An in vitro study of type I interferon and inflammatory markers induced by dsRNA and dsRNA-phytoglycogen in rainbow trout

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-06-28 DOI:10.1016/j.fsi.2025.110524

Kristof Jenik , Tania Rodríguez-Ramos , Adebisi Sanyaolu , Brian Dixon , Stephanie DeWitte-Orr

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引用次数: 0

Abstract

Understanding the induction patterns for rainbow trout type I interferon and inflammatory markers is essential for the development of new antipathogenic therapeutics and vaccines as well as enhancing aquaculture productivity and biosecurity. Type I interferons (ex. ifn1) and interferon stimulated genes (ex. vig-3) as well as inflammatory markers such as interferon gamma (ifn-γ) and interleukin 1beta (il-1β) all play a crucial role in protecting rainbow trout against pathogen infection. One strategy to better understand how fish defend themselves is by studying the effects of synthetic viral dsRNA analogues such as polyinosinic:polycytidylic acid (poly IC) on the fish innate immune response. The current work utilizes a phytoglycogen-based nanoparticle (Nanodendrix; NDx) to enhance the immunostimulatory effects of poly IC in three rainbow trout cell lines derived from monocyte/macrophages (RTS11), gonads (RTG-2) and gill (RTgill-W1). Variations in innate immune responses were observed between cell lines, between poly IC and poly IC + NDx treatment groups and between transcript, protein and antiviral response levels of study. The poly IC + NDx complex demonstrated prolonged immune stimulation up to 96h post-treatment and exhibited significant inhibition of infectious pancreatic necrosis virus (IPNV) replication. These findings highlight the potential of poly IC + NDx complexes as a novel antiviral therapeutic approach for future in vitro and in vivo studies.

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虹鳟鱼I型干扰素及dsRNA-植物糖原诱导炎症标志物的体外研究。

了解虹鳟鱼I型干扰素和炎症标志物的诱导模式对于开发新的抗病原疗法和疫苗以及提高水产养殖生产力和生物安全至关重要。I型干扰素（如ifn1）和干扰素刺激基因（如vig3）以及炎症标志物(如干扰素γ （ifn-γ)和白细胞介素1β (il-1β)）都在保护虹鳟免受病原体感染方面发挥着至关重要的作用。更好地了解鱼类如何保护自己的一个策略是研究合成病毒dsRNA类似物，如多肌苷：多胞酸（poly IC）对鱼类先天免疫反应的影响。目前的研究利用了一种基于植物糖原的纳米粒子(Nanodendrix；NDx)增强poly IC对来自单核/巨噬细胞（RTS11）、性腺（RTG-2）和鳃（RTgill-W1）的三种虹鳟鱼细胞系的免疫刺激作用。观察了不同细胞系之间、多IC和多IC+NDx治疗组之间以及转录物、蛋白和抗病毒反应水平之间的先天免疫反应的差异。poly IC+NDx复合物在治疗后可延长至96小时的免疫刺激，并表现出对感染性胰腺坏死病毒（IPNV）复制的显著抑制。这些发现强调了poly IC+NDx复合物作为一种新的抗病毒治疗方法在未来的体外和体内研究中的潜力。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.