Epigenetic markers of tooth eruption – DNA methylation and histone acetylation

IF 1.8 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

European Journal of Oral Sciences Pub Date : 2024-07-16 DOI:10.1111/eos.13005

Anna Westerlund, Asal Shikhan, Nina Sabel, Farah Asa'ad, Lena Larsson

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Abstract

The present study aimed to evaluate whether epigenetic markers are expressed in the dental follicles surrounding ectopically erupting teeth. Twenty-one dental follicles were collected in 20 adolescent children through surgical exposure of ectopic teeth. The epigenetic modifications of DNA methylation and histone acetylation were evaluated by immunohistochemistry. The results showed cells positive for DNA-methyltransferase 1 (DNMT1), DNA methyltransferase 3 beta (DNMT3B), ten-eleven translocation-2 (TET2), acetyl-histone H3 (AcH3), acetyl-histone H4 (AcH4), 5-methylcytosine (5mC), and 5-hydroxymethylcytosine (5hmC) were present in all the samples. The levels of epigenetic markers representing active chromatin (5hmC, AcH3, AcH4, and TET2) were statistically significantly higher than those of markers representing inactive chromatin (5mC, DNMT3B, DNMT1). In conclusion, follicles in ectopic teeth display major epigenetic modifications. In the follicles, epigenetic markers associated with the activation of bone-related genes are more abundant than markers associated with the inactivation of bone-related genes.

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牙齿萌出的表观遗传标记--DNA 甲基化和组蛋白乙酰化。

本研究旨在评估异位萌出牙周围的牙囊是否表达表观遗传标记。研究人员通过手术暴露异位牙齿，收集了 20 名青少年儿童的 21 个牙囊。通过免疫组织化学方法对 DNA 甲基化和组蛋白乙酰化的表观遗传修饰进行了评估。结果显示，所有样本中都存在 DNA-甲基转移酶 1 (DNMT1)、DNA 甲基转移酶 3 beta (DNMT3B)、十-十一易位-2 (TET2)、乙酰组蛋白 H3 (AcH3)、乙酰组蛋白 H4 (AcH4)、5-甲基胞嘧啶 (5mC) 和 5-羟甲基胞嘧啶 (5hmC) 阳性的细胞。在统计学上，代表活性染色质的表观遗传标记（5hmC、AcH3、AcH4 和 TET2）的水平明显高于代表非活性染色质的标记（5mC、DNMT3B 和 DNMT1）。总之，异位牙中的毛囊显示出重大的表观遗传修饰。在滤泡中，与骨相关基因激活相关的表观遗传标记比与骨相关基因失活相关的标记更为丰富。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Oral Sciences 医学-牙科与口腔外科

CiteScore

3.50

自引率

5.30%

发文量

审稿时长

2 months

期刊介绍： The European Journal of Oral Sciences is an international journal which publishes original research papers within clinical dentistry, on all basic science aspects of structure, chemistry, developmental biology, physiology and pathology of relevant tissues, as well as on microbiology, biomaterials and the behavioural sciences as they relate to dentistry. In general, analytical studies are preferred to descriptive ones. Reviews, Short Communications and Letters to the Editor will also be considered for publication. The journal is published bimonthly.