European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Evaluating the clinical impact of targeting lower versus higher serum vancomycin trough: a retrospective study using a desirability of outcome ranking (DOOR) analysis.","authors":"Zong Heng Shi, Sean W X Ong, Lesley Palmay, Nathan H Ma, Marie-Félixe Granger, Philip W Lam, Marion Elligsen","doi":"10.1007/s10096-025-05161-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05161-1","url":null,"abstract":"<p><strong>Purpose: </strong>Guidelines recommend a target AUC₂₄/MIC of 400-600 for vancomycin dosing in serious MRSA infections. However, various challenges hinder the implementation of AUC-based monitoring. Previously recommended target trough of 15-20 mg/L may be associated with increased nephrotoxicity.</p><p><strong>Methods: </strong>In this single-center, retrospective study, we compared outcomes of targeting troughs of 10-15 mg/L vs 15-20 mg/L for intermittent infusion of vancomycin (IIV) in adult hospitalized patients who received ≥ 48 h of IIV and had ≥ 1 trough measurement within recommended ranges. Local guidelines recommended target troughs of 15-20 mg/L between January 2019 and December 2020 and 10-15 mg/L between January 2022 and October 2023. Treatment failure, acute kidney injury (AKI) and inpatient mortality were compared using a desirability of outcome ranking (DOOR) analysis between the two time periods. The most desirable outcome was treatment success and no AKI, and the least desirable outcome was death.</p><p><strong>Results: </strong>A total of 173 IIV courses were included. The probability of obtaining a better DOOR was 57.9% (95%CI 50.5-64.9, p = 0.03) when targeting a lower trough. Secondary analyses demonstrated a similar trend. When analyzed separately, the lower target trough group experienced significantly less AKI (OR 0.40, 95%CI 0.16-0.96) with no significant effect on mortality and treatment failure.</p><p><strong>Conclusion: </strong>Our study showed that targeting a trough of 10-15 mg/L for IIV may be associated with a superior overall outcome compared to targeting 15-20 mg/L. In centers not using AUC-based monitoring for vancomycin dosing, a lower target trough may be preferable.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a colorimetric loop-mediated isothermal amplification assay for Helicobacter pylori detection.","authors":"Chonticha Romyasamit, Apichat Kaewdech, Pimsiri Sripongpun, Naichaya Chamroonkul, Maseetoh Samaeng, Dechawat Wongprot, Phanvasri Saengsuwan, Morteza Saki, Phoomjai Sornsenee","doi":"10.1007/s10096-025-05153-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05153-1","url":null,"abstract":"<p><strong>Purpose: </strong>Helicobacter pylori is an important pathogen responsible for various gastrointestinal disorders, including peptic ulcers and gastric cancer. Rapid and accurate detection of H. pylori infection is crucial for its early diagnosis and treatment. This study aimed to develop and evaluate the efficacy of a colorimetric loop-mediated isothermal amplification (C-LAMP) assay for the detection of H. pylori in tissue biopsy samples.</p><p><strong>Methods: </strong>In total, 302 gastric biopsy samples were collected, and the performance of the C-LAMP assay was compared with that of conventional diagnostic methods, including culture, PCR, and rapid urease test (CLO test).</p><p><strong>Results: </strong>The detection limit of the C-LAMP assay was 1 CFU/mL with a rapid reaction time of 15 min at 61 °C, highlighting its efficiency for rapid diagnosis. Compared to culture, the assay demonstrated a sensitivity of 80% and specificity of 98%, whereas compared to PCR, sensitivity was 60% and specificity was 100%. ROC analysis revealed superior diagnostic accuracy of the C-LAMP assay (AUC = 0.80 using PCR as reference; AUC = 0.89 using culture as reference) relative to the CLO test (AUC = 0.63 vs. PCR; AUC = 0.65 vs. culture), culture (AUC = 0.60 vs. PCR), and PCR (AUC = 0.78 vs. culture).</p><p><strong>Conclusion: </strong>These results suggest that the C-LAMP assay is a highly sensitive, specific, and cost-effective tool for rapid detection of H. pylori, offering significant advantages over conventional diagnostic methods, particularly in resource-limited settings, and the C-LAMP assay is a promising alternative for early and reliable H. pylori detection in both clinical and field settings.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The emergence of highly pathogenic avian influenza H5N1 in dairy cattle: implications for public health, animal health, and pandemic preparedness.","authors":"Mohamed Kamel, Sami Aleya, Wesam Taher Almagharbeh, Lotfi Aleya, Mohamed M Abdel-Daim","doi":"10.1007/s10096-025-05147-z","DOIUrl":"https://doi.org/10.1007/s10096-025-05147-z","url":null,"abstract":"<p><strong>Background: </strong>The 2024 outbreak of Highly Pathogenic Avian Influenza (HPAI) H5N1 in U.S. dairy cattle represents a significant change in the behavior of zoonotic influenza viruses. Previously, H5N1 was primarily an avian pathogen with limited infection in mammals. The emergence of clade 2.3.4.4b H5N1 in dairy herds across multiple states reveals the virus's adaptation to mammalian hosts and highlights its potential for zoonotic transmission, raising important concerns for public health, veterinary medicine, and agriculture.</p><p><strong>Results: </strong>The virus demonstrated unique tropism for mammary tissue, with high viral loads detected in milk. Genomic analysis identified mutations that enhance binding to mammalian receptors and facilitate systemic spread. Viral RNA was found in raw milk, posing food safety risks; however, standard pasteurization effectively inactivated the virus. Epidemiological data indicate the outbreak likely began with spillover from wild birds or contaminated fomites, followed by efficient local transmission within herds. Forty-one human cases linked to infected dairy operations were confirmed. The outbreak caused significant economic losses due to decreased milk production and trade restrictions. Although human-to-human transmission remains low, the zoonotic risk requires urgent attention.</p><p><strong>Conclusion: </strong>The 2024 HPAI H5N1 outbreak in U.S. dairy cattle highlights critical gaps in surveillance, biosecurity, and coordination across sectors. A One Health approach integrating veterinary, public health, and environmental efforts is essential. Recommendations include improved surveillance, stringent biosecurity measures, occupational safety protocols, focused research on viral evolution, and investment in diagnostics and vaccines. These actions are vital to reduce risks, protect public health, and ensure the sustainability of the dairy industry against future zoonotic influenza threats.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between clinical outcome and microbiological findings in peritonsillar abscess - an observational study.","authors":"Elin Lindberg, Ann Hermansson, David Nygren, Marie Gisselsson-Solén","doi":"10.1007/s10096-025-05156-y","DOIUrl":"https://doi.org/10.1007/s10096-025-05156-y","url":null,"abstract":"<p><strong>Background: </strong>Previous studies on causative pathogens in peritonsillar abscesses have yielded varying results. However, Group A streptococci (GAS) and Fusobacterium necrophorum have been identified frequently. The aim of this study was to investigate pathogens in peritonsillar abscesses in patients tested for both ß-hemolytic streptococci and F. necrophorum, and to investigate associations between pathogens and clinical outcome.</p><p><strong>Method: </strong>This retrospective observational study included all patients in the Skåne Region, Sweden (population 1.4 million) with a diagnosis of peritonsillar abscess between 2016 and 20, and in whom tests were performed for both ß-hemolytic streptococci (culture) and F. necrophorum (PCR). Exclusion criteria included previous (30 days) purulent complication to pharyngotonsillitis or antibiotic therapy. Chart review from 30 days prior to 6 months after the index visit was performed. Logistic regression was performed to evaluate the association between pathogens and complications, with negative microbiological findings set as reference category. Complications were defined as a composite outcome (0/1) of recurrent pharyngotonsillitis/peritonsillar abscess, other pharyngeal abscess or septic complications within 30 days (early), and 1-6 months (late).</p><p><strong>Results: </strong>In a total of 637 patients, F. necrophorum was identified in 210 (33%), GAS in 159 (28%) and GCS/GGS in 40 (6%) patients. F. necrophorum was most common in adolescents and young adults. Only F. necrophorum was associated with the development of either of early (OR 3.8 (2.0-7.1 95%CI)) and late complications (OR 2.5 (95%CI 1.3-4.9).</p><p><strong>Conclusion: </strong>F. necrophorum was the most commonly identified pathogen in peritonsillar abscesses. It was also the only pathogen associated with the development of complications.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Mycobacterium europaeum from a clinical sample in China: report of genome sequence and literature review.","authors":"Feifei Zhao, Dan Zhou, Yu Feng, Yi Xie, Zhiyong Zong","doi":"10.1007/s10096-025-05159-9","DOIUrl":"https://doi.org/10.1007/s10096-025-05159-9","url":null,"abstract":"<p><p>Mycobacterium europaeum, of Mycobacterium simiae complex, is a rare nontuberculous mycobacterium associated with human diseases. We report a case with an isolate from bronchoalveolar lavage fluid and summarize M. europaeum cases from literature. M. europaeum may cause respiratory infections and colonization in immunocompromised, elderly, and/or chronic pulmonary illness patients.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genetic mutations conferring tedizolid resistance in MRSA mutants.","authors":"Nesma B Goda, Amira M El-Ganiny, Tharwat R El-Khamissy, Fares Z Najar, Ashraf A Kadry","doi":"10.1007/s10096-025-05157-x","DOIUrl":"https://doi.org/10.1007/s10096-025-05157-x","url":null,"abstract":"<p><strong>Purpose: </strong>In light of previous studies eliminating the involvement of gene-mediated mechanisms in developing tedizolid resistance, our study elucidates the ability of mutation-mediated mechanisms to confer oxazolidinones cross-resistance in methicillin-resistant Staphylococcus aureus (MRSA). With further investigation of the identified mutations and their relation to tedizolid resistance. Additionally, the involvement of rpoB mutations in acquiring resistance to tedizolid was also investigated.</p><p><strong>Methods: </strong>Five cfr-negative, methicillin-resistant Staphylococcus aureus clinical isolates were subjected to in vitro selection to develop linezolid-resistant mutants. The resultant mutants were tested for acquiring tedizolid cross-resistance, whole genome sequencing was performed twice, followed by variant calling and annotation. Detected mutations were analyzed for their relatedness to the developed resistance.</p><p><strong>Results: </strong>Mutations considered relevant to tedizolid resistance were detected in rpoB gene encoding β-subunit of the RNA polymerase enzyme and rplC gene encoding the 50S ribosomal protein L3. Additionally, mutations in mepB gene, part of the mepRAB operon were detected and believed to contribute to acquiring linezolid resistance.</p><p><strong>Conclusion: </strong>To the best of our knowledge, our findings are the first to report the 50S ribosomal protein L3 mutation Gly152Asp to solely confer cross-resistance to both linezolid and tedizolid oxazolidinones. In addition, we report the emergence of cross-resistance between oxazolidinone antibiotics and rifampin through a single amino-acid substitution occurring within the Rifampin Resistance Determining Region (RRDR). Furthermore, mepB mutations reported in our results support a theory implying a second MepR-independent mechanism regulating the mepRAB operon, and are believed to be responsible for the acquired linezolid resistance in our study.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nationwide multicentre study of Nanopore long-read sequencing for 16S rRNA-species identification.","authors":"Sofia Brunet, Anna Grankvist, Daniel Jaen-Luchoro, Maria Bergdahl, Jean-Luc Tison, Annica Wester, Karin Elfving, Jule Brandenburg, Karolina Gullsby, Christoffer Lindsten, Lars-Ola Arvidsson, Helena Larsson, Hinnerk Eilers, Anna Söderlund Strand, Mimi Lannefors, Johanna Keskitalo, Felicia Rylander, Jenny Welander, Malin Bergman Jungestrom, Miriam Geörg, Rene Kaden, Ida Karlsson, Anna-Malin Linde, Sara Mernelius, Linda Berglind, Lars Feuk, Susanne Kerje, Linda Karlsson, Andreas Sjödin, Lina Guerra-Blomqvist, Frans Wallin, Anna Fagerström, Martin Vondracek, Paula Mölling, Erika Tång Hallbäck","doi":"10.1007/s10096-025-05158-w","DOIUrl":"https://doi.org/10.1007/s10096-025-05158-w","url":null,"abstract":"<p><strong>Purpose: </strong>Recent improvements in Nanopore sequencing chemistry has made it a promising platform for long-read 16S rRNA sequencing. This study evaluated its clinical utility in a nationwide collaboration coordinated by Genomic Medicine Sweden.</p><p><strong>Methods: </strong>Thirteen mock samples comprised of various bacterial strains and an External Quality Assessment (EQA) panel from QCMD (Quality Control for Molecular Diagnostics) were analysed by 20 microbiological laboratories across Sweden, using the recent v14 chemistry. Most laboratories generated full-length 16S rRNA sequencing libraries using an optimized protocol for the 16S Barcoding Kit 24, while two laboratories employed in-house PCR coupled with the Ligation Sequencing Kit. The commercial 16S bioinformatic pipeline from 1928 Diagnostics (1928-16S) was evaluated and compared with the open-sourced gms_16S pipeline that is based on the EMU classification tool (GMS-16S).</p><p><strong>Results: </strong>Seventeen out of 20 laboratories successfully sequenced and analysed the samples. Laboratories that used sodium acetate-containing elution buffers faced compatibility issues during library construction, resulting in reduced read count. High bacterial load samples were generally well-characterized, whereas hard-to-lyse bacteria such as Gram-positive strains were detected at lower abundance. The GMS-16S tool provided improved species-level identification compared to the 1928-16S pipeline, particularly for closely related taxa within the Streptococcus and Staphylococcus genera.</p><p><strong>Conclusion: </strong>Nanopore sequencing demonstrated promising potential for bacterial identification in a clinical setting. The results prompt further optimization of the protocol to improve detection of a broader range of species. This multicentre study highlights the feasibility of implementing Nanopore sequencing into clinical microbiological laboratories, for improved national precision diagnostics.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and validation of a predictive model for the risk of multidrug-resistant Klebsiella pneumoniae infection based on machine learning algorithms - a multicenter retrospective study.","authors":"Tao Sun, Pei-Pei Wang, Jun-Ji Liu, Zhen An, Jun-Rong Zhao, Jun Liu","doi":"10.1007/s10096-025-05152-2","DOIUrl":"https://doi.org/10.1007/s10096-025-05152-2","url":null,"abstract":"<p><strong>Objective: </strong>The development of a reliable predictive model for Multi-drug-resistant Klebsiella pneumoniae (MDR-KP) infections is imperative for the timely identification of at-risk individuals.</p><p><strong>Methods: </strong>This study analyzed data from 3,554 patients with KP infection at multiple hospitals. By comparing six machine learning algorithms (Logistic Regression(LR), Efficient Neural Network (ENet), Decision Tree(DT), MultiLayer Perceptron(MLP), Support Vector Machine(SVM), and Extreme Gradient Boosting(XGBoost)), we constructed and validated the prediction model. Furthermore, the model interpretation was conducted through SHapley Additive exPlanations (SHAP) analysis. Subsequently, nomograms were developed to estimate the risk of MDR-KP infection in hospitalized individuals. Finally, the association between independent variables and the risk of MDR-KP acquisition was elucidated through Restricted Cubic Spline (RCS) analysis.</p><p><strong>Results: </strong>The results of the multivariable logistic regression analysis indicated that C-reactive protein (CRP), Uric Acid (UA), Urea, Platelet (PLT), Hemoglobin (HB), Red blood cell counts (RBC), Age, and Gender were identified as independent risk factors. We incorporated these independent risk factors into six machine learning, and found that the XGBoost-based model exhibited superior performance compared to other machine learning algorithms, achieving a recall of 0.732, an F1 score of 0.707, and an AUC of 0.777. Furthermore, the SHAP method highlighted Urea, UA, and PLT as the primary decision factors predicted by the machine learning model, and the RCS analysis revealed a nonlinear relationship between Age, CRP, RBC, HB, UA, UREA, and the risk of MDR-KP infection.</p><p><strong>Conclusion: </strong>This study has developed an effective risk prediction model for MDR-KP infection. The model has the potential to assist healthcare providers in early identification of high-risk patients, enabling timely implementation of preventive and therapeutic interventions.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and outcomes of tuberculosis and non-tuberculous mycobacteria infections: a population-based study of concurrent and sequential infections.","authors":"Zhen-Tao Fei, Dan-Ping Zhou, Wei Huang, Ping Liu, Qian-Ping Lu, Hao Li, Yang Yang, Dan Ye, Xu-Hui Liu, Lu Xia","doi":"10.1007/s10096-025-05151-3","DOIUrl":"https://doi.org/10.1007/s10096-025-05151-3","url":null,"abstract":"<p><strong>Background: </strong>Co-infection with Tuberculosis (TB) and non-tuberculous mycobacteria (NTM) poses significant diagnostic and therapeutic challenges. This study investigates the demographic, clinical, and survival characteristics of these patients.</p><p><strong>Methods: </strong>This retrospective study included patients diagnosed with TB, NTM, or TB + NTM co-infection at the Shanghai Public Health Clinical Center (2019-2022). Clinical, imaging, and survival data were collected and analyzed.</p><p><strong>Results: </strong>A total of 400 patients were included: 33 in the TB + NTM group, 167 in the NTM-only group, and 200 in the TB-only group. Chest pain was more common in the TB + NTM group than the TB-only group (P = 0.006). The TB + NTM group exhibited a significantly lower body mass index (19.95 ± 3.51) and serum albumin level (36.09 ± 4.93 g/l), as well as a higher prevalence of hilar lymphadenopathy (45.5%) and cavitary lesions (39.4%), compared to the TB-only or NTM-only groups (P < 0.05). Miliary nodules were more frequent in the TB + NTM group (24.2%) compared to the TB-only group (11.0%, P = 0.048). A higher proportion of TB + NTM patients were from Central and Western China (P = 0.005). Survival analysis showed worse outcomes for the TB + NTM group (P = 0.038 vs. NTM-only, P = 0.008 vs. TB-only). Cox regression further identified a higher mortality risk in the TB + NTM group (adjusted hazard ratios = 2.468, 95% CI: 1.079-5.642, P = 0.032).</p><p><strong>Conclusion: </strong>TB + NTM co-infection is associated with distinct clinical features and worse survival outcomes, emphasizing the need for early diagnosis and tailored treatment.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granulicatella adiacens infections in children: a single-center retrospective study.","authors":"Türkan Aydın Teke, Ayşe Kaman, Fatma Nur Öz, Zeynep Gökçe Gayretli Aydın, Hülya Şeker Yıkmaz, Gülsüm İclal Bayhan","doi":"10.1007/s10096-025-05154-0","DOIUrl":"https://doi.org/10.1007/s10096-025-05154-0","url":null,"abstract":"<p><strong>Purpose: </strong>Granulicatella spp. although rare, are notable pathogens in various infections, particularly in bacteraemia and infective endocarditis. Because of their unique growth requirements and difficulties in antimicrobial testing, identifying and treating these microorganisms poses significant challenges.</p><p><strong>Methods: </strong>This retrospective study was conducted at Dr. Sami Ulus Maternity and Children's Health and Diseases Research and Education Hospital, a tertiary care centre in Ankara, Turkey. Blood cultures were screened for Granulicatella spp. between January 2005 and January 2017. Clinical and laboratory features of the patients were documented.</p><p><strong>Results: </strong>During the 12-year study period, 4125 patients with positive blood culture results were investigated. No cases of G. para-adiacens or G. elegans infection were identified. G. adiacens infection was diagnosed in seven patients (five males and two females) representing 0.1%. The mean age of the patients was 79.5 ± 49.8 months (median: 96 months, range: 10-140 months). Six patients had underlying conditions, including congenital heart diseases (two patients), gastrointestinal diseases (two patients), haematological malignancy (one patient), and neurological disorders (one patient). Three patients had bacteraemia, two had central line-related bloodstream infection (CRBSI), one had bacteraemia and pneumonia, and one had infective endocarditis. Four infections were community-acquired and three were healthcare-associated. All patients survived.</p><p><strong>Conclusion: </strong>Although rare, G. adiacens can cause severe infections in children. Clinicians should be particularly vigilant for this pathogen, especially in children with cardiac disease, malignancy, or mucosal disruption, particularly when slow-growing gram-positive cocci are isolated from blood cultures or other sterile sites.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}