European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Investigation of clonal relationship in hospital-associated Candida parapsilosis isolates.","authors":"Mahmut Cem Ergon, Ebru Demiray Gürbüz, Nazlı Arslan, Sema Alp, Mine Doluca Dereli, Ayşe Aydan Özkütük","doi":"10.1007/s10096-024-04998-2","DOIUrl":"10.1007/s10096-024-04998-2","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to investigate the clonal relationship and antifungal susceptibility of C. parapsilosis isolated from hospitalized patients and to determine whether it is due to transmission or not and the spread status of resistant isolates.</p><p><strong>Methods: </strong>Between January 2017 and June 2019, totally 277 C parapsilosis isolated from blood, urine and catheter samples of adult or pediatric in-patient (intensive care and service) who applied to Mycology laboratory in our hospital were included in the study. All isolates were identified using conventional methods, API 20 C AUX (Biomerieux, France) semi-automated system and confirmed by MALDI-TOF MS Biotyper Smart (Bruker Daltonik GmbH, Germany). Randomly amplified polymorphic DNA (RAPD) PCR method was used for molecular genotyping of isolates. MIC values for fluconazole, anidulafungin and amphotericin B were determined according to the M27-A3 CLSI broth microdilution reference method guideline.</p><p><strong>Results: </strong>Seven different band patterns (A-G) were detected in 277 isolates by RAPD PCR method. According to the rank order of the isolates, 170 (61.37%) C, 65 (23.47%) A, 18 (6.50%) G, 11 (3.97%) B, six (2.17%) E, two (0.72%) F and one (0.36%) D patterns were determined. When the band patterns of the isolates were evaluated according to the years, it was detected that C pattern continued between 2017 and 2019 and that all isolates continued to spread only as C pattern in 2019. While 211 (76.17%) of the isolates were resistant to fluconazole (≥ 8 µg/ml), two (0.72%) were resistant to amphotericin B (≥ 2 µg/ml) and two (0.72%) were intermediate to anidulafungin.</p><p><strong>Conclusions: </strong>It is noteworthy that the spread of the C pattern in C. parapsilosis strains has increased over the years and is the main pattern isolated from the whole hospital. The detection of high fluconazole resistance in C. parapsilosis isolates in our hospital may also be related to the dominant pattern.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"311-322"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in the growing impact of group A Streptococcus infection on public health after COVID-19 pandemic: a multicentral observational study in Okayama, Japan.","authors":"Shinnosuke Fukushima, Takashi Saito, Yoshitaka Iwamoto, Yuko Takehara, Haruto Yamada, Koji Fujita, Masayo Yoshida, Yasuhiro Nakano, Hideharu Hagiya","doi":"10.1007/s10096-024-05015-2","DOIUrl":"10.1007/s10096-024-05015-2","url":null,"abstract":"<p><strong>Purpose: </strong>Following the COVID-19 pandemic, group A Streptococcus (GAS) infection has been surging worldwide. We aimed to compare the disease burden between notified cases of streptococcal toxic shock syndrome (STSS) and unreported GAS infections.</p><p><strong>Methods: </strong>This is a multicentral observational study, retrospectively performed at seven hospitals in Okayama prefecture in Japan from January 2022, to June 2024. Clinical and microbiological data of patients with positive cultures of GAS were collected from the medical records. Primary outcomes were defined as rates of surgical procedures, intensive care unit (ICU) admission, and in-hospital mortality, which were compared among patients with locally-defined STSS, invasive GAS (iGAS), and non-iGAS infection.</p><p><strong>Results: </strong>GAS was detected in 181 patients, with 154 active cases of GAS infection. The number of patients with GAS infection surged in late 2023. The most common source of infection was skin and soft tissue infections, accounting for 83 cases, including 15 cases of necrotizing fasciitis, and 12 cases (7.8%) were notified to public health authorities as STSS. Among the 25 unreported iGAS cases, 9 (36.0%) underwent surgical intervention, and 4 patients (16.0%) required ICU admission. The mortality rates in the unreported iGAS cases were comparable to those observed in the notified STSS.</p><p><strong>Conclusions: </strong>We highlighted that the number of iGAS infections was twofold higher than that of notified STSS, with comparable mortality rate between these groups, indicating substantial underestimation of the true burden of iGAS. This epidemiological investigation has significant implications for enhancing infectious disease surveillance frameworks and public health policy development.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"383-391"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rectal colonization with multidrug-resistant organisms and risk for bloodstream infection among high-risk Greek patients.","authors":"Polyxeni Karakosta, Georgios Meletis, Elisavet Kousouli, Efthymia Protonotariou, Aikaterini Tarpatzi, Sophia Vourli, Panagiota Christina Georgiou, Vasiliki Mamali, Lemonia Skoura, Olympia Zarkotou, Spyros Pournaras","doi":"10.1007/s10096-024-04987-5","DOIUrl":"10.1007/s10096-024-04987-5","url":null,"abstract":"<p><strong>Background: </strong>Studies link multidrug-resistant organism (MDRO) rectal colonization to increased infection risk, data from Greece, a country with high rates of MDRO, are limited.</p><p><strong>Methods: </strong>We assessed bloodstream infection (BSI) risk following rectal colonization by MDROs across three Greek hospitals (2019-2022).</p><p><strong>Results: </strong>Of 4,370 inpatients, 31.1% were colonized by carbapenem-resistant Enterobacterales (CRE), 30.1% carbapenem-resistant Acinetobacter baumannii (CRAB), 5.8% carbapenem-resistant Pseudomonas aeruginosa (CRPA), 28.4% vancomycin-resistant enterococci (VRE). Subsequent BSI from the same MDRO species was developed in 15.6% of CRE, 19.7% of CRAB, 9.2% of CRPA and 3.5% of VRE carriers. Previous rectal colonization increases significantly MDRO BSI risk [RR (95%CI): CRE 5.2 (3.9-6.8), CRAB 2.7 (2.2-3.3), CRPA 9.6 (5.8-16.0), VRE 2.5 (1.5-4.2)].</p><p><strong>Conclusion: </strong>Clinicians should consider MDRO carriage information for selecting empiric treatment.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"437-442"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Epidemiology of Pseudomonas aeruginosa Sequence Type 111.","authors":"Yasufumi Matsumara, Gisele Peirano, Marleen Kock, Johann D D Pitout","doi":"10.1007/s10096-024-05010-7","DOIUrl":"10.1007/s10096-024-05010-7","url":null,"abstract":"<p><strong>Purpose: </strong>Pseudomonas aeruginosa ST111 is a global multidrug resistant (MDR) high-risk clone and comprehensive data about its molecular epidemiology is limited in Canada. Comprehensive data about the evolution of ST111 clades is limited. We characterized a Canadian collection of ST111 causing bloodstream infections and investigated the genomic relationship between Canadian and global ST111.</p><p><strong>Material and methods: </strong>We used long and short read WGS to characterize Canadian ST111 (n = 10 from 2010-18). We performed phylogenetic analysis on a global collection of ST111 (n = 969) and investigated the evolutionary history of clades using BEAST.</p><p><strong>Results: </strong>ST111 belonged to 3 clades (A, B, C) and two subclades (C1, C2). ST111-A was the ancestral clade while clades B, C1 and C2 emerged during the 1700s and 1800s. ST111-C2 dominated the global ST111 population. Serotype switching from O4 to O12 and the acquisition of Tn21, gyrA_T83I, parC_S87L, In59 with bla_VIM-2 and aacA29 over time, were important in the evolution of ST111-C2. The Calgary ST111 strains consisted of a diverse collection that belonged to ST111-A (O4), ST111-C1 (O4) and ST111-C2 (O12) with different transposon structures.</p><p><strong>Conclusions: </strong>We provided details on the emergence and evolution of different ST111 clades over time and highlighted the roles of serotype switching and the acquisition of certain AMR determinants and transposon structures in the evolution of ST111-C2.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"375-381"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of fluconazole use on the fungal and bacterial microbiomes in recurrent Vulvovaginal Candidiasis (RVVC): a pilot study of vaginal and gastrointestinal site interplay.","authors":"Moira Bradfield Strydom, Tiffanie M Nelson, Sohil Khan, Ramesh L Walpola, Robert S Ware, Evelin Tiralongo","doi":"10.1007/s10096-024-04999-1","DOIUrl":"10.1007/s10096-024-04999-1","url":null,"abstract":"<p><strong>Purpose: </strong>Recurrent Vulvovaginal Candidiasis (RVVC) is a problematic clinical condition for which fluconazole treatment is commonly prescribed. This study investigated the interkingdom vaginal and gastrointestinal microbiomes of RVVC patients who use fluconazole intermittently or as longer-term maintenance therapy for symptom management and compared them to healthy controls.</p><p><strong>Methods: </strong>Vaginal swabs and fecal samples were collected. A novel interkingdom analysis was performed using 16 S rRNA and ITS1 gene sequencing to compare the diversity and taxonomic composition of vaginal microbiome (VMB) and gastrointestinal microbiome (GIMB).</p><p><strong>Results: </strong>Twenty-seven women participated: 10 intermittent users and healthy controls and 7 maintenance therapy. The study revealed that microbiomes of fluconazole users do not differ in diversity metrics from healthy controls. RVVC patients using intermittent fluconazole displayed a higher abundance of vaginal C. albicans than healthy controls. Candida species pairings were not commonly observed between sites in individuals and, as such a fecal reservoir is unlikely to be implicated in recurrent symptomatology. In many of the RVVC non-Candida fungal spp. were identified in the vaginal microbiome. Users of fluconazole displayed elevations of the CST-I (Community State Type 1) associated bacterium L. crispatus. All participants displaying vaginal Candida spp. belonged to either bacterial CST-I or CST-III (Community State Type 3- L. iners associated).</p><p><strong>Conclusion: </strong>To our knowledge, this is the first study to compare the interkingdom VMB-GIMB of women with RVVC using oral fluconazole. As fluconazole users in this study represent a typical RVVC population, trends observed in microbial abundance require further analysis to establish fluconazole's long-term microbiome safety. Examining the microbiome at both sites adds to the current understanding of microbial associated with the condition.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"285-301"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Pediatric Respiratory Syncytial Virus (RSV) infections: has risk perception for RSV changed in children with comorbid conditions?","authors":"Dilek Yilmaz, Selin Tasar, Aysegul Elvan Tuz, Nesli Agralı Eroz, Eda Karadag Oncel, Ahu Kara Aksay, Nisel Yilmaz","doi":"10.1007/s10096-024-05003-6","DOIUrl":"10.1007/s10096-024-05003-6","url":null,"abstract":"<p><strong>Objective: </strong>Respiratory syncytial virus (RSV) is the primary etiology of lower respiratory tract infection in children. The fluctuating incidence of RSV, particularly in light of the COVID-19 pandemic, has shifted the spotlight onto preventive strategies. Our study aims to investigate both the risk factors and clinical symptoms of RSV.</p><p><strong>Materials and methods: </strong>From February 2015 to February 2023, samples were analyzed during all seasons to identify viral respiratory infections. RSV was identified in a total of 835 individuals.</p><p><strong>Results: </strong>In 2021, following the easing of limitations after the COVID-19 pandemic, the largest number of identified cases was recorded. January was the most commonly used month. The median age were 5 months (min-max: 1-204 months) and 128 (17.7%) cases had a history of prematurity. Around 24.7% of the patients had a preexisting medical condition. Neurological disease patients were followed up in the intensive care unit more often than others (53.3 vs. 35.8% p = 0.036). While the hospital stay of pediatric patients born under the 29th week of gestation is almost twice as long compared to other groups, the hospital stay is almost twice as long as that of patients between 29 and 32 weeks. (p = 0.046, p = 0.012 respectively).</p><p><strong>Conclusion: </strong>RSV was a powerful companion during the pandemic and a persistent reminder of its severity. Our initial data suggest that RSV prevention is difficult for children with pre-existing diseases, notably neurological abnormalities, who are not advised for preventive treatments. Given this outcome, late-premature newborns and children with medical issues should receive RSV prophylaxis first.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"333-342"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and prognostic insights into Chlamydia trachomatis in pediatric acute respiratory infections: evidence from targeted next-generation sequencing of 5,021 cases.","authors":"Chunyun Fu, Huan Zhang, Xiangjun Lu, Yanhua Feng, Lishai Mo, Junming Lu, Huiping Huang, Qiang Huang, Cuihong Lu, Wenting Tang, Ruting Chen, Silin Jiang, Ya Huang, Qifei Li, Jie Tan","doi":"10.1007/s10096-025-05049-0","DOIUrl":"https://doi.org/10.1007/s10096-025-05049-0","url":null,"abstract":"<p><strong>Background: </strong>Chlamydia trachomatis (CT) is a major cause of respiratory tract infections in children. The primary objective of this research was to evaluate the infection status and clinical manifestations associated with C. trachomatis in these pediatric patients.</p><p><strong>Methods: </strong>From April 2021 to November 2023, a total of 5,021 hospitalized children with acute respiratory tract infections were collected at the Guangxi Maternal and Child Health Hospital. tNGS was used to detect pathogens in their respiratory samples.</p><p><strong>Results: </strong>Among the 5,021 hospitalized pediatric patients with acute respiratory tract infections, targeted next-generation sequencing (tNGS) detected C. trachomatis in 138 cases, with a detection rate of 2.75%. Among the 138 cases positive for C. trachomatis, 10 were identified as single C. trachomatis infection, while 128 cases involved co-infections with other pathogens. A total of 36 additional pathogens were detected in children with mixed Chlamydia trachomatis infections, with Pneumocystis jirovecii being the most frequently detected. In children with C. trachomatis infection, pulmonary consolidation and hypoxemia were the most commonly observed respiratory complications, whereas anemia and liver function impairment were the primary complications affecting other organ systems. The median duration of hospitalization for the 138 children was 8 days. Among the 138 children with C. trachomatis infection, 108 cases (78.26%) required respiratory support, and 11 cases (7.97%) required ICU admission.</p><p><strong>Conclusion: </strong>The detection rate of C. trachomatis among hospitalized pediatric patients with respiratory tract infections was 2.75%. This study delineates the clinical manifestations and infection patterns of C. trachomatis in Guangxi, China.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of invasive Haemophilus influenzae infections in Belgium: 2018-2022.","authors":"Ricardo El Nouwar, Benoit Prevost, Magali Wautier, Nicolas Yin, Maya Hites, Delphine Martiny","doi":"10.1007/s10096-025-05040-9","DOIUrl":"https://doi.org/10.1007/s10096-025-05040-9","url":null,"abstract":"<p><strong>Introduction: </strong>Haemophilus influenzae plays a major role in invasive bacterial infections. Resistant strains are emerging, prompting the WHO to include H. influenzae on its list of priority pathogens for research and development of new antibiotics.</p><p><strong>Purpose: </strong>We aimed to describe the serotypes, demographics and susceptibility profiles of invasive strains collected in Belgium.</p><p><strong>Methods: </strong>Data on invasive strains referred to the Belgian National Reference Center for H. influenzae from 2018 to 2022 were analyzed retrospectively.</p><p><strong>Results: </strong>A total of 608 invasive strains were included. The number of notifications per year ranged from 85 to 165, with a marked decrease between 2020 and 2021. The highest incidence rate was observed in Brussels (1.56 per 100,000 inhabitants over the five year 2018-2022). Sex and age distribution were in line with global trends, as was the predominance of the non-typeable H. influenzae (NTHI). Beta-lactam resistance varies between molecules: 18.9% for ampicillin, 5.6% for amoxicillin-clavulanate. Mutations in the ftsI gene associated with decreased susceptibility to beta-lactams increased from 11.5 to 17.7% over the period studied.</p><p><strong>Discussion: </strong>The COVID-19 pandemic significantly influenced the epidemiology of invasive H. influenzae infections in Belgium. Demographic analysis identified a significant male predominance among infants, with a male-to-female ratio of 2.57 in patients under one year of age, a high figure not previously reported in the literature. The continued predominance of NTHI underscores the efficacy of Hib vaccination, although the emergence of Hib in patients younger than five years in 2022 suggests an alarming serotype dynamic. The detection of meropenem resistance also highlights the growing threat of antimicrobial resistance, while the increase in ftsI gene mutations raises concerns about the efficacy of first-line treatment.</p><p><strong>Conclusions: </strong>This study provides a comprehensive overview of the epidemiology of invasive H. influenzae infections in Belgium, focusing on demographic changes, serotype predominance and antimicrobial resistance trends. Vigilant surveillance and research are essential to address emerging challenges and guide future interventions, including potential vaccine development.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of reporting rapid susceptibility results in Gram negative bloodstream infections: a real world prospective study.","authors":"Anne-Sophie Messiaen, Stien Vandendriessche, Emilie De Muynck, Gregory Strubbe, Liesbet De Bus, Petra Schelstraete, Kristen Decommer, Sanne De Smet, Alexander Soetens, Leslie Naesens, Katrien Timmermans, Jan J De Waele, Diana Huis In 't Veld, Jerina Boelens","doi":"10.1007/s10096-025-05046-3","DOIUrl":"https://doi.org/10.1007/s10096-025-05046-3","url":null,"abstract":"<p><strong>Purpose: </strong>Mortality and morbidity of patients with bloodstream infection (BSI) remain high despite advances in diagnostic methods and efforts to speed up reporting. This study investigated the impact of reporting rapid Minimum Inhibitory Concentration (MIC)-results in Gram negative BSIs with the ASTar system (Q-linea, Uppsala, Sweden) on the adaptation of empirically started antimicrobial therapy. We performed a real-world study during which antimicrobial susceptibility testing (AST) results were instantly reported to the treating physician in an established multidisciplinary antimicrobial stewardship setting.</p><p><strong>Methods: </strong>Consecutive patients with Gram negative bacteremia were included in the study (monomicrobial Gram stain, life expectancy of at least 48 h and flagging positive before 2 PM). Rapid AST (RAST) reporting with ASTar was added on top of the standard workflow. Technical performance of the system was evaluated as well as the impact on antimicrobial treatment and timelines of achieving effective and optimal antimicrobial therapy.</p><p><strong>Results: </strong>A total of 79 analyses were performed in 77 patients, of which 68 episodes were eligible for analysis. A categorical agreement was observed in 97,5% of 1160 MIC results without false susceptible results. All patients on ineffective empirical therapy (12/68) were switched after a median time of approximately one hour (5 min - 15 h) after communication of the result. Furthermore, 20/55 non-optimal therapies were adapted within a median period of 3 h after communication.</p><p><strong>Conclusion: </strong>The implementation of rapid MICs, measured by the ASTar system, in our low antimicrobial resistance setting with elaborate antimicrobial guidelines, was easy and led to early adaptation of empirical treatment in 32/55 instances (12 ineffective and 20 non-optimal therapy).</p><p><strong>Trial registration number: </strong>NCT06218277 (date of registration: 18-12-2023).</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the AMP rapid test ROTA/ADENOVIRUS for simultaneous detection of rotavirus and adenovirus in stool samples.","authors":"Majd Allahham, Wolfgang Rennert, Kiija Manty Miller, Mustafa Barham, William Amoss, Musa Hindiyeh","doi":"10.1007/s10096-025-05042-7","DOIUrl":"https://doi.org/10.1007/s10096-025-05042-7","url":null,"abstract":"<p><strong>Background: </strong>Rotavirus (RV) and Adenovirus (AdV) gastroenteritis affect children worldwide. The sensitivity and specificity of the AMP Rapid Test ROTA/ADENOVIRUS [AMP-RA] for the detection of RV and AdV were compared against qPCR Allplex™ GI-Virus Assay [Allplex-GI].</p><p><strong>Methods: </strong>A cross-sectional hospital-based surveillance study was conducted using stool samples from 1,148 patients under the age of five years between July 2023 and May 2024 with gastroenteritis. All samples were tested using the AMP-RA and challenged with the Allplex-GI that detects rotavirus A, enteric adenovirus F, norovirus genogroups GI & GII, sapovirus, and astrovirus. RV samples testing positive by AMP-RA and/or Allplex-GI were subjected to genotyping.</p><p><strong>Results: </strong>Of the 1,148 stool samples, 123 samples tested positive for RV on AMP-RA while 133 tested positive on the Allplex™ GI. The clinical sensitivity and positive predictive value (PPV) of the AMP-RA for the detection of RV were 92.4% and 100%, respectively, while, the clinical specificity and negative predictive value (NPV) of the AMP-RA kit were 100% and 99%, respectively. The AMP-RA was able to detect all RV genotypes that circulated (G1[P8], G2[P4], G9[P8], G9[P4], G9[P9]). The AMP-RA assay detected 48 positive AdV samples and failed to detect 28 samples. Thus, the clinical sensitivity and PPV were 63.1% and 24.6%, respectively. 147 samples were positive for AdV on AMP-RA and negative by Allplex-GI, indicating a clinical specificity and NPV of 86.2% and 97.1%, respectively.</p><p><strong>Conclusion: </strong>AMP-RA showed high diagnostic sensitivity and specificity for RV detection. The reliability of detecting AdV was insufficient, emphasizing the need for further adenovirus test improvement.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}