European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"The impact of COVID-19 pandemic on the etiological spectrum of respiratory infections in children.","authors":"Jing Wang, Yuling Han, Xiaoling Wei, Yanqin Liu, Hua Zhang, Xiang Ma","doi":"10.1007/s10096-025-05222-5","DOIUrl":"https://doi.org/10.1007/s10096-025-05222-5","url":null,"abstract":"<p><strong>Objective: </strong>Following the outbreak of the novel coronavirus pandemic, a series of preventive and control measures were adopted by the public, which have had a certain impact on the occurrence of respiratory infectious diseases and changes in their etiology. This article aims to explore the changes in respiratory pathogens among children with respiratory infections during the COVID-19 pandemic and after the comprehensive lifting of restrictions, providing a basis for the clinical diagnosis and treatment of pediatric respiratory infections in the post-pandemic era.</p><p><strong>Methods: </strong>We retrospectively reviewed and analyzed the targeted sequencing results of multiple respiratory pathogens in children with respiratory infections treated at the Children's Hospital affiliated with Shandong University from January 2022 to December 2023.</p><p><strong>Results: </strong>A total of 16,571 targeted sequencing results of pathogens from children with respiratory infections were included in the analysis (2,810 cases in 2022 and 13,761 cases in 2023). The overall positive detection rates of pathogens in 2022 and 2023 were 95.19% and 96.56%, respectively. The positive detection rates for single pathogens were 16.01% vs. 19.29%, while the rates for two or more pathogens were 79.18% vs. 77.27%. The top three viral pathogens with the highest positive detection rates in both 2022 and 2023 were Rhinovirus (RV), Parainfluenza Virus (PIV), and Respiratory Syncytial Virus (RSV). In 2023, the top three bacterial pathogens were Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus, whereas in 2022, they were Streptococcus pneumoniae, Haemophilus influenzae, and Bordetella pertussis. The positive detection rates of Haemophilus influenzae, Staphylococcus aureus, Mycoplasma pneumoniae (MP), RSV, Adenovirus (AdV), Influenza A Virus (IAV), and RV in 2023 were significantly higher than those in 2022 (all P < 0.05). However, the positive detection rates of Streptococcus pneumoniae, Bordetella pertussis, and PIV were significantly lower in 2023 than in 2022 (all P < 0.001). Differences in the positive detection rates of respiratory pathogens were observed across different age groups.</p><p><strong>Conclusion: </strong>Significant changes in the prevalence of certain pathogens occurred during the COVID-19 pandemic and after the lifting of restrictions. It is essential to strengthen long-term monitoring of common respiratory infectious diseases to guide early clinical intervention.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of Enterococcus cecorum in a renal transplant recipient and literature review.","authors":"Valli Mani, Ilan Berlinrut, Frances Wallach, Esther Benamu Sultan, Mersema Abate","doi":"10.1007/s10096-025-05190-w","DOIUrl":"https://doi.org/10.1007/s10096-025-05190-w","url":null,"abstract":"<p><p>Enterococcus cecorum is a bacterium typically found in the gastrointestinal tract of chickens, with rare cases reported in humans. We present a case of Enterococcus cecorum bacteremia in a renal transplant recipient with possible source of pet parrots. We also review the literature on E. cecorum infection in humans.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening of mutations for second-line-drug-resistant genes in Mycobacterium tuberculosis culture isolates by in-house developed DNA bio-chip.","authors":"Bharti Jain, Savita Kulkarni, Nawab Singh Baghel","doi":"10.1007/s10096-025-05221-6","DOIUrl":"https://doi.org/10.1007/s10096-025-05221-6","url":null,"abstract":"<p><strong>Background: </strong>The rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing and is a major setback to TB control in India. The availability of quick and reliable methods for detecting second-line drug resistance (SLDR) is vital to managing patients satisfactorily. A rapid molecular technique to detect SLDR in Mycobacterium tuberculosis (M. tuberculosis) has been developed using DNA biochip.</p><p><strong>Methods: </strong>Specific probes containing wild-type region or specific mutations were designed for immobilization on DNA bio-chip. DNA bio-chip was developed in-house on polycarbonate track-etched membranes (PC-TEM). DNA bio-chip allows the identification of mutations in gyrA gene for fluoroquinolone (FQ) resistance, in rrs gene and the eis promoter region for resistance to second-line injectable drugs (SLID). An asymmetric multiplex PCR was standardized for gyrA, rrs and eis genes. A chemiluminescence based biochip assay was optimized. Bio-chip was tested on 112 M. tuberculosis clinical isolates with different resistance spectra.</p><p><strong>Results: </strong>Isolates analyzed using bio-chip shows that 61 (61%) samples were wild-type. Twelve samples show mutations in gyrA gene, 11 samples in rrs gene, 12 samples in eis gene and 4 samples show double mutation in rrs and eis genes. The sensitivity and specificity of bio-chip for detection of FQ resistance ranged from 75 to 100% and 96.7%-100%, respectively. The sensitivity and specificity of SLID detection ranged from 90.9 to 100% and 96.7-100% respectively. The analytical sensitivity of the bio-chip was ~ 250 genome copies per assay.</p><p><strong>Conclusion: </strong>The biochip has high sensitivity and specificity and could be useful for clinical microbiology studies and epidemiological surveillance of drug resistant (DR) M. tuberculosis. It is a highly accurate tool for screening for SLDR, significantly reducing the time for phenotypic drug susceptibility test (DST) results from weeks to a single day.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical performance of metagenomic next-generation sequencing in the diagnosis of Epstein-Barr virus central nervous system infections.","authors":"Xinfei Yao, Yaping Huang, Lingjun Yuan, Dongsheng Han, Xuan Zhang","doi":"10.1007/s10096-025-05227-0","DOIUrl":"https://doi.org/10.1007/s10096-025-05227-0","url":null,"abstract":"<p><p>Metagenomic next-generation sequencing (mNGS) has been widely utilized for diagnosing infectious diseases. However, studies employing mNGS to detect EBV central nervous system (CNS) infections remain scarce. Therefore, this study aimed to evaluate the diagnostic performance of mNGS in EBV CNS infections. Patients discharged with the diagnosis of CNS viral infection and underwent lumbar puncture for mNGS of cerebrospinal fluid between January 2021 and May 2024 were selected. The selected cerebrospinal fluid samples were subjected to PCR, including those that were EBV-negative in mNGS. Clinical data were collected for analysis. A total of 96 samples were included: 18 samples in the EBV infection group (PCR EBV DNA +), 30 samples in the suspected EBV infection group (PCR EBV DNA - but mNGS +), and 48 samples in the non-EBV-infected group (PCR EBV DNA - and mNGS -). All PCR-positive samples were also positive by mNGS, and in the EBV infection group, the viral load detected by PCR was strongly correlated with the number of sequences identified by mNGS (ρ = 0.752, p = 0.00). The number of sequences in the EBV infection group was higher than that in the suspected EBV infection group (14 vs. 4, p = 0.04). Patients in the EBV high sequence group experienced fewer headaches, nausea, and vomiting, had less elevated intracranial pressure, but required longer hospitalization (p = 0.003, 0.005, 0.03, and 0.03, respectively). The mNGS method is a sensitive tool for detecting EBV CNS infections.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of pneumococcal urinary antigen testing on clinical outcomes in patients hospitalized with community-acquired pneumonia.","authors":"Marita Kern, Markus Fally, Lilian Kolte, Pernille Ravn, Thomas Benfield, Simone Bastrup Israelsen","doi":"10.1007/s10096-025-05226-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05226-1","url":null,"abstract":"<p><p>To establish a microbiological etiology in severe community-acquired pneumonia (CAP), most guidelines recommend adding urinary antigen tests (UAT) to respiratory sampling. However, their usage and impact remain unclear. We examined the clinical impact of UAT in hospitalized CAP patients. This multicenter cohort study included patients admitted with CAP at three hospitals in Denmark from 2017 to 2020. Logistic regression and propensity-score matching were applied to assess the effect of UAT on 30-day mortality, targeted antibiotic treatment and atypical antibiotic coverage. Propensity-score matching was applied to adjust for confounding. Of 2,449 patients included, 654 (27%) had UAT performed within 48 hours of admission. Streptococcus pneumoniae was detected in 8.0% (52/654). 30-day mortality was 11.8% in the tested group and 10.2% in the untested group (adjusted odds ratio [aOR] 1.17, 95% CI 0.83-1.65). Broad-spectrum antibiotics were administered to 47.9% of tested and 42.4% of untested patients (aOR 1.25, 95% CI 1.00-1.55) at discharge. Overall, there were 212 pneumococcal cases, with 30/212 (14%) solely detected by UAT. Of patients with positive pneumococcal UAT, 26% received broad-spectrum antibiotics at discharge, compared with 46% of UAT-negative patients (aOR 0.41, 95% CI 0.18-0.96). Mortality was similar in tested and untested patients. Tested patients received more broad-spectrum antibiotic treatment than untested patients, while patients with positive pneumococcal UAT received more narrow-spectrum antibiotics. Of all pneumococcal cases, 14% were diagnosed exclusively by UAT, suggesting usefulness in antimicrobial stewardship.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of isolation and drug testing methods for Corynebacterium spp. in clinical specimens of granulomatous mastitis.","authors":"Fang Liu, Yewei Yuan, Ruiyang Wu, Ninghua Tang, Kehua Chen, Kui Fan, Wei Cheng, Qu Pan","doi":"10.1007/s10096-025-05220-7","DOIUrl":"https://doi.org/10.1007/s10096-025-05220-7","url":null,"abstract":"","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of mortality and progression from colonization to Stenotrophomonas maltophilia bloodstream infection in hematologic disorders: a single-center retrospective study.","authors":"Hongchen Bi, Qingsong Lin, Qi Sun, Yanxia Lv, Zhiying Tian, Xue Fu, Qiushuang Yan, Yaqing Yu, Hongjing Yao, Fujun Sun, Yonghui Xia, Aiming Pang, Guoqing Zhu, Sizhou Feng","doi":"10.1007/s10096-025-05217-2","DOIUrl":"https://doi.org/10.1007/s10096-025-05217-2","url":null,"abstract":"<p><strong>Background: </strong>Stenotrophomonas maltophilia (S. maltophilia) is an emerging multidrug-resistant pathogen that causes severe infections, particularly in immunocompromised patients. The bloodstream infection (BSI) of S. maltophilia is associated with high mortality rates, especially in patients with hematologic disorders. This study aimed to analyze the clinical characteristics and risk factors of S. maltophilia BSI in patients with hematologic disorders.</p><p><strong>Methods: </strong>We conducted a single-center retrospective study of 107 patients with hematologic disorders (stratified into colonization [n = 41], colonization-to-BSI [n = 37], and primary BSI [n = 29] cohorts) at Hematology Hospital, Chinese Academy of Medical Sciences between January 2021 and September 2024. Data on demographic characteristics, clinical features, laboratory results, treatment regimens, and outcomes were collected from electronic medical records. Blood cultures were performed using the BD BACTEC™ FX system, and bacterial identification was confirmed by MALDI-TOF MS. Antimicrobial susceptibility testing was conducted by the Vitek 2 Compact system. Statistical analyses, including Cox regression and logistic regression, were performed to identify risk factors for mortality.</p><p><strong>Results: </strong>The 30-day mortality rate was 34.8%. Independent risk factors for mortality included prolonged neutropenia (> 1 month) (HR = 1.57, p = 0.04), shock (HR = 2.44, p < 0.01), and ceftazidime resistance (HR = 1.95, p = 0.03). Polymicrobial infections were prevalent (84.8%), and inappropriate initial antibiotic therapy occurred in 42.4% of cases. Progression from colonization to BSI was associated with neutropenia (OR = 6.85, p < 0.001), immunosuppressor use (OR = 7.20, p < 0.001), elevated CRP (> 100 mg/L, OR = 4.11, p = 0.001), and inappropriate antibiotic therapy (OR = 2.98, p = 0.039). A predictive model integrating these factors demonstrated strong performance (AUC = 0.89, 95% CI: 0.82-0.96).</p><p><strong>Conclusion: </strong>S. maltophilia BSI is associated with high mortality, driven by host immunosuppression, antibiotic resistance, and delayed targeted therapy. The progression from colonization to BSI is strongly influenced by neutropenia, immunosuppressor, inflammatory markers, and inappropriate antibiotic use. These findings underscore the need for early risk stratification, and susceptibility-guided therapy in high-risk settings.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and simple detection of Chlamydia trachomatis and Neisseria gonorrhoeae using the EasyNAT CT/NG assay based on cross-priming amplification.","authors":"Qing Wu, Hongxiang Tu, Yingjie Dai, Yumin Wang, Lijuan Hu","doi":"10.1007/s10096-025-05218-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05218-1","url":null,"abstract":"<p><strong>Purpose: </strong>Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) rank among the most common sexually transmitted pathogens. Rapid screening and detection of these bacteria are essential to reduce sequelae and prevent transmission. This study evaluated the efficacy of the EasyNAT CT/NG assay, which utilizes cross-priming amplification (CPA) technique for the rapid and simultaneous detection of CT and NG in diverse reproductive tract specimens, achieving diagnosis within 30 min.</p><p><strong>Methods: </strong>The clinical performance of the EasyNAT CT/NG assay in detecting CT and NG was assessed using 198 clinical samples, with results compared to those of conventional in-house Real-Time PCR to determine concordance. Sensitivity was measured using serial dilutions of quantified plasmids and specificity was evaluated by incorporating DNA from 18 common STI pathogens. The assay's suitability as a point-of-care testing (POCT) tool was evaluated with the criteria outlined in Target Product Profiles (TPPs).</p><p><strong>Results: </strong>The EasyNAT CT/NG assay demonstrated high concordance with Real-Time PCR, with rates of 98.5% for CT and 99.0% for NG. Concordance in urine samples reached 98.6% for CT and 100% for NG, while cervical swabs showed both 97.7% for CT and NG; vaginal and urethral swabs achieved 100% for both pathogens. Among the 198 samples, one urine specimen tested negative for CT by Real-Time PCR but positive by the EasyNAT CT/NG assay, a positive result confirmed by the Cepheid Xpert CT/NG assay. Two cervical swabs, negative for CT and NG by Real-Time PCR, yielded invalid results with the EasyNAT CT/NG assay but were confirmed negative or CT and NG by the Cepheid Xpert CT/NG assay. The EasyNAT CT/NG assay reliably detected CT and NG in turbid specimens, though it may fail with severely hemolytic samples. Its detection limit was 400 copies/mL, with no cross-reactivity observed across 18 other pathogens.</p><p><strong>Conclusion: </strong>The EasyNAT CT/NG assay offers rapid, sensitive, and specific detection of CT and NG, proving valuable for infection screening and early diagnosis. It shows promise as a rapid POCT method.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of carbapenem resistance via MALDI-TOF MS using an innovative broth microgrowth assay.","authors":"Zhenghua Xie, Qun Wang, Ziyuan Zhou, Weixing Wu, Xiaoyu Zhang, Li Zhang","doi":"10.1007/s10096-025-05213-6","DOIUrl":"https://doi.org/10.1007/s10096-025-05213-6","url":null,"abstract":"<p><strong>Objectives: </strong>Carbapenem-resistant Enterobacterales (CRE) are a growing threat to human health worldwide. This study aimed to develop a novel method, the broth microgrowth assay, for the rapid identification of CRE from culture isolates using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).</p><p><strong>Methods: </strong>A total of 80 isolates, including carbapenem-resistant Escherichia coli (CRECO, n = 28), carbapenem-sensitive Escherichia coli (CSECO, n = 28), carbapenem-resistant Klebsiella pneumoniae (CRKP, n = 12), and carbapenem-sensitive Klebsiella pneumoniae (CSKP, n = 12) were collected for this study. The minimum inhibitory concentrations (MICs) of imipenem and meropenem were measured for all the isolates via the broth microdilution method. Carbapenem resistance (R) and susceptibility (S) were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing, M100, 34th Edition. These isolates were incubated both with carbapenem antibiotics (imipenem and meropenem) as a detection site and without antibiotics as a growth control site at 35°C for 1 and 2 h, respectively. Following incubation, the mixtures were centrifuged, and the supernatant was pipetted off. The remaining sediment was subsequently applied to the MALDI-TOF MS target plate, and the residual broth was subsequently absorbed via sterile filter paper. Identification of the isolates was performed via the VITEK MS system. The test was deemed valid only if the sample without antibiotics (growth control) resulted in successful species identification (confidence level > 60.0%). Percentage of isolates that successfully grew without antibiotics and were identified by MALDI-TOF MS, which is referred to as the growth efficiency. CRE were distinguished if the microorganisms could be successfully identified.</p><p><strong>Results: </strong>After 1 h of incubation with imipenem or meropenem, the growth efficiencies of E. coli and K. pneumoniae were 83.93% and 66.67%, respectively. For Escherichia coli, the sensitivity and specificity of imipenem resistance prediction by MALDI-TOF MS were 82.14% and 100%, respectively. Conversely, meropenem demonstrated a sensitivity of 89.29% and a specificity of 100%. When Klebsiella pneumoniae was examined, both imipenem and meropenem had sensitivity and specificity values of 83.33% and 100%, respectively. After the incubation time was extended to 2 h, both antibiotics achieved perfect sensitivity and specificity of 100%, coupled with a growth efficiency of 100% for both bacterial strains.</p><p><strong>Conclusion: </strong>Combining the broth microgrowth assay with MALDI-TOF MS offers a rapid and accurate approach to identifying CRE, thus facilitating the swift selection of appropriate antibiotics.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the VITEK^® REVEAL™ versus the VITEK2 system in susceptibility testing of NDM-producing enterobacterales and Acinetobacter baumannii directly from positive blood cultures.","authors":"Ohad Shalom, Inbar Cohen-Lerner, Amos Adler","doi":"10.1007/s10096-025-05219-0","DOIUrl":"https://doi.org/10.1007/s10096-025-05219-0","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the antimicrobial susceptibility testing (AST) results obtained by the VITEK^® REVEAL™ to the VITEK^®2 system in the testing of (1) NDM-producing Enterobacterales (NDME) isolates and (2) NDM-producing Acinetobacter baumannii (NDMAb) isolates.</p><p><strong>Methods: </strong>The study included 197 NDME isolates and 54 NDMAb isolates that were collected in Israel between 2018 and 2019. For the REVEAL™ testing, isolates were suspended in saline, inoculated into a blood culture FA Plus^® bottles and incubated using the VIRTUO^® system until flagged positive. A sample was then transferred into the REVEAL™ GN01 panel and the results were compared with the VITEK^®2 N308 card as reference. Isolates with major or very-major discrepancies (MD and VMD, respectively) between the systems were tested by the Sensititre™ GNX3F Plates.</p><p><strong>Results: </strong>For NDME, high rates of VMD were observed in amikacin (3.8%), imipenem (5.7%) and meropenem (8%) while high rates of MD were observed in trimethoprim-sulfamethoxazole (56.3%), amikacin (15.2%), and aztreonam (29%). Enterobacter cloacae isolates accounted for the majority of errors in carbapenem antimicrobials and aztreonam. Compared to the Sensititre results, REVEAL™ testing displayed lower agreement for ciprofloxacin (0/6) and aztreonam (5%) and higher agreement for trimethoprim-sulfamethoxazole (45%) and meropenem (87%). For NDMAb, there were 35% and 76% minor discrepancies in imipenem and meropenem, respectively. The REVEAL™ results matched the Etest^TM method.</p><p><strong>Conclusion: </strong>Use of the REVEAL™ system can provide rapid AST of positive blood culture. However, testing of NDME isolates revealed several discrepancies compared with the VITEK^TM2 system. E. cloacae isolates accounted for the majority of the discrepancies.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}