Inhibition of opioid growth factor receptor (OGFR) promotes wound healing in the freshwater pearl mussel (Hyriopsis schlegelii)

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-06-27 DOI:10.1016/j.fsi.2025.110530

Kaixin Chen , Kou Peng , Hao Zhang , Jiaqian Li , Xinyue Zheng , Chunfu Chen , Xiaoying Zeng

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引用次数: 0

Abstract

Hyriopsis schlegelii, widely regarded as the most economically significant freshwater pearl mussel farmed in China, is known for producing high-quality pearls. The damage inflicted on the organism by pearl insertion is incalculable. Opioid growth factor receptor (OGFR), as a receptor for the opioid growth factor (OGF; [Met⁵]-enkephalin), plays essential roles in regulating cell proliferation and wound healing. Nonetheless, the role of OGFR in H. schlegelii wound healing remains poorly understood. In this paper, a cDNA of 3662 bp for the pearl mussel H.schlegelii OGFR (hereinafter referred to as HsOGFR) was obtained using the rapid amplification of cDNA ends (RACE) method. The complete cDNA sequence of HsOGFR contains a 2640 bp open reading frame (ORF) encoding 879 amino acids. The amino acid sequences of HsOGFR are 43–63 % similar to those of other OGFRs. The N-terminal of HsOGFR contains a conserved OGFR_N domain. Transcripts of HsOGFR were expressed in all tested tissues, with the highest expression observed in the hepatopancreas. Histological evaluation demonstrated that RNA interference-mediated HsOGFR knockdown (via dsRNAs) or pharmacological inhibition with naloxone significantly accelerated wound healing following the removal of mantle tissues in the mussels. Further qRT-PCR assays revealed that the mRNA expression of HsOGFR was down-regulated in mantle and haemocytes following wound treatment (P < 0.05). Concomitantly, HsCDKN1 and HsCDKN3 expression was moderately downregulated at the incision site post-wounding (P < 0.05). Following HsOGFR interference, the expression levels of HsKi67, HsCCND, HsCDKL1 and HsCDK6 were significantly upregulated (P < 0.05) compared with the wound group. Conversely, HsCDKN1 and HsCDKN3 expression was further downregulated after HsOGFR blockade (P < 0.05). Collectively, these findings demonstrate that HsOGFR, as a homolog of OGFRs, is significantly involved in tissue repair in mollusks, and might work through cell cycle related proteins above.

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抑制阿片生长因子受体（OGFR）促进淡水珍珠贻贝伤口愈合

schlegelhyriopsis schlegelii被广泛认为是中国养殖的最具经济意义的淡水珍珠贻贝，以生产高质量的珍珠而闻名。珍珠植入对生物体造成的损害是无法估量的。阿片生长因子受体（OGFR），作为阿片生长因子（OGF）受体；[Met5]-脑啡肽)，在调节细胞增殖和伤口愈合中起重要作用。尽管如此，OGFR在施莱格氏杆菌伤口愈合中的作用仍然知之甚少。本文采用cDNA末端快速扩增（RACE）方法，获得了珍珠贻贝H.schlegelii OGFR（以下简称HsOGFR）全长3662 bp的cDNA。HsOGFR全长cDNA序列包含2640bp的开放阅读框（ORF），编码879个氨基酸。HsOGFR的氨基酸序列与其他ogfr相似度为43 ~ 63%。HsOGFR的n端包含一个保守的OGFR_N结构域。HsOGFR转录本在所有检测组织中均有表达，肝胰腺中表达量最高。组织学评估表明，RNA干扰介导的HsOGFR敲低（通过dsRNAs）或纳洛酮的药理学抑制显著加速了贻贝去除套膜组织后的伤口愈合。进一步的qRT-PCR分析显示，创伤处理后，套膜和血细胞中HsOGFR mRNA表达下调(P <；0.05)。与此同时，HsCDKN1和HsCDKN3的表达在伤后切口部位中度下调(P <；0.05)。HsOGFR干扰后，hsk67、HsCCND、HsCDKL1和HsCDK6的表达水平显著上调(P <；0.05)，与创面组比较。相反，阻断HsOGFR后，HsCDKN1和HsCDKN3的表达进一步下调(P <；0.05)。总之，这些发现表明，HsOGFR作为ogfr的同源物，显著参与了软体动物的组织修复，并可能通过上述细胞周期相关蛋白起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.