{"title":"Alteration in oral and non-oral tissues in ligature-induced periodontitis mice with the Alzheimer's disease risk factor APOE4","authors":"Ae Ri Kim, Yun-Jung Yoo, Eun-Jung Bak","doi":"10.1111/eos.70091","DOIUrl":"10.1111/eos.70091","url":null,"abstract":"<p>The <i>APOE4</i> gene, particularly the ε4 allele, is linked to susceptibility to Alzheimer's disease, and periodontitis can cause pathological changes in multiple organs, but combined effects of these conditions and the impact of tumor necrosis factor (TNF)-α regulation on the combined effects remain unclear. We examined the alterations in oral and non-oral tissues in <i>APOE4</i>-knockin mice with periodontitis and evaluated the effects of infliximab, a TNF-α inhibitor. Mice were grouped as control, periodontitis, <i>APOE4</i>-knockin, <i>APOE4</i>-knockin with periodontitis, infliximab-treated periodontitis, and infliximab-treated <i>APOE4</i>-knockin with periodontitis. There were no differences in alveolar bone volume and periodontal inflammatory cells among all periodontitis-induced groups. The <i>APOE4</i>-knockin with periodontitis group showed a decrease in hippocampal Cornu Ammonis 1 neurons (<i>p</i> < 0.05) and increased renal and hepatic fibrosis (<i>p</i> < 0.05) compared to control group. The infliximab-treated <i>APOE4</i>-knockin with periodontitis group had even greater neuronal loss (<i>p</i> < 0.001) and renal fibrosis (<i>p</i> < 0.01). Glial fibrillary acidic protein mRNA expression, a marker of astrocyte activation, was higher in the infliximab-treated <i>APOE4</i>-knockin with periodontitis group than in the control group. These suggest that <i>APOE4</i> with periodontitis worsens neuronal loss and renal and hepatic fibrosis, and TNF-α inhibition in this coexistence may have harmful effects on both the brain and kidney.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147632902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Remarkable decline and sustained low levels of caries in the Nordic populations—Explanations and implications for public oral health","authors":"Bente Nyvad, Ole Fejerskov","doi":"10.1111/eos.70114","DOIUrl":"10.1111/eos.70114","url":null,"abstract":"<p>Community water fluoridation has been acclaimed as an effective mode of caries control at the population level. However, a recent review has concluded that in high-income populations with widespread access to topical fluorides, water fluoridation (0.7 ppm F) has a minor effect on the development of caries. It may therefore be relevant to take a historical look at the benefits of an oral health intervention that does not rely on the use of systemic fluorides, such as toothbrushing with fluoride toothpaste. We summarize the evidence from large population studies in the Nordic countries during the past 50-yr and show that it is possible to obtain and sustain low caries levels over decades by self-performed use of fluoride toothpaste. Topical fluoride at low concentrations <i>treats/arrests</i> active/progressing caries lesions by controlling mineral loss in dental hard tissues, thereby reducing the development of cavities. Toothbrushing with fluoride toothpaste shifts the entire caries distribution to the left, including the right-hand tail end of the distribution, ensuring that also the more disadvantaged groups may benefit. Daily brushing with fluoride toothpaste should be promoted and trained through school-based oral health programs for children. It is time for a reappraisal of fluoride toothpaste for public oral health.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148224050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stefan Vulović, Bojana Stanisavljev, Nikola Živković, Dušan V. Milojkov, Gvozden Jovanović, Nina Tomić, Aleksandra Milić Lemić
{"title":"Marginal adaptation and microleakage of resin-based composite provisional crowns fabricated by different techniques and materials","authors":"Stefan Vulović, Bojana Stanisavljev, Nikola Živković, Dušan V. Milojkov, Gvozden Jovanović, Nina Tomić, Aleksandra Milić Lemić","doi":"10.1111/eos.70086","DOIUrl":"10.1111/eos.70086","url":null,"abstract":"<p>The aim of this study was to evaluate the marginal adaptation and microleakage of resin-based composite provisional crowns fabricated using different techniques and materials and cemented with different temporary cements. One hundred twenty extracted molars prepared for zirconia crowns were allocated to three fabrication groups (<i>n</i> = 40): conventionally produced (CONV), computer-aided design and computer-aided manufacturing-milled (MILL), and three-dimensional-printed (PRNT). Each group was subdivided according to temporary cement type (<i>n</i> = 20): zinc oxide non-eugenol (ZONE) or dual-cure resin-based (DCRB) cement. Specimens underwent thermocycling for 500 (T1) (<i>n</i> = 10) or 5000 cycles (T2) (<i>n</i> = 10). Marginal adaptation was evaluated using scanning electron microscopy (<i>n</i> = 5), whereas microleakage was assessed by methylene blue dye penetration (<i>n</i> = 5). Statistical analysis included two-way analysis of variance with Tukey post hoc test for marginal adaptation and Kruskal–Wallis with Dunn's test for microleakage. PRNT and MILL crowns demonstrated smaller marginal gaps and lower microleakage scores than CONV crowns at both aging stages. DCRB cement produced smaller gaps and equal or lower leakage than ZONE across different material groups. All groups exhibited marginal discrepancies below the clinically acceptable limit of 120 µm. PRNT crowns cemented with DCRB demonstrated the most favorable marginal adaptation and sealing ability.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyung Su Shin, Han Wool Kwak, Jong-Seong Chae, Si-On Choi, Suah Han, Hwa Young Yu, Dae Ho Leem, Hyun Seok
{"title":"Effect of aging on healing of mandibular tooth extraction socket in a rat model","authors":"Kyung Su Shin, Han Wool Kwak, Jong-Seong Chae, Si-On Choi, Suah Han, Hwa Young Yu, Dae Ho Leem, Hyun Seok","doi":"10.1111/eos.70099","DOIUrl":"10.1111/eos.70099","url":null,"abstract":"<p>This study evaluated the effect of aging on bone regeneration in mandibular extraction sockets. Male Sprague–Dawley rats were divided into three age groups: 2-month-old (2 M, <i>n</i> = 6), 10-month-old (10 M, <i>n</i> = 6), and 18-month-old (18 M, <i>n</i> = 5). Two weeks post-extraction, comprehensive analyses were performed using micro-computed tomography (<i>µ</i>-CT), serum bone turnover markers, histology, and immunohistochemistry. Notably, to ensure accurate volumetric assessment, a specific tooth-based region of interest utilizing the extracted tooth was employed for precise <i>µ</i>-CT analysis. Radiologically, the 18 M group exhibited significantly reduced bone volume and trabecular thickness compared to the younger groups. Serum analysis revealed that younger rats maintained higher baseline metabolic activity, specifically regarding osteocalcin and CTX-I, and exhibited a distinct post-extraction tartrate-resistant acid phosphatase 5b response. Histological and immunohistochemical findings corroborated these results; the 10 M group demonstrated the most favorable bone regeneration with maximal alkaline phosphatase expression filling the socket, whereas the 18 M group exhibited compromised healing characterized by empty sockets and inflammatory cell accumulation. In conclusion, aging significantly deteriorates the biological healing capacity of alveolar bone. Although younger animals exhibit robust metabolic responses and bone formation, senescent rats show a marked decline in regenerative potential, emphasizing the need for age-specific considerations in dental rehabilitation.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":"134 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eos.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sri Laxmi Bn, Renilda Berylswan, Sushmita Batni, Namratha Nayak
{"title":"The emerging role of piRNAs in periodontal and orthodontic tissue dynamics: Biological insights and clinical perspectives-A review.","authors":"Sri Laxmi Bn, Renilda Berylswan, Sushmita Batni, Namratha Nayak","doi":"10.1111/eos.70122","DOIUrl":"https://doi.org/10.1111/eos.70122","url":null,"abstract":"<p><p>Inflammatory conditions of teeth and their surrounding structure lead to tissue destruction and alveolar bone loss. This review critically examines the emerging role of PIWI-interacting RNAs (piRNAs) in periodontology and orthodontic tooth movement focusing on their involvement in inflammation, bone remodeling, and cellular regulation with periodontal ligament and alveolar bone. Although piRNAs were initially characterized in reproductive biology, recent studies suggest their involvement in fundamental biological basis: Both involve inflammatory responses, bone remodeling, and the regulation of cellular activities within the periodontal ligament and alveolar bone. Traditionally, research has focused on protein-coding genes, cytokines, and signaling molecules. However, the emerging role of noncoding RNAs (ncRNAs), including microRNAs, long ncRNAs, and piRNAs, is revolutionizing the understanding of oral biology. This review highlights key findings: identification of piRNA's with relevance to oral biology (piR-63049 and piR-36741); their association with Wnt/β-catenin and BMP2/METTL3-m6A pathways; evidence supporting piRNAs as potential biomarkers in saliva/gingival crevicular fluid; and the translational potential of exosome-mediated piRNA delivery for periodontal regeneration and orthodontic modulation. piRNAs thus represent a novel regulatory component, and their potential use as diagnostic biomarkers and therapeutic targets warrants the need for further investigation to establish their functional relevance in oral health and disease.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70122"},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The speed of innovation versus the speed of validation: A growing governance gap in contemporary endodontics.","authors":"Mohammed Turky, Paul M H Dummer","doi":"10.1111/eos.70119","DOIUrl":"https://doi.org/10.1111/eos.70119","url":null,"abstract":"<p><p>Endodontics has witnessed a significant acceleration in innovation. However, the pace at which these innovations are validated through scientific research has not kept up, creating what is referred to as the 'speed of innovation versus speed of validation gap'. This incongruence raises critical concerns about the foundation of evidence-based practice, patient safety and professional accountability. Currently, many innovations are adopted in clinical practice based on preliminary data, anecdotal evidence or popularity on digital platforms, often before thorough clinical validation is achieved. This trend challenges traditional knowledge translation frameworks and reveals significant limitations within existing governance models in dentistry. This article draws on principles of responsible innovation and healthcare regulations to examine the factors contributing to this gap in endodontics and its implications. Although recognising the necessity of innovation for the advancement of the discipline, it cautions against the uncritical and premature adoption of new techniques and technologies, as this may jeopardise clinical outcomes and erode public trust. The article advocates for a more integrated approach that combines innovation with anticipatory governance, critical appraisal and professional reflexivity. Closing this gap is imperative to ensure that advancements in endodontics are founded on robust scientific evidence and conducted in a socially responsible manner.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70119"},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vitória Lacerda Santos, Luan Júlio Ruiz da Silva, Antônia Patrícia Oliveira Barros, Mileide da Paz Brito, Rafael Rodrigues Lima, Cristiane de Melo Alencar, Milton Carlos Kuga
{"title":"Effect of bioactive dentin modulators on adhesion of glass fiber posts.","authors":"Vitória Lacerda Santos, Luan Júlio Ruiz da Silva, Antônia Patrícia Oliveira Barros, Mileide da Paz Brito, Rafael Rodrigues Lima, Cristiane de Melo Alencar, Milton Carlos Kuga","doi":"10.1111/eos.70121","DOIUrl":"https://doi.org/10.1111/eos.70121","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of natural dentin modulators on the bond strength and adhesive interface of resin materials bonded to dentin oxidized by sodium hypochlorite. Forty bovine roots underwent endodontic treatment and were assigned to four groups: control gel, Libidibia ferrea 10% gel, Apis mellifera 10% gel, and sodium hypochlorite irrigation. Glass fiber posts were cemented using a universal adhesive system and dual-cure resin cement. Bond strength was assessed by the push-out test, and the failure pattern and resin tag penetration were also evaluated. The results showed that both L. ferrea and A. mellifera treatments enhanced bond strength and resin tag extension compared to the control and sodium hypochlorite groups, with no difference between the two natural modulators, regardless of the root canal third. Cohesive failures were more frequent in the natural modulator groups, whereas adhesive failures predominated in the control and sodium hypochlorite groups. The findings indicate that natural dentin modulators improved the adhesive performance of resin materials bonded to dentin previously oxidized by sodium hypochlorite.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70121"},"PeriodicalIF":2.0,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148351223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdulaziz Alshehri, Abdulrahman Alshabib, Yasser Alfawaz, Abdulaziz Alayed, Nassr Almafleh, Nick Silikas, Mohammed AlRabiah, Hamad Algamaiah
{"title":"Deep-layer integrity in bulk restorations: Effects of placement technique on fiber- and particulate-reinforced bulk-fill composites properties.","authors":"Abdulaziz Alshehri, Abdulrahman Alshabib, Yasser Alfawaz, Abdulaziz Alayed, Nassr Almafleh, Nick Silikas, Mohammed AlRabiah, Hamad Algamaiah","doi":"10.1111/eos.70117","DOIUrl":"https://doi.org/10.1111/eos.70117","url":null,"abstract":"<p><p>This study investigated the influence of bulk-fill versus incremental placement techniques on the mechanical properties of the bottom layer of fiber- and particulate-reinforced bulk-fill resin composites following long-term water storage. Five materials were evaluated: a conventional composite, a high-viscosity bulk-fill, a fiber-reinforced flowable bulk-fill, and two particulate-reinforced flowable bulk-fills. Specimens were prepared using a custom mold to simulate clinically relevant placement techniques, either incremental or bulk-fill techniques. Specimens stored in distilled water for either 24 h or 30 d. The incremental technique produced similar flexural strength between top and bottom layers, whereas the bulk-fill technique showed higher top-layer strength, except for the fiber-reinforced composite, which maintained comparable performance across layers and conditions. Long-term storage reduced differences among bulk-fill materials. Hardness was generally higher in packable composites, with the fiber-reinforced material outperforming other flowable types. Extended water storage only reduced the hardness of the conventional composite. The fiber-reinforced bulk-fill composite exhibited stable and superior mechanical performance under all conditions, whereas particulate packable composites remained the strongest overall. Incremental placement improved deep-layer properties in particulate bulk-fill composites.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70117"},"PeriodicalIF":2.0,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Does the 2018 periodontal classification improve patient understanding? A missing piece in periodontal care.","authors":"Clovis Mariano Faggion","doi":"10.1111/eos.70120","DOIUrl":"https://doi.org/10.1111/eos.70120","url":null,"abstract":"<p><p>The 2018 AAP/EFP Classification of Periodontal Diseases introduced staging and grading to improve the description of periodontitis severity and factors associated with disease progression. Although widely adopted in clinical and research settings, its validation has focused mainly on inter-examiner agreement, diagnostic concordance and prognostic performance, with limited evidence on patient-centred outcomes such as comprehension, recall and behavioural relevance. Available studies suggest that patients retain staging and grading poorly and instead rely on observable and functional indicators of disease, indicating a potential mismatch between clinical constructs and patient-relevant information for decision-making. This article examines the 2018 classification from a communication perspective, distinguishing diagnostic validity from communicative validity and evaluating whether extent, stage and grade can meaningfully support patient understanding. A pragmatic conceptual model reframes these constructs as communication components describing disease distribution, current burden and progression risk. Overall, although the 2018 system provides a structured framework for periodontal classification, its effectiveness as a communication tool remains uncertain. Future research should investigate how periodontal information can be structured to improve comprehension, recall and informed decision-making in practice.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70120"},"PeriodicalIF":2.0,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Esthetic and adhesive properties of a novel colored fiber post: A restorative approach to an endodontic challenge.","authors":"Laís Lima Pelozo, Diana Codas-Duarte, Camila Tirapelli, Manoel Damião Sousa-Neto, Aline Evangelista Souza-Gabriel","doi":"10.1111/eos.70118","DOIUrl":"https://doi.org/10.1111/eos.70118","url":null,"abstract":"<p><p>Colored fiber posts facilitate clinical retrieval but could impact esthetics and bonding. This study evaluated the color stability and dentin bond strength of a novel colored fiber post designed to facilitate post-removal. Sixty premolars had root canal therapy and post space preparation. Color parameters (ΔL*, Δa*, Δb*) were measured with a spectrophotometer. Specimens were divided into two groups: control-Exacto post (Angelus); experimental-blue-colored fiber post (patent granted). A flowable resin was used for core build-up, and restoration was done with a composite resin (shade A2). Overall color change (ΔE*ab) and whiteness index variation (ΔWI) were calculated (n = 10). Push-out bond strength was assessed at 24 h (n = 10) and 12 months (n = 10). Data were analyzed using anova, Tukey's post hoc, and chi-square tests (α = 0.05). No significant differences were found between groups for ΔE*ab or ΔWI. After aging, both groups showed increased ΔE*ab and ΔWI. Bond strength was similar between groups, although both decreased over 12 months. Adhesive failure was predominant in both groups. The experimental colored fiber post demonstrated esthetic and mechanical performance comparable to a conventional fiber post, offering a potential alternative for restoring endodontically treated teeth with enhanced retreatability and safety.</p>","PeriodicalId":11983,"journal":{"name":"European Journal of Oral Sciences","volume":" ","pages":"e70118"},"PeriodicalIF":2.0,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}