European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Community-acquired bacterial meningitis in Southern Sweden 2013-2023: a population-based study of incidence, aetiology and diagnostic yield.","authors":"Tobias West, Robin Carlander, Torgny Sunnerhagen, Gustav Torisson, Oskar Ljungquist","doi":"10.1007/s10096-025-05247-w","DOIUrl":"https://doi.org/10.1007/s10096-025-05247-w","url":null,"abstract":"<p><strong>Purpose: </strong>Despite advances in medical care, bacterial meningitis still poses a considerable health issue from a global perspective. An ageing population and increasing development and use of vaccines are likely to affect the incidence and aetiology. The aim of this study was to describe the incidence and aetiology of community-acquired bacterial meningitis (CABM) in our setting, as well as the serotypes of Streptococcus pneumoniae and Neisseria meningitidis causing CABM, in relation to available vaccines.</p><p><strong>Method: </strong>Patients of all ages with CABM in southern Sweden 2013-2023 were included. Patients were identified through records of cerebrospinal fluid tests from the Department of Clinical Microbiology, and through International Classification of Diseases 10 codes for bacterial meningitis. Age-standardised incidence rates were calculated based on the European Standard Population 2013.</p><p><strong>Results: </strong>During the study period, 244 episodes of CABM in 238 individuals were identified. Definitive aetiology could be established in 93% of episodes. Mean incidence rate was 1.63 per 100,000 person-years, with a trend of declining incidence during the study period. Streptococcus pneumoniae was the most common pathogen, accounting for 46.7% of episodes. 63.6% of the episodes of pneumococcal meningitis were caused by serotypes included in current vaccines.</p><p><strong>Conclusion: </strong>Pneumococcal meningitis is the primary driver of incidence and trends of all-cause community-acquired bacterial meningitis in southern Sweden. Further studies are warranted to investigate how vaccination could influence both disease incidence and shifts in serotype distribution, while also identifying optimal patient populations for targeted interventions.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal microbiota transplant as treatment for recurrent urinary tract infections: a proof-of-concept study.","authors":"Verónica Rico-Caballero, Mario Romero-Rivera, Ana Moreno-Blanco, Andrea Aira, Climent Casals-Pascual, Concepción Rodríguez-Jiménez, Carmen Quereda, Alex Soriano, Rosa Del Campo","doi":"10.1007/s10096-025-05202-9","DOIUrl":"10.1007/s10096-025-05202-9","url":null,"abstract":"","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2549-2554"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, microbiological, and prognostic differences between hypermucoviscous and non-hypermucoviscous community-acquired Klebsiella pneumoniae infections.","authors":"Hongkui Sun, Miaolian Chen, Xiaoxing Huang","doi":"10.1007/s10096-025-05197-3","DOIUrl":"10.1007/s10096-025-05197-3","url":null,"abstract":"<p><strong>Objective: </strong>This study investigates the clinical, microbiological, and prognostic variations between hypermucoviscous Klebsiella pneumoniae (hmKp) and non-hypermucoviscous Kp (n-hmKp) infections.</p><p><strong>Methods: </strong>A total of 381 cases of community-acquired Klebsiella pneumoniae (cKp) infections at Zhongshan People's Hospital between January 2019 and November 2024 were analyzed. Infection characteristics, mortality risk factors, and antimicrobial susceptibility profiles were compared between hmKp and n-hmKp groups.</p><p><strong>Results: </strong>No significant differences were observed between patients infected with n-hmKp and hmKp in terms of median age, gender, underlying conditions, hospital stay, ICU stay, and hospital mortality rates (all P > 0.05). However, the SOFA score was higher, and shock occurred more frequently in hmKp infections (all P < 0.05). In addition, hmKp exhibits a higher propensity for inducing invasive infections (P = 0.000). Multivariable logistic regression identified higher median age (OR: 1.08, P = 0.005), and tumors (OR: 5.73, P = 0.035) as risk factors for mortality in n-hmKp infections. Conversely, higher SOFA scores (OR: 2.08, P = 0.000), shock (OR: 48.55, P = 0.001), and elevated levels of procalcitonin (OR: 0.96, P = 0.019), lactic acid (OR: 1.78, P = 0.015), and troponin T (OR: 1.01, P = 0.029) were associated with mortality in hmKp infections. Both hmKp and n-hmKp strains exhibited varying degrees of antibiotic resistance, with n-hmKp strains demonstrating higher resistance to common antibiotics, especially for ceftriaxone, imipenem, and piperacillin-tazobactam compared to hmKp strains (all P < 0.05).</p><p><strong>Conclusions: </strong>N-hmKp and hmKp exhibit significant differences in both clinical and microbiological characteristics. Heightened awareness of them is essential for effective management.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2343-2354"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the VITEK^® REVEAL™ versus the VITEK2 system in susceptibility testing of NDM-producing enterobacterales and Acinetobacter baumannii directly from positive blood cultures.","authors":"Ohad Shalom, Inbar Cohen-Lerner, Amos Adler","doi":"10.1007/s10096-025-05219-0","DOIUrl":"10.1007/s10096-025-05219-0","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the antimicrobial susceptibility testing (AST) results obtained by the VITEK^® REVEAL™ to the VITEK^®2 system in the testing of (1) NDM-producing Enterobacterales (NDME) isolates and (2) NDM-producing Acinetobacter baumannii (NDMAb) isolates.</p><p><strong>Methods: </strong>The study included 197 NDME isolates and 54 NDMAb isolates that were collected in Israel between 2018 and 2019. For the REVEAL™ testing, isolates were suspended in saline, inoculated into a blood culture FA Plus^® bottles and incubated using the VIRTUO^® system until flagged positive. A sample was then transferred into the REVEAL™ GN01 panel and the results were compared with the VITEK^®2 N308 card as reference. Isolates with major or very-major discrepancies (MD and VMD, respectively) between the systems were tested by the Sensititre™ GNX3F Plates.</p><p><strong>Results: </strong>For NDME, high rates of VMD were observed in amikacin (3.8%), imipenem (5.7%) and meropenem (8%) while high rates of MD were observed in trimethoprim-sulfamethoxazole (56.3%), amikacin (15.2%), and aztreonam (29%). Enterobacter cloacae isolates accounted for the majority of errors in carbapenem antimicrobials and aztreonam. Compared to the Sensititre results, REVEAL™ testing displayed lower agreement for ciprofloxacin (0/6) and aztreonam (5%) and higher agreement for trimethoprim-sulfamethoxazole (45%) and meropenem (87%). For NDMAb, there were 35% and 76% minor discrepancies in imipenem and meropenem, respectively. The REVEAL™ results matched the Etest^TM method.</p><p><strong>Conclusion: </strong>Use of the REVEAL™ system can provide rapid AST of positive blood culture. However, testing of NDME isolates revealed several discrepancies compared with the VITEK^TM2 system. E. cloacae isolates accounted for the majority of the discrepancies.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2509-2514"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High co-infection burden and ICU-specific pathogen profiles in pediatric Candida albicans respiratory infections: a large-scale tNGS analysis.","authors":"Ya Huang, Huideng Zhou, Jiangyang Zhao, Lishai Mo, Junming Lu, Guangbing Liu, Shuo Zhang, Yanhua Feng, Wenting Tang, Qiang Huang, Yan Chen, Cuihong Lu, Xuehua Hu, Ruting Chen, Jiaqi Wen, Yanqing Tang, Shang Yi, Hao Wei, Jielin Chen, Huiping Huang, Jie Tan, Chunyun Fu","doi":"10.1007/s10096-025-05216-3","DOIUrl":"10.1007/s10096-025-05216-3","url":null,"abstract":"<p><strong>Background: </strong>Candida albicans is a significant opportunistic pathogen in pediatric respiratory infections, yet systematic analyses of its role in hospitalized children remain scarce. This study evaluates the prevalence, clinical profiles, and outcomes of C. albicans-associated respiratory infections in this population.</p><p><strong>Methods: </strong>From April 2021 to December 2024, 10,310 pediatric inpatients with respiratory infections at Guangxi Zhuang Autonomous Region Maternal and Child Health Hospital underwent pathogen screening via targeted next-generation sequencing (tNGS).</p><p><strong>Results: </strong>C. albicans was detected in 5.0% (511/10,310), with detection rates varying by specimen: highest in sputum (10.3%), followed by bronchoalveolar lavage fluid (8.8%), and minimal in throat swabs (0.3%). Co-infections occurred in 99.0% (506/511), predominantly involving Mycoplasma pneumoniae (189), Haemophilus influenzae (174), and Rhinovirus (169). ICU patients (6.3%) demonstrated elevated inflammatory markers (PCT: 0.31 vs. 0.08 ng/mL; D-dimer: 795 vs. 360 ng/mL FEU; P < 0.001), prolonged hospitalization (median 17 vs. 7 days, P < 0.001), and distinct pathogens (Acinetobacter baumannii vs. M. pneumoniae in non-ICU). 51.7% and 35.4% of the children experienced respiratory and other systemic complications, respectively. Among the 511 children diagnosed with C. albicans infection, 32 (6.3%) required ICU admission for monitoring. The median duration of hospitalization for these 511 children was 7 days. Of these, 482 (94.3%) recovered and were discharged following treatment, while 26 (5.0%) had family members who requested voluntary discharge due to unsatisfactory outcomes or other reasons. Unfortunately, 3 children (0.6%) died despite receiving intensive medical treatment.</p><p><strong>Conclusion: </strong>This large-scale tNGS-based study addresses critical knowledge gaps, revealing ICU-specific pathogen profiles (A. baumannii), high co-infection burdens (99.0%), and complications linked to prematurity/immunodeficiency. The predominance of lower respiratory tract involvement (sputum/BALF positivity) underscores the need for specimen-selective diagnostics. Findings advocate for optimized tNGS protocols, ICU-tailored antimicrobial strategies, and early interventions in high-risk subgroups to reduce morbidity (e.g., sepsis risk) and socioeconomic burdens.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2495-2508"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of centralized hospitalization treatment on transmission in household contacts of pulmonary tuberculosis patients: a contact-traced study.","authors":"Shengqiong Guo, Zhi Zhang, Antonio Avalos Perez, Fangming Xianyu, Jinglei Sheng, Huihui Liu, Yunzhou Ruan, Yan Huang","doi":"10.1007/s10096-025-05170-0","DOIUrl":"10.1007/s10096-025-05170-0","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary tuberculosis (PTB) is a respiratory infectious disease that seriously endangers people's health and incurs high treatment costs, which quickly leads to catastrophic expenditure for patients and their families. A centralized hospitalization treatment (CHT) strategy can be implemented to mitigate the transmission of PTB. This study evaluates the effectiveness of a CHT approach in reducing the magnitude of Mycobacterium tuberculosis (MTB) transmission in household contacts (HHCs) of confirmed PTB cases and explores potential risk factors for PTB.</p><p><strong>Methods: </strong>This retrospective cohort study used PTB cases from Guizhou, China, between January 2022 and October 2023. The HHCs of PTB cases diagnosed etiologically and treated with non-CHT were designated as the exposed group, and the HHCs of those treated with CHT were the non-exposed group. The ratio of the HHCs to index cases was 1:1-3. Face-to-face interviews were conducted for the participants by medical staff at home. R software was used for data analysis. Continuous variables were cut to create new categorical variables and were analyzed using the Chi-square test or Fisher test according to the nature of the data. The risk factors of PTB/LTBI and covariates were analyzed using a multivariate logistic regression model evaluated by the Akaike information criterion (AIC) and elucidated by a Directed Acyclic Graph (DAG). The alpha (α) test level of all statistical tests was 0.05.</p><p><strong>Results: </strong>1007 participants were investigated, including 559 HHCs of PTB index cases from CHT settings and 448 HHCs of PTB index cases from non-CHT sites (treated at home). Of the two groups, 46 HHCs tested positive for PTB/LTBI (latent TB infections), with a 3.4% positive detection rate (19 cases) in the HHCs of PTB index cases treated with CHT and 6.0% (27 cases) in the HHCs of those treated with non-CHT, with positive detection of LTBI [17(3.0%) vs. 26(5.8%)] and [3(0.5%) vs. 5(1.1%)] of PTB in the former than that in the latter. A statistically significant difference was found between the two LTBI groups. In the univariate analysis, family caregivers, age, marital status, CHT, eating the same food with the patient, sleeping in the same room with the patient, and caring for the patient for more than or equal to 2 months were risk factors for PTB/LTBI among HHCs. The treatment of PTB families with non-CHT was an independent factor of PTB/LTBI in the HHCs through multivariate analysis and AIC evaluation.</p><p><strong>Conclusions: </strong>The transmission of PTB/LTBI to HHCs is lower in the HHCs of CHT patients than in the HHCs of those treated with non-CHT after controlling for the other factors including older age, abnormal marriage, and staying with PTB patients equal to or more than two months.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2369-2382"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical features and risk factors for severe macrolide-resistant mycoplasma pneumoniae pneumonia induced by 23 S rRNA A2063G mutation: a retrospective observational study.","authors":"Wenrui Ding, Yan Guo, Houyu Chen, Houxi Bai, Xiaotao Yang, Yanchun Wang, Yonghan Luo","doi":"10.1007/s10096-025-05215-4","DOIUrl":"10.1007/s10096-025-05215-4","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;This study aimed to investigate the clinical characteristics and risk factors of severe macrolide-resistant Mycoplasma pneumoniae pneumonia (MRMP) induced by the 23 S rRNA A2063G mutation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Clinical data were collected from 526 pediatric patients diagnosed with Mycoplasma pneumoniae pneumonia (MPP) at Kunming Children's Hospital, representing a single-center cohort study conducted between October 2023 and February 2024. Among them, 483 cases (91.83%) tested positive for the 23 S rRNA A2063G mutation. Patients were categorized into severe (n = 192) and general (n = 291) groups based on clinical severity. Univariate and multivariate logistic regression analyses were performed to identify risk factors for severe MRMP.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Univariate analysis revealed that the severe group had younger age, longer disease duration, higher peak fever temperatures, and prolonged fever duration compared to the general group. The incidence of wheezing, dyspnea, and decreased breath sounds was significantly higher in the severe group. Radiological findings indicated a higher prevalence of pulmonary consolidation, atelectasis, pleural effusion, and multi-lobar involvement in the severe group. Laboratory tests showed elevated levels of neutrophils, platelets, liver enzymes, lactate dehydrogenase (LDH), D-dimer, and erythrocyte sedimentation rate, alongside reduced levels of albumin, blood urea nitrogen, and creatinine in the severe group. Regarding treatment, doxycycline was the primary alternative for MRMP, but fluoroquinolones were more frequently administered in the severe group, along with a significantly higher usage of glucocorticoids. Additionally, oxygen therapy was more commonly required in the severe group, with 2% of patients necessitating mechanical ventilation or admission to the pediatric intensive care unit. Compared to the general group, the severe group had significantly longer hospital stays (P &lt; 0.01), prolonged lung rales, slower decline in inflammatory markers, and delayed radiological improvement. However, no fatalities were recorded in this cohort. Multivariate logistic regression analysis identified prolonged fever duration, multi-lobar consolidation, elevated LDH, and increased D-dimer levels as independent risk factors for severe MRMP. Receiver Operating Characteristic (ROC) curve analysis demonstrated that the combination of fever duration, multi-lobar consolidation, LDH, and D-dimer had high sensitivity and specificity for diagnosing severe MRMP, with an area under the curve (AUC) of 0.90.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Prolonged fever duration, multi-lobar consolidation, elevated LDH, and increased D-dimer levels are key predictive indicators for severe MRMP. Although severe MRMP is associated with a prolonged clinical course and complex treatment, timely adjustment of antibiotic regimens and supportive care can effectively improve outcomes.&lt;/p&gt;&lt;p&gt;&lt;stro","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2475-2486"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From colonizer to culprit: genomic and clinical insights into S. epidermidis from post-surgical endophthalmitis.","authors":"Susanna Sagerfors, Leandro Andrés Escobar-Herrera, Thor Bech Johannesen, Marc Stegger, Bo Söderquist","doi":"10.1007/s10096-025-05206-5","DOIUrl":"10.1007/s10096-025-05206-5","url":null,"abstract":"<p><strong>Purpose: </strong>To describe the clinical characteristics of exogenous episodes of endophthalmitis from which Staphylococcus epidermidis was isolated by vitreous cultures. We also explored the genomic traits of these S. epidermidis isolates and their relatedness to S. epidermidis originating from carriers and from prosthetic joint infections in the same geographical region.</p><p><strong>Methods: </strong>S. epidermidis isolated from cases of exogenous endophthalmitis (n = 33) were genome sequenced. Clinical features were retrospectively collected from medical records. The isolates were compared with previously sequenced S. epidermidis isolates from the nares of healthy individuals (n = 151) and from prosthetic joint infections (n = 138).</p><p><strong>Results: </strong>The most common ophthalmological procedure preceding the endophthalmitis was a posterior segment surgery (76%; 25/33), mainly intravitreal injection (70%; 23/33). These patients displayed a significantly shorter time to symptoms compared to those with an anterior segment surgery (median 3 vs. 9 days; p < 0.001), and significantly less phenotypic methicillin resistance (8%, n = 2/25 vs. 50%, n = 4/8; p = 0.02). Most isolates of S. epidermidis originating from endophthalmitis cases did not belong to known healthcare-associated lineages and did not cluster with isolates from prosthetic joint infections. Rather, they were more similar to isolates from the nares of healthy individuals.</p><p><strong>Conclusions: </strong>Genomic data suggest that the S. epidermidis isolated from the vitreous of Swedish cases of postsurgical endophthalmitis may originate from the commensal flora of the individual, and not from the healthcare facilities. The type of preceding surgical procedure (anterior vs. posterior segment) may influence both symptom delay and the presence or absence of methicillin resistance.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2409-2420"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of livestock-associated MRSA in the Egyptian Nile Delta that carry the exfoliative toxin gene etA: a case for enhanced surveillance.","authors":"Stefan Monecke, Sindy Burgold-Voigt, Sascha D Braun, Celia Diezel, Elke Müller, Martin Reinicke, Maged El-Ashker, Mayada Gwida, Elisabeth M Liebler-Tenorio, Samar Boswihi, Muhabat A Raji, Shahinda Rezk, Abiola Senok, Ali M Somily, Edet Udo, Ralf Ehricht","doi":"10.1007/s10096-025-05163-z","DOIUrl":"10.1007/s10096-025-05163-z","url":null,"abstract":"<p><strong>Background: </strong>Staphylococcus aureus is a common opportunistic pathogen. Methicillin-resistant strains, MRSA, carry SCCmec elements that include beta-lactam resistance genes mecA/mecC. One globally common lineage, Clonal Complex (CC) 15 failed to evolve MRSA until, in 2016, CC15-MRSA were described from Saudi Arabia that carried a SCCmec V element also comprising the fusidic acid resistance gene, fusC. Henceforth, this strain has spread across Gulf states and Egypt infecting or colonizing both, humans and livestock.</p><p><strong>Methods: </strong>DNA-microarray-based typing was performed on 134 MRSA isolates collected from livestock and farmers in the Nile Delta region of Egypt in 2022. Isolates with conspicuous toxin gene carriage were sequenced applying Oxford Nanopore Technology.</p><p><strong>Results: </strong>Twenty-eight out of 134 isolates were assigned to CC15-MRSA-[V + fusC]. Thus, this strain was the second most common MRSA strain, behind CC88-MRSA-[IV + fusC]. Twenty out of those 28 isolates harboured the gene etA, encoding exfoliative toxin A associated with staphylococcal scalded skin syndrome. Sequencing confirmed the presence of etA on a sufB-integrating prophage. Based on gene content and on electron microscopic morphology after mitomycin C induction, it was assigned to the genus Phietavirus. In addition to SCCmec-borne genes mecA, fusC and aacA-aphD (encoding beta-lactam, fusidic acid and gentamicin/tobramycin resistance), isolates also harboured aadD, lnu(A), tet(K) (for tobramycin, lincosamide and tetracycline resistance) as well as beta-lactamase and cadmium resistance operons on a plasmid. They showed a conspicuous recombination affecting the hsdS/M operon associated with the set/ssl-locus (νSaα). This, and the identity of the composite SCCmec-[V + fusC] element, suggest descent from the etA-negative strain previously observed in Gulf states and Egypt.</p><p><strong>Conclusion: </strong>We describe a novel variant of a CC15 livestock-associated MRSA strain from Egypt. Because of the presence of etA, it might be of increased virulence to humans, especially to new-borns who might also be exposed to contaminated milk. Hence, we urgently recommend surveillance of SSSS/Ritter´s disease in Egypt or in people with relevant travel history.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2383-2400"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular investigation of unexpected Helicobacter pylori bacteremia.","authors":"Claudie Perreau, Anne Gaelle Ranc, Léo Gillet, Tiphaine Gaillard, Lucie Bénéjat, Astrid Ducournau, Johanna Aptel, Quentin Jehanne, Chantal Sobas, Marine Jauvain, Philippe Lehours","doi":"10.1007/s10096-025-05209-2","DOIUrl":"10.1007/s10096-025-05209-2","url":null,"abstract":"<p><p>This study describes the isolation of Helicobacter pylori from a blood sample of an 88-year-old patient with no history or clinical signs suggestive of this infection. We summarize the microbiological characterization of the case, and the subsequent investigation using advanced next-generation sequencing methods, including a target enrichment technique adapted for paraffin-embedded gastric biopsy analysis.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2421-2426"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}