Fast and visual RT-LAMP assay for detection of oropouche virus.

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2025-05-22 DOI:10.1007/s10096-025-05174-w

Livia do Carmo Silva, Diego Michel Fernandes Da Silva, Igor Marques Cesario Calassa, Juliana Santana De Curcio, Luiz Henrique Alves Costa, Flávia Barreto de Sousa, Carlos Eduardo Anunciação, Elisângela De Paula Silveira-Lacerda

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引用次数: 0

Abstract

Background: The epidemic potential of Oropouche virus (OROV) highlights the urgent need for rapid and accessible diagnostic methods to improve testing capacity and reduce result turnaround times, especially in tropical regions where OROV prevalence is rising.

Objective: To develop and validate a rapid, cost-effective diagnostic assay for OROV detection suitable for use in resource-limited and field settings.

Methods: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was designed and optimized for OROV detection. The assay's performance was evaluated in terms of sensitivity, specificity, time to result, and ease of visual interpretation based on colorimetric changes.

Results: The RT-LAMP assay produced results within 30 to 35 minutes and enabled straightforward visual detection through a clear color change. Its detection limit was comparable to that of real-time PCR, the current gold standard for molecular diagnosis. The assay's simplicity and minimal equipment requirements make it well-suited for decentralized testing environments.

Conclusions: This RT-LAMP assay represents a rapid, sensitive, and accessible tool for OROV diagnosis, with significant potential to enhance outbreak response and surveillance efforts in endemic regions.

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快速、直观RT-LAMP法检测口腔病毒。

背景：Oropouche病毒（OROV）的流行潜力突出表明迫切需要快速和可获得的诊断方法，以提高检测能力并缩短结果周转时间，特别是在OROV流行率上升的热带地区。目的：开发并验证一种适用于资源有限和现场环境的快速、具有成本效益的OROV检测诊断方法。方法：设计并优化逆转录环介导等温扩增（RT-LAMP）检测OROV的方法。该分析的性能在灵敏度、特异性、结果时间和基于比色变化的视觉解释的便利性方面进行了评估。结果：RT-LAMP法在30至35分钟内产生结果，并通过清晰的颜色变化实现直观的视觉检测。其检出限与实时PCR相当，实时PCR是目前分子诊断的金标准。该分析的简单性和最小的设备要求使其非常适合分散的测试环境。结论：该RT-LAMP检测方法是一种快速、敏感和易于获得的OROV诊断工具，具有显著的潜力，可以加强流行地区的疫情应对和监测工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.