European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Fracture related infection complicating civilian ballistic wounds in the amasonian zone.","authors":"Karamba Sylla, Xinggang Lu, Adrien Mons, Vincent Sainterose, Paul Le Turnier, Loic Epelboin, Felix Djossou, Arsène A Kpangon, Philippe Abboud, Fouzia Sariak, Richard Naldjinan, Renaud Scussel, Morgane Bourne, Fredegonde About, Olivier Lesens","doi":"10.1007/s10096-025-05203-8","DOIUrl":"10.1007/s10096-025-05203-8","url":null,"abstract":"<p><strong>Introduction: </strong>Fracture-Related Infections (FRI) are one of the most common complications of ballistic wounds sustained in military operations. There is very little data on ballistic wounds in civilians, especially FRIs. There is no data at all on FRIs in French Guiana, which has one of the highest rates of delinquency and gun ownership in France.</p><p><strong>Patients and methods: </strong>We conducted a retrospective study, including patients admitted to Cayenne Hospital with civilian ballistic wounds. Patients with incomplete records were excluded. The following data were collected: wound location, infectious complications and microbiological documentation. Chi-squared tests were used for categorical variables, a Student's t-tests for continuous variables and a logistic regression for multivariate analysis.</p><p><strong>Results: </strong>Of the 196 patients included in the study, the overall reported infection rate was 14%. FRIs were the most frequent infectious complication, occurring in 15% (14 out of 95 fractures caused by ballistic wounds). Of these, 53 were open fractures, 27 of which were classified as Gustilo ≥ 2. Six (43%) of the 14 FRIs were monomicrobial, including four methicillin-susceptible S. aureus. Eight (57%) were polymicrobial, seven of which had at least one Gram-negative bacillus in culture. Sixteen Gram-negative bacilli, including ten group 3 Enterobacteriaceae, were isolated from eight patients. The presence of an external fixator (adjusted odds ratio (aOR) [95% confidence interval (CI)]: 28.9 [5.9-139.9], p < 0.001) and comorbidity (diabetes, steroids or HIV) (aOR [95% CI]: 37.5 [4.5-308.4], p = 0.001) were significantly associated with FRI occurrence in the multivariate analysis.</p><p><strong>Conclusion: </strong>FRIs are one of the most common complications from civilian ballistic wounds in French Guiana. FRIs are often polymicrobial, with a high prevalence of Gram-negative bacteria. FRIs are favored by comorbidities and the presence of an external fixator.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2401-2408"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential miRNA signatures in Hepatitis E Virus Infection: Insights into acute, chronic, and pregnancy-related outcomes.","authors":"Nancy León-Janampa, Julien Marlet","doi":"10.1007/s10096-025-05207-4","DOIUrl":"10.1007/s10096-025-05207-4","url":null,"abstract":"<p><strong>Purpose: </strong>Hepatitis E virus (HEV) is a positive single-stranded RNA virus that causes acute and chronic hepatitis with severe complications in pregnant women and immunocompromised individuals. HEV infection manifest asymptomatically or progress to fulminant hepatitis, liver failure, and extrahepatic manifestations such as neurological disorders and renal damage. MicroRNAs are non-coding RNA molecules that play a key role in diseases and viral pathogenesis, influencing viral replication and immune evasion through their interaction with host factors. In this review, we discuss the role of microRNAs described to date in hepatitis E infection, highlighting their impact on acute, chronic, and pregnancy-related clinical outcomes.</p><p><strong>Results: </strong>miR-122, miR-214, miR-221, and miR-222 participate in the HEV replication and immune evasion, while specific miRNA profiles distinguish acute and chronic hepatitis E. HEV poses severe risks in pregnancy, with miR-431, miR-654, and miR-1468 related to self-limiting infection, and miR-450b to acute liver failure. Studying these miRNAs lead to biomarkers and therapies for hepatitis E.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2303-2321"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterium shigaense infection: a case and brief literature review.","authors":"Feifei Zhao, Yu Feng, Dan Zhou, Yi Xie, Zhiyong Zong","doi":"10.1007/s10096-025-05296-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05296-1","url":null,"abstract":"<p><p>Infections due to Mycobacterium shigaense, a slow-growing mycobacterium of the Mycobacterium simiae complex, are rarely reported. We describe a case of M. shigaense infection in a 68-year-old woman with diabetes and found that its infections are often associated with immunocompromised conditions or underlying pulmonary diseases in East Asia.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of EUCAST rapid antimicrobial susceptibility testing (RAST) for carbapenemase-producing Klebsiella pneumoniae with focus on KPC variants.","authors":"Min Xu, Yuchao Zhang, Yajie Fu, Qiaoling Sun, Haishen Kong, Yiqi Fu","doi":"10.1007/s10096-025-05290-7","DOIUrl":"https://doi.org/10.1007/s10096-025-05290-7","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the efficacy of the EUCAST Rapid Antimicrobial Susceptibility Testing (RAST) on Klebsiella pneumoniae carrying various carbapenemases, with a focus on KPC variants.</p><p><strong>Methods: </strong>A total of 130 carbapenemase-producing K. pneumoniae (CPKP), encompassing those harboring class A (KPC-2: n = 38; KPC variants: n = 30), class B (n = 42), class D (n = 4) and strains co-producing class A and class B carbapenemases (n = 16), were evaluated for susceptibility to seven antibiotics, including ceftazidime/avibactam (CAZ/AVI), using the EUCAST RAST. Results obtained after 6 h and 8 h of incubation were compared with those obtained by reference broth microdilution. Additionally, the capacity of the RAST method to screen for different types of carbapenemases was assessed.</p><p><strong>Results: </strong>All 130 CPKP isolates generated 100% readable zones at both 6 h and 8 h, with overall categorical agreement (CA) rates of > 90% for all tested antibiotics. For isolates producing class A carbapenemases, particularly the KPC variants, the EUCAST RAST showed excellent performance in determining CAZ/AVI susceptibility, achieving 100% CA and no errors at each reading time. However, significant challenges arose for meropenem (MEM), characterized by low CA (73.9%) and high major errors (MEs, 20.1%) at 6 h reading. Extending incubation to 8 h dramatically improved the performance, while the proportion of strains within the ATU remained high (23.3% at 6 h; 30.0% at 8 h).</p><p><strong>Conclusions: </strong>The EUCAST RAST can be effectively implemented in routine clinical laboratories, particularly in regions like China where K. pneumoniae carrying KPC-2 is widely prevalent. However, caution should be exercised when interpreting the results of MEM for KPC variants.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of Klebsiella pneumoniae causing bloodstream infections in a district hospital in Northern Portugal (2016-2018): clonal diversity and detection of hypervirulent strains.","authors":"Michele Loiodice, Tatiana Ribeiro, Luís Marques Silva, Ana Paula Castro, Luísa Peixe, Ângela Novais","doi":"10.1007/s10096-025-05260-z","DOIUrl":"https://doi.org/10.1007/s10096-025-05260-z","url":null,"abstract":"<p><strong>Purpose: </strong>Klebsiella pneumoniae (Kp) is a major cause of bloodstream infections (BSI) globally. Yet, detailed characterization of invasive isolates, particularly from datasets not biased toward resistance, remains limited. This baseline study, conducted prior to the expansion of carbapenemase producers in Portugal, characterizes multidrug-resistant (MDR) and non-MDR Kp causing BSIs in a northern district hospital to elucidate resistance emergence and dissemination.</p><p><strong>Methods: </strong>A total of 174 Kp strains isolated from blood cultures of hospitalized patients (2016-2018) were identified by VITEK2 or MALDI-TOF MS. Antibiotic resistance phenotypes were determined by reference methods, β-lactamases screened by PCR/sequencing, and clonal sublineages identified by FT-IR spectroscopy, MLST and/or WGS, including for putative hypervirulent strains (hvKp).</p><p><strong>Results: </strong>Isolates were categorized as MDR (49%) or non-MDR (51%), with MDR rates nearly double national reports. Most MDR-Kp (67%) produced CTX-M-15, 25% produced carbapenemases (mainly KPC-3 and/or OXA-48/CTX-M-15), and 8% lacked acquired β-lactamases. Half (53%) belonged to three high-risk sublineages (ST147-KL64, ST307-KL102, ST15-KL19), while non-MDR showed greater diversity, with ST14-KL2 most frequent (13%) and consistently resistant to amoxicillin-clavulanate. Several sublineages circulated across both cohorts (e.g., ST307-KL102, ST348-KL62), some with variable β-lactamase content (e.g., ST147-KL64, ST45-KL24). Two hvKp (1.1%) were identified (ST380-KL2, ST268-KL20).</p><p><strong>Conclusion: </strong>This baseline study reveals a high local burden of MDR-Kp driven by CTX-M-15 and KPC-3-producing high-risk sublineages, underscoring the importance of local surveillance beyond national reporting. High clonal diversity and variable β-lactamase content suggest heterogeneous acquisition and dissemination routes, with resistance often preceding clonal expansion. Though rare, hvKp detection underscores the need for continued surveillance and multicenter monitoring.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic identification and characterization of Streptococcus oralis group that causes intraamniotic infection.","authors":"Pisut Pongchaikul, Puntabut Warintaksa, Thidathip Wongsurawat, Piroon Jenjaroenpun, Worarat Kruasuwan, Paninee Mongkolsuk, Pornpun Vivithanaporn, Iyarit Thaipisuttikul, Arunee Singhsnaeh, Jakkrit Khamphakul, Suwatcharaporn Hadradchai, Maolee Bhuwapathanapun, Piya Chaemsaithong","doi":"10.1007/s10096-025-05283-6","DOIUrl":"https://doi.org/10.1007/s10096-025-05283-6","url":null,"abstract":"<p><strong>Background: </strong>Intraamniotic infection is a cause of spontaneous preterm labor. Streptococcus mitis is a common pathogen identified in intraamniotic infection, with the possible route of hematogenous dissemination from the oral cavity or migration from the vaginal canal. However, there are a few reports on Streptococcus oralis, a member of the S. mitis group, as a cause of pathogen in intraamniotic infection. We reported herein whole genome sequencing and comparative genomic analysis of S. oralis strain RAOG5826 that causes intraamniotic infection.</p><p><strong>Results: </strong>Streptococcus mitis was initially identified from amniotic fluid, vaginal swab, and fetal blood of a patient presenting with preterm prelabor rupture of membranes with intraamniotic infection by the use of conventional microbiological methods (biochemical phenotype, MALDI-ToF, 16 S rRNA). Subsequently, this strain was later identified as S. oralis RAOG5826 by whole-genome hybrid sequencing. Genes involved in macrolide and tetracycline resistance, namely ermB and tet(M), and mutations in penicillin-binding protein were present in the genome. Moreover, potential virulence genes were predicted and compared with other Streptococcal species.</p><p><strong>Conclusion: </strong>We reported a comprehensive genomic analysis of S. oralis, which causes intraamniotic infection. S. mitis was initially identified by conventional microbiological identification. However, whole-genome hybrid sequencing demonstrates S. oralis with complete profiles of antimicrobial resistance genes and potential virulence factors. This study highlights the limitations of traditional techniques and underscores the importance of genomic sequencing for accurate diagnosis and tailored antimicrobial treatment. The study also suggests that S. oralis may be an underestimated pathogen in intraamniotic infection.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterobacter cloacae complex taxonomic incongruence may lead to misidentification of outbreaks and hinder infection control in real time.","authors":"Ella Kaplan, Mor N Lurie-Weinberger, Ofir Kastel, Adi Cohen, David Schwartz, Alona Keren-Paz, Yehuda Carmeli","doi":"10.1007/s10096-025-05282-7","DOIUrl":"https://doi.org/10.1007/s10096-025-05282-7","url":null,"abstract":"<p><p>The Enterobacter cloacae complex (ECC) are important nosocomial pathogens involved in hospital outbreaks. They present a significant challenge for accurate species identification, hindering outbreak detection and investigation. Nevertheless, many clinical laboratories report identification of these organisms at the species level. Here we report on several outbreaks of which recognition was delayed due to ECC erroneous species identification by common laboratory methods. We evaluated different classification approaches, including Vitek-2, mass spectrometry, 16 S rRNA sequencing, Fourier-transform infrared (FT-IR) spectroscopy and genomic-based methods. We collected 63 ECC isolates reported in a single hospital over 18 months and chose 17 isolates that were temporary close but recognized as different species by MALDI-TOF MS, and thus not suspected of being related. Whole genome sequencing followed by Average Nucleotide Identity (ANI) analysis revealed that those isolates represented several outbreaks. Using ANI analysis as the gold standard for ECC species identification, core-genome MLST (cgMLST) was the most accurate method, followed by FT-IR typing. We therefore suggest that clinical laboratories should report ECC isolates as such, without specifying the species. Alternatively, infection control teams need to be informed that all ECC isolates should be regarded as potentially related, regardless of their specific species designation.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectious spondylodiscitis: Epidemiology, diagnosis, microbiological findings, clinical features and outcomes in a 14-year retrospective study.","authors":"Jacopo Conti, Nicholas Geremia, Stefano Di Bella, Fulvio Zadra, Verena Zerbato, Stella Babich, Venera Costantino, Manuela Di Santolo, Marina Busetti, Filippo Mearelli, Leonello Tacconi, Marco Francesco Maria Cavallaro, Maria Assunta Cova, Gianni Biolo, Roberto Luzzati, Donatella Giacomazzi","doi":"10.1007/s10096-025-05292-5","DOIUrl":"https://doi.org/10.1007/s10096-025-05292-5","url":null,"abstract":"<p><strong>Purpose: </strong>To describe the epidemiology, diagnosis, microbiological findings, clinical features, and outcomes of infectious spondylodiscitis (IS).</p><p><strong>Methods: </strong>Retrospective analysis of 98 IS patients (2010-2023 - Trieste Hospital). Clinical, radiological, and microbiological data analysed, multivariate logistic regression assessed risk factors for poor outcomes.</p><p><strong>Results: </strong>The incidence of IS was 3 cases per 100,000 inhabitants per year. Pyogenic infections accounted for 54% of cases, tuberculosis for 10%, while 35% remained of unknown etiology. Back pain (79%) and fever (72%) were the most common symptoms. Staphylococcus aureus was the most common pathogen, with 34 cases, representing 64% of pyogenic spondylodiscitis. Blood cultures were positive in 47% of pyogenic cases, 25% of all cases. PET/CT showed higher diagnostic utility (83%) than labeled leukocyte scintigraphy (54%). The lumbar spine was the most affected region (63%), followed by thoracic (25%) and cervical (12%). Neurosurgical biopsy showed a 33.3% positivity rate, while CT-guided biopsy yielded 22%. Cervical (OR 4.76) and thoracic (OR 3.89) involvement were associated with worse outcomes. Major complications included radicular nerve damage (51%), epidural/paravertebral abscesses (45%), endocarditis (14%), and a need for surgical intervention in 8%, with persistent neurological sequelae in 3%. Infection-related mortality rate was 6%.</p><p><strong>Conclusion: </strong>S. aureus remains the leading pathogen in IS. Blood cultures play a key role, yielding positive in half of pyogenic cases. PET/CT surpassed leukocyte scintigraphy and improved MRI diagnostic accuracy. Lumbar involvement correlates with better outcomes, and open biopsy provides a higher diagnostic yield than percutaneous TC-guided biopsy. Empirical therapy is required in one-third of cases.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of contezolid in the treatment of Gram-positive bacterial infections in patients with hematological malignancies: a retrospective study.","authors":"Ruihua Mi, Luyang Chang, Lin Chen, Lin Wang, Yixuan Ma, Jia Liu, Dongbei Li, Xudong Wei","doi":"10.1007/s10096-025-05280-9","DOIUrl":"https://doi.org/10.1007/s10096-025-05280-9","url":null,"abstract":"<p><strong>Purpose: </strong>Contezolid, an oxazolidinone antibiotic, is recognized for its comparable antimicrobial activity to linezolid, with a distinct advantage of reduced hematological toxicity. This retrospective analysis aims to evaluate the safety and efficacy profile of contezolid in the treatment of Gram-positive bacterial infections (GPI) in hematological patients.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on adult hematological patients with confirmed or suspected GPI who received contezolid for at least three days following chemotherapy or hematopoietic stem cell transplantation (HSCT) at Henan Cancer Hospital between April 2022 and June 2024. Comprehensive data collection included demographic variables, treatment protocols, infection profiles, microbiological data, and clinical outcomes.</p><p><strong>Results: </strong>The study included 132 patients (47.5 ± 18.0 years), 61.4% male. Treatment strategies for hematological malignancies included combination chemotherapy with targeted therapy (41.7%), chemotherapy alone (37.9%), and HSCT (20.5%). 60.6% had febrile neutropenia. More than half of the infections occurred within one month after treatment, predominantly affecting the lungs and skin/soft tissue. Two patients had concurrent bloodstream infections (BSI) prior to receiving contezolid treatment. The median contezolid treatment was 12 days, 72.7% had it as initial treatment. The rationale for contezolid utilization includes complicated skin and soft-tissue infections (cSSTI), prior treatment failure, adverse reactions, and contraindications. Clinical effective rate was 70.6% (95% CI: 62.7% - 78.5%), with 64.4% (95% CI: 52.2% - 76.6%) in pneumonia, 74.6% (95% CI: 63.0% - 86.1%) in skin or soft tissue infections, and 69.3% (95% CI: 58.9% - 79.8%) in febrile neutropenia. Seventeen patients (12.9%) had adverse events. Twelve had new-onset/aggravated nausea or epigastric discomfort, and seven had vomiting; these may relate to contezolid. No contezolid-related hematological adverse events, optic or peripheral neuropathy were observed.</p><p><strong>Conclusions: </strong>Contezolid seems promising for these patients, but large-scale prospective studies are needed for confirmation.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of colistin resistance from Escherichia colicolonies grown on agar cultures and bacterial pellets obtained from positively flagged blood cultures by MALDI-TOF MS-based rapid assay: aproof-of-concept study.","authors":"Ekin Kırbaş, Ilka D Nix, Katrin Sparbier, Boris Oberheitmann, Markus Kostrzewa, Banu Sancak","doi":"10.1007/s10096-025-05268-5","DOIUrl":"https://doi.org/10.1007/s10096-025-05268-5","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;Polymyxins (polymyxin B and colistin) are one of the last-resort antibiotics, whose use is limited in the alternative treatment of certain infections due to carbapenem-resistant Enterobacterales, difficult-to-treat Pseudomonas aeruginosa, and carbapenem-resistant Acinetobacter baumannii. With the increased usage of colistin in clinical settings, the colistin resistance among Enterobacterales gradually increased and became one of the most important public health threats. Therefore, the rapid and accurate detection of colistin resistance have a significant effect on the mortality rate of Gram-negative bacterial infections. For this purpose, new methodologies are being developed, and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS)-based tools are one of these promising techniques.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 103 Escherichia coli isolates were included in the study (57 colistin-susceptible and 46 colistin-resistant). For the determination of colistin resistance, strains were tested with the MALDI Biotyper sirius System (Research Use Only [RUO]; Bruker Daltonics GmbH & Co. KG, Germany) equipped with the MBT HT LipidART Module (RUO; Bruker Daltonics GmbH & Co. KG, Germany) using MALDI-TOF MS in negative-ion mode. In these processes, two starting materials were used: (I) Bacteria grown on agar culture (AC), (II) bacterial pellets obtained from positively flagged blood cultures (BC) purified by the MALDI Sepsityper Kit 50 (Bruker Daltonics GmbH & Co. KG, Germany). The research use only MBT Lipid Xtract™ Kit (RUO; Bruker Daltonics GmbH & Co. KG; Germany) was used for the extraction of Lipid A molecules from bacteria grown on AC and from bacterial pellets of BCs. The performance of the MBT HT LipidART Module was determined for both sample types. The colistin susceptibility was tested by a broth microdilution based commercial kit (UMIC Colistin; Bruker Daltonics GmbH & Co. KG, Germany), as the reference method.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Using the MBT HT LipidART Module in Compass HT, the sensitivity and specificity of the AC-derived samples were determined to be 97.7%, and 98.2%, respectively. For BC-derived samples, the corresponding values were 93.3%, and 94.7%, respectively. The AC-grown E. coli strains (both colistin-susceptible and colistin-resistant), and the BC-grown, colistin-susceptible E. coli strains had a dominating, native Lipid A peak at m/z 1796. In contrast, in BC-grown, colistin-resistant E. coli isolates, three dominant native Lipid A peaks (m/z 1796, m/z 1824, and m/z 1840) were observed. In addition, modified Lipid A peaks at m/z 1919 and m/z 2043 were observed in the mass spectra.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The MBT Lipid Xtract™ Kit (RUO) in combination with the MBT HT LipidART Module (RUO) proves to be a promising tool for an effective detection of colistin resistance from AC-grown bacterial isolates and BC-derived samples, after app","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}