Fish & shellfish immunology最新文献

{"title":"Corrigendum to \"Soybean glycinin impaired immune function and caused inflammation associated with PKC-ζ/NF-κb and mTORC1 signaling in the intestine of juvenile grass carp (Ctenopharyngodon idella)\" [Fish and Shellfish Immunol. 106 (2020) 393-403].","authors":"Ya-Lin Zhang, Xu-Dong Duan, Lin Feng, Wei-Dan Jiang, Pei Wu, Yang Liu, Sheng-Yao Kuang, Ling Tang, Xiao-Qiu Zhou","doi":"10.1016/j.fsi.2024.110040","DOIUrl":"10.1016/j.fsi.2024.110040","url":null,"abstract":"","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110040"},"PeriodicalIF":4.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular cloning and functional characterization of Caspase-8 in goldfish (Carassius auratus L.).","authors":"Ziqi Ban, Xinyuan Yue, Kejing Huang, Lu Yuan, Suming Zhou, Jianhu Jiang, Chenjie Fei, Jiasong Xie","doi":"10.1016/j.fsi.2024.110090","DOIUrl":"10.1016/j.fsi.2024.110090","url":null,"abstract":"<p><p>Apoptosis, a form of programmed cell death, is essential for maintaining homeostasis within the internal environment. Caspase-8, an initiator caspase, plays a pivotal role in activating the caspase cascade during the apoptotic process. This study cloned and expressed Caspase-8 from goldfish, aimed to investigate the role of Caspase-8 in the immune response of fish to bacterial infections, specifically those caused by Aeromonas hydrophila. The Gf-Casp8 gene consists of 1425 base pairs, encoding a protein of 474 amino acids with a molecular weight of 54.55 kDa. Quantitative real-time PCR analysis revealed that Gf-Casp8 is highly expressed in the spleen and kidney, with lower expression levels in the muscle and heart. Moreover, Gf-Casp8 expression was significantly upregulated in kidney leukocytes following stimulation with A. hydrophila and LPS. Post-immunization, both mRNA and protein levels of Gf-Casp8 in the kidney were significantly increased. Subcellular localization analysis indicated that Gf-Casp8 is localized in both the cytoplasm and nucleus. In addition, flow cytometry analysis demonstrated that overexpression of Gf-Casp8 significantly enhances apoptosis in HEK293T cells. These results highlight the critical function of Gf-Casp8 in modulating apoptosis and antibacterial immune responses in goldfish.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110090"},"PeriodicalIF":4.1,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses of mRNA and circular RNA reveal dietary supplementation with freeze-dried Lactiplantibacillus plantarum primes immune memory of Whiteleg shrimp (Penaeus vannamei) against pathogens.","authors":"John Paul Matthew Domingo Guzman, Reiko Nozaki, Mikio Aoki, Hiroshi Kuwahara, Kazuki Mikata, Keiichiro Koiwai, Hidehiro Kondo, Ikuo Hirono","doi":"10.1016/j.fsi.2024.110091","DOIUrl":"10.1016/j.fsi.2024.110091","url":null,"abstract":"<p><p>The lack of a classical adaptive immunity renders the development of disease control and prevention measures in shrimp challenging. In this study, the concept of trained immunity was exploited in the development of a feed supplement. Penaeus vannamei shrimp was fed with feed supplemented with freeze-dried whole culture of Lactiplantibacillus plantarum (FD-LAB) for 15 days. RNA sequencing using Illumina platform was performed on the gill and stomach tissues collected at specific time points during the feeding period (0^th day, 8th day, 15th day). Differentially-expressed genes (DEGs) previously reported to have innate immunity- and immune memory-related functions were selected for validation. Additionally, the differential expression of putatively immune-related circular RNAs (DECs) were also explored as these noncoding regulatory RNAs may also influence host immunity. Challenge tests with either the acute hepatopancreatic necrosis disease-causing strain Vibrio parahaemolyticus D6 or White Spot Syndrome Virus (WSSV) were conducted. Transcriptome analyses showed that FD-LAB supplementation resulted to DEGs and DECs related to pathogen recognition, antimicrobial peptides, transcription regulation, and immune memory. Challenge tests performed immediately after 15 days and 8 days of feeding showed protection on P. vannamei by FD-LAB against bacterial and viral pathogens. Increase in survival rates were also observed upon challenge with both pathogens 7 days and 14 days after last intake of FD-LAB, indicating trained immunity in shrimp. Our study highlighted the effects of FD-LAB on the innate immunity and immune memory of P. vannamei against bacterial and viral pathogens. These findings emphasize the possibility of immunostimulants inducing lasting enhanced immunity against infections despite the lack of a classical adaptive immunity in shrimp.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110091"},"PeriodicalIF":4.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"The benefits of astaxanthin-rich microalgal powder on growth, health, and disease resistance against Fusarium solani in Pacific white shrimp\" [Fish Shellfish Immunol. 156 (2025) 110059].","authors":"El-Sayed Hemdan Eissa, Basma M Hendam, Hagar Sedeek Dighiesh, Heba E Abd Elnabi, Salah El-Sayed Sakr, Hoda Kabary, Afaf N Abdel Rahman, Moaheda E H Eissa, Norhan H Ahmed","doi":"10.1016/j.fsi.2024.110083","DOIUrl":"https://doi.org/10.1016/j.fsi.2024.110083","url":null,"abstract":"","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110083"},"PeriodicalIF":4.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of tumor necrosis factor receptor-associated factor 2 (TRAF2) in red-spotted grouper (Epinephelus akaara): In vivo and in vitro investigation of its role in the regulation of antiviral immunity and cell death.","authors":"E M T Tharanga, Kishanthini Nadarajapillai, W A D L R Warnakula, Gaeun Kim, Chaehyeon Lim, Hyerim Yang, J D H E Jayasinghe, Jeganathan Tharshan Jeyakanesh, D M K P Sirisena, U P E Arachchi, Qiang Wan, Jehee Lee","doi":"10.1016/j.fsi.2024.110089","DOIUrl":"10.1016/j.fsi.2024.110089","url":null,"abstract":"<p><p>Tumor necrosis factor receptor-associated factor 2 (TRAF2) is a key adaptor molecule in tumor necrosis factor receptor signaling complexes, facilitating downstream immune-related signaling cascades. This study aimed to elucidate its function in teleost fish by characterizing the TRAF2 homolog of the red-spotted grouper (Epinephelus akaara, EaTraf2). The open reading frame of EaTraf2 encodes a putative protein of 520 amino acids, containing several characteristic domains of TRAF2. These structural features of EaTraf2 are conserved across diverse organisms, with a relatively higher sequence identity to TRAF2 orthologs from other bony fish. Transcriptional analysis demonstrated that EaTraf2 was ubiquitously expressed in all examined tissues, with the highest level observed in blood. Upon immune challenge, EaTraf2 expression significantly increased in the early stages of stimulation in both blood and spleen. Subcellular localization analysis revealed that EaTraf2 is predominantly localized in the cytoplasm. Overexpression of EaTraf2 in fathead minnow (FHM) cells resulted in elevated levels of interferon and inflammation-associated genes following viral hemorrhagic septicemia virus (VHSV) infection, along with reduced viral gene expression. This provided compelling evidence that EaTraf2 possesses antiviral properties. Furthermore, EaTraf2 demonstrated the ability to promote cell death induced by oxidative stress. Additionally, luciferase reporter assays revealed that EaTraf2 activates the NF-κB signaling pathway upon poly(I:C) stimulation and the Jun N-terminal kinase (JNK) signaling pathway in response to H₂O₂ treatment. Overall, our study elucidated the role of EaTraf2 in regulating innate immune responses and mediating stress-induced cell death. These findings enhance our understanding of TRAF2 in fish and may contribute to improved health management strategies in finfish aquaculture.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110089"},"PeriodicalIF":4.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell transcriptome sequencing analysis of physiological and immune profiling of crucian carp (Carassius auratus) gills.","authors":"Zhi-Guang Hou, Meng-Chao Xing, Jia-Xing Luo, Yi-Huan Xu, Li-Han Zhang, Xiao-Wei Gao, Jiang-Jiang Wang, Fazhan Hanafiah, Waiho Khor, Xin Hao, Xin Zhao, Cheng-Bin Wu","doi":"10.1016/j.fsi.2024.110087","DOIUrl":"10.1016/j.fsi.2024.110087","url":null,"abstract":"<p><p>Gills are the main respiratory organs of fish and bear important physiological and immunological functions, but the functional heterogeneity of interlamellar cell mass (ILCM) at the single-cell level has rarely been reported. Here, we identified 19 cell types from the gills of crucian carp (Carassius auratus) by single-cell RNA sequencing (scRNA-seq) in combination with histological analysis. We annotated ILCM and analyzed its functional heterogeneity at the single-cell level for the first time. Functional enrichment analysis and cell cycle analysis identified ILCM as a type of metabolically active cells in a state of constant proliferation, and identified the major pathways responsible for ILCM immunoregulation. Histological analysis revealed the morphology and positional relationships of 6 cell types. Meanwhile, the gene regulatory network of ILCM was established through weighted gene co-expression network analysis (WGCNA), and one transcription factor and five hub genes related to immunoregulation were identified. We found that pyroptosis might be an important pathway responsible for the immune response of ILCM. Our findings provide an insight into the physiological and immune functions of gills and ILCM at the single-cell level and lay a solid foundation for further exploration of the molecular mechanism of ILCM immunity functions.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110087"},"PeriodicalIF":4.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of transcriptome responses in blood cells of Atlantic salmon infected by three genotypes of Piscine orthoreovirus.","authors":"Thomais Tsoulia, Arvind Ym Sundaram, Marit M Amundsen, Espen Rimstad, Øystein Wessel, Jorunn B Jørgensen, Maria K Dahle","doi":"10.1016/j.fsi.2024.110088","DOIUrl":"10.1016/j.fsi.2024.110088","url":null,"abstract":"<p><p>Piscine orthoreovirus (PRV) infection is common in aquaculture of salmonids. The three known PRV genotypes (PRV-1-3) have host species specificity and cause different diseases, but all infect and replicate in red blood cells (RBCs) in early infection phase. PRV-1 is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar), PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon (Oncorhynchus kisutch), while PRV-3 induces HSMI-like disease in farmed rainbow trout (Oncorhynchus mykiss). PRV-3 can also infect A. salmon without causing clinical disease and has been shown to cross-protect against PRV-1 infection and HSMI, while PRV-2 or inactivated adjuvanted PRV-1 vaccine only partially reduced HSMI pathologic changes. In the present work, we studied the transcriptional responses in blood cells of A. salmon two- and five-weeks post infection with PRV-1, PRV-2, PRV-3, or post injection with inactivated PRV-1 vaccine. PRV-1 and PRV-3 replicated well in A. salmon blood cells, and both induced the typical innate antiviral responses triggered by dsRNA viruses. Two weeks post infection, PRV-3 triggered stronger antiviral responses than PRV-1, despite their similar viral RNA replication levels, but after five weeks the induced responses were close to equal. PRV-2 and the InPRV-1 vaccine did not trigger the same typical antiviral responses as the replicating PRV-1 and PRV-3 genotypes, but induced genes involved in membrane trafficking and signaling pathways that may regulate physiological functions. These findings propose that the protection mediated by PRV-3 against a secondary infection by PRV-1 occur due to a potent and early activation of the same type of innate immune responses. The difference in the timing of antiviral responses may give PRV-1 an evolutionary edge, facilitating its dissemination to A. salmon heart, a critical step for HSMI development.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110088"},"PeriodicalIF":4.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating serum proteinogram methodologies for the diagnosis of inflammation in fish: Acute and chronic patterns in gilthead seabream (Sparus aurata) injected with λ-carrageenan.","authors":"Jose Carlos Campos-Sánchez, María Ángeles Esteban, Francisco A Guardiola","doi":"10.1016/j.fsi.2024.110084","DOIUrl":"10.1016/j.fsi.2024.110084","url":null,"abstract":"<p><p>Proteinogram is a semiquantitative method specially used in clinic to separate the serum proteins from patients for use in the diagnosis of diseases. However, this methodology has only been applied very recently with this approach in farmed fish. Thus, the aim of this study was to explore the changes in the serum proteinogram of gilthead seabream (Sparus aurata), after triggering an acute or chronic sterile inflammation. For this, two experiments were carried out: i) Acute inflammation experiment: seabream specimens were injected intramuscularly with 50 μL of λ-carrageenan (0.5 mg fish^-1) or buffer (control) and blood samples were collected at 3, 6 and 24 h post-injection; ii) Chronic inflammation experiment: specimens were injected at 0, 7 and 14 days with 500, 250 and 250 μL of λ-carrageenan, respectively (20 mg fish^-1) or buffer, and blood samples were collected at 15 days post-injection. In both cases, serum was obtained and processed by electropherograms and HPLC-mass spectrometry. Results of electropherograms of control fish revealed four major proteins of 19.5, 76.3, 104.4, and 156.7 kDa in the serum. These four proteins were correlated with apolipoprotein A-II (II (the counterpart of mammalian albumin, Apo fraction), serotransferrin (β fraction), inter-α-trypsin inhibitor heavy chain H3-like (α1 fraction) and α-2-macroglobulin-like (α2 fraction) according to the results obtained with HPLC-mass spectrometry. In a statistical view (p < 0.05), no variations were detected in the four major serum protein bands between the control and the acutely inflamed groups. However, in chronically inflamed fish, the Apo fraction decreased statistically compared to the control group. In contrast, the α1 and α2 fractions were statistically increased in the serum of fish sampled 15 days after λ-carrageenan injection, compared to those found in the control fish. α1 and α2 protein fractions are recognized indicators of inflammation in mammals. Consequently, our study presents a novel method for assessing both acute and chronic λ-carrageenan-induced sterile inflammation in gilthead seabream, which could be applicable to other marine species for diagnostic purposes.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110084"},"PeriodicalIF":4.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, characterization of Bacillus subtilis and Bacillus amyloliquefaciens and validation of the potential probiotic efficacy on growth, immunity, and gut microbiota in hybrid sturgeon (Acipenser baerii ♀ × Acipenser schrenckii ♂).","authors":"Qingfeng Su, Xiaoqian Peng, Zihui Zhang, Zhongcheng Xiong, Bowu He, Pengfei Chu, Chengke Zhu","doi":"10.1016/j.fsi.2024.110081","DOIUrl":"10.1016/j.fsi.2024.110081","url":null,"abstract":"<p><p>Probiotics are increasingly considered as an alternative to antibiotics in developing environmentally sustainable aquaculture practices. Hybrid sturgeon (Acipenser baerii ♀ × Acipenser schrenckii ♂), a globally popular species valued for its nutritional content and caviar, has limited research on host-associated probiotics. In this study, we isolated and identified Bacillus subtilis and Bacillus amyloliquefaciens from healthy hybrid sturgeon and assessed their impact on growth, immunity, gut microbiota, and transcriptome following an 8-week feeding trial. The isolated strains demonstrated strong production of protease, amylase, lipase, and cellulase, along with broad-spectrum pathogen inhibition, including Aeromonas veronii, Aeromonas sobria, and Yersinia ruckeri. Supplementation with B. subtilis and B. amyloliquefaciens significantly improved growth performance and increased survival rates against A. veronii infection. Mechanistically, probiotics altered gut microbiota composition, enhancing digestive functions. Transcriptome analysis further revealed that probiotic supplementation boosted immune response and protein digestion and absorption. These findings suggest that B. subtilis and B. amyloliquefaciens are promising probiotic candidates for the hybrid sturgeon industry, offering effective protection against A. veronii infection.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110081"},"PeriodicalIF":4.1,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immunomodulatory and antioxidative effects of curcumin-supplemented diets against the isolated Aeromonas hydrophila in Oreochromisniloticus.","authors":"Ghada A El-Gammal, Gehan I E Ali, Aly S Saif, Shaimaa Elbaz, Sabreen E Fadl, Nagwa I S Abu-Zahra","doi":"10.1016/j.fsi.2024.110077","DOIUrl":"10.1016/j.fsi.2024.110077","url":null,"abstract":"<p><p>Worldwide, Aeromonas hydrophila (A. hydrophila) has become a significant foodborne pathogen, causing gastrointestinal and extra-intestinal infections in humans as well as severe economic losses in fish farms. Thus, we conducted this research in two experiments. The experiment 1 investigated the prevalence of A. hydrophila among Nile tilapia in different farms in Kafrelsheikh province and its pathogenicity by detecting some of its virulence genes before being used in the in vivo experiment. The experiment 2 was done to investigate the effect of dietary curcumin on growth performance, humoral immunity, antioxidants, and histopathology of Nile tilapia through a sixty-day feeding trial. Nile tilapia was divided into 3 groups, each in 3 replicates. Fish were given diets that included 0.0 (C, control), 1 % (10 g curcumin/kg diet; G1), and 2 % curcumin/kg diet (20 g curcumin/kg diet; G2). Moreover, the antibacterial effect of dietary curcumin against isolated bacteria in experiment 1 was investigated through experimental infection. The study's findings of experiment 1 indicated that the incidence of isolated A. hydrophila was 28 %. Moreover, the virulence aerolysin (aerA) and haemolysin (hlyA) genes were found in 100 % and 50 % of the isolates, respectively. The experiment 2 outcomes demonstrated that the growth-modulating impacts of dietary curcumin were notably noticed in final weight, feed intake, weight gain%, and specific growth rate without pathological lesions in the different organs. In addition, the white blood cell and lymphocyte counts significantly (P < 0.05) increased in Nile tilapia fed dietary curcumin. Similar improvements in humoral immunity (lysozyme and phagocytic activity) and antioxidants (catalase and superoxide dismutase) were seen in the 1 % curcumin group (G1). The experimental infection with a virulent strain of A. hydrophila that was previously isolated in experiment 1 showed a significant decrease in the morbidity and mortality rates in the 1 % curcumin treatment group (G1), followed by the 2 % group (G2). The present study concluded that under current experimental conditions, dietary curcumin at a dose of 10 g/kg diet proves effective, but the dose of 1 % may not be optimal for tilapia.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110077"},"PeriodicalIF":4.1,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}