Environmental Technology最新文献

{"title":"Biohydrogen production from lactate derived via co-fermentation of cassava starch wastewater and glycerol in a continuous multiple tube reactor.","authors":"Rafaela Adam Baioco, Cristiane Lurdes Andreani, Jean Michel Chaves Orben, Luana Cristina Calliari Leite Rossi, Eliandra Rodio, Karina Rafagnin, Victor Vaz, Simone Damasceno Gomes","doi":"10.1080/09593330.2026.2645961","DOIUrl":"https://doi.org/10.1080/09593330.2026.2645961","url":null,"abstract":"<p><p>This study evaluated biohydrogen production in a continuous multiple tube reactor (CMTR) using a lactic acid-rich substrate derived from the co-fermentation of cassava starch wastewater (CSW) with glycerol. The process had two stages: (i) lactic acid (LA) production in an anaerobic sequential batch reactor (ASBR); and (ii) use of the LA-rich substrate in the CMTR at different organic loading rates (OLRs): 48, 72, and 96 g COD L⁻¹ d⁻¹, with a fixed hydraulic retention time of 4 h. The lactic fermentation produced a homogeneous substrate with 41% LA and 52% glycerol, suitable for hydrogen generation. CMTR performance varied with OLR: the highest OLR (96 g COD L⁻¹ d⁻¹) resulted in the greatest volumetric hydrogen production rate (1,960.3 mL H₂ L⁻¹ d⁻¹), biogas flow (9,360.9 mL d⁻¹), and COD removal (41.8%). The intermediate OLR (72 g COD L⁻¹ d⁻¹) achieved the highest hydrogen yield (8.4 mmol H₂ g⁻¹ COD), along with 95% lactic acid and 65% glycerol conversion. Metabolite profiling reinforced LA's role as a strategic substrate in promoting efficient fermentative routes, indicating a selective shift toward the butyric pathway, where lactic and acetic acids are converted into butyric acid and hydrogen. Overall, the results demonstrate that lactic pre-fermentation of CSW and glycerol produces a viable substrate for biohydrogen production, enabling the application of elevated OLRs and maintaining a pH favourable to hydrogenogenic microbial activity. The CMTR proved to be a promising system for agro-industrial waste valorisation through sustainable hydrogen generation.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermophilic biodegradation of phenol-catechol wastewater by a microbial consortium: kinetic modeling and sequencing batch reactor design.","authors":"Debapriya Maity, Pradyut Kundu, Sunita Adhikari Nee Pramanik","doi":"10.1080/09593330.2026.2664861","DOIUrl":"https://doi.org/10.1080/09593330.2026.2664861","url":null,"abstract":"<p><p>Aerobic biodegradation of mixed phenolic wastewater containing phenol and catechol was investigated under thermophilic conditions to integrate biodegradation kinetics with practical sequencing batch reactor (SBR) design. A thermotolerant microbial consortium was used to evaluate treatment performance, parameter optimization, substrate inhibition kinetics, and reactor-scale translation. Six physicochemical parameters - temperature, pH, incubation time, medium volume, inoculum size, and initial substrate concentration - were optimized to maximize removal efficiency. Under optimal conditions (45 °C, pH 8.5, 48 h, 600 mL working volume, 12% inoculum, and 1000 mg L⁻¹ total substrate), 99.9% degradation of the phenol-catechol mixture was achieved within 48 h.The biodegradation kinetics followed the Haldane substrate inhibition model, yielding kinetic parameters of μ_m = 0.061 h⁻¹, K_s = 38.65 mg L^-1, K_i = 138.24 mg L^-1, and Y_x/s = 0.0035 OD₆₀₀·L·mg^-1, with strong model agreement (R² > 0.99). Using these parameters, a pilot-scale SBR (2.2 m³ working volume) was designed with a 48 h reaction cycle, aeration rate of 1 vvm, and power input of 0.05 W L^-1, achieving 95-98% substrate removal per cycle under thermophilic and alkaline conditions.Overall, this study demonstrates the direct integration of mixed-substrate biodegradation kinetics with reactor-scale process design, bridging laboratory-scale modelling and applied wastewater treatment engineering for high-strength phenolic effluents.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-17"},"PeriodicalIF":2.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dam sediment as a soil amendment enhances plant antioxidant responses and suppresses bacterial wilt.","authors":"Tsukasa Ito, Junpei Ono, Yuta Oike, Ghazaleh Eslamloo","doi":"10.1080/09593330.2026.2665435","DOIUrl":"https://doi.org/10.1080/09593330.2026.2665435","url":null,"abstract":"<p><p>Dam sediment accumulates in reservoirs worldwide, yet its fine fractions are rarely reused. To explore the functional benefits of sediment application in agriculture, we evaluated its effects on soil properties, plant physiological responses, and bacterial wilt suppression in Micro-Tom (<i>Solanum lycopersicum</i> 'Micro-Tom'). Potting soil was amended with dam sediment at 0%, 4%, or 8% (wet basis), and plants were inoculated with <i>Ralstonia pseudosolanacearum</i>. The 8% sediment treatment markedly reduced both the progression and final severity of bacterial wilt; no plants exceeded the disease threshold, whereas all plants in the control treatment became diseased. At day 21, disease incidence (DS ≥ 3) was 100% (4/4) at 0% sediment and 0% (0/4) at 8% sediment (<i>p</i> = 0.0286). Sediment amendment was associated with changes in soil nutrient status and increased carotenoid accumulation in tomato leaves, suggesting a potential enhancement of antioxidant capacity. Peroxidase activity also increased with sediment addition, indicating potential enhancement of constitutive defense metabolism. Phenylalanine ammonia-lyase activity showed weaker differences at the late sampling time, consistent with its known role as an early-response enzyme. Hydrogen peroxide levels remained stable across treatments, suggesting that sediment-mediated resistance does not involve excessive oxidative stress. These results demonstrate that dam sediment can enhance both basal and inducible components of plant defense, contributing to reduced wilt severity. The findings highlight the potential of dam sediment as a biostimulant that promotes plant resilience while supporting sustainable resource recycling from watershed management to agriculture.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of ARGs during the maturation phase of oxytetracycline fermentation residue composting using lanthanum-modified zeolite.","authors":"Lejie Wang, Yangfan Du, Gang Wang, Ziyu Mei, Huijian Wang, Xing Li, Tingting Yu, Huiling Liu, Cuishuang Jiang, Picheng Gong","doi":"10.1080/09593330.2026.2664860","DOIUrl":"https://doi.org/10.1080/09593330.2026.2664860","url":null,"abstract":"<p><p>During the maturation phase of oxytetracycline fermentation residue (OFR) composting, persistently elevated levels of undegraded oxytetracycline (OTC) can lead to a resurgence of antibiotic resistance genes (ARGs). This study evaluates the feasibility and mechanisms of ARG regulation using natural zeolite (Z) and lanthanum-modified zeolite (La-Z) under conditions of ultra-high OTC stress. The results indicate that ARGs and mobile genetic elements (MGEs) were enriched during the cooling phase but decreased during maturation, with <i>tetM</i>, <i>tetG</i>, <i>tetX</i>, <i>intI1</i>, <i>TN916</i>, and <i>intI2</i> being the predominant elements. Z induced only a transient enrichment of ARGs, while La-Z maintained higher levels of ARGs and significantly increased MGEs. Multidimensional analyses revealed that the dynamics of ARGs were primarily influenced by synergistic interactions among physicochemical factors, MGEs, and host communities, with the combined effects surpassing individual contributions. Despite La-Z's strong ability to adsorb OTC, it unexpectedly exacerbated ARG enrichment in OFR composting, primarily due to altered physicochemical conditions, prolonged antibiotic stress, and the activation of MGEs. These findings provide a feasibility assessment and a mechanistic analysis of how Z and La-Z regulate ARGs during the maturation phase of OFR composting under ultra-high OTC levels. This study highlights the dual effects of additives in this extreme exposure context and emphasises the necessity for careful evaluation of control strategies for the safe reuse of OFR.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment performance and microbial succession of salinity-acclimated activated sludge treating saline domestic wastewater.","authors":"Hien-Thi Nguyen, Nghia Tuan Le","doi":"10.1080/09593330.2026.2664862","DOIUrl":"https://doi.org/10.1080/09593330.2026.2664862","url":null,"abstract":"<p><p>Recently, domestic wastewater has shown increasing salinization, especially in coastal and estuarine areas, posing challenges for biological treatment due to inhibited microbial activity. Among potential solutions, acclimation of activated sludge to saline conditions is considered a practical approach to enhance treatment performance. This study investigated the treatment efficiency and microbial community dynamics of salinity-acclimated activated sludge treating real domestic wastewater. Two experimental phases were conducted: (i) assessing traditional sludge under increasing salinity, and (ii) evaluating acclimated sludge at higher salinity (1.25%). Using real wastewater in 5-hour batch reactors, results showed that traditional sludge was ineffective at 1.25% salinity. After 21 days of acclimation (0.25% and 0.5%), COD and NH₄⁺ removal efficiencies improved to 11-13% and 22-25%, respectively, under 1.25% salinity. Microbial analysis revealed a decline in bacterial richness with increasing salinity. Concurrently, halotolerant and halophilic microorganisms were selectively enriched. These findings support the application of salinity-acclimated sludge as seed biomass for saline domestic wastewater treatment in WWTPs.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-assisted deep eutectic solvent leaching for efficient and selective extraction of rare earth elements from coal fly ash.","authors":"Changquan Men, Shenjun Qin, Jiajia Hou, Daoyuan Zhao, Shaozhuo Zhang, Hemin Gao, Chenxiao Guo, Xiaolong Yin, Shenyong Li","doi":"10.1080/09593330.2026.2668855","DOIUrl":"https://doi.org/10.1080/09593330.2026.2668855","url":null,"abstract":"<p><p>Rare earth elements are strategic and critical materials for addressing the global energy crisis and advancing sustainable development goals. Coal fly ash, a major byproduct of coal-fired power plants, has emerged as a promising secondary source of REEs. However, conventional extraction methods suffer from low efficiency, high acid consumption, and significant environmental concerns. This study developed a microwave-assisted deep eutectic solvents leaching process for efficient and selective REE recovery from coal fly ash. Three DESs were synthesized using choline chloride as the hydrogen bond acceptor with p-toluenesulfonic acid, lactic acid, or formic acid as hydrogen bond donors. Among these, the ChCl-PTSA DES exhibited superior leaching performance due to its stronger acidity and higher ionization degree. Under optimized conditions, a REEs leaching efficiency of 89.16% was achieved. FT-IR and ¹H NMR analyses confirmed that DES formation is driven by hydrogen-bonding interactions, while the leaching mechanism involves synergistic protonation of the aluminosilicate matrix and coordination of liberated REE³⁺ ions with DES functional groups. This work establishes microwave-assisted DES leaching as a highly efficient, rapid, and sustainable strategy for REEs recovery from coal fly ash, offering a promising alternative to conventional hydrometallurgical processes.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of microbial interactions in hybrid biofilm system with low organic loading to micropollutant removal.","authors":"Gulsum Emel Zengin, Roya Dadkhah, Dilsad Soylu, Ilke Pala-Ozkok, Didem Güven, Goksin Ozyildiz, Berkay Uçan, Güçlü Insel, Emine Cokgor","doi":"10.1080/09593330.2026.2664002","DOIUrl":"https://doi.org/10.1080/09593330.2026.2664002","url":null,"abstract":"<p><p>Nonsteroidal anti-inflammatory drugs (NSAID) are the most frequently observed micropollutants in the effluents of conventional wastewater treatment plants and hybrid technologies could be an alternative to conventional systems. However, the effect on micropollutants' removal has not been well studied. In this study, the impact of hybrid systems on micropollutant removal, as well as the determination of the dominant microbial communities, were investigated. For this purpose, laboratory-scale control hybrid and micropollutant-amended (1 μg/L of diclofenac, ketoprofen, indomethacin, and mefenamic acid, 10 μg/L of ibuprofen, and naproxen) hybrid reactors were operated at organic loading rate of 0.3 kg COD/m³.day and ammonium concentration of 60 mg/L N with a sludge retention time of 10 days and hydraulic retention time of 24 hrs. Effluent chemical oxygen demand (COD) was below 30 mg/L in the control and micropollutant hybrid reactors. Significant removal efficiencies for ibuprofen (99.7%), indomethacin (99.0%), naproxen (97.6%), mefenamic acid (97%), and ketoprofen (91.4) were achieved. Results revealed that NSAIDs did not have a chronic inhibitory effect on the biodegradation of organic matter and nitrification process. Higher biodiversity observed in attached biomass of micropollutant hybrid reactor might contribute to enhance system stability and performance. Dominant genera detected in hybrid micropollutant reactor were <i>Chitinophagaceae</i> (10.1%), <i>Ferruginibacter</i> (7.8%), and <i>Comamonas</i> (6.8%) for suspended biomass, and <i>Fimbriimonadaceae</i> (8.7%), <i>Parafilimonas</i> (6.5%), and <i>Rickettsia</i> (5.6%) for attached biomass, which might contribute to the removal of NSAIDs. Moreover, the genera <i>Chitinophagaceae</i> and <i>Comamonas</i> which are reported as heterotrophic ammonia-oxidizing bacteria, might contribute to nitrification in hybrid biofilm reactors.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-13"},"PeriodicalIF":2.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sustainable synergistic strategy for photocatalytic and <i>Bacillus velezensis</i>-mediated biodegradation of TiO₂-incorporated LDPE with agricultural valorization.","authors":"Divyeshkumar Dave, Vivek Dave, Kunj Patel, Simran Punjabi, Viral Surati, Srusti Gamit, Ankurkumar Khimani, Sunil Chaki, Jatin Patel, Yati Vaidya","doi":"10.1080/09593330.2026.2665433","DOIUrl":"https://doi.org/10.1080/09593330.2026.2665433","url":null,"abstract":"<p><p>Plastic pollution, particularly from low-density polyethylene (LDPE), represents a major global environmental challenge due to its persistence and resistance to natural degradation. In this study, a novel synergistic strategy combining photocatalytic oxidation and microbial biodegradation was developed to enhance LDPE degradation. Rutile titanium dioxide (TiO₂) nanoparticles were synthesised via an eco-friendly sol - gel method and incorporated into LDPE at a concentration of 5 wt% to fabricate photocatalytically active composite films. Plastic-degrading bacteria isolated from plastic-contaminated soil were identified through 16S rRNA gene sequencing as Bacillus velezensis (NCBI Accession: PP499256). The LDPE-TiO₂ composite exhibited significantly enhanced degradation under visible light irradiation, achieving 85% weight loss within 21 days in the presence of B. velezensis, compared with 71% under ultraviolet irradiation and substantially lower degradation in untreated LDPE controls. Fourier Transform Infrared (FTIR) spectroscopy confirmed oxidative degradation through the formation of carbonyl functional groups, while Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified degradation products including acetic acid, propanoic acid, octadecanoic acid, and erythritol, indicating progressive polymer oxidation and microbial metabolism. Furthermore, plant growth experiments using wheat, fenugreek, and green gram demonstrated enhanced germination, root elongation, and chlorophyll content without phytotoxicity. This integrated approach offers a sustainable strategy for plastic waste remediation and valorisation within a circular bioeconomy framework.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-18"},"PeriodicalIF":2.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of magnetite- and attapulgite-modified biochar with organic fertiliser: mineral-driven microbial mechanisms regulating the nitrogen cycle.","authors":"Yuxiang Yao, Qiongyi Cheng, Haibin Zhou, Jingtao Ding, Pengxiang Xu, Pengyue Zhang, Shance Hou, Miao Yu, Peng Han, Dongli Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mismatch between nutrient release from organic fertilisers and crop nitrogen demand often results in low nitrogen use efficiency and substantial nitrogen losses. To address this issue, a pot experiment was conducted to assess the effects of co-applying magnetite or attapulgite modified biochar with organic fertiliser on soil nitrogen transformation, microbial community structure, and rapeseed growth. The results showed that both mineral-modified biochar composites effectively improved soil nitrogen dynamics. The 5% magnetite modified biochar treatment performed best, increasing rapeseed yield to 1200 kg·ha^-1, along with superior plant height, root length, and chlorophyll content. Soil analysis revealed that magnetite enhanced the retention of ammonium and nitrate, whereas attapulgite promoted adsorption and delayed nitrogen release through its porous structure. Microbial analysis showed that magnetite increased the abundance of <i>Bacillota</i> and upregulated ammonia-oxidising bacterial (<i>AOB</i>) genes, thereby enhancing nitrification. In contrast, attapulgite modified biochar regulated the abundance of <i>Pseudomonadota</i> and upregulated the <i>nosZ</i> gene, thereby optimising denitrification and mitigating nitrogen losses. This study provides theoretical and practical insights into the use of mineral modified biochar composites as nitrogen enhancers for sustainable agriculture.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-12"},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic integration of cadmium sulphide and Mn₃O₄ nanozymes on <i>Shewanella oneidensis</i> for photocatalytic hydrogen production with reduced ROS toxicity.","authors":"Mandi Zhu, Siyi Zhang, Huihui Xue, Jialin Zou, Xiaoxue Feng, Weiguang Jie, Lianbao Kan","doi":"10.1080/09593330.2026.2661828","DOIUrl":"https://doi.org/10.1080/09593330.2026.2661828","url":null,"abstract":"<p><p>The integration of semiconductor nanomaterials with electroactive bacteria offers a promising strategy for enhancing light-driven biohybrid systems. However, the generation of reactive oxygen species (ROS) during photocatalysis poses a significant challenge, impairing microbial viability and reducing process efficiency. In this study, we developed a novel biohybrid system by sequentially biosynthesizing cadmium sulphide (CdS) nanoparticles and manganese oxide (Mn₃O₄) nanozyme on the surface of <i>Shewanella oneidensis</i> MR-1, creating an <i>S. oneidensis</i>-CdS@Mn₃O₄ composite. The CdS nanoparticles facilitated efficient light absorption and electron transfer, significantly enhancing hydrogen production under visible light irradiation. However, ROS accumulation (·OH, O₂⁻·, and H₂O₂) induced oxidative stress, reducing bacterial viability and metabolic activity. To address this, Mn₃O₄ nanozyme were introduced, demonstrating robust ROS-scavenging capabilities, reducing hydrogen peroxide (H₂O₂) levels by 66.7%, superoxide radical (O₂⁻·) by 60.7%, and hydroxyl radical (·OH) by a significant margin. As a consequence, hydrogen production by <i>S. oneidensis</i>-CdS@Mn₃O₄ reached 1203.60 µmol g-dcw⁻¹ after 70 h of visible-light irradiation, which was 2.6-fold higher than that of <i>S. oneidensis</i>-CdS. Furthermore, Mn₃O₄ preserved cell viability, maintained higher NADH/NAD⁺ ratios, and enhanced ATP levels, indicating improved metabolic efficiency. Structural characterization via scanning electron microscopy (SEM) energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) confirmed the successful synthesis of CdS and Mn₃O₄ on bacterial surfaces, while photoelectrochemical analysis verified retained photosensitivity. This study presents a simple yet effective strategy for mitigating ROS-induced damage in bio-semiconductor systems, offering insights into the design of more stable and efficient biohybrid platforms for sustainable energy production.Key pointsA biohybrid system (<i>S. oneidensis</i>-CdS@Mn₃O₄) was constructed by sequential biosynthesis of CdS and Mn₃O₄ nanozyme.Mn₃O₄ efficiently scavenged ROS, increasing hydrogen production by 2.6-fold compared to <i>S. oneidensis</i>-CdS.</p>","PeriodicalId":12009,"journal":{"name":"Environmental Technology","volume":" ","pages":"1-17"},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}