European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Update on the epidemiology of dermatophytosis in Isfahan, Iran over the past decade.","authors":"Faezeh Rouhi, Mostafa Chadeganipour, Shahla Shadzi, AmirShayan Chadeganipour","doi":"10.1007/s10096-025-05286-3","DOIUrl":"https://doi.org/10.1007/s10096-025-05286-3","url":null,"abstract":"<p><strong>Background: </strong>Dermatophytes, keratinolytic fungi causing tinea (ringworm), are a major global cause of skin infections, affecting 20-25% of the population. This study updates the epidemiological profile of dermatophytosis in Isfahan, Iran, from 2013 to 2023, focusing on prevalence, and species distribution.</p><p><strong>Methods: </strong>This retrospective study analyzed 22,198 clinical samples from 2013 to 2023 at a reference medical mycology laboratory in Isfahan, Iran. Patient data, including demographics and infection sites, were recorded. Samples from skin, nails, and hair were examined using KOH direct microscopy and cultured on Sabouraud agar media with chloramphenicol and cycloheximide.</p><p><strong>Results: </strong>Over the decade, 22,198 clinical samples were analysed, of which 5,038 (22.7%) were confirmed by microscopy as dermatophytosis. Tinea corporis (22.79%) and tinea capitis (21.3%) were the most prevalent infections. Most cases occurred in females (58.57%) and individuals aged 41-50 years. High-risk groups included housewives, farmers, and livestock handlers. Among 770 cultured samples, Trichophyton mentagrophytes complex was the most common species.</p><p><strong>Conclusion: </strong>This study provides valuable insights into the epidemiology and clinical trends of dermatophytosis in Isfahan, Iran, over the past decade. Among the cultured cases, the T. mentagrophytes complex was the most frequently isolated species, suggesting a possible shift in the causative agents of tinea infections. However, since most confirmed cases were not cultured, the species distribution in the broader population remains uncertain. Further research using molecular identification and investigation of environmental factors will help improve our understanding and management of these infections.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of global carbapenemase-producing Serratia spp. (2015-2017).","authors":"Gisele Peirano, Yasufumi Matsumura, Johann D D Pitout","doi":"10.1007/s10096-025-05254-x","DOIUrl":"https://doi.org/10.1007/s10096-025-05254-x","url":null,"abstract":"<p><p>Limited information is available regarding the global molecular epidemiology of Serratia spp. with carbapenemases. We characterized carbapenemase-producing Serratia spp. (n = 56) using short read whole genome sequencing (WGS) obtained from global surveillance programs (2015-17). KPC (54%), VIM (16%) and NDM (14%) were the most common carbapenemases, followed by OXA-48 (10%), IMP-8 (four %), GES-5 (two %). The population was heterogenous, consisting of seven species and thirty-two clones. Serratia sarumanii (32%), Serratia nevei (23%), Serratia marcescens sensu stricto (20%) were the principal species. S. sarumanii contained three dominant clones: ST795 with bla_VIM-1 from Italy; ST256 with bla_KPC-3 from USA; ST428 with bla_KPC-2 from Colombia and China. S. nevei contained two dominant clones: ST893 with various carbapenemases from South Africa; ST325 with bla_KPC-2 from Colombia, and with bla_IMP-8 from Taiwan. Class I integrons (In916 with bla_VIM-1, In238 with bla_VIM-4 and In73 with bla_IMP-8) circulated between various Enterobacterales species and clones in Europe and Taiwan. Carbapenemase-producing Serratia spp. disseminated either clonally and polyclonally, depending on the species, clones and geographical location. Whole genome sequencing offered unprecedented details about global carbapenemase-producing Enterobacterales species obtained from the same surveillance programs over similar time periods. This work also highlights the need to address the taxonomy of Serratia spp. since confusion currently exists regarding species and sub-species within this genus.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized detection of ESBL producers, carbapenemase producers, and VRE from rectal swabs using enrichment broth for superior screening sensitivity.","authors":"Dominique S Blanc, Massiha Kamyar, Patrice Nordmann, Laurent Poirel, Gilbert Greub","doi":"10.1007/s10096-025-05288-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05288-1","url":null,"abstract":"<p><p>This study evaluates the added value of using enrichment broths for improving the detection of multidrug-resistant (MDR) bacteria-VRE, ESBL producers, and carbapenemase-producing organisms (CPOs)-from rectal swabs. Comparing direct plating to enrichment-based methods significantly increased detection sensitivity, especially for CPOs (from 17 to 27 cases, a 58.8% increase). Detection rates for ESBL producers and VRE also improved significantly (by 20%). Pre-enrichment is therefore a valuable addition to routine MDR bacteria screening protocols, which is particularly useful when high accuracy is needed for prevention and infection control as well as for outbreak management.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weissella confusa aortic valve endocarditis: A case report and literature review.","authors":"Jules Massart, Julien Bador, Jean Christophe Eicher, Gabriel Laurent, Georges Tarris, Marielle Buisson, Romain Didier, Arnaud Magallon","doi":"10.1007/s10096-025-05277-4","DOIUrl":"https://doi.org/10.1007/s10096-025-05277-4","url":null,"abstract":"<p><p>Weissella are Gram-positive coccobacilli found in fermented foods and considered to have probiotic potential. However, different species of this bacterial genus may be involved in human infections, particularly in cases of immunodepression. We report a rare case of Weissella confusa endocarditis in a 65-year-old man with myelodysplastic syndrome, combined with a review of other cases described in the literature. As identification to species level was challenging with the standard techniques, we resorted to whole genome sequence analysis.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus aureus genotypic penicillin susceptibility using a blaZ qPCR directly on blood cultures shows high concordance with phenotype.","authors":"Tobias Müller Vang, Barbara Juliane Holzknecht, Stephen Strunge Nilsson, Morten Hoppe, Michael Pedersen, Karen Angeliki Krogfelt, Helle Brander Eriksen","doi":"10.1007/s10096-025-05272-9","DOIUrl":"https://doi.org/10.1007/s10096-025-05272-9","url":null,"abstract":"<p><p>Staphylococcus aureus blood cultures were investigated geno- and phenotypically for penicillin susceptibility. A qPCR method detecting the blaZ gene was performed directly on positive blood culture vials and compared with routine disk diffusion results supplemented with the cloverleaf test. Among the collected vials, 100 were blaZ positive and 101 were blaZ negative. Full concordance was observed between genotypical and phenotypical penicillin susceptibility, however one penicillin resistant isolate was only weakly phenotypically positive.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic characteristics of the Rapid ESBL NP^® test and ResistanceFinder 2SMART multiplex PCR for the detection of ESBL directly on urine samples.","authors":"Marit W Bot, Jacky Flipse, Sabien L M Bongers, Chiara Giardino, Lieke E K W Geraets, Antoinette A T P Brink, Jacobien J Hoogerwerf, Jaap Ten Oever, Heiman F L Wertheim, Maurits P A van Meer","doi":"10.1007/s10096-025-05256-9","DOIUrl":"https://doi.org/10.1007/s10096-025-05256-9","url":null,"abstract":"","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of three-dimensional bacteriophage cocktail biogel on infected burn wounds in rats.","authors":"Elif Aydin, A Kocak Sezgin, M Koldemir Gunduz, G Kaymak, B Açikgoz, F Aslan, S Ali, A Karaynir, B Bozdoğan","doi":"10.1007/s10096-025-05269-4","DOIUrl":"https://doi.org/10.1007/s10096-025-05269-4","url":null,"abstract":"<p><p>Burn wound infections significantly hinder the healing process by disrupting the immune response and limiting treatment options due to increasing antibiotic resistance. In this study, the antimicrobial and tissue regeneration effects of a three-dimensional bacteriophage cocktail bio-gel were evaluated in vivo in burn wounds infected with antibiotic-resistant Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae. Using an experimental burn model in Sprague Dawley rats, the effects of phage bio-gel treatment on inflammatory response, cellular repair mechanisms, and wound closure dynamics were analyzed through molecular, biochemical, and histopathological assessments. Application of the bacteriophage bio-gel significantly accelerated wound healing in infected burn groups, with healing rates ranging from 77.56 to 89.75% on days 15 and 20. Cytokine analysis demonstrated that phage therapy modulated inflammation by reducing IL-1β, IL-6, and TNF-α levels (p < 0.05). Molecular analyses examining extracellular matrix dynamics showed a significant increase in TGF-β1, Smad-2/3, and collagen type 1 gene expression, indicating enhanced fibroblast activation and tissue remodeling. While MMP-2 and hydroxyproline levels increased, MMP-9 levels decreased following phage treatment. Histopathological evaluations revealed that re-epithelialization and tissue remodeling were accelerated, while inflammation was reduced in the A. baumannii and K. pneumoniae infected groups. These findings suggest that bacteriophage-based bio-gels provide a promising alternative for treating polymicrobial burn wound infections. The results indicate that bacteriophage biomaterials exhibit superior wound healing potential compared to conventional treatments, particularly in infections caused by antibiotic-resistant pathogens.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of the gut-lung axis in patients with viral pneumonia and respiratory failure.","authors":"Yirun Li, Bang Yu, Chun Wang, Qinqi Feng, Jian Liu, Hao Zou, Hongchun Zhang, Xiaohui Zou","doi":"10.1007/s10096-025-05271-w","DOIUrl":"https://doi.org/10.1007/s10096-025-05271-w","url":null,"abstract":"<p><p>Respiratory viral infections can influence the human microbiota, promoting disease progression and complications. The gut-lung axis (GLA), as a critical pathway for microecological regulation and immune modulation, remains poorly understood in terms of its microbial characteristics and role in respiratory failure. The aim of this study was to elucidate the microbial characteristics and functions of the upper respiratory tract and gut and their associations in patients with viral pneumonia complicated by respiratory failure. We included three groups of individuals: healthy controls (HT), viral pneumonia patients without respiratory failure (NRF), and those with respiratory failure (RF), and systematically analyzed the characteristics and associations of the upper respiratory tract and gut microbiota. Beta diversity analysis revealed significant separation of upper respiratory tract and gut microbial communities among the three groups, while alpha diversity decreased with increasing disease severity. LEfSe analysis and correlation analyses targeting complete blood count parameters and serum inflammatory factors revealed synchronous enrichment of Rothia in the upper respiratory tract and Enterococcus in the gut of the RF group. Prevotella was enriched in the gut of the HT group but abnormally increased in the upper respiratory tract of the RF group, reflecting ecological niche shifts. Functional prediction indicated significant enrichment of pathways such as beta-lactam resistance in the RF group. Several genera (such as Blautia and Prevotella) were highly correlated with inflammatory cytokines (IL-1β, IL-6, CRP) and platelet counts, suggesting a microbiota-inflammation-immunity interplay. This study reveals significant dysbiosis of the gut-lung axis in patients with viral pneumonia and respiratory failure, with the Rothia-Enterococcus axis serving as a potential early warning and intervention target. The dynamic changes of the gut-lung microbiota and its association with inflammation and immunity provide a theoretical basis for microbiota-based therapies.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Emergence of a CTX-M-15-producing multidrug-resistance Haemophilus parainfluenzae in Southern Spain\".","authors":"María Pérez-Vázquez, Virginia Pérez-Carrasco, María Aguilera-Franco, Noelia Lara, Mar Rivero-Rodríguez, José María Navarro-Marí, José Gutiérrez-Fernández","doi":"10.1007/s10096-025-05287-2","DOIUrl":"https://doi.org/10.1007/s10096-025-05287-2","url":null,"abstract":"<p><p>Urogenital infections caused by Haemophilus parainfluenzae are becoming an emerging health problem. This, along with the increase in strains resistant to current antibiotic treatments, is making it difficult to control these infections. Therefore, it is important to monitor the emergence and spread of these strains, as well as their resistance to antibiotics. Our study describes a new urethral isolate of MDR with ESBLs from a hospital in southern Spain. Whole-genome sequencing has determined the production of extended-spectrum beta-lactamase (CTX-M), as well as the presence of other resistance genes. Furthermore, phylogenetic analysis with respect to other isolates has shown the presence of allelic differences between them, justifying the geographical spread of this clinical problem.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcus pneumoniae bacteremia: comparison of incidence, epidemiology, and clinical outcome in a pre- and post-COVID-19 period.","authors":"Andrea Bedini, Maria Daria Di Trapani, Giacomo Franceschi, Stefano Zona, Marianna Meschiari, Alessandra Soffritti, Mattia Simion, Erica Franceschini, Caterina Vocale, Tiziana Lazzarotto, Mario Sarti, Cristina Mussini","doi":"10.1007/s10096-025-05267-6","DOIUrl":"https://doi.org/10.1007/s10096-025-05267-6","url":null,"abstract":"<p><strong>Objectives: </strong>Streptococcus pneumoniae is a leading cause of severe bacterial infections, including pneumonia, meningitis, and bacteremia. We aimed to evaluate the differences in clinical, epidemiological and outcome manifestations of patients admitted with S. pneumoniae bacteremia (SPB) in the pre- and post-COVID-19 period.</p><p><strong>Methods: </strong>The study analyzed all patients admitted in Province of Modena, Italy, during two time-periods: pre-COVID-19 (Jan 2018- Feb 2020) and post-COVID-19 (Mar 2020- Jun 2022) period. The data were compared using univariate and multivariate analysis.</p><p><strong>Results: </strong>A total of 150 patients with SPB were included, 109 and 41 patients in the pre- and post-COVID-19, respectively. We observed a decrease in SPB incidence from March 2020, after the implementation of the restrictive measures for the COVID-19 pandemic, and a new increase since Jun 2021, when lockdown measures were fully lifted. SPB was associated with pneumonia in 128 patients (85.3%), meningitis in 25 (16.7%) and otitis-mastoiditis in 14 (9.3%). The proportion of patients presenting with multi-lobar pneumonia significantly increased during the post-COVID-19 period (39.0% vs. 16.5%, p = 0.008). Thirty-day mortality rate resulted higher in the post-COVID-19 period (24.4% vs. 11.9%, p = 0.075), and multivariate analysis identified an age ≥ 80 years (OR 4.45, 95% CI 1.12-17.61, p = 0.033), multi-lobar pneumonia (OR 4.34, 95% CI 1.56-12.07, p = 0.005), and central nervous system disease (OR 3.63, 95% CI 1.08-12.20, p = 0.036) as independent risk factors for 30-day mortality. The rate of pneumococcal vaccination in the at-risk population was low (9.3%), but in the pandemic period the rate increased by 26.7%, driven by the anti-SARS-CoV-2 vaccination campaign.</p><p><strong>Conclusions: </strong>The COVID-19 pandemic has impacted the epidemiology and clinical severity of SPB. In our study, less than 10% of the high-risk population was vaccinated, while the older population (age ≥ 80 years) had a significantly higher 30-day mortality risk. It would be necessary for Institutions to increase awareness campaigns for pneumococcal vaccination.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}