European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Isolation and whole-genome characterization of multidrug-resistant Acinetobacter baumannii from Indian retail meat: unveiling high-risk clones and zoonotic transmission threats via comparative genomics.","authors":"Vanishree B, Wilfred Ruban Savariraj, Mohan H V, Jaganatha Rao, Porteen Kannan, Anbazhagan Subbaiyan, Sureshkannan S, Nidhi Sudhakaran P, Nithya Quintoil M, Sivachandiran R, Sivaraman Gopalan Krishnan, Raghavendra G Amachawadi","doi":"10.1007/s10096-025-05253-y","DOIUrl":"https://doi.org/10.1007/s10096-025-05253-y","url":null,"abstract":"<p><p>Acinetobacter baumannii is a multidrug-resistant (MDR) pathogen increasingly detected in livestock and food products, raising concerns about zoonotic transmission. This study characterized A. baumannii isolates from retail meat sources in India, assessing their genomic diversity, antimicrobial resistance (AMR), virulence factors (VFs), and mobile genetic elements (MGEs). Nine A. baumannii isolates from chicken, buffalo, pork, mutton, and prawn were identified via CHROMagar™, biochemical tests, BD Phoenix™ M50, and rpoB PCR. Antibiotic susceptibility was determined using MIC assays. Whole-genome sequencing (WGS) was performed, followed by phylogenetic, resistome, virulome, and mobilome analyses with global animal, avian and meat isolates. All nine isolates exhibited resistance to β-lactams (amoxicillin-clavulanic acid, ampicillin, aztreonam, cefazolin, cefoxitin) but remained susceptible to tetracycline, amikacin, ciprofloxacin, and carbapenems. Genomic analysis revealed 3.67-3.9 Mbp genomes with 27-49 contigs, harboring 17-22 AMR genes per isolate. Global phylogenetic analysis identified two major clades with host- and country-specific clustering (e.g., ST-1512-OCL2-KL9 in Indian retail chicken, ST-25-OCL6-KL14 in French companion animals). Core VFs included biofilm formation (ompA, csuA-E, pgaA-D), iron acquisition (basA-J, bauA-F), and LPS biosynthesis genes. Plasmid prediction identified 242 plasmids (55 conjugative, 32 mobilizable), with 112 carrying AMR genes (AAC, APH, sul2, tet(B)). MGEs (IS, Tn, ICE) were linked to bla_OXA-23, bla_NDM-1, and aminoglycoside resistance genes. Capsular (KL) and lipooligosaccharide (OCL) loci showed geographic and host-specific distributions (e.g., KL14-OCL6 in France, KL19-OCL1 in UK livestock). Retail meat serves as a reservoir for MDR A. baumannii, with genomic features mirroring clinical strains. The presence of high-risk clones (IC-2, IC-5), carbapenem resistance genes (bla_OXA-23, bla_NDM-1), and mobilizable AMR plasmids underscores the risk of zoonotic transmission. A One Health approach integrating WGS-based surveillance is critical to mitigate the spread of AMR A. baumannii through food chains.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring heterogeneity in antibiotic choices for bloodstream infections caused by AmpC-producing enterobacterales: results from a case-vignette study.","authors":"Gianpiero Tebano, Caterina Campoli, Marianna Meschiari, Ilaria Contadini, Sara Grasselli, Claudia Lazzaretti, Martina Maritati, Luigi Raumer, Matteo Rinaldi, Nicolò Rossi, Kristian Scolz, Elisa Vanino, Cristina Mussini, Pierluigi Viale, Francesco Cristini","doi":"10.1007/s10096-025-05293-4","DOIUrl":"https://doi.org/10.1007/s10096-025-05293-4","url":null,"abstract":"<p><strong>Purpose: </strong>The treatment of bloodstream infections (BSIs) caused by AmpC-producing Enterobacterales (AmpC-E) remains controversial, due to limited clinical evidence and variable resistance profiles. Inappropriate antibiotic choices can induce ampC-encoding gene overexpression, leading to increased risk of therapeutic failure. This study aims to explore the current prescribing preferences among infectious disease (ID) physicians, in complex clinical scenarios involving BSIs caused by AmpC-E.</p><p><strong>Methods: </strong>A web-based case-vignette survey was conducted among a purposive sample of senior ID physicians from 10 ID units in Emilia-Romagna Region, northern Italy. Participants responded to 31 vignettes simulating BSIs caused by Enterobacter cloacae (wild-type or derepressed phenotype) or Serratia marcescens (wild-type phenotype), across varied clinical settings. For each scenario, participants selected their preferred antibiotic regimen.</p><p><strong>Results: </strong>Forty-two ID physicians were invited to participate. Cefepime (48.4%) and meropenem (29.0%) were the most prescribed agents, followed by piperacillin-tazobactam (16.1%) and third-generation cephalosporins (3GC) (6.5%). Cefepime was preferred in milder cases, while meropenem in severe infections, particularly with high inoculum or incomplete source control. Considerable heterogeneity in treatment choices was observed, especially for S. marcescens and E. cloacae with wild-type phenotypes. Agreement among clinicians was highest for intensive care admitted patients. Combination therapies were infrequently proposed (8.8% overall), primarily in the setting of intra-abdominal infections with incomplete source control or ventilator-associated pneumonia.</p><p><strong>Conclusion: </strong>This study highlights significant variability in the treatment of AmpC-E BSIs among ID physicians, reflecting clinical uncertainty and lack of high-quality evidence. Further research and updated, context-specific guidelines are needed to harmonize practice and promote optimal antibiotic stewardship.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcus dysgalactiae as a cause of peripartum infections - a population-based cohort study with phylogenetic analysis of hospital clusters.","authors":"Matilda Dooley, Erik Senneby, Omar Sigurvin Gunnarsson, Anja Carblom, Ann-Cathrine Petersson, Magnus Rasmussen","doi":"10.1007/s10096-025-05284-5","DOIUrl":"https://doi.org/10.1007/s10096-025-05284-5","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to investigate the incidence and clinical features of Streptococcus dysgalactiae peripartum infections (SDPI), and to investigate the distribution of emm-types in relation to disease severity and the genetic relatedness of isolates from hospital clusters.</p><p><strong>Materials and methods: </strong>Patients with growth of S. dysgalactiae in a genital or wound culture, collected between January 2014 and September 2020 at departments for gynecology and obstetrics, were identified. For inclusion, patients had to be pregnant, or given birth, or undergone an abortion within 42 days prior to debut of symptoms. All isolates had previously been emm-typed. A cluster was defined as two or more patients with S. dysgalactiae of the same emm-type admitted to the same hospital within a 30-day period. The cluster isolates were subjected to whole genome sequencing (WGS).</p><p><strong>Results: </strong>The final study cohort comprised 130 patients. The incidence of S. dysgalactiae postpartum infection was approximately 1 case/1000 births. The patients fulfilled criteria for endometritis (n = 94), postpartum fever (n = 15), wound infection (n = 8) or chorioamnionitis (n = 4). Most patients with endometritis (87%) had onset of symptoms > 48 h post-partum. The most common emm-type was stG62647 (n = 41). Thirteen hospital clusters were identified, of which only three had bacterial isolates that were closely genetically related (0-6 SNP).</p><p><strong>Conclusion: </strong>Our findings demonstrate that SDPI impact a relatively large number of patients. No patient was critically ill, but the morbidity appeared to be substantial. Hospital outbreaks of SDPI are rare, but when suspected, WGS should be employed to investigate relatedness between isolates.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of an institutional sepsis guideline on selecting appropriate empirical treatment in patients with carbapenem-resistant gram-negative bacilli bacteremia.","authors":"Hanife Uzar, Eda Karadoğan, Ahmet Sertçelik, Gülçin Telli Dizman, Gülşen Hazırolan, Ömrüm Uzun, Banu Çakır, Gökhan Metan","doi":"10.1007/s10096-025-05276-5","DOIUrl":"https://doi.org/10.1007/s10096-025-05276-5","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to investigate the impact of our institutional sepsis protocol on the empirical treatment of carbapenem-resistant Gram-negative bacteria in a setting where infectious disease consultation (ID) is available 7 days / 24 h and broad-spectrum antibiotic use requires ID approval.</p><p><strong>Methods: </strong>A total of 612 patients (168 patients pre-guideline, 444 patients post-guideline) who received empirical antibiotics for suspicion of sepsis before documentation of antibacterial susceptibility were included. Demographic, clinical and microbiological data were collected from the hospital's electronic medical record system, retrospectively. Compliance with institutional guidelines and the rate of appropriate antibiotic use prior to the availability of antibiograms were assessed.</p><p><strong>Results: </strong>There was a statistically significant increase in the utilization rate of empirical antibacterial treatment based on pre-defined risk factors of multidrug resistance [OR (95% CI) 1.73 (1.21-2.48), p = 0.003]. Furthermore, appropriateness of the initial antibacterial treatment according to the antibiogram results increased significantly in the post-guideline period [OR (95% CI) = 3.25 (2.09-5.06), p < 0.001]. The rate of compliance with guideline recommendations (p = 0.004) and the rate of appropriate empirical antibiotic treatment (p < 0.001) by each year were significant when compared with the pre-guideline period. Also, practices that improve drug pharmacokinetics such as loading dose, prolonged infusion of meropenem and adjusting antibiotic doses according to renal function increased statistically after the release of guideline.</p><p><strong>Conclusion: </strong>An institutional sepsis protocol based on risk factors for multidrug resistance and local epidemiology increased the rate of appropriate empirical antibiotic treatment even in a setting where ID consultation is readily available.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical application of metagenomic next-generation sequencing (mNGS) in children with suspected bloodstream infection.","authors":"Fen Pan, Fangyuan Yu, Hong Zhang, Pengcheng Chen, Wenghao Weng","doi":"10.1007/s10096-025-05289-0","DOIUrl":"https://doi.org/10.1007/s10096-025-05289-0","url":null,"abstract":"<p><strong>Background: </strong>Accurate and comprehensive pathogen diagnosis methods are urgently required for the diagnosis of bloodstream infection (BSI).This study retrospectively evaluated the clinical application of metagenomic next-generation sequencing (mNGS) in children with suspected BSI.</p><p><strong>Methods: </strong>Between July 8, 2021 to December 31, 2022, mNGS tests and conventional methods tests (CMTs) were performed simulataneously on samples from children with suspected BSI. The diagnostic performance of mNGS was assessed in comparison CMTs .</p><p><strong>Results: </strong>A total of 191 patients with suspected BSI were included in the final analysis after excluding 9 patients due to lost to follow-up or duplicated entries. The mNGS yielded positive results in 111 cases, with a positive rate of 58.1% (111/191), significantly higher than that of CMTs (13.1%, 25/191) (P < 0.05). Using CMTs as standard, the sensitivity, specificity, positive predictive value and negative predictive value for mNGS and CMTs were 73.8% vs. 25.0%, 54.2% vs. 96.3%, 55.9% vs. 84.0%, and 72.5% vs. 62.0%, respectively. Among 111 mNGS-positive cases, 46 cases (41.4%) showed ploymicrobial infections, with Torque teno virus, human betaherpesvirus 5, and human gammaherpesvirus 4 being most frequently identified pathogens. Of them, 62 cases (55.9%) were clinically diagnosed as BSI regarded as true positive results, while 49 cases (44.1%) positive for pathogens were diagnosed as non-BSI. The diagnostic time of mNGS was significantly shorter than that of CMTs (30.6 ± 7.7 h vs. 70.5 ± 11.6 h, P < 0.05). It is worth noting that mNGS results guided adjustments to antimicrobial therapy in 50.8% (97/191) patients, including escalation in 74 cases and de-escalation in 23 cases.</p><p><strong>Conclusions: </strong>The mNGS significantly improves the detection rate for the pathogens in children with suspected BSI, especially for viruses, which serve as a complement to CMTs.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and simple detection of Chlamydia trachomatis and Neisseria gonorrhoeae using the EasyNAT CT/NG assay based on cross-priming amplification.","authors":"Qing Wu, Hongxiang Tu, Yingjie Dai, Yumin Wang, Lijuan Hu","doi":"10.1007/s10096-025-05218-1","DOIUrl":"10.1007/s10096-025-05218-1","url":null,"abstract":"<p><strong>Purpose: </strong>Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) rank among the most common sexually transmitted pathogens. Rapid screening and detection of these bacteria are essential to reduce sequelae and prevent transmission. This study evaluated the efficacy of the EasyNAT CT/NG assay, which utilizes cross-priming amplification (CPA) technique for the rapid and simultaneous detection of CT and NG in diverse reproductive tract specimens, achieving diagnosis within 30 min.</p><p><strong>Methods: </strong>The clinical performance of the EasyNAT CT/NG assay in detecting CT and NG was assessed using 198 clinical samples, with results compared to those of conventional in-house Real-Time PCR to determine concordance. Sensitivity was measured using serial dilutions of quantified plasmids and specificity was evaluated by incorporating DNA from 18 common STI pathogens. The assay's suitability as a point-of-care testing (POCT) tool was evaluated with the criteria outlined in Target Product Profiles (TPPs).</p><p><strong>Results: </strong>The EasyNAT CT/NG assay demonstrated high concordance with Real-Time PCR, with rates of 98.5% for CT and 99.0% for NG. Concordance in urine samples reached 98.6% for CT and 100% for NG, while cervical swabs showed both 97.7% for CT and NG; vaginal and urethral swabs achieved 100% for both pathogens. Among the 198 samples, one urine specimen tested negative for CT by Real-Time PCR but positive by the EasyNAT CT/NG assay, a positive result confirmed by the Cepheid Xpert CT/NG assay. Two cervical swabs, negative for CT and NG by Real-Time PCR, yielded invalid results with the EasyNAT CT/NG assay but were confirmed negative or CT and NG by the Cepheid Xpert CT/NG assay. The EasyNAT CT/NG assay reliably detected CT and NG in turbid specimens, though it may fail with severely hemolytic samples. Its detection limit was 400 copies/mL, with no cross-reactivity observed across 18 other pathogens.</p><p><strong>Conclusion: </strong>The EasyNAT CT/NG assay offers rapid, sensitive, and specific detection of CT and NG, proving valuable for infection screening and early diagnosis. It shows promise as a rapid POCT method.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2515-2525"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-acquired staphylococcal scalded skin syndrome in an immunocompetent adult.","authors":"Nevio Cimolai","doi":"10.1007/s10096-025-05204-7","DOIUrl":"10.1007/s10096-025-05204-7","url":null,"abstract":"<p><p>Staphylococcal scalded skin syndrome is an exfoliative skin eruption associated with specific exotoxin-producing strains of Staphylococcus aureus. Affliction of adults is considerably rare, and most such patients have pre-disposing co-morbidities. A unique such infection of an immunocompetent adult without risk factors is detailed. Current concepts of disease process and treatment are highlighted for staphylococcal scalded skin syndrome among adults.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2295-2302"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144616884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of carbapenem resistance via MALDI-TOF MS using an innovative broth microgrowth assay.","authors":"Zhenghua Xie, Qun Wang, Ziyuan Zhou, Weixing Wu, Xiaoyu Zhang, Li Zhang","doi":"10.1007/s10096-025-05213-6","DOIUrl":"10.1007/s10096-025-05213-6","url":null,"abstract":"<p><strong>Objectives: </strong>Carbapenem-resistant Enterobacterales (CRE) are a growing threat to human health worldwide. This study aimed to develop a novel method, the broth microgrowth assay, for the rapid identification of CRE from culture isolates using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).</p><p><strong>Methods: </strong>A total of 80 isolates, including carbapenem-resistant Escherichia coli (CRECO, n = 28), carbapenem-sensitive Escherichia coli (CSECO, n = 28), carbapenem-resistant Klebsiella pneumoniae (CRKP, n = 12), and carbapenem-sensitive Klebsiella pneumoniae (CSKP, n = 12) were collected for this study. The minimum inhibitory concentrations (MICs) of imipenem and meropenem were measured for all the isolates via the broth microdilution method. Carbapenem resistance (R) and susceptibility (S) were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing, M100, 34th Edition. These isolates were incubated both with carbapenem antibiotics (imipenem and meropenem) as a detection site and without antibiotics as a growth control site at 35°C for 1 and 2 h, respectively. Following incubation, the mixtures were centrifuged, and the supernatant was pipetted off. The remaining sediment was subsequently applied to the MALDI-TOF MS target plate, and the residual broth was subsequently absorbed via sterile filter paper. Identification of the isolates was performed via the VITEK MS system. The test was deemed valid only if the sample without antibiotics (growth control) resulted in successful species identification (confidence level > 60.0%). Percentage of isolates that successfully grew without antibiotics and were identified by MALDI-TOF MS, which is referred to as the growth efficiency. CRE were distinguished if the microorganisms could be successfully identified.</p><p><strong>Results: </strong>After 1 h of incubation with imipenem or meropenem, the growth efficiencies of E. coli and K. pneumoniae were 83.93% and 66.67%, respectively. For Escherichia coli, the sensitivity and specificity of imipenem resistance prediction by MALDI-TOF MS were 82.14% and 100%, respectively. Conversely, meropenem demonstrated a sensitivity of 89.29% and a specificity of 100%. When Klebsiella pneumoniae was examined, both imipenem and meropenem had sensitivity and specificity values of 83.33% and 100%, respectively. After the incubation time was extended to 2 h, both antibiotics achieved perfect sensitivity and specificity of 100%, coupled with a growth efficiency of 100% for both bacterial strains.</p><p><strong>Conclusion: </strong>Combining the broth microgrowth assay with MALDI-TOF MS offers a rapid and accurate approach to identifying CRE, thus facilitating the swift selection of appropriate antibiotics.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2487-2494"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical features, antimicrobial susceptibilities, treatment characteristics and outcomes of paediatric Acinetobacter lwoffii bacteremia: a case series.","authors":"Ömer Güneş, Aslınur Özkaya-Parlakay, Ahmet Yasin Güney, Zehra Nihan Coşkun, Latife Güder, Özlem Mustafaoğlu, Pinar Bayraktar, Fatih Üçkardeş, Aysun Yahşi, Seval Özen, Tuğba Erat, Belgin Gülhan, Saliha Kanık-Yüksek, Gülsüm İclal Bayhan","doi":"10.1007/s10096-025-05239-w","DOIUrl":"10.1007/s10096-025-05239-w","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the clinical characteristics, antibiotic susceptibilities, therapeutic approaches, and outcomes of paediatric patients with A. lwoffii bacteremia.</p><p><strong>Methods: </strong>A case series was conducted of paediatric patients diagnosed with A. lwoffii bacteremia between September 2019 and December 2022. Demographic data, underlying diseases, sources of infection, antimicrobial susceptibility profiles, treatment modalities, and clinical outcomes were analysed.</p><p><strong>Results: </strong>A total of 12 patients were included, with 58.3% female and a median age of 24 months (IQR: 11-58.5). Overall, 91.7% of isolates were susceptible to amikacin, 83.3% to ceftazidime, 83.3% to colistin, 66.6% to meropenem, 66.6% to piperacillin-tazobactam, and 100% to tigecycline. Three patients received combination antibiotic therapy and nine patients received monotherapy. Antibiotic lock therapy (ALT) was successfully applied in one patient with catheter-related bloodstream infection (CRBSI) whose central venous catheter (CVC) could not be removed. The median infection-related length of hospital stay was 10 days (IQR: 10-14 days). The median duration of bacteremia was 2 days (IQR: 2-3). No cases of persistent bacteremia, recurrence, or infection-related mortality were observed. Susceptibility-guided monotherapy or combination therapy resulted in favourable outcomes in all cases, underscoring the importance of pathogen-directed treatment.</p><p><strong>Conclusion: </strong>Acinetobacter lwoffii remains an important pathogen of hospital-acquired bacteraemia in hospitalised children. Treatment with broad-spectrum beta-lactam antibiotics, either as monotherapy or combined with amikacin or colistin, appears effective.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2539-2548"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of isolation and drug testing methods for Corynebacterium spp. in clinical specimens of granulomatous mastitis.","authors":"Fang Liu, Yewei Yuan, Ruiyang Wu, Ninghua Tang, Kehua Chen, Kui Fan, Wei Cheng, Qu Pan","doi":"10.1007/s10096-025-05220-7","DOIUrl":"10.1007/s10096-025-05220-7","url":null,"abstract":"","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2527-2537"},"PeriodicalIF":3.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}