Comparison of Meltpro Myco Assay with MALDI-TOF Mass Spectrometry for rapid and accurate detection of clinical Mycobacterium Species.

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2025-05-26 DOI:10.1007/s10096-025-05171-z

Kehao Peng, Liuyue Xu, Xuezhi Wang, Jianming Hong, Gengchen Zhang, Yuchuan Zhao, Yinong Ye, Zengqi Huang, Jinmei Zhang, Yuchun Huang, Zhiqiao Wu, Xiaolin Yu, Yanqin Song, Shihao Chen, Yongping Wang, Yuhui Chen, Huizhong Wu, Xiaoyu Lai, Meiling Yu, Jingjing Liang, Wenjing Wei

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引用次数: 0

Abstract

Purpose: The aim of this study was to compare the performance of the MeltPro Myco assay with that of MALDI-TOF mass spectrometry for detecting nontuberculous mycobacteria (NTMs) from clinical isolates and samples.

Method: A total of 308 suspected NTM isolates from sputum or BALF samples of patients with pulmonary diseases were retrospectively analyzed. DNA sequencing was used as the gold standard method. An additional 164 clinical samples from patients with pulmonary diseases were used to assess the suitability of the MeltPro Myco assay for clinical use.

Result: The results indicated that for NTM identification, the MeltPro Myco assay and MALDI-TOF had sensitivities of 99.3% and 97.2% and specificities of 90.9% and 100%.The MeltPro Myco assay achievies a sensitivity and specificity of 79.4% and 95.2% for the detection of NTM in clinical samples.

Conclusion: Given its rapidity, straightforward operation, high accuracy, cost-effectiveness, and good reproducibility, the MeltPro Myco assay can be a valuable tool for timely screening of lung disease caused by NTM and has the potential to improve the diagnosis and prevention of NTM pulmonary disease in highly endemic areas.

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Meltpro Myco法与MALDI-TOF质谱法快速准确检测临床分枝杆菌的比较

目的：本研究的目的是比较MeltPro Myco法与MALDI-TOF质谱法检测临床分离株和样品中非结核分枝杆菌（ntm）的性能。方法：对308例肺部疾病患者痰液或BALF标本中疑似NTM分离株进行回顾性分析。采用DNA测序作为金标准方法。另外164例肺部疾病患者的临床样本被用于评估MeltPro Myco检测在临床应用中的适用性。结果：MeltPro Myco法和MALDI-TOF法鉴定NTM的灵敏度分别为99.3%和97.2%，特异性分别为90.9%和100%。MeltPro Myco检测临床样品中NTM的灵敏度和特异性分别为79.4%和95.2%。结论：MeltPro Myco检测具有快速、简便、准确性高、成本效益好、重复性好等优点，可作为及时筛查NTM肺部疾病的重要工具，在NTM高流行地区提高对NTM肺部疾病的诊断和预防水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.