European Journal of Oral Sciences最新文献

{"title":"Dental resin composites generate microparticles and impact on gingival keratinocytes-In vitro study.","authors":"Ella Pullinen, Saara V Sirkiä, Johan Nyman, Pekka K Vallittu, Vuokko Loimaranta","doi":"10.1111/eos.70124","DOIUrl":"https://doi.org/10.1111/eos.70124","url":null,"abstract":"<p><p>Along with our increased understanding of the spread of microplastic pollution, the awareness of microparticle release from medical and dental materials has increased. Still, their effects on human health are largely unknown. As the use of resin composite materials in dentistry continues to increase, it is essential to understand the formation and biological activities of microparticles generated during material fabrication and use. This study investigated the type and size of microparticles formed from two commonly used restorative resin composite materials, ground with two different dental burs, and their effects on cultured spontaneously immortalized human gingival keratinocytes. Scanning electron microscopy and laser diffraction were used to examine particle morphology and size distribution. Flow cytometry was used to analyze microparticle adhesion to the cell surface and cell viability, and cytokine release was measured. From the two composite materials, microparticles ranging from approximately 4.50 to 137 µm were released. The particles adhered to gingival keratinocytes and induced IL-8 expression of the cells but did not affect the cell viability. The grinding method and the material affected the size of released particles. Further investigation is needed to understand the impact of cellular responses and the dose dependence of the reactions.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70124"},"PeriodicalIF":2.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148677458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutamate, its relation to periodontitis, diabetes and cognition before and after scaling and root planing.","authors":"Munishwar Rama, Nichani Ashish S, Pai B S Jagadish","doi":"10.1111/eos.70128","DOIUrl":"https://doi.org/10.1111/eos.70128","url":null,"abstract":"<p><p>This study aimed to correlate levels of salivary and serum glutamate in patients with/without generalized periodontitis and Type 2 diabetes mellitus (T2DM) and to evaluate the effect of scaling and root planing (SRP) on glutamate and cognition. We hypothesized that periodontitis and T2DM are associated with altered glutamate levels and poorer cognitive performance and explored whether SRP-related changes in glutamate relate to cognitive performance. Eighty patients aged 45-70-yr were allocated into four groups: healthy, periodontitis, T2DM and periodontitis with T2DM (n = 20 each). Periodontal parameters, Trail Making Tests (TMT-A and TMT-B) and salivary and serum glutamate levels were recorded at baseline and 1-month post-SRP. Diabetic patients showed higher glutamate levels, poorer periodontal status and prolonged TMT times than non-diabetics at baseline. Glutamate levels decreased significantly after SRP, whereas TMT completion times showed only small reductions. Serum glutamate levels showed a significant, moderate positive correlation with TMT-B scores. Fasting plasma glucose and glutamate were significant predictors of periodontitis. Glutamate was altered but did not mediate the periodontitis-TMT association. In T2DM and non-T2DM patients, serum glutamate was the most accurate marker to differentiate periodontitis. SRP improved periodontal status, reduced glutamate levels and was associated with improved executive function and processing speed.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70128"},"PeriodicalIF":2.0,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ evaluation of the bleaching efficacy of experimental hydrogen peroxide hydrogel with MnO-doped Biosilicate activated by violet LED light","authors":"Rafael Dascanio,&nbsp;Marina Trevelin Souza,&nbsp;Camila Siqueira Silva Coelho,&nbsp;Matheus Kury,&nbsp;Edgar Dutra Zanotto,&nbsp;Vanessa Cavalli","doi":"10.1111/eos.70089","DOIUrl":"10.1111/eos.70089","url":null,"abstract":"<p>Hydrogen peroxide (HP) at 35% is effective for dental bleaching but may reduce enamel mineral content and induce structural changes. This study developed a bleaching-hydrogel containing 6%HP and manganese-oxide–doped Biosilicate (MnO_BioS), activated by violet LED, under in situ conditions. Pigmented enamel blocks were attached to palatal devices (<i>n</i> = 12) and kept intraorally for 15 days, being removed only for bleaching sessions. Experimental groups were as follows: 35%HP (positive control) and 6%HP gel containing BioS or MnO_BioS (0/10 wt%), with or without violet LED irradiation. Bleaching consisted of three 30-min sessions with 7-day intervals. Color change (Δ<i>E</i>₀₀), whiteness index (ΔWI_D), color coordinates (Δ<i>L</i>*, Δ<i>a</i>*, Δ<i>b</i>*), surface hardness recovery (%SHR), and enamel inorganic content (CO₃²⁻/PO₄³⁻ ratio by Fourier-transform infrared) were evaluated at baseline (<i>T</i>₀) and after treatment (<i><span>T</span></i>₁), with scanning electron microscopy analysis at <i>T</i>₁. Data were analyzed using analysis of variance and Tukey's-test (<i>α</i> = 0.05). 6%HP_MnO_BioS_LED showed the highest bleaching effectiveness (Δ<i>E</i>₀₀ = 8.90 ± 1.18; ΔWI_D = 20.86 ± 3.26), surpassing 35%HP in ΔWI_D (15.53 ± 1.33) and showing comparable Δ<i>E</i>₀₀ (6.48 ± 1.81). MnO_BioS groups increased enamel CO₃²⁻/PO₄³⁻ ratios after bleaching (0.475 ± 0.02; 0.466 ± 0.01) compared with baseline (0.431 ± 0.01; 0.429 ± 0.01; <i>p</i> &lt; 0.05). Hardness recovery improved (%SHR = 4.14 ± 1.88; 5.45 ± 2.54), whereas it decreased in 35%HP (−1.32 ± 1.67) and 6%HP_LED (−2.60 ± 0.96). 6%HP hydrogel containing MnO_BioS activated by violet LED enhanced bleaching efficacy while preserving enamel mineral-related parameters.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of physical, chemical, and color-matching properties of monochromatic resin composites: An in vitro study","authors":"Ericka dos Santos Lopes,&nbsp;Mayara Evelly Duarte de Lima,&nbsp;Emanuel Ewerton Mendonça Vasconcelos,&nbsp;Natália Gomes de Oliveira,&nbsp;Gabriela Queiroz de Melo Monteiro,&nbsp;Luís Felipe Espíndola-Castro","doi":"10.1111/eos.70081","DOIUrl":"10.1111/eos.70081","url":null,"abstract":"<p>The objectives of this study are to evaluate, in vitro, the microhardness, sorption, solubility, color stability, and color-matching ability of monochromatic resin composites: Palfique Omnichroma / Tokuyama (Mono1) and Vittra APS Unique / FGM (Mono2) compared with the conventional resin composite: Filtek Z250 XT / Solventum (Conv). For this analysis, ten disc-shaped composite specimens were prepared for each group for the microhardness, sorption, solubility, and color stability tests. To evaluate color-matching ability, 36 tooth specimens were obtained from the buccal/lingual surfaces of human molars (<i>n</i> = 12). The teeth were restored, and color compatibility was assessed with a digital spectrophotometer (Vita Easy Shade) and visually. Microhardness and sorption were higher in the Conv group. However, sorption values are within the limits established by ISO 4049/2019 for all groups. No significant difference in solubility or visual color match was noted. However, the conv group demonstrated superior color-matching in the spectrophotometer analysis. After 7 days of coffee immersion, the Mono 1 group showed slightly less pigmentation. Monochromatic resin composites demonstrated physicochemical and organoleptic properties comparable to those of the evaluated conventional resin composites, suggesting similar compatibility and a potential option for simplifying clinical restorative protocols.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of printing orientation and post-curing on optical and surface properties of definitive three-dimensional-printed dental resin with inorganic fillers","authors":"Luana dos Santos Souza,&nbsp;Andrea Mendoza,&nbsp;Taciana Marco Ferraz Caneppele,&nbsp;Pablo Lenin Benitez Sellan,&nbsp;Eduardo Bresciani","doi":"10.1111/eos.70083","DOIUrl":"10.1111/eos.70083","url":null,"abstract":"<p>This study evaluated the influence of printing orientation and post-curing on the color stability, gloss retention, and surface microhardness of a definitive three-dimensional (3D)-printed dental resin, a high-strength photopolymer with inorganic fillers designed for permanent restorations, after immersion in a staining solution. Circular specimens (6 mm diameter and 1.5 mm thickness) were printed at 0°, 45°, and 90° orientations and divided into two groups: control (<i>n</i> = 10), post-cured according to the manufacturer's protocol, and negative control (<i>n</i> = 10), without post-curing. Baseline color (<i>L</i>^*, <i>a</i>^*, <i>b</i>^*), gloss, and Knoop microhardness were measured, followed by reassessment after 21 days of immersion in a staining solution. Data were analyzed using two-way and repeated-measures analysis of variance (<i>α</i> = 0.05). Non-post-cured specimens showed perceptible but clinically acceptable color changes. Microhardness decreased after staining in all groups, regardless of curing. Gloss significantly diminished post-staining, with the control group printed at 45° showing superior retention. Printing orientation influenced color stability, with the 0° orientation showing the highest stability. Post-curing enhanced color stability and gloss retention in definitive 3D-printed resins. Printing orientation affected optical outcomes, emphasizing the importance of optimizing both parameters for long-term esthetic and mechanical reliability.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of sagittal and vertical changes after en masse and two-step retraction using mini-implant anchorage: A randomized controlled trial","authors":"Anam Sattar,&nbsp;Sadia Shabbir,&nbsp;Hana Pervez,&nbsp;Sameer Uddin Shaikh,&nbsp;Sadia Rizwan,&nbsp;Imtiaz Ahmed","doi":"10.1111/eos.70094","DOIUrl":"10.1111/eos.70094","url":null,"abstract":"<p>This parallel two-arm randomized trial assessed skeletal changes following en masse and two-step anterior retraction using mini-implants. Of 110 patients screened, 14 were excluded due to ineligibility or refusal, leaving 96 participants randomized equally into two groups (48 each). All received the assigned intervention. During follow-up, 16 participants were lost because of bracket debonding, mini-implant failure, or discontinuation, resulting in 80 subjects (40 per group) included in the final analysis. All patients were treated with pre-adjusted edgewise appliances. After alignment and leveling, bilateral mini-implants were inserted between the maxillary second premolars and first molars, followed by extraction of the maxillary first premolars. Retraction was carried out according to group allocation either en masse or two-step. Lateral cephalograms taken before treatment and after incisor retraction were used to evaluate skeletal changes. Data were expressed as mean and standard deviation, with Wilcoxon signed-rank and Mann–Whitney <i>U</i>-tests applied for intra- and intergroup comparisons. Both methods produced significant sagittal and vertical changes. Although the two-step approach showed slightly greater maxillary retraction and ANB reduction, these differences likely related to baseline variation and biomechanics rather than true superiority. Vertical parameters increased in both groups, indicating posterior mandibular rotation, suggesting both techniques are equally effective.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absence of DNA viruses in ameloblastomas","authors":"Maria K. Jauhiainen,&nbsp;Jetta Kelppe,&nbsp;Maria Söderlund-Venermo,&nbsp;Antti A. Mäkitie,&nbsp;Saku T. Sinkkonen,&nbsp;Jaana Hagström","doi":"10.1111/eos.70092","DOIUrl":"10.1111/eos.70092","url":null,"abstract":"<p>Ameloblastomas (ABs) are rare odontogenic benign tumors. Although studies of the molecular pathogenesis have revealed the presence of mutations and altered gene expression, the etiology is unresolved. Viruses cause 10%–15 % of cancers, but their potential role within benign tumors is less studied. This study focuses on the potential presence of herpes-, parvo-, and polyomaviruses in ABs. Thirty DNA viruses comprising herpes-, parvo-, and polyomaviruses were analyzed by qPCR and Luminex-based methods in 11 formalin-fixed paraffin-embedded AB samples. In repeated analyses, the ABs harbored none of the studied large set of viruses. Based on our findings a relevant role of the studied viruses seems unlikely in the pathogenesis of ABs.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147644554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of in-office bleaching on the color stability of bulk-fill resin composites immersed in staining beverages","authors":"Matheus Kury,&nbsp;Thaís T. Feldens,&nbsp;Márcia V. G. B. Queiroz,&nbsp;Mayara A. P. Oliveira,&nbsp;Luciano S. Gonçalves,&nbsp;Maria Carolina G. Erhardt","doi":"10.1111/eos.70097","DOIUrl":"10.1111/eos.70097","url":null,"abstract":"<p>This study evaluated the impact of in-office bleaching on color stability of bulk-fill composites submitted to staining. Bulk-fill (Aura, Opus, and Filtek One) and nanofilled (Filtek Z350) composite specimens were submitted to the following: control (CT); bleaching and immersion in distilled water (BL); and bleaching with daily 15-min immersion in coffee (BL + CF), cola-based drink (BL + CO), or red wine (BL + RW) among sessions. A spectrophotometer determined Δ<i>E</i>₀₀, ΔWI_D, and coordinates. Degree of conversion (DC) and topography of the composites were assessed using Fourier-transform infrared spectroscopy in attenuated total reflectance (FTIR-ATR) and scanning electron microscopy (SEM), respectively. Quantitative data were submitted to statistical tests according to the factors (<i>α</i> = 5%). In BL, Opus and Aura displayed significantly higher Δ<i>E</i>₀₀ and ΔWI_D than One. Compared to BL, only BL + CF significantly decreased the ΔWI_D and Δ<i>L</i> for Aura, but all staining immersions decreased ΔWI_D and Δ<i>L</i> for Opus. One and Z350 were not impacted by treatments, except for BL + CF, which significantly decreased ΔWI_D and Δ<i>L</i>. SEM revealed influence of cycling on composites’ surface, but DC did not determine their staining resistance. In-office bleaching compromised bulk-fill composite color stability, with Aura and Opus showing unacceptable changes, whereas Filtek One and Z350XT remained acceptable. Staining challenges altered Opus stability, and coffee immersion affected colorimetric outcomes across all composites after bleaching.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-curing effects on mechanical properties staining resistance of a 3D-printed resin versus a direct composite for definitive restorations","authors":"Bianca Rossi,&nbsp;Arthur Henrique Marques Massarenti,&nbsp;Giulia Gamero Pizzanelli,&nbsp;Márcia Valéria Gualberto Barbosa de Queiroz,&nbsp;Filipe Milazzo dos Santos,&nbsp;Matheus Kury,&nbsp;Adriano Fonseca Lima","doi":"10.1111/eos.70093","DOIUrl":"10.1111/eos.70093","url":null,"abstract":"<p>This study evaluated the mechanical performance and staining resistance of a three-dimensional (3D)-printed resin (VarseoSmile Crown Plus) subjected to different post-curing times (0, 5, 10, 15, and 20 min), compared with a conventional nanofilled resin composite (Filtek Z350XT). Specimens of both materials were fabricated and tested for flexural strength and flexural modulus using a three-point bending test, whereas cohesive strength (CS) was assessed by microtensile testing. For color analysis, disk specimens were immersed in artificial saliva, Coca-Cola, or red wine (RW) for 7 days, followed by polishing and whitening procedures. Color change (Δ<i>E</i>₀₀) and whiteness index (ΔWI_D) were recorded. Data were analyzed using a generalized linear model and Bonferroni tests (<i>α</i> = 5%). The direct composite showed higher flexural and CS than the 3D-printed resin. Post-curing improved the mechanical properties of the printed material, although values remained inferior to those of the composite. Both materials exhibited color changes after staining, with RW causing the greatest discoloration. Polishing and whitening reduced staining but did not always restore baseline values. Although post-curing enhances 3D-printed resin performance, its mechanical behavior and color stability remain inferior to those of conventional composites, indicating the need for further optimization before clinical use as definitive restorations.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infrared thermographic evaluation of root surface during warm vertical compaction technique","authors":"Aysenur Oncu,&nbsp;Ecem Ozgur,&nbsp;Merve Sarı,&nbsp;Pelin Tufenkci,&nbsp;Berkan Celikten","doi":"10.1111/eos.70082","DOIUrl":"10.1111/eos.70082","url":null,"abstract":"<p>This study aimed to evaluate the effects of heat setting, plugger size, and dentin thickness on the temperature increase at the root surface during warm vertical compaction. A total of 96 mandibular premolars, 36 maxillary, and 36 mandibular central incisors were selected. All root canals were prepared using a 30/07 file. AH Plus sealer and gutta-percha were placed into the canals. In the mandibular premolars, small (40/0.025), medium (50/0.05), and large (60/0.06) pluggers were used, each set at temperatures of 180, 200, 220, and 250°C. For the maxillary and mandibular incisors, a medium-sized plugger was used across all heat settings. Heat was applied to each sample for 3 s, and the maximum temperature was recorded during warm vertical compaction of gutta-percha using an infrared thermal camera. Multiple-linear regression models with dummy variables were utilized to estimate the predictive influence of heat settings, plugger sizes, and dentine thickness on root surface temperature. At 200 and 220°C, the large plugger was associated with a significantly greater temperature increase compared to the small plugger. However, no significant interaction was observed between 250°C setting and pluggers. Interaction between dentin thickness and heat setting indicated that thin dentin was associated with greater temperature increases at 220 and 250°C.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}