Probiotic and postbiotic effects of Bacillus velezensis AAHM-BV2354 on boosting immunity, growth performance, antioxidant activity and resistance to Edwardsiella tarda infection in pangasius (Pangasianodon hypophthalmus)

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-07-11 DOI:10.1016/j.fsi.2025.110561

Nugroho Wiratama , Benchawan Kumwan , Pakapon Meachasompop , Yosapon Adisornprasert , Prapansak Srisapoome , Kim D. Thompson , Phornphan Phrompanya , Patcharapong Thangsunan , Pattanapong Thangsunan , Kanokporn Saenphet , Supap Saenphet , Wararut Buncharoen , Anurak Uchuwittayakul

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引用次数: 0

Abstract

The increasing demand for Pangasianodon hypophthalmus underscores the importance of addressing disease-related productivity challenges. Probiotic feed additives present a promising approach for improving disease management, enhancing productivity, and promoting sustainable growth in pangasius farming. This study investigated the impact of dietary supplementation with probiotics, postbiotics, and their synergistic combination, utilising Bacillus velezensis AAHM-BV2354, on fish health, growth performance, oxidative stress status, innate immune responses and disease resistance in pangasius, aimed at preventing Edwardsiella tarda infection. The experimental design featured five treatment groups, each receiving feed coated with different components of Bacillus velezensis AAHM-BV2354, including cells (BV_Cell), cell-free supernatant (BV_Cfs), and a combination of both (BV_Cell + Cfs), with 2 type of control group were feed top-dressed with 100 mL of 0.85 % NaCl/kg diet (Control) and feed coated with 100 mL of TSB medium/kg diet (C_TSB) for 30 days before the challenge test with Edwardsiella tarda. The findings revealed that the BV_Cfs treatment group exhibited a notable enhancement in SGR, ADG, and upregulation gh1 gene. Bacillus velezensis AAHM-BV2354 demonstrated protective effects by reducing oxidative stress in MDA levels and boosting antioxidant enzymes in specific organs. Gene expression analysis revealed an upregulation in the expression of immune genes (lygl1, tgfb, tnf, b2ml), proinflammatory genes (litaf, ifngr1l, c3, il13, il1b), and antioxidant genes (cusr, cat, gpx3, hspa13, nos1) in various fish organs after administering probiotics, postbiotics, and their synergistic combination of Bacillus velezensis AAHM-BV2354. The subsequent challenge test with E. tarda showed that the BV_Cell treatment achieved the highest survival rate (90.62 %). These results indicate that all bioactive components of B. velezensis AAHM-BV2354 can serve as natural immunostimulants, enhancing the health and growth of pangasius.

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韦氏芽孢杆菌AAHM-BV2354对巴沙鱼免疫、生长性能、抗氧化活性和抗迟缓爱德华氏菌感染的益生菌和后生菌效应

对穿山甲低眼症的需求日益增加，强调了解决与疾病有关的生产力挑战的重要性。益生菌饲料添加剂为改善巴沙鱼养殖的疾病管理、提高生产力和促进可持续增长提供了一种有希望的方法。本研究以韦氏芽孢杆菌AAHM-BV2354为载体，研究了在饲料中添加益生菌、后益生菌及其协同组合对巴沙鱼健康、生长性能、氧化应激状态、先天免疫反应和抗病能力的影响，旨在预防迟达爱德华氏菌感染。试验设计分为5个处理组，每组分别以细胞（BV_Cell）、无细胞上清（BV_Cfs）和两者的组合（BV_Cell + Cfs）包被velezensis AAHM-BV2354不同成分的饲料，其中2个对照组分别以100 mL 0.85% NaCl/kg日粮（对照）和100 mL TSB培养基/kg日粮（C_TSB）包被饲料，饲喂30 d，然后进行迟达Edwardsiella攻毒试验。结果显示，BV_Cfs治疗组SGR、ADG显著增强，gh1基因上调。velezensis AAHM-BV2354通过降低氧化应激MDA水平和提高特定器官的抗氧化酶水平而具有保护作用。基因表达分析显示，给药益生菌、后益生菌及其协同组合velezensis AAHM-BV2354后，免疫基因（lygl1、tgfb、tnf、b2ml）、促炎基因（litaf、ifngr1l、c3、il13、il1b）和抗氧化基因（cusr、cat、gpx3、hspa13、nos1）在鱼类各器官中的表达上调。随后用e.t arda攻毒试验表明，BV_Cell处理的存活率最高（90.62%）。上述结果表明，白颡鱼AAHM-BV2354的所有活性成分均可作为天然免疫刺激剂，促进巴沙鱼的健康和生长。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.