Phylogenetic analysis positioned C1S in close relation with R. klamathensis and D. rerio. Tissue expression profiles in both healthy and GCRV-infected grass carp indicated primary expression of C1S in the liver, with expression peaks at day 7 post-infection in the liver and spleen, and at day 5 in the kidney. Functional assays demonstrated that C1S activates the complement system via cleavage of complement component 3 (C3) into C3b, further inhibiting GCRV replication and upregulating antiviral genes IFN1, IRF3, and IRF7. These findings elucidate the mechanism by which the complement system mediates resistance to GCRV infection in grass carp, offering a substantial theoretical foundation for further research.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110264"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal effects on offspring immunity in fish.","authors":"Jie Ma, Kenneth D Cain","doi":"10.1016/j.fsi.2025.110261","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110261","url":null,"abstract":"<p><p>The aquaculture industry faces many challenges, particularly concerning disease-related mortality during early life stages. Disease impacts at this stage can disrupt seed stock availability and potentially affect industry supply chains. Enhancing immunocompetence in aquaculture species is crucial for sustainable and cost-effective production, with potential benefits including increased survival and reduced dependence on therapeutics such as antibiotics. Maternal immunity involving the transfer of immune factors from adult female broodstock to eggs and/or embryos can play a critical role in protecting vulnerable offspring against pathogens until their immune system becomes immunocompetent. This review summarizes the current understanding of maternal immunity in fish and provides insights into the factors influencing its impact on offspring of different fish species and their immune responses. In specific cases, maternal immunity can be targeted and enhanced to offer practical applications for aquaculture disease management and enhanced production. Understanding and optimizing maternal transfer of immunity in aquaculture holds significant potential for improving fish health and reducing disease impact.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110261"},"PeriodicalIF":4.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary evidence of a life stage specific antibody response to Cardicola spp. (Trematoda: Aporocotylidae) in ranched Southern bluefin tuna, Thunnus maccoyii","authors":"Maree Widdicombe ,&nbsp;Melissa Carabott ,&nbsp;Cecilia Power ,&nbsp;Emma Wanicek ,&nbsp;Daryl L. Evans ,&nbsp;Paul A. Ramsland ,&nbsp;Barbara F. Nowak ,&nbsp;Nathan J. Bott","doi":"10.1016/j.fsi.2025.110259","DOIUrl":"10.1016/j.fsi.2025.110259","url":null,"abstract":"<div><div><em>Cardicola forsteri</em> (Trematoda: Aporocotylidae) is a significant pathogen for the Australian Southern bluefin tuna (SBT, <em>Thunnus maccoyii</em>) aquaculture industry infecting up to 100 % of fish. This study investigated the relationship between the SBT <em>C. forsteri</em> antibody response and <em>Cardicola</em> spp. infection during an extended ranching period. SBT were sampled at 11 time points during commercial harvest in Port Lincoln, South Australia ranging from 13 to 40 weeks post treatment (PT) with praziquantel. <em>C. forsteri</em> specific serum antibodies were measured using an enzyme linked immunosorbent assay (ELISA) and <em>Cardicola</em> spp. infection was quantified using adult fluke counts from SBT heart and quantitative polymerase chain reaction (qPCR) of <em>C. forsteri</em> or <em>C</em>. <em>orientalis</em> ITS-2 rDNA in SBT gills. <em>C. forsteri</em> specific IgM was significantly higher in SBT positive for <em>C. forsteri</em> ITS-2 rDNA compared to SBT that were negative. <em>C. forsteri</em> specific antibody levels remained elevated throughout the study duration and were significantly higher at week 30 and week 40 PT than week 17 PT. A significant negative correlation between adult fluke infection intensity and <em>C. forsteri</em> specific IgM was identified at week 25 PT and a significant positive correlation was identified at week 40 for <em>C. forsteri</em> ITS-2 rDNA infection intensity and <em>C. forsteri</em> specific IgM. A positive correlation was identified between <em>C. forsteri</em> specific IgM and <em>C. forsteri</em> ITS-2 rDNA infection intensity from 4 to 5 weeks earlier. <em>Cardicola</em> spp. infection intensity peaked at 25 weeks PT before significantly decreasing for the remainder of the study despite 100 % infection prevalence of <em>C. forsteri</em> ITS-2 rDNA from SBT gills.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"161 ","pages":"Article 110259"},"PeriodicalIF":4.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The E3 ubiquitin ligase RNF182 regulates the induction of innate immune response against GCRV by mediating the ubiquitination of RIG-I in grass carp (Ctenopharyngodon idella) and rare minnow (Gobiocypris rarus).","authors":"Yusheng Lin, Haohao Feng, Yuxuan Wang, Shuai Liu, Pengcheng Hu, Jing Wang, Hong Cao","doi":"10.1016/j.fsi.2025.110244","DOIUrl":"https://doi.org/10.1016/j.fsi.2025.110244","url":null,"abstract":"<p><p>Innate immunity is the first line of antiviral or antimicrobial defence for the host. A cytoplasmic viral RNA sensor, which is known as retinoic acid-inducible gene 1 (RIG-I), makes a vital impact on the production of type I interferons (IFN) and eliminating RNA virus. This study indicated that E3 ubiquitin ligase RING finger protein 182 (RNF182) inhibited the antiviral activity of type I IFN in grass carp reovirus (GCRV)-infected cells by directly interplaying with RIG-I. The CiE3RNF182 cDNA encode a polypeptide of 158 amino acids. Cellular distribution analysis results suggested that cytoplasm was the main site of CiE3RNF182 location. Real-time quantitative PCR showed universal expression of CiE3RNF182 in all investigated tissues, with extremely high expression in liver. During virus infection, the CiE3RNF182 associates with the CiRIG-I and then induces the Lys-33-linked ubiquitin to the Lys33 residues of CiRIG-I to trigger its degradation, causing the inhibition of CiRIG-I downstream signalling. Furthermore, we obtained CRISPR/Cas9-mediated generation of E3RNF182-null rare minnows, finding that E3RNF182 deletion facilitates the survival ratio of GCRV-infected rare minnows. Additionally, the E3RNF182^-/- rare minnows exhibited significantly lower relative copy number of GCRV compared to the wild-type group. In summary, our findings demonstrate the function of E3 ligase in controlling the anti-GCRV innate immunity through RIG-I in fish.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110244"},"PeriodicalIF":4.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different molecular responses of Mytilus mantle to lipopolysaccharide and peptidoglycan challenges","authors":"Zilin Yang ,&nbsp;Pingling Cao ,&nbsp;Wenhui Xiao ,&nbsp;Fang Song ,&nbsp;Xiaoshan Wu ,&nbsp;Xiaolin Zhang ,&nbsp;Jianyu He ,&nbsp;Isabella Buttino ,&nbsp;Xiaojun Yan ,&nbsp;Zhi Liao","doi":"10.1016/j.fsi.2025.110246","DOIUrl":"10.1016/j.fsi.2025.110246","url":null,"abstract":"<div><div><em>Mytilus</em> live in water as sessile filter feeders, and the mantle tissue plays an important role in their immune defense. However, the overall knowledge of the immunity of this tissue remains limited. Peptidoglycan (PGN) and lipopolysaccharide (LPS) are the most representative microbe-associated molecular patterns (MAMPs) that play roles in the immune stimulation of host cells. In the present study, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)-based metabolomic analysis was performed to understand the precise regulatory mechanism at the molecular level in the <em>Mytilus</em> mantle in response to PGN and LPS stress. Moreover, the antioxidant ability and free amino acid composition of the mantle, and the antimicrobial activities of mantle mucus were evaluated. Our results revealed that LPS and PGN stresses had different effects on the mantle's free amino acid composition and antioxidant ability, and the mantle mucus' antimicrobial activity. Both PGN and LPS stress-induced alterations in amino acids, phospholipids, fatty acids, nucleotides, and their derivatives in the mantle. PGN injection activated the amino acid-related metabolism, and inhibited the lipid-related metabolisms in the mantle, while LPS injection activated the amino acid-related metabolisms and inhibited the arachidonic acid metabolism in the mantle compared to that in the control group. In addition, activation of the mTOR and FoxO signaling pathways and inhibition of lipid-related metabolism were observed in PGN <em>vs</em>. LPS. In addition, PGN injection induced the upregulation of fosfomycin and deoxynojirimycin in the mantle compared to LPS injection. Our study highlights the different responses at the metabolomic level of the mussel mantle to different MAMPs and the potential application of metabolites that specifically respond to PGN and LPS challenges in mussels as biomarkers.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"161 ","pages":"Article 110246"},"PeriodicalIF":4.1,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel role of PvUCP4 in Penaeus vannamei in response to Vibrio alginolyticus challenge","authors":"Zhengxinyu Liu ,&nbsp;Caoyuan Han ,&nbsp;FuHui Geng ,&nbsp;XiaoTong Huang ,&nbsp;BeiBei Dong ,&nbsp;Yu Zhang ,&nbsp;Weiguo Qian ,&nbsp;Qingjian Liang","doi":"10.1016/j.fsi.2025.110245","DOIUrl":"10.1016/j.fsi.2025.110245","url":null,"abstract":"<div><div>Mitochondria are the energy production and metabolic centers of cells. About 90 % of reactive oxygen species come from mitochondria. Uncoupling proteins (UCPs) have a protective effect against oxidative stress in mitochondria. But this regulatory mechanism remains poorly understood in crustaceans. Here, we investigated the mechanism of <em>Pv</em>UCP4 under <em>vibrio alginolyticus</em> challenge. Transcriptome analysis revealed that energy metabolism is a key pathway in the shrimp's immune response. Interestingly, <em>Pv</em>UCP4, a mitochondrial uncoupling protein, was found to be inhibited, indicating its potential involvement in the shrimp's resistance to <em>V. alginolyticus</em>. Silencing <em>Pv</em>UCP4 upregulates antioxidant enzyme gene expression, including SOD, CAT, and GPX. However, pretreatment with the ROS scavenger N-acetylcysteine revealed that silencing <em>Pv</em>UCP4 had no effect on the expression of antioxidant genes. Pretreatment with LPS, a bacterial cell wall component, can rescue partial inhibition of DJ-1/NF-κB signaling pathway related genes caused by overexpression of <em>Pv</em>UCP4. In addition, inhibiting <em>Pv</em>UCP4 increased the expression of apoptosis-related genes and induced apoptosis. Ultimately, silencing <em>Pv</em>UCP4 reduced the survival rate of shrimp under <em>V. alginolyticus</em> stress. Collectively, these findings suggest that <em>Pv</em>UCP4 is neither dependent on the antioxidant enzyme system for its antioxidant effects nor on the negative feedback regulation of the DJ-1 pathway.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"161 ","pages":"Article 110245"},"PeriodicalIF":4.1,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}