European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Clinical impact and risk factors of enterococcal bacteremia in children: a focus on vancomycin resistance and mortality.","authors":"Büşra Demirci, Belgin Gülhan, Sevinç Püren Yücel, Fehmiye Funda Karkın Gürel, Bengü Hakbilen, Büşra Elif Şahin Gölge, Saliha Kanık-Yüksek, Tuğba Erat, Aysun Yahşi, Seval Özen, Fatma Burçin Kurtipek, Gülsüm İclal Bayhan, Aslınur Özkaya-Parlakay","doi":"10.1007/s10096-025-05263-w","DOIUrl":"https://doi.org/10.1007/s10096-025-05263-w","url":null,"abstract":"<p><strong>Background: </strong>Enterococcal bloodstream infections (EBIs) are increasingly concerning in pediatric patients due to high morbidity, mortality, and antimicrobial resistance, especially vancomycin-resistant enterococci (VRE). This study aimed to evaluate clinical characteristics, species distribution, risk factors, and outcomes of pediatric EBSIs, focusing on VRE and mortality predictors.</p><p><strong>Methods: </strong>This retrospective study analyzed 368 children with confirmed enterococcal bacteremia. Demographic, clinical, microbiological, and treatment data were collected. Comparative analyses were done between species, VRE vs. vancomycin-susceptible enterococci (VSE), and mortality outcomes. Logistic regression identified independent risk factors for VRE and mortality.</p><p><strong>Results: </strong>Mean age was 5.2 ± 3.8 years, with 53.8% male. Common underlying conditions included hematologic/oncologic malignancies (20.9%), necrotizing enterocolitis (18.8%), and neurological disorders (16.8%). E. faecalis (51.9%) and E. faecium (41.6%) were the most frequent isolates, with VRE detected in 10.9%, predominantly among E. faecium isolates. Older age, prior VRE colonization, E. faecium infection, total parenteral nutrition (TPN) use, and persistent bacteremia significantly increased the risk of VRE infection. The overall 30-day mortality rate was 9.0%, while the total mortality during follow-up reached 20.7%. Thirty-day mortality was independently predicted by prior carbapenem use (OR = 3.2), whereas overall mortality was significantly associated with receipt of TPN (OR = 1.9), ICU admission (OR = 6.5), persistent bacteremia (OR = 3.6), and prior vancomycin use (OR = 2.2).</p><p><strong>Conclusion: </strong>Pediatric EBSIs are linked with significant comorbidities and mortality. VRE presence, persistent infection, and prior broad-spectrum antibiotic use increase mortality risk, highlighting the need for antimicrobial stewardship and risk-based management.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of risk factors for otitis media in hospitalized children aged ≤ 12 years with bacterial pneumonia.","authors":"Liyuan Han, Jie Liu, Yanhua Li, Minglei Liu, Huimiao Liu, Jinghua Wu, Chunyan Gao","doi":"10.1007/s10096-025-05264-9","DOIUrl":"https://doi.org/10.1007/s10096-025-05264-9","url":null,"abstract":"<p><strong>Background: </strong>To analyze the risk factors for otitis media in children aged ≤ 12 years who develop the condition after being hospitalized for bacterial pneumonia.</p><p><strong>Methods: </strong>A total of 411 hospitalized patients aged ≤ 12 years with bacterial pneumonia, admitted between November 2023 and March 2025, were included. Demographic data, birth history, feeding history, and laboratory indicators were recorded to assess their connection with the development of otitis media after hospitalization. First, univariate logistic regression was performed, followed by multivariate logistic regression using a backward stepwise method to establish the final predictive model. The model's effectiveness was assessed based on the AUC (area under the ROC curve), calibration plots, and decision curve analysis (DCA).</p><p><strong>Results: </strong>Patients were stratified into two groups based on the occurrence of otitis media: the non-otitis media group (n = 303) and the otitis media group (n = 108). The study found that several factors were significantly associated with the risk of developing otitis media in children hospitalized with bacterial pneumonia: age 3-12 years (OR: 0.51, 95%CI: 0.26-0.97, p = 0.012), infection with Haemophilus influenzae (HI) (OR: 1.91, 95%CI: 1.07-3.42, p = 0.027), antibiotic use prior to admission (OR: 2.54, 95%CI: 1.29-5.27, p = 0.009), platelet count (OR: 1.00, 95%CI: 1.00- 1.01, p = 0.002), hemoglobin level (OR: 0.95, 95%CI: 0.93-0.98, p < 0.001), alanine aminotransferase (ALT) (OR: 1.04, 95%CI: 1.01-1.07, p = 0.019), creatine kinase (CK) (OR: 1.02, 95%CI: 1.01-1.04, p < 0.001), lactate dehydrogenase (OR: 1.00, 95%CI: 1.00-1.01, p = 0.049). The final model showed that age ≤ 3 years, HI infection, take antibiotics, higher monocyte count, higher PLT, lower HGB, higher ALT, higher CK and LDH were risk factors for OM.</p><p><strong>Conclusion: </strong>The results emphasize the role of demographic characteristics, birth history, feeding history, and laboratory indicators in the risk of developing otitis media during hospitalization in children aged ≤ 12 years with bacterial pneumonia. Incorporating these risk factors into clinical practice may enhance early identification, guide targeted prevention strategies, and ultimately improve outcomes in high-risk populations.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New evaluation of the Toxoplasma ELITe MGB assay on the ELITe Ingenius™ platform (ELITechGroup).","authors":"Marie-Pierre Brenier-Pinchart, Cécile Garnaud, Gaelle Dewaele, Florent Saragosa, Heidi Primard, Marie Gladys Robert, Hervé Pelloux","doi":"10.1007/s10096-025-05265-8","DOIUrl":"https://doi.org/10.1007/s10096-025-05265-8","url":null,"abstract":"","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Care for peripheral intravenous catheter: an audit in two French university hospitals.","authors":"Sophie Gardes, Alexandra Pandini, Karim Tazarourte, Céline Liard, Florian Deschamps, Pauline Verbist, Stephane Nancey, Eddy Cotte, Véronique Potinet, Philippe Vanhems, Nagham Khanafer","doi":"10.1007/s10096-025-05243-0","DOIUrl":"https://doi.org/10.1007/s10096-025-05243-0","url":null,"abstract":"<p><p>To assess adherence to French guidelines for the prevention of complications, particularly infections, associated with peripheral intravenous catheters (PIVC). An observational audit was conducted across seven wards in two university hospitals. A digital tool was used to capture real-time compliance data based on the recommendations of the French Society of Hospital Hygiene. Data were collected among nurses caring PIVC over two periods: July 9-19, 2021, and November 22-26, 2021. Observations were carried out only during weekday daytime hours. A total of 120 insertions, maintenance, or removal were recorded. The compliance with key safety measures varied widely. Healthcare workers (HCWs) verified patient identity in only 50% of cases. Hand hygiene compliance rates were 82.5% before catheter insertion, 54% before infusion preparation or administration and dropped to just 13% before catheter removal.Catheter flushing was performed in 82.5% of insertions and 66% of post-infusion procedures; however occlusion was the common reason for catheter removal (14.3%). Glove use during insertion was observed in 47% of the cases.The audit highlighted inconsistent adherence to national recommendations for PIVC care. While some practices seem well integrated, others are insufficiently applied. These findings underscore the need to investigate barriers to adherence to evidence-based guidelines.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Phoenix sepsis score: characterization of a cohort of children of different primary statuses and ages, 2020-2024.","authors":"Wanyu Jia, Ruiyang Sun, Jiapu Hou, Zewen Ding, Peng Li, Chunlan Song","doi":"10.1007/s10096-025-05255-w","DOIUrl":"https://doi.org/10.1007/s10096-025-05255-w","url":null,"abstract":"<p><p>The purpose was to validate the applicability of the Phoenix Sepsis Score and to compare it with other scores. The applicability of the Phoenix Sepsis Score in children with different primary statuses and age groups was compared. This study is a retrospective study. Children aged between 1 month and 18 years with a clinically suspected diagnosis of sepsis from January 2020 to December 2024 were selected for the study. Clinical data were collected and Phoenix Sepsis Score, paediatric Sequential Organ Failure Assessment, and Pediatric Logistic Organ Dysfunction Score-2 scores were calculated. The predictive values were assessed using the receiver operator characteristic curve. Compare the area under the curve of different receiver operator characteristic curves. A total of 341 children were included, of which 203 were Male, with a median age of 32 months. There were 103 children with primary diseases. Based on the Phoenix Sepsis Score, 174patients were diagnosed with sepsis. All three scores were significantly higher in the death group. The areas under the curve of the Phoenix Sepsis Score, paediatric Sequential Organ Failure Assessment and Pediatric Logistic Organ Dysfunction Score-2 scores for death in children with suspected sepsis were 0.874(0.834,0.907), 0.884(0.846,0.923) and 0.867(0.826,0.901), respectively. There were no statistically significant differences between the three scores in terms of predictive value. The area under the curve of Phoenix Sepsis Score for death in all age groups from 1 month to 11 years old were greater than 0.808(0.700,0.890). The areas under the curve of the Phoenix Sepsis Score for death in children with or without primary diseases were 0.790(0.699,0.864) and 0.894(0.839,0.949), respectively. The Phoenix Sepsis Score, paediatric Sequential Organ Failure Assessment and Pediatric Logistic Organ Dysfunction Score-2 scores had high predictive value for mortality in children with suspected sepsis. The Phoenix Sepsis Score had good predictive value in children at different primary states and different age. The predictive value of the Phoenix Sepsis Score was lower in children with primary disease.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Historical evolution of the epidemiology of Malaria in Spain and Europe. Future perspectives.","authors":"Ignacio Marañés Antoñanzas, Yolanda López Gallardo, Ángela García de Oro, Carmen Gutiérrez González, Ramón Peyró Sánchez, Sandra Sánchez Martínez","doi":"10.1007/s10096-025-05246-x","DOIUrl":"https://doi.org/10.1007/s10096-025-05246-x","url":null,"abstract":"<p><strong>Background: </strong>Malaria is a disease caused by a parasite of the Plasmodium species transmitted by anopheline mosquitoes that was officially eradicated in Spain in 1964 and in Europe in the 1970s through a combination of implementation of health programs with drug therapy, insecticide spraying and environmental engineering. Since then, the disease has been imported to the continent by international travelers and immigrants from endemic regions.</p><p><strong>Methods: </strong>Diverse medical articles in English and Spanish language, guides and official reports from International Health Organisms were selected, as well as the official WHO (World Health Organization) website (accessing its reports for the years 2023 and 2024) and ECDC (European Centre for Disease Prevention and Control) website with its annual epidemiological reports. The searches were clearly focused on providing answers to the main objective, which is to offer a historical, past and current description of epidemiology in Spain and Europe, and as secondary objectives, to consider how migratory and population movements, as well as climate change, may influence a new disease scenario and how we can anticipate the problems that will arise from this situations.</p><p><strong>Results: </strong>Nowadays there are a high number of imported cases, with autochthonous transmission in Europe being extremely infrequent, probably because of early diagnosis and treatment enabled by efficient healthcare systems. It is speculated that the current changing climatic conditions with warm temperatures and adequate humidity could favor the proliferation of anopheline mosquitoes and thus malaria transmission in several areas of southern Europe. In addition, the continuing massive influx of refugees and migrants from endemic areas could contribute to the creation of a reservoir of infectious parasites.</p><p><strong>Conclusion: </strong>Although the disease potential in Europe is currently low, especially in the northern and western parts of the Continent, strengthening disease awareness and maintaining strong public health infrastructures for surveillance and vector control are of utmost importance to avoid the possibility of malaria transmission and re-emergence in the most vulnerable areas of Europe.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct codon usage signatures reflecting evolutionary and pathogenic adaptation in the Acinetobacter baumannii complex.","authors":"Ujwal Dahal, Anuj Sharma, Karan Paul, Anu Bansal, Shelly Gupta","doi":"10.1007/s10096-025-05233-2","DOIUrl":"https://doi.org/10.1007/s10096-025-05233-2","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigates codon usage and amino acid usage bias in the genus Acinetobacter to uncover the evolutionary forces shaping these patterns and their implications for pathogenicity and biotechnology.</p><p><strong>Methods: </strong>Codon usage patterns were examined in representative genomes of the genus Acinetobacter using standard codon bias indices, including GC content, relative synonymous codon usage (RSCU), effective number of codons (ENC), and codon adaptation index (CAI). Neutrality and parity plots were employed to evaluate the relative influence of mutational pressure and natural selection on codon preferences. Codon pair usage and amino acid composition were assessed to identify functional constraints, while phylogenetic relationships were inferred using gyrB gene sequences to explore evolutionary divergence in the genus Acinetobacter.</p><p><strong>Results: </strong>A dynamic GC content range was observed, from 35.71% in Acinetobacter equi to 46.21% in Acinetobacter indicus, with the Acinetobacter baumannii complex. maintaining a balanced GC distribution (39%), suggesting genomic stability. AT-rich preferred codons indicated selective pressure favouring specific codons, while correlations with GC composition underscored the role of mutational bias. CAI values >0.5 in highly expressed genes denote bias toward optimal codons. Codon pair usage revealed distinctive patterns, with notable similarities within the Acinetobacter baumannii complex, indicating functional congruence. The gyrB phylogeny clustered Acinetobacter baumannii complex despite evolutionary divergence.</p><p><strong>Conclusion: </strong>Codon usage in the genus Acinetobacter reflects a complex interplay of mutational and selective forces, shaping genomic and functional diversity. Distinct disparities in codon usage between pathogenic and non-pathogenic species point to genomic signatures that may underpin virulence, warranting further investigation into their pathogenic potential.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From neglected to notoriety: a review of Mpox clinical features, virology, epidemiology, treatment and prevention strategies.","authors":"Clément Viguier, Pierre Delobel, François-Xavier Lescure, Simon Bessis, Jean-Michel Mansuy, Guillaume Martin-Blondel","doi":"10.1007/s10096-025-05242-1","DOIUrl":"https://doi.org/10.1007/s10096-025-05242-1","url":null,"abstract":"<p><strong>Purpose: </strong>This narrative review aims to provide an overview of current knowledge on mpox, emphasizing updated epidemiology and recent advances in treatment and prevention strategies, in light of the latest outbreaks.</p><p><strong>Methods: </strong>We searched PubMed and Google Scholar for publications on 'Mpox' and 'Monkeypox' up to June 5, 2025. Grey literature from governmental and health agencies was also accessed for outbreak reports and guidelines where published evidence was unavailable.</p><p><strong>Results: </strong>Recent outbreaks have redefined mpox epidemiology. Whereas previous regional outbreaks were mainly driven by zoonotic spillover with limited household transmission and often affecting children, more recent outbreaks have involved sustained human-to-human transmission. Such transmission has occurred among men who have sex with men for clade IIb and within heterosexual networks for clade Ib outbreaks, and more recently clade Ia outbreaks, primarily through sexual contact. Clinical features have also shifted toward more localized lesions, prominently in the anogenital area. While mpox is usually self-limited, severe cases may occur in pregnant women, young children, and immunocompromised individuals. Mpox management primarily relies on supportive care. In patients with severe mpox, or at risk of, tecovirimat was widely recognized as the first-line therapy, although it has failed to demonstrate its effectiveness in recent randomized controlled trials. The Modified Vaccinia Ankara vaccine (two-dose regimen) has shown a favorable safety profile and promising efficacy data in preventing clade IIb mpox, including immunocompromised individuals.</p><p><strong>Conclusion: </strong>Mpox has transitioned from a neglected zoonosis to a re-emerging global health threat. Sustained surveillance, robust and targeted public health interventions, and equitable access to diagnostics, vaccines, and antiviral treatments are critical to managing potential future mpox outbreaks.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Managing penicillin resistant pneumococcal meningitis: an international id-iri study.","authors":"Hakan Erdem, Elif Dogan, Handan Ankarali, Gorana Dragovac, Derya Seyman, Arzu Tarakci, Enes Dalmanoglu, Ayse Kaya-Kalem, Ayse Batirel, Seniha Senbayrak, Marija Cvetanovska, Sumeyye Kazancioglu, Abdullah Umut Pekok, Bilal Ahmad Rahimi, Mustafa Uguz, Selva Ala-Selek, Atahan Cagatay, Serap Demir-Tekol, Halil Kul, Tuba Kuruoglu, Fatih Temocin, Sevil Alkan, Furkan Baris Arikan, Turan Aslan, Meltem Tasbakan, Serife Altun-Demircan, Tuba Damar-Cakirca, Amani El-Kholy, Hasip Kahraman, Yordan Kalchev, Sholpan Kulzhanova, Anna Líšková, Serkan Oncu, Mehmet Özdemir, Deniz Özer, Nefise Öztoprak Cuvalci, Tugce Simsek-Bozok, Luca Mihaela Catalina, Egidia Miftode, Larisa Miftode, Marianna Murdjeva, Chizaram Onyeaghala, Bahar Busra Sivrikaya, Selcen Ozer-Kokkizil, Aysun Yalci, Oktay Yapici, Joanna Zajkowska, Teresa Fasciana","doi":"10.1007/s10096-025-05240-3","DOIUrl":"https://doi.org/10.1007/s10096-025-05240-3","url":null,"abstract":"<p><p>Penicillin-resistant pneumococcal meningitis (PRPM) is a challenging and fatal infection. We conducted a multicentre international retrospective study to evaluate the clinical features, outcomes, predictors of outcomes antimicrobial efficacy and drug susceptibility in patients with PRPM. The study, conducted through the \"Infectious Diseases-International Research Initiative\" across 33 centers in 11 countries, analyzed PRPM patients treated between 2019 and 2024 using univariate and multivariate analyses. A total of 138 patients were included. Of these, 83 (60.1%) were fully cured, 27 (19.6%) died, and 28 (20.3%) survived with sequelae. Mortality was associated with ICU admission (OR 14.886; p = 0.021), mechanical ventilation (OR 7.205; p = 0.049), and vasopressor use (OR 8.983; p = 0.025). Higher CSF leukocyte count (OR 0.854; p = 0.060) and blood leukocyte count (OR 0.283; p = 0.021) were linked to lower mortality risk. Patients who developed sequelae were more likely to require mechanical ventilation (OR 9.354; p = 0.001), experience recurrent meningitis (OR 5.562; p = 0.081), and have lower platelet counts (OR 0.001; p = 0.050), compared to those who fully recovered. Sequelae patients had higher GCS scores (OR 1.365; p = 0.014), more corticosteroid use (OR 5.301; p = 0.061), and less vasopressor use (OR 0.205; p = 0.019) compared to those who died. The antibiotic susceptibility profiles of the isolates in our PRSP cohort were: Ceftriaxone (75/134, 55.9%), meropenem (26/44, 59%), moxifloxacin (47/48, 97.9%). PRPM is a fatal disease in which mortality and sequelae occurring in two-fifths of cases. Severe illness markers such as ICU admission, mechanical ventilation, and vasopressor use, along with recurrent meningitis are linked to worse outcomes. Thrombocytopenia, low leukocyte counts, and lower GCS scores are indicators of poor prognosis, while corticosteroid therapy appears protective in PRPM. Therapeutic optimization is challenged by rising resistance and pharmacokinetic limitations, though moxifloxacin shows the highest susceptibility; further research is warranted.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of a novel DiaRD-HCV RNA RTqPCR kit for quantification of HCV RNA in clinical samples.","authors":"Ulker Cuhaci, Bengul Durmaz, Hande Toptan, Mehmet Koroglu, Derya Mutlu, Dilek Colak, Rıza Durmaz","doi":"10.1007/s10096-025-05179-5","DOIUrl":"10.1007/s10096-025-05179-5","url":null,"abstract":"<p><strong>Purpose: </strong>Quantitative detection of hepatitis C virus (HCV) RNA is essential for diagnosis of chronic infection and monitoring the HCV levels in patients under treatment. In this study, we aimed to evaluate the performance characteristics and comparability of a novel DiaRD-HCV RT qPCR kit (Diagen Lts, Ankara, Turkey) with Artus HCV QS-RGQ kit and the NeuMoDx HCV Quant Test in clinical samples.</p><p><strong>Methods: </strong>A total of 240 plasma samples, 86 HCV RNA positive and 154 negative, were used for comparison. Intra-assay and inter-assay reproducibility tests of the novel kit were carried out on clinical samples having HCV RNA loads of 1.42 × 10³ IU/µl, 1.40 × 10⁴ IU/ml, and 5.10 × 10⁵ IU/ml. HCV genotypes 1b, 2a, 2b, 3a, 4, 5, and 6 were tested by the novel system.</p><p><strong>Results: </strong>DiaRD HCV RTqPCR kit demonstrated a sensitivity of 98.8%, a specificity of 100%, and an accuracy of 99.6%. Linear regression analysis demonstrated strong correlation (R² = 0.915) on paired quantitative results. There was a good agreement between the novel kit and two comparator kits, with an overall mean difference of -0.12 log10 IU/ml obtained by Bland-Altman analysis. According to different viral loads, the intra-assay, inter-assay, and inter-load coefficients of variations (CVs) of the novel kit ranged from 5.31% to 11.73%, from 9.31% to 17.16%, and from 4.25% to 15.38% repeats. P-values were between 0.3 and 0.9, confirming no significant variations in the quantitative results of the reproducibility experiments. All genotypes tested were detected and quantitated with the mean differences between 0.01 and 0.62 log10 IU/ml. The primers and probe used in the novel kit did not result in any false-positive results with other pathogens.</p><p><strong>Conclusions: </strong>These results highlight the potential utility of the DiaRD HCV RTqPCR kit for quantitation and monitoring of HCV RNA in patients.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"2105-2117"},"PeriodicalIF":3.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}