Epigallocatechin gallate (EGCG) inhibits Micropterus salmoides rhabdovirus replication via suppressing viral attachment and infectivity

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-05-29 DOI:10.1016/j.fsi.2025.110467

Yuhang Wang , Shuangshuang Mao , Xiaoran Feng , Reng Qiu , Na Li , Lunguang Yao , Yinghui Qin

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引用次数: 0

Abstract

Micropterus salmoides rhabdovirus (MSRV) remains an economically important pathogen in the global largemouth bass industry, effective drugs to control the virus are still lacking. Epigallocatechin gallate (EGCG), the most abundant and bioactive catechin in green tea, possesses multiple biological activities, including widespread antiviral effects. However, whether EGCG can inhibit MSRV infection remains unknown. Here, we tried to determine the role of EGCG in MSRV replication and to investigate its underlying mechanisms. Cell viability and drug treatment assays showed that EGCG significantly inhibited MSRV replication at the concentration range of 1–40 μM without obvious cytotoxicity. Pretreatment assay indicated that treating EPC cells with EGCG before MSRV infection has no significant inhibition on MSRV replication, suggesting the antiviral action of EGCG might not target to MSRV receptor and/or related proteins required for MSRV infection. Time of addition assays indicated that EGCG exerted antiviral activity in the early stage of MSRV life cycle. Further studies showed that EGCG possesses excellent inhibitory effects on the attachment step of MSRV. The virucidal assay revealed EGCG directly attenuated MSRV infectivity, which may partly account for its anti-MSRV effect. In vivo experiments showed that EGCG improved the survival rate of MSRV-challenged fish by 21 %. Altogether, EGCG exhibited a strong anti-MSRV activity and might be a promising therapeutic drug for MSRV infection.

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表没食子儿茶素没食子酸酯（EGCG）通过抑制病毒的附着和传染性来抑制小鱼横纹肌病毒的复制

在全球大口黑鲈产业中，横纹肌小翼病毒（Micropterus salmoides rhabdovirus， MSRV）仍是一种重要的经济病原菌，目前还缺乏有效的防治药物。表没食子儿茶素没食子酸酯（EGCG）是绿茶中含量最多、最具生物活性的儿茶素，具有多种生物活性，包括广泛的抗病毒作用。然而，EGCG是否能抑制MSRV感染尚不清楚。在这里，我们试图确定EGCG在MSRV复制中的作用，并研究其潜在机制。细胞活力和药物处理实验表明，EGCG在1 ~ 40 μM浓度范围内显著抑制MSRV复制，无明显细胞毒性。预处理实验表明，在MSRV感染前用EGCG处理EPC细胞对MSRV复制没有明显的抑制作用，提示EGCG的抗病毒作用可能不是针对MSRV感染所需的MSRV受体和/或相关蛋白。添加时间分析表明，EGCG在MSRV生命周期早期发挥抗病毒活性。进一步研究表明，EGCG对MSRV的附着步骤具有良好的抑制作用。毒力实验显示EGCG直接减弱了MSRV的传染性，这可能是其抗MSRV作用的部分原因。体内实验表明，EGCG可使msrv侵染鱼的存活率提高21%。综上所述，EGCG具有较强的抗MSRV活性，可能是一种有前景的治疗MSRV感染的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.