European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Desensitisation protocol for ethambutol in the treatment of Mycobacterium avium complex pulmonary disease.","authors":"George Denby, Michael Holt, Malcolm Wilson, Victoria Grey, Andrew Burke, Rachel Thomson, Ieuan Edward Shepherd Evans","doi":"10.1007/s10096-026-05702-2","DOIUrl":"https://doi.org/10.1007/s10096-026-05702-2","url":null,"abstract":"<p><strong>Background: </strong>Ethambutol is a key component of treatment for Mycobacterium avium complex pulmonary disease (MAC-PD), primarily through suppression of macrolide resistance. However, adverse drug reactions frequently lead to treatment interruption, and evidence-based alternatives remain limited. We evaluated the feasibility and outcomes of a structured outpatient ethambutol desensitisation protocol in patients with MAC-PD who developed ethambutol intolerance or hypersensitivity.</p><p><strong>Methods: </strong>We conducted a multicentre observational study across four specialist NTM centres. Patients undergoing treatment for MAC-PD who experienced an ethambutol-related adverse reaction leading to drug cessation were eligible. A patient-led outpatient desensitisation protocol commenced at 25 mg daily with gradual dose escalation to a weight-based target dose. The protocol allowed temporary dose reduction to previously tolerated levels if symptoms recurred. Success was defined as achievement of the target dose or maintenance of a tolerated dose associated with sustained culture conversion.</p><p><strong>Results: </strong>Twenty desensitisation episodes were performed in 17 patients (65% female; mean age 72 years). The most common indication was rash (70%), followed by headache (15%). Overall, 17/20 (85%) desensitisations were successful, including 13/14 (93%) among patients with delayed cutaneous reactions. Mean time to target dose was 9 weeks (median 4 weeks). No severe immediate hypersensitivity reactions occurred during desensitisation. Sixteen episodes (80%) were associated with culture conversion, and 11 (55%) patients completed therapy with ethambutol-containing regimens.</p><p><strong>Conclusions: </strong>Outpatient ethambutol desensitisation appears feasible, safe and effective in selected patients with MAC-PD and non-severe ethambutol intolerance. This approach may help preserve an essential component of MAC therapy when treatment options are otherwise limited.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ST20 Staphylococcus aureus causing suppurative cervical lymphadenitis in an extremely low-birth-weight infant.","authors":"Yuya Fukuda, Atsuo Togashi, Meiji Soe Aung, Hikaru Takahashi, Yui Ito, Nao Harada, Kaori Miki, Takeshi Nishida, Shintaro Fusagawa, Takuro Sakai, Nobumichi Kobayashi, Takeshi Tsugawa","doi":"10.1007/s10096-026-05699-8","DOIUrl":"https://doi.org/10.1007/s10096-026-05699-8","url":null,"abstract":"<p><p>Neonatal suppurative cervical lymphadenitis is uncommon, particularly in extremely low-birth-weight infants. We report a 772 g infant born at 28 weeks of gestation who developed rapidly progressive unilateral cervical lymphadenitis caused by methicillin-susceptible Staphylococcus aureus (MSSA) on postnatal day 31. The infant remained afebrile and blood cultures were negative despite progression requiring repeated drainage. The cervical isolate was genotyped as ST20/spa-t3929 and shared an identical genetic profile with an earlier pharyngeal isolate, suggesting that the infection arose from pre-existing S. aureus colonization. This case highlights the potential for ST20 MSSA to cause localized invasive infection in preterm infants.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bismuth-containing quadruple therapy for Helicobacter pylori infection in penicillin‑allergic children: efficacy, safety and real‑world experience from China.","authors":"Ying Zhou, Jianping Xu, Yuhuan Wang, Zhiheng Huang, Ying Huang","doi":"10.1007/s10096-026-05696-x","DOIUrl":"https://doi.org/10.1007/s10096-026-05696-x","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the efficacy and safety of 14-day bismuth-containing quadruple therapy (BQT) as both first-line and rescue treatment for children with Helicobacter pylori infections and penicillin allergies in China.</p><p><strong>Methods: </strong>This real-world, exploratory, retrospective observational cohort study enrolled children aged 6 to 18 years who underwent gastroendoscopy. The eradication status was assessed at least 4 weeks after treatment completion. A negative urea breath test or endoscopic rapid urease test result indicated successful eradication.</p><p><strong>Results: </strong>Between July 2017 and June 2025, 453 patients were analyzed in the study. Metronidazole-clarithromycin BQT as first-line treatment achieved eradication rates of 81.2% (285/351; 95% confidence interval [CI], 77.1-85.3%) and 82.5% (279/338; 95% CI, 78.5-86.6%) in modified intention-to-treat (mITT) and per-protocol (PP) analyses. Metronidazole-clarithromycin BQT as rescue therapy succeeded in 41.4% (41/99; 95% CI, 31.7-51.1%) of cases in mITT and in 42.6% (40/94; 95% CI, 32.6-52.5%) of cases in PP analyses. Three patients treated with metronidazole-minocycline BQT as rescue therapy all achieved successful eradication of H. pylori. Adverse effects were reported by 12.8% (45/351) of patients in the first-line therapy group and 9.8% (10/102) in the rescue therapy group (p = 0.40). The compliance rates for metronidazole-clarithromycin BQT as first-line therapy and rescue therapy were 96.3% (338/351) and 94.9% (94/99), respectively.</p><p><strong>Conclusion: </strong>As first-line therapy, metronidazole-clarithromycin BQT achieved an eradication rate of 82.5% in Chinese children with penicillin allergies. However, this regimen was inadequate as a rescue treatment. Rescue regimens such as those containing minocycline or cefuroxime warrant further investigation.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Scalp eschar and neck lymphadenopathy after tick bite: an emerging syndrome with multiple causes.","authors":"G Dubourg, C Socolovschi, P Del Giudice, P E Fournier, D Raoult","doi":"10.1007/s10096-026-05690-3","DOIUrl":"https://doi.org/10.1007/s10096-026-05690-3","url":null,"abstract":"","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of urine concentration and automatic interpretation on sensitivity and specificity of Legionella BinaxNOW^® results.","authors":"Camille Jacqueline, Ghislaine Descours, Anne-Gaëlle Ranc, Coralie Espinasse, Sophie Jarraud, Laetitia Béraud","doi":"10.1007/s10096-026-05692-1","DOIUrl":"https://doi.org/10.1007/s10096-026-05692-1","url":null,"abstract":"<p><strong>Purpose: </strong>Urinary antigen tests (UATs) are widely used for rapid diagnosis of Legionnaires' disease (LD), primarily detecting Legionella pneumophila serogroup 1 lipopolysaccharide (LPS). Strategies, such as urine concentration, were shown to improve performance but mostly in retrospective studies.</p><p><strong>Methods: </strong>We evaluated the impact of urine concentration on the diagnostic sensitivity and specificity of the BinaxNOW^® assay. A set of 90 positive and 200 negative urine samples were prospectively collected. Another set of 48 positive urine samples were selected retrospectively to evaluate both urine concentration but also automated interpretation using the Digival™ reader on test sensitivity and specificity.</p><p><strong>Results: </strong>We found that urine concentration significantly increased sensitivity by 9% before heat treatment. Automated interpretation increased sensitivity by 16.3% in unconcentrated samples. Before heat treatment, specificity remained high with visual interpretation (100% unconcentrated) but slightly decreased with automated reading (98% unconcentrated). After heat treatment, specificity reached 100% for all methods. In LPS-spiked samples, automated interpretation improved analytical sensitivity by 2- to 10-fold, particularly for mAb 3/1-positive strains.</p><p><strong>Conclusion: </strong>Both urine concentration and automated interpretation with the Digival™ reader increased the sensitivity of the BinaxNOW^® assay. Automated interpretation achieved this without the additional processing step required for concentration and may therefore represent a practical alternative. Heat treatment of positive samples remains necessary to maintain optimal specificity.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-related differences in clinical characteristics, management, and outcomes of Enterococcus faecalis bloodstream infection.","authors":"Laura Escolà-Vergé, Miguel Villamarín, Anna Falcó-Roget, Cristina Kirkegaard-Biosca, Belén Viñado, María Nieves Larrosa, Alba Rivera, Pol Duch-Llorach, Sara Grillo, Joaquín López-Contreras, Nuria Fernández-Hidalgo","doi":"10.1007/s10096-026-05694-z","DOIUrl":"https://doi.org/10.1007/s10096-026-05694-z","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate whether clinical characteristics and outcomes of patients with Enterococcus faecalis bloodstream infection (EF-BSI) differ by biological sex.</p><p><strong>Methods: </strong>Quasi-experimental study conducted at two hospitals, comprising a retrospective phase (2017-2019) and a prospective intervention phase (2022-2024), including adults with a first episode of monomicrobial EF-BSI. Patients who died within 72 h or were receiving palliative care were excluded. A structured management bundle was implemented in the intervention phase. Clinical features, bundle adherence, and 90-day mortality were compared by sex. Cox regression was used to assess the association between sex and 90-day mortality.</p><p><strong>Results: </strong>Among 250 patients, 77 (31%) were females and 173 (69%) males. Females were younger than males (median 63 [50-76] vs. 73 [63-80] years; p < 0.001). Cardiovascular disease was more frequent in males (30 [39%] vs. 94 [54%]; p = 0.025), whereas immunosuppressive therapy (29 [38%] vs. 35 [20%]; p = 0.004) and connective tissue disease (9 [12%] vs. 2 [1%]; p < 0.001) were more frequent in females. Persistent bacteraemia (2/57 [4%] vs. 30/139 [22%]; p = 0.002) and infective endocarditis (14 [18%] vs. 59 [34%]; p = 0.011) were more frequent in males. Females received fewer bundle measures (5 [3-6] vs. 5 [4-6]; p = 0.028), largely because echocardiography and colonoscopy were less frequently indicated. Ninety-day mortality was 22% (17) in females and 14% (25) in males (p = 0.136). Female sex was associated with higher 90-day mortality after adjustment (aHR 2.00, 95%CI 1.04-3.83).</p><p><strong>Conclusions: </strong>Further studies should explore the underlying causes of these differences and potential implications for individualized management.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of proven Fusarium brachygibbosum sinusitis in a paediatric patient with acute myeloblastic leukaemia.","authors":"Alfredo Maldonado-Barrueco, Yasmina Mozo Del Castillo, Fernando Baquero-Artigao, Carlos Grasa, Noorelain Del Valle de León, Isabel Martínez-Romera, Iñigo Figueroa-Real de Asua, Ana Alastruey-Izquierdo, Laura Alcázar-Fuoli, Inmaculada Quiles-Melero, Julio García-Rodríguez","doi":"10.1007/s10096-026-05695-y","DOIUrl":"https://doi.org/10.1007/s10096-026-05695-y","url":null,"abstract":"<p><strong>Purpose: </strong>Fusarium brachygibbosum is an environmental mould belonging to the Fusarium sambucinum species complex (FSAMSC) and is only exceptionally reported as a human pathogen. Invasive fusariosis is a recognized complication in patients with hematological malignancies; however, proven infection caused by F. brachygibbosum has not previously been described. We report the first case of proven breakthrough sinusitis in a pediatric female with refractory acute myeloblastic leukaemia (AML) receiving posaconazole prophylaxis.</p><p><strong>Methods: </strong>We describe the clinical presentation, microbiological diagnosis, antifungal susceptibility profile, treatment, and outcome of a 13-year-old neutropenic female with refractory AML who developed invasive fungal sinusitis. Species identification was established by Sanger sequencing of the elongation factor 1-alpha, and antifungal susceptibility testing was performed according to the EUCAST reference method.</p><p><strong>Results: </strong>Computed tomography revealed extensive paranasal sinus involvement, and direct microscopy of sinus tissue demonstrated hyaline septate hyphae. Culture yielded a mould initially misidentified by MALDI-TOF MS as F. chlamydosporium. Molecular sequencing confirmed F. brachygibbosum. The isolate exhibited elevated Minimal inhibitory concentrations (MICs) to amphotericin B, azoles, echinocandins, and olorofim, while fosmanogepix showed a lower in vitro MIC. Extensive surgical debridement combined with liposomal amphotericin B, voriconazole, and subsequently olorofim was performed. Despite local control of the sinonasal infection and no microbiological or histopathological evidence of dissemination, the patient died 23 days after diagnosis. The death occurred in the context of progressive refractory AML, invasive fungal disease, pulmonary abnormalities, and subsequent neutropenic complications.</p><p><strong>Conclusion: </strong>This report represents the first proven pediatric case of invasive sinusitis caused by F. brachygibbosum (FSAMSC) isolate with higher MICs to classical antifungals. The case highlights the diagnostic limitations of MALDI-TOF MS for rare Fusarium species, the value of molecular identification and antifungal susceptibility testing, and the emergence of environmental moulds breakthrough fungal infections. It also underscores the urgent need for effective novel antifungal agents against resistant Fusarium species.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and serological outcomes of syphilis: an 18-year retrospective cohort study on predictors of treatment response and seroreversion in Türkiye.","authors":"Hatice Sertkaya, Esra Kazak, Ayşe Melda Payaslıoğlu, Uğur Önal, Yasemin Heper, Emel Yılmaz, Emin Halis Akalın","doi":"10.1007/s10096-026-05698-9","DOIUrl":"https://doi.org/10.1007/s10096-026-05698-9","url":null,"abstract":"<p><strong>Purpose: </strong>Despite established protocols, the global resurgence of syphilis remains a public health challenge, with outcomes influenced by disease stage and host factors. This study described 18-year epidemiological trends and identified predictors of serological treatment response and long-term seroreversion.</p><p><strong>Methods: </strong>We retrospectively analyzed 503 adults diagnosed with syphilis between 2005 and 2022 and classified disease stages according to CDC criteria. Treatment response was defined as a ≥ 4-fold decline in rapid plasma reagin (RPR) titers within 12 months for early-stage and 24 months for late-stage syphilis, while seroreversion was defined as a non-reactive RPR. Overall, 157 patients were included in the treatment-response analysis and 159 in the seroreversion analysis, the latter including two additional patients who achieved seroreversion before treatment-response assessment. Independent predictors were identified using multivariable logistic regression.</p><p><strong>Results: </strong>Syphilis cases increased significantly after 2015, with late-stage disease accounting for 75.7% (n = 381) of diagnoses. Early syphilis rose from 10.5% (2005-2015) to 30.2% after 2015 (p < 0.001). Among patients with available HIV test results, 29.4% (134/456) of cases were in people with HIV. Early-stage syphilis was more frequent in people living with HIV (PLWH) than in HIV-negative individuals (32% vs. 20.1%). Among patients with follow-up, 75.2% (118/157) achieved a serological response. In multivariate analysis, late-stage syphilis was a negative predictor of response (OR 0.22, 95% CI 0.07-0.76; p = 0.016), while baseline RPR titers ≥ 1:8 were a strong positive predictor (OR 8.18, 95% CI 1.72-38.85; p = 0.008). HIV status was not independently associated with response (p > 0.05). Seroreversion occurred in 35.8%, with no independent predictors identified.</p><p><strong>Conclusions: </strong>The syphilis burden has grown, driven by delayed diagnosis and late-stage predominance despite recent trends toward earlier detection. In this cohort, disease stage and baseline RPR titers were stronger predictors of serological response than HIV status. The higher frequency of early-stage diagnosis in PLWH is consistent with the potential impact of intensified screening and may reflect differences in healthcare engagement and testing practices compared with the general population.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical burden and outcomes of pediatric carbapenem-resistant Klebsiella pneumoniae bloodstream infections in a tertiary care center.","authors":"Sevliya Öcal Demi̇r, Seyhan Yilmaz, Meryem Çağla Abacı Çapar, Sıla Cura, Faruk Odabaşi, Ahmet Furkan Eser, Ezgi Çelikboya, Arzu İlki̇, Eda Kepenekli̇, Gülşen Akkoç","doi":"10.1007/s10096-026-05697-w","DOIUrl":"https://doi.org/10.1007/s10096-026-05697-w","url":null,"abstract":"<p><strong>Background: </strong>Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an emerging threat in pediatric healthcare settings because of limited treatment options and high morbidity and mortality. However, data regarding pediatric CRKP bloodstream infection (BSI) remain limited. This study aimed to evaluate the clinical characteristics and outcomes of CRKP BSI in children.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted among children hospitalized with Klebsiella pneumoniae BSI between 2020 and 2025. Demographic characteristics, healthcare exposures, laboratory findings, antimicrobial therapy, and clinical outcomes were analyzed.</p><p><strong>Results: </strong>A total of 86 children were included; the median age was 12 months, and 50% were male. Overall, 91.9% had at least one underlying comorbidity, 89.5% had a history of previous hospitalization, 83.3% had prior antibiotic usage, and 25.9% had undergone surgical intervention. During the index hospitalization, 57.0% required intensive care unit admission and 77.6% had at least one invasive device. Fifteen children (17.4%) had CRKP BSI. Prior carbapenem exposure and longer antibiotic use were more frequent in the CRKP group, although the differences were not statistically significant. White blood cell, neutrophil, lymphocyte, and platelet counts were significantly lower in patients with CRKP BSI. Three-month mortality was 40.0% in the CRKP group and 15.9% in the CSKP group, with no statistically significant difference (p = 0.069). Length of hospital stay was also similar between the groups.</p><p><strong>Conclusion: </strong>CRKP accounted for 17.4% of K. pneumoniae BSIs in this pediatric cohort. No independent factor associated with carbapenem resistance was identified. The small number of CRKP cases limited the statistical power of the study. Larger multicenter studies are needed to better define factors associated with CRKP and its clinical outcomes in children.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of acute dengue immune status and serotype surveillance in three regions of Burkina Faso: a hospital-based cross-sectional study.","authors":"Louis Robert Wendyam Belem, Arsène Zongo, Raymond Karlhis Yao, Christelle Adelaide Zilsamba Lankouande, Armand Vital Wenceslas Taïta, Tarwendpanga François Xavier Ouédraogo, Benewêndê Philippe Kabore, Abdoul Bamogo, Benewêndê Philippe Kabore, Abdoul Bamogo, Raïssa Muriel de Souza, Yacouba Kouadima, Dieudonné Tialla, Antoinette Kaboré, Adama Sanou","doi":"10.1007/s10096-026-05693-0","DOIUrl":"https://doi.org/10.1007/s10096-026-05693-0","url":null,"abstract":"<p><strong>Introduction: </strong>In recent years, dengue has become a significant public health concern in Burkina Faso, associated with severe disease and death. Rapid characterization of acute dengue serostatus is useful for identifying patients at risk, informing transmission dynamics, and supporting public health decision-making. This study aimed to characterize dengue immune status in three regions of Burkina Faso to inform pre-vaccination screening, evaluate a commercial combined DENV NS1, IgM, and IgG rapid diagnostic test (RDT) kit, and investigate DENV serotype circulation.</p><p><strong>Methods: </strong>A multicenter study was conducted between 2023 and 2025 in Bobo-Dioulasso, Houndé, Ouagadougou, and Gaoua. Blood samples were collected from patients with suspected dengue. Acute DENV infections and serotypes were identified using real-time RT-PCR, and immune status was characterized by enzyme-linked immunosorbent assay (ELISA) according to WHO criteria. Serum samples were screened using the reference standard (RT-PCR combined with ELISA) to evaluate the performance of a commercial DENV NS1, IgM, and IgG RDT kit in characterizing dengue immune status.</p><p><strong>Results: </strong>Primary dengue accounted for 75.9%, 80.4%, and 71.4% in 2023, 2024, and 2025, respectively. The sensitivity of the RDT for determining primary and post-primary dengue during the first 5 days of illness was 93.3% and 94.7%, respectively. DENV-1, DENV-2, and DENV-3 accounted for 47.0%, 5.6%, and 47.4% of cases, respectively. DENV-4 was not detected. The diagnostic specificity of the RDT for both primary and post-primary dengue was 100%.</p><p><strong>Conclusions: </strong>Primary dengue cases predominated, while severe dengue was associated with post-primary infection. The RDT proved to be a useful point-of-care tool for discriminating dengue immune status. DENV-3 and DENV-1 were the predominant serotypes driving recent transmission dynamics. The findings may help inform future vaccination strategies.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149135404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}