全基因组重测序揭示了遗传选择的大口黑鲈（Micropterus salmoides）的抗病性和遗传特征

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-07-08 DOI:10.1016/j.fsi.2025.110558

Hui Sun , Jixiang Hua , Yifan Tao , Jie He , Qingchun Wang , Yalun Dong , Jixiang He , Jun Qiang

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引用次数: 0

摘要

大口黑鲈ranv （LMBV）具有高度传染性和致死率，是对养殖大口黑鲈的严重威胁。课题组以美国北部大口黑鲈亚种为基础种群，通过家族选择，结合分子标记辅助选择，对以抗LMBV为目标性状的大口黑鲈进行遗传改良。到2023年，我们已经培育出35个F2代抗lmbv家族。通过LMBV病毒攻毒实验，筛选出存活率最高的前6个品系，于2024年构建f3代抗性群体（SF）。感染LMBV病毒后，SF群体的7天存活率比未选择的对照群体（CF）高28.9%。全基因组重测序结果显示，SF群体的遗传多样性（PIC = 0.1557 vs. 0.1029, Ao = 1.8935 vs. 1.4329, Ho = 0.1647 vs. 0.1146）高于CF群体。通过选择性扫描分析（FST- zhp /FST-θπ）对SF群体和CF群体中选择的49个基因组区域进行了鉴定，并对169个候选基因进行了定位。其中免疫调控相关基因atp7b、nampt、hpcal4和ppsst1/2在SF群体中经过强化选择被鉴定为抗病关键候选基因。这些结果为研究大口黑鲈抗LMBV的遗传机制提供了新的信息，为进一步研究该病毒的抗性和抗病品种的选择提供了理论支持。所鉴定的基因和基因组区域对大口黑鲈的精准育种具有重要意义。

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Disease resistance and genetic characteristics of genetically selected largemouth bass (Micropterus salmoides) revealed by whole genome resequencing

Largemouth bass ranavirus (LMBV) is a serious threat to aquacultured largemouth bass (Micropterus salmoides) because it is highly infectious and lethal. Our research group has carried out genetic improvement of largemouth bass with LMBV resistance as a target trait through family selection, combined with molecular marker-assisted selection, using the northern U.S.A. subspecies of largemouth bass as a foundation population. By 2023, we had generated 35 F₂ generation LMBV-resistant families. The top six lines with the highest survival rates were selected through LMBV virus challenge experiments, leading to the construction of an F₃-generation resistant population (SF) in 2024. After LMBV virus infection, the 7-day survival rate of the SF population was 28.9 % higher than that of the unselected control population (CF). Whole genome resequencing revealed higher genetic diversity of the SF population (PIC = 0.1557 vs. 0.1029, Ao = 1.8935 vs. 1.4329, Ho = 0.1647 vs. 0.1146) compared with the CF population. Forty-nine genomic regions selected in the SF population vs. the CF population were identified by selective scanning analysis (F_ST-ZHp/F_ST-θπ), and 169 candidate genes were localized. Among them, the immune regulation-related genes atp7b, nampt, hpcal4, and ppsst1/2 were identified as key candidate genes for disease resistance under intensive selection in the SF population. These results provide new information about the genetic mechanism of LMBV resistance in largemouth bass, and theoretical support for further research on virus resistance and the selection of disease-resistant varieties. The identified genes and genomic regions are of great significance for the precision breeding of largemouth bass.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.