Experimental and clinical endocrinology最新文献_第6页

Bone mineral density and calcium regulating hormones in patients with inflammatory bowel disease (Crohn's disease and ulcerative colitis). 炎症性肠病(克罗恩病和溃疡性结肠炎)患者的骨密度和钙调节激素

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211264

S H Scharla, H W Minne, U G Lempert, G Leidig, M Hauber, R Raedsch, R Ziegler

{"title":"Bone mineral density and calcium regulating hormones in patients with inflammatory bowel disease (Crohn's disease and ulcerative colitis).","authors":"S H Scharla, H W Minne, U G Lempert, G Leidig, M Hauber, R Raedsch, R Ziegler","doi":"10.1055/s-0029-1211264","DOIUrl":"https://doi.org/10.1055/s-0029-1211264","url":null,"abstract":"Inflammatory bowel disease (Crohn's disease and ulcerative colitis) is associated with decreased bone mineral density and increased risk of osteoporosis. However, the pathogenesis of this bone loss is not yet fully understood. In the present study we measured lumbar bone mineral density (by dual photon absorptiometry), serum levels of parathyroid hormone (PTH) and vitamin D metabolites, and serum markers of bone turnover (alkaline phosphatase and osteocalcin) in 15 patients with Crohn's disease and in 4 patients with ulcerative colitis. The median duration of the disease was 4 years and the median lifetime steroid dose was 10g of prednisone. We compared our results to a control group of 19 normal persons, who were matched for age and sex to the patients. We found that lumbar bone density was reduced by 11% in patients compared with control persons (Z-score -0.6 +/- 0.6 versus -0.1 +/- 0.8; p < 0.05). In patients, the serum levels of PTH, 25-hydroxyvitamin D3, and calcitriol (1,25(OH)2D3) were significantly reduced compared with control persons. Serum alkaline phosphatase activity (AP) was significantly higher in the patients and was inversely related to lumbar bone density. Osteocalcin values were not different between patients and control persons. There was also no difference in serum levels of calcium between the two groups, whereas phosphorus levels were higher in patients. We conclude that malabsorption of calcium was not a primary cause of bone loss in our patients, because we did not find secondary hyperparathyroidism. Accordingly, we did not find a severe vitamin D deficiency, since 25-hydroxyvitamin D3 levels were within the normal range.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"102 1","pages":"44-9"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19000227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Effects of music treatment on salivary cortisol in patients exposed to pre-surgical stress. 音乐治疗对术前应激患者唾液皮质醇的影响。

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211273

B Miluk-Kolasa, Z Obminski, R Stupnicki, L Golec

引用次数: 110

Intracellular sex hormone-binding globulin (SHBG) in normal and neoplastic breast tissue--an additional marker for hormone dependency? 正常和肿瘤乳腺组织中细胞内性激素结合球蛋白(SHBG)——激素依赖的额外标志?

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211300

S Meyer, C Brumm, H E Stegner, G H Sinnecker

{"title":"Intracellular sex hormone-binding globulin (SHBG) in normal and neoplastic breast tissue--an additional marker for hormone dependency?","authors":"S Meyer, C Brumm, H E Stegner, G H Sinnecker","doi":"10.1055/s-0029-1211300","DOIUrl":"https://doi.org/10.1055/s-0029-1211300","url":null,"abstract":"Recent studies indicate that in addition to free diffusion, uptake of sex hormones into target cells is mediated by sex hormone-binding globulin (SHBG). The purpose of this study was to investigate localization and distribution of SHBG in normal and neoplastic breast tissue. We examined 31 normal, 21 non-invasive, 52 invasive breast cancer tissues and 33 cases of recurrences and metastases of breast cancer immunohistochemically for SHBG by the ABC-peroxidase method, using a polyclonal, monospecific antiserum derived from rabbit. The proportion of stained cells was evaluated semiquantitatively. In 81 malignant cases the oestrogen receptor (ER) content was evaluated by the ER-ICA method. Positive staining for SHBG was found exclusively in epithelial cell cytoplasm. Benign tissue was focally SHBG-positive and showed more stained cells in proliferating epithelium. Staining of neoplastic tissue was more heterogeneous. Half of the non-invasive carcinomas were SHBG-positive; particularly the highly differentiated. Independent of subtype and differentiation, invasive tumours were SHBG-negative in 32.5% of cases, while 19.3% were SHBG-positive in most cells. In 13 cases of invasive carcinomas, associated intraductal parts showed more staining for SHBG than the invasive tissue. Recurrences and metastases of breast cancer were SHBG-negative in 45.5% of cases, while only 3% were positive in most cells. SHBG-staining was unrelated to ER content. These results suggest that the demonstration of cytoplasmic SHBG represents a physiological feature of breast epithelium and its presence is compatible with a mechanism for cellular uptake of SHBG-bound sex hormones preceding their interaction with nuclear receptors.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"102 4","pages":"334-40"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211300","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18535776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Endocrine characterization of the new dopamine autoreceptor agonist roxindole. 新型多巴胺自身受体激动剂罗新多的内分泌特性。

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211293

K Wiedemann, M Kellner

{"title":"Endocrine characterization of the new dopamine autoreceptor agonist roxindole.","authors":"K Wiedemann, M Kellner","doi":"10.1055/s-0029-1211293","DOIUrl":"https://doi.org/10.1055/s-0029-1211293","url":null,"abstract":"Roxindole (5-Hydroxy-3-(4-phenyl-1,2,3,6-tetrahydropyridil-(1)-butyl)-indol) is a newly developed compound with a high dopamine autoreceptor agonistic potency at D2 receptors. Evaluation of roxindole for clinical purposes in psychiatric patients revealed that the substance has contrary to expectation merely negligible antipsychotic but considerable antidepressive effects. Interestingly it is nearly devoid of any side effects. To further characterize the endocrine effects, 1 mg roxindole was applied at 09:00 to 6 male volunteers and the effects compared to placebo. From 08:00 to 13:00 blood samples were drawn every 30 min. No effect on adrenocorticotropin and cortisol secretion was observed, neither mean plasma concentrations nor area values differed significantly. 90 min after roxindole administration an enhanced release of growth hormone could be observed, comparison of mean hormonal concentration revealed statistical significance (p < 0.05). Also the area values differed significantly (roxindole vs. placebo mean +/- SD 18.1 +/- 17.1 vs. 7.7 +/- 8.0 micrograms x h/l; p < 0.05). After roxindole also a pronounced reduction of prolactin plasma levels from 5.3 +/- 1.4 micrograms/l to 1.1 +/- 0.4 microgram/l within 150 min could be observed. Mean plasma concentrations (p < 0.05) and area values differed significantly (roxindole vs. placebo mean +/- SD 14.8 +/- 13.5 vs. 34.4 +/- 18.1; p < 0.05). Roxindole had no effect on blood pressure parameters and heart rate. The major findings of the present exploratory study are, that roxindole has potent effects on the facilitation of growth hormone secretion and the inhibition of prolactin release. Both findings are complementary and point also to interesting postsynaptic dopamine receptor agonistic effects.","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"102 4","pages":"284-8"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211293","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18812573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effect of morphine on hypothalamic catecholamine and serotonin level in relation to the stress-induced pituitary-adrenocortical activation in the rat. 吗啡对应激诱导大鼠下丘脑儿茶酚胺和血清素水平的影响。

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211296

L Debreceni, G Hartmann, B Debreceni

{"title":"Effect of morphine on hypothalamic catecholamine and serotonin level in relation to the stress-induced pituitary-adrenocortical activation in the rat.","authors":"L Debreceni, G Hartmann, B Debreceni","doi":"10.1055/s-0029-1211296","DOIUrl":"https://doi.org/10.1055/s-0029-1211296","url":null,"abstract":"The relationship between the hypothalamic catecholamine and serotonin level as well as the activation of the pituitary-adrenal axis was investigated after administration or morphine (MO) in the rat. Five mg/kg b. wt. of MO induced a significant increase in norepinephrine and a 78%, but insignificant, increase in dopamine level of the hypothalamus within 60 min without changing corticosterone secretion. Electric footshock, in addition to elevating hypothalamic norepinephrine and dopamine levels, significantly increased the pituitary-adrenocortical response in the MO pretreated rats. Five mg/kg b. wt. of MO, or electric footshock alone did not influence the hypothalamic serotonin level within 60 min, but the hypothalamic serotonin level decreased significantly in the MO pretreated, electrically shocked animals. We conclude, that 1) low dose of MO may induce changes of the hypothalamic catecholamine levels without influencing pituitary-adrenocortical activation. 2) enhanced hypothalamic catecholamines by MO did not prevent increasing pituitary-adrenocortical response elicited by stress. It appears, that the hypothalamic catecholaminergic mechanism which may inhibit ACTH release during stimulation does not function in the MO treated rats.","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"102 4","pages":"307-12"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211296","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18812576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Luteal versus placental progesterone: the situation in the cow, pig and bitch. 黄体与胎盘黄体酮:奶牛、猪和母狗的情况。

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211281

H H Meyer

引用次数: 23

Gestagens in peripheral, uterine, ovarian and fetal blood in cows near term. 近足月奶牛外周血、子宫、卵巢和胎儿血液中的孕激素。

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211282

T Janowski

引用次数: 3

Paracrine and autocrine functions of the placenta: a key to the success of viviparity? 胎盘的旁分泌和自分泌功能:胎生成功的关键?

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211290

R B Heap

{"title":"Paracrine and autocrine functions of the placenta: a key to the success of viviparity?","authors":"R B Heap","doi":"10.1055/s-0029-1211290","DOIUrl":"https://doi.org/10.1055/s-0029-1211290","url":null,"abstract":"The evolution of specific nuclear transcriptional regulators has endowed tissues of the reproductive system with responsiveness to small hydrophobic compounds such as steroids. Steroids are widely distributed in Nature and their distribution in prokaryotes and eukaryotes has given rise to the concept that their hormonal role came about by target organ specialization and not by the evolution of steroids themselves. Specific nuclear receptors for progesterone in the uterus are prominent during the establishment and maintenance of pregnancy. Anti-progesterone antagonists which interfere with receptor-mediated DNA activation abrogate pregnancy and thus emphasize the functional importance of the pathways by which the effects of progesterone as an extracellular signal are transduced. Comparative studies show that progesterone itself can be ovarian or placental in origin. This seems to reflect the evolution of different mechanisms of endocrine function rather than any obvious selective advantage being associated with the source of hormone secretion. For this reason, the question of whether the endocrine function of the placenta is obligatory for the adoption of viviparity in mammals is far from certain, and should be considered as an evolutionary option rather than a sine qua non. Of growing importance is the idea that the interaction between trophoblast and endometrial cells controls the degree of invasiveness at implantation and immunoreactivity.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"102 3","pages":"262-8"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211290","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18989136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Placental structure: in a comparative aspect. 胎盘结构:在比较方面。

Experimental and clinical endocrinology Pub Date : 1994-01-01 DOI: 10.1055/s-0029-1211275

R Leiser, P Kaufmann

引用次数: 391

Neurosteroids do not regulate proopiomelanocortin-gene expression in pituitary cells. 神经类固醇不调节垂体细胞中促黑素原基因的表达。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211224

H Vedder, F Parlow, W Zieglgänsberger, F Holsboer

{"title":"Neurosteroids do not regulate proopiomelanocortin-gene expression in pituitary cells.","authors":"H Vedder, F Parlow, W Zieglgänsberger, F Holsboer","doi":"10.1055/s-0029-1211224","DOIUrl":"https://doi.org/10.1055/s-0029-1211224","url":null,"abstract":"Brain-derived steroids (neurosteroids) constitute a new group of steroids synthesized in brain cells independently from peripheral sources. While the membraneous actions of these steroids have been characterized in some detail, their genomic effects are less well studied up to now. In the present study, the action of the neurosteroids dehydroepiandrosterone, pregnenolone and pregnanolone on proopiomelanocortin (POMC)-gene expression was investigated in pituitary AtT 20 cells, which were cultured under serum-free conditions and at strictly controlled steroid levels. In these cells, POMC-gene expression is known to be negatively regulated by adrenocortical glucocorticoids. Quantitative Northern blot analysis showed that none of these neurosteroids or their conjugates affected POMC-mRNA levels even at the highest concentrations employed. The 21-OH steroid corticosterone was used as a control and markedly suppressed the synthesis of POMC-mRNA. These results suggest that neurosteroids do not control POMC-gene expression at the level of the pituitary corticotroph and exclude significant effects of these compounds at the glucocorticoid receptor.","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"101 3","pages":"161-5"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19213038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5