正常和肿瘤乳腺组织中细胞内性激素结合球蛋白(SHBG)——激素依赖的额外标志?

S Meyer, C Brumm, H E Stegner, G H Sinnecker
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引用次数: 21

摘要

最近的研究表明,除了自由扩散外,性激素进入靶细胞的摄取是由性激素结合球蛋白(SHBG)介导的。本研究的目的是探讨SHBG在正常和肿瘤乳腺组织中的定位和分布。采用兔源性多克隆单特异性抗血清,采用abc -过氧化物酶法对31例正常、21例非侵袭性、52例侵袭性乳腺癌组织和33例复发及转移性乳腺癌组织进行SHBG免疫组化检测。半定量评价染色细胞比例。用ER- ica法测定81例恶性肿瘤的雌激素受体(ER)含量。SHBG阳性染色仅在上皮细胞细胞质中发现。良性组织局部shbg阳性,增殖上皮细胞染色较多。肿瘤组织的染色更不均匀。半数非侵袭性癌为shbg阳性;特别是高度分化的。侵袭性肿瘤与亚型和分化无关,32.5%的病例为shbg阴性,19.3%的细胞为shbg阳性。在13例浸润性癌中,相关导管内部位的SHBG染色高于浸润性组织。45.5%的乳腺癌复发和转移病例为shbg阴性,而大多数细胞中只有3%为阳性。shbg染色与ER含量无关。这些结果表明,细胞质SHBG的存在代表了乳腺上皮的一种生理特征,它的存在与SHBG结合的性激素在与核受体相互作用之前的细胞摄取机制是相容的。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intracellular sex hormone-binding globulin (SHBG) in normal and neoplastic breast tissue--an additional marker for hormone dependency?

Recent studies indicate that in addition to free diffusion, uptake of sex hormones into target cells is mediated by sex hormone-binding globulin (SHBG). The purpose of this study was to investigate localization and distribution of SHBG in normal and neoplastic breast tissue. We examined 31 normal, 21 non-invasive, 52 invasive breast cancer tissues and 33 cases of recurrences and metastases of breast cancer immunohistochemically for SHBG by the ABC-peroxidase method, using a polyclonal, monospecific antiserum derived from rabbit. The proportion of stained cells was evaluated semiquantitatively. In 81 malignant cases the oestrogen receptor (ER) content was evaluated by the ER-ICA method. Positive staining for SHBG was found exclusively in epithelial cell cytoplasm. Benign tissue was focally SHBG-positive and showed more stained cells in proliferating epithelium. Staining of neoplastic tissue was more heterogeneous. Half of the non-invasive carcinomas were SHBG-positive; particularly the highly differentiated. Independent of subtype and differentiation, invasive tumours were SHBG-negative in 32.5% of cases, while 19.3% were SHBG-positive in most cells. In 13 cases of invasive carcinomas, associated intraductal parts showed more staining for SHBG than the invasive tissue. Recurrences and metastases of breast cancer were SHBG-negative in 45.5% of cases, while only 3% were positive in most cells. SHBG-staining was unrelated to ER content. These results suggest that the demonstration of cytoplasmic SHBG represents a physiological feature of breast epithelium and its presence is compatible with a mechanism for cellular uptake of SHBG-bound sex hormones preceding their interaction with nuclear receptors.(ABSTRACT TRUNCATED AT 250 WORDS)

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