神经类固醇不调节垂体细胞中促黑素原基因的表达。

H Vedder, F Parlow, W Zieglgänsberger, F Holsboer
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引用次数: 5

摘要

脑源性类固醇(神经类固醇)是一种独立于外周来源在脑细胞中合成的新类固醇。虽然这些类固醇的膜作用已经有了一些详细的特征,但它们的基因组效应目前还没有得到很好的研究。本研究在无血清条件下严格控制激素水平培养的垂体AtT 20细胞中,研究了神经甾体脱氢表雄酮、孕烯醇酮和孕烯醇酮对POMC基因表达的影响。在这些细胞中,已知pomc基因表达受肾上腺皮质糖皮质激素的负调控。定量Northern blot分析显示,即使在最高浓度下,这些神经类固醇或其偶联物也不会影响POMC-mRNA水平。21-OH类固醇皮质酮作为对照,可明显抑制POMC-mRNA的合成。这些结果表明,神经类固醇不能控制垂体皮质激素水平的pomc基因表达,并且排除了这些化合物对糖皮质激素受体的显著影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Neurosteroids do not regulate proopiomelanocortin-gene expression in pituitary cells.

Brain-derived steroids (neurosteroids) constitute a new group of steroids synthesized in brain cells independently from peripheral sources. While the membraneous actions of these steroids have been characterized in some detail, their genomic effects are less well studied up to now. In the present study, the action of the neurosteroids dehydroepiandrosterone, pregnenolone and pregnanolone on proopiomelanocortin (POMC)-gene expression was investigated in pituitary AtT 20 cells, which were cultured under serum-free conditions and at strictly controlled steroid levels. In these cells, POMC-gene expression is known to be negatively regulated by adrenocortical glucocorticoids. Quantitative Northern blot analysis showed that none of these neurosteroids or their conjugates affected POMC-mRNA levels even at the highest concentrations employed. The 21-OH steroid corticosterone was used as a control and markedly suppressed the synthesis of POMC-mRNA. These results suggest that neurosteroids do not control POMC-gene expression at the level of the pituitary corticotroph and exclude significant effects of these compounds at the glucocorticoid receptor.

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