Environmental Technology & Innovation最新文献

{"title":"Posidonia oceanica beach casts revalorization through high-boiling point organosolv solvents","authors":"Valeria Greyer,&nbsp;Ana Moral","doi":"10.1016/j.eti.2025.104102","DOIUrl":"10.1016/j.eti.2025.104102","url":null,"abstract":"<div><div>The accumulation of marine plants like <em>Posidonia oceanica</em> on beaches poses ecological challenges but also represents a renewable biomass resource. This study explores the potential of <em>Posidonia oceanica</em> as an alternative source of cellulose and lignin through an organosolv process, using non-conventional high-boiling point solvents such as ethyleneglycol, diethyleneglycol, ethanolamine and diethanolamine. Three operating conditions were tested: minimum (60 % solvent concentration, 160°C), medium (70 % solvent concentration, 170°C), and maximum (80 % solvent concentration, 180°C). The leaves treated with amine-based solvents under maximum conditions exhibited high cellulose purity, viscosity, and delignification, while glycolic solvents produced pulps with higher residual lignin, suitable for high-strength applications like corrugated cardboard. Compared to other raw materials, <em>Posidonia oceanica</em> pulps displayed greater viscosity and Kappa number values, positioning it as a competitive cellulose source. The alkanolamine treatments effectively fractionated the biomass into a cellulose-rich pulp and a lignin-rich black liquor, demonstrating the feasibility of utilizing <em>Posidonia oceanica</em> in a biorefinery process. The study highlights the ecological and economic advantages of utilizing marine biomass in a circular economy, reducing reliance on wood-based resources and mitigating deforestation, while providing value-added products like cellulose and lignin.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104102"},"PeriodicalIF":6.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocatalyst application of newly isolated Enterococcus species using microbial fuel cell (MFC) in wastewater treatment","authors":"Getachew Bantihun ,&nbsp;Andualem Mekonnen ,&nbsp;Seid Mohammed ,&nbsp;Yilkal Dessie ,&nbsp;Leta Guta","doi":"10.1016/j.eti.2025.104090","DOIUrl":"10.1016/j.eti.2025.104090","url":null,"abstract":"<div><div><em>Potentially useful species, electrogenicbacteria are used as sustainable anode biocatalysts to produce green energy and in wastewater management. Here, this study aims to identify and characterize electrogenic bacteria from six wastewater and one soil samples. Double chambered microbial fuel cell (MFC) system was designed to develop anode biofilm. Bio-electrochemical property of electrogenic bacteria isolates (EBIs) were characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and observation of biofilm on the anode using scanning electron microscope (SEM). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and 16S rRNA gene sequencing was performed for identification. From a total of 72 biofilm forming EBIs, MALDI-TOF-MS identified 21 selected EBIS, including Aeromons, Bacillus, Citrobacter, Pseudomonas and Raoultella genus</em>. This is the first study to characterize Enterococcus species from real brewery sludge waste, using the 16S rRNA sequencing. Evolutionary history analysis, GC content (53.72 %) and sequence alignment profiles infer this isolate as the first time report with biocatalyst potential. The evolutionary history for EBIs was found to be Enterococcus species and designated as E. faecium strain BSE14. The isolate showed biologically stable for long term operation of MFC (0.46 ± 0.038 V) with chemical oxygen demand (COD) (95.4 ± 0.82 %), biological oxygen demand (BOD) (53.3 ± 0.531 %), total nitrogen (TN) (73.1 ± 0.674) and total phosphorus (TP) (44.8 ± 0.281). Voltage generation among unknown mixed and pure EBIs exhibited significant variation (df=6, p &lt; 0.05 and df=7, p &lt; 0.05, respectively). Finally, the EBIs contribute as a potential biocatalyst in energy generation and wastewater treatment applications.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104090"},"PeriodicalIF":6.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and stability of a reusable laccase-polymer hybrid film for the removal of bisphenol A in water","authors":"J. Lucas Vallejo ,&nbsp;Saul Vallejos ,&nbsp;Miriam Trigo-López ,&nbsp;José M. García ,&nbsp;María D. Busto","doi":"10.1016/j.eti.2025.104093","DOIUrl":"10.1016/j.eti.2025.104093","url":null,"abstract":"<div><div>Laccases are a group of versatile and green biocatalysts with high efficiency for the degradation of a wide variety of environmental pollutants. However, the feasibility of free laccase applications is hampered by enzyme inactivation due to exposure to denaturing conditions and difficult recovery, which limits reuse and continuous process design, resulting in high costs. Enzyme immobilization technology is a promising way of overcoming these drawbacks. In this work, laccases from two fungal sources, <em>Myceliophthora thermophila</em> and <em>Trametes versicolor</em>, were immobilized for the first time by covalent interaction (azo-linkages) on a film-shaped polyacrylic material. Optimal immobilization parameters were 10 U/mL enzyme load, 1 h and 25 ºC. The resultant immobilized laccases were characterized as a function of pH, temperature, and substrate concentrations (kinetic parameters) and compared with the free enzyme counterpart. The laccase-polymer hybrid (<em>M. thermophila</em> laccase) showed excellent operational and storage stability, retaining 89.8 % of the initial activity after 15 cycles of reuse and 10 days of storage at 4 ºC. Finally, the laccase-polymer films exhibited &gt; 90 % depletion of bisphenol A in water by a dual adsorption-catalysis mechanism. The adsorption capacity ranged from 61.4 % to 30.5 %. GC-MS analysis revealed the degradation products 5,5′-bis-[1-(4-(4-hydroxyphenyl)-1-methyl-ethyl]-biphenyl-2,2′-diol and 3- or 2-methyl-2,3-ditrobenzofuran, as adsorbed compounds, as well as non-degraded bisphenol A. The significance of this research lies in the demonstrated catalytic efficiency of a new, reusable and cost-effective hybrid material for the removal of phenolic contaminants in water. The findings suggest that laccase-polymer hybrids could be a very useful tool for sustainable wastewater treatment.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104093"},"PeriodicalIF":6.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of gaseous VOC/SVOC during softwood torrefaction under various technological conditions","authors":"Jana Růžičková ,&nbsp;Michal Šafář ,&nbsp;Karolina Slamová ,&nbsp;Helena Raclavská ,&nbsp;Marek Kucbel ,&nbsp;Barbora Švédová ,&nbsp;Dagmar Juchelková ,&nbsp;Hana Brťková ,&nbsp;Jarmila Drozdová","doi":"10.1016/j.eti.2025.104086","DOIUrl":"10.1016/j.eti.2025.104086","url":null,"abstract":"<div><div>Torrefaction is a technology that can contribute to improving the properties of softwood biomass used for energy purposes (combustion, pyrolysis). As a result of the thermal treatment, the chemical composition of major compounds of biomass is changed, creating new compounds that were not contained in the original biomass. The article deals with the occurrence of organic compounds that are considered precursors of secondary organic aerosols (SOA) in gaseous emissions under different conditions of torrefaction (temperature, reaction time). Compounds considered precursors or intermediaries for the formation of secondary organic aerosol (SOA) have been identified based on data published in the literature. The concentration of SOA precursors in gaseous emissions decreases with increasing temperature of torrefaction. The mass balance shows that the gaseous component containing compounds that are considered by SOA precursors forms 6 % at torrefaction operated at 300 °C, 13 % at 250 °C, and up to 16.6 % at 200 °C. A statistically significant inversely proportional relationship between the amount of SOA emitted and the concentration of fixed carbon in the torrefied biomass has been demonstrated. With increasing concentrations of fixed carbon in the torrefied biomass, concentrations of emitted SOA precursors decrease. At 200 and 250 °C, SOA precursors are released at the highest concentrations and arise from the thermal conversion of hemicellulose. The lowest concentrations of SOA precursors released from hemicellulose and lignin were found at 300 °C. The article highlights the problem of SOA formation, which may affect not only human health but also climate change.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104086"},"PeriodicalIF":6.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Co3O4:SnO2 nanocomposite-modified GCE for sensitive and stable electrochemical detection of organophosphate pesticides","authors":"Mansoor Khan ,&nbsp;Shahid Ahmad ,&nbsp;Tahseen Kamal ,&nbsp;Sinan Sameer ,&nbsp;Sher Bahadar Khan","doi":"10.1016/j.eti.2025.104081","DOIUrl":"10.1016/j.eti.2025.104081","url":null,"abstract":"<div><div>Parathion methyl (PM) pesticide, widely used in agricultural practices, poses significant risks to human health and the environment due to its high toxicity and persistence in water and soil. Its detection at trace levels is critical for safeguarding public health and ensuring compliance with environmental regulations. This study presents the development of an electrochemical sensor for the detection of PM pesticide, utilizing a glassy carbon electrode (GCE) modified with Co₃O₄:SnO₂ nanocomposites in different ratios of 1:9, 2:8, and 3:7. The nanocomposites were extensively characterized using a range of techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS), which confirmed both the successful synthesis and the uniform distribution of the nanocomposites. To evaluate the electrochemical properties, cyclic voltammetry (CV) was employed, revealing that the Co₃O₄:SnO₂ (3:7) nanocomposite exhibited the highest oxidation peak current (Ipa) at pH 7, indicating superior catalytic activity for the sensing of PM. The electrochemical reaction was identified as an irreversible two-electron transfer process. Subsequent optimization of experimental conditions, such as pH, scan rate, and PM concentration, was conducted to maximize sensitivity, with pH 7 identified as the optimal condition. In addition, electrochemical impedance spectroscopy (EIS) was utilized to investigate the electron transfer dynamics at the electrode surface. The Nyquist plots showed a notable decrease in charge transfer resistance (Rct) for the Co₃O₄:SnO₂ (3:7) modified GCE, signifying improved electron transfer kinetics and faster response times compared to the bare GCE. This reduction in Rct contributed significantly to the enhanced electrochemical performance observed. Moreover, CV demonstrated excellent sensitivity (0.74 × 10⁻² mA mM⁻¹ cm⁻²). Additionally, differential pulse voltammetry (DPV) was used to calculate a low detection limit (LOD) of 0.015 mM and a limit of quantification (LOQ) of 0.045 mM for PM. To further assess the electrochemical behavior and sensitivity of the sensor, chronoamperometry showed excellent linearity over a wide concentration range. The recyclability and real sample studies were conducted using both CV and DPV techniques. Lastly, the coated Co₃O₄:SnO₂ (3:7) CGE are highly effective for the electrochemical detection of PM, offering superior sensitivity, stability, and reproducibility, making them promise for environmental monitoring and pesticide detection in agricultural water sources.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104081"},"PeriodicalIF":6.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oligotrophy biochar stimulates the generation of salicylic acid from soybean roots by increasing nutrient and oxidative stress","authors":"Pinyao Lan ,&nbsp;Quan Chen ,&nbsp;Min Wu ,&nbsp;Patryk Oleszczuk ,&nbsp;Bo Pan","doi":"10.1016/j.eti.2025.104083","DOIUrl":"10.1016/j.eti.2025.104083","url":null,"abstract":"<div><div>The accumulation of allelochemicals in farming land has attracted a great deal of research attention, and biochar has shown positive effects in alleviating allelopathy. This study investigated how oligotrophic biochar application modulated salicylic acid (SA) generation in soybean roots through nutrient and oxidative stress pathways. Biochars were applied to soybean cultivation, with analyses conducted on nutrient adsorption, allelochemical profiles, and plant growth parameters. Results revealed that biochar suppressed benzoic acid (BA) while elevating SA levels, which correlated with the presence of persistent free radicals (PFRs) and nutrient retention. The retention of phosphorus (P) and ammonium (NH₄⁺-N) dominated plant height reduction, surpassing oxidative stress effects linked to PFRs. Multivariate linear regression (MLR) identified P retention as the primary driver of SA generation, linked to adaptive phosphorus solubilization via acid secretion. Conversely, malondialdehyde (MDA) accumulation resulted from lipoxygenase-mediated lipid peroxidation under nutrient stress and PFRs-induced oxidative stress. The strong adsorption of P and nitrate (NO₃⁻-N) by biochar exacerbated soil oligotrophy, triggering SA overproduction as a stress compensation mechanism. The significant correlation between SA and MDA indicated bidirectional stress signaling, wherein allelochemicals exacerbate oxidative damage while activating defense responses. These findings emphasize the dual role of biochar as both a stress inducer and an allelopathy modulator, highlighting the necessity for optimizing pyrolysis and developing soil-specific strategies to balance agricultural benefits with ecological risks.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104083"},"PeriodicalIF":6.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of biomethane production during anaerobic digestion of chicken manure using a biocomposite hydrogel with iron-modified biochar","authors":"Bahare Salehi ,&nbsp;Lijun Wang ,&nbsp;Abolghasem Shahbazi","doi":"10.1016/j.eti.2025.104091","DOIUrl":"10.1016/j.eti.2025.104091","url":null,"abstract":"<div><div>A biocomposite hydrogel was developed by crosslinking cellulose, starch, and iron-modified biochar as an additive for enhancing anaerobic digestion (AD) of chicken manure (CM). The hydrogel had an iron content of 2.2 wt% and electrical conductivity of 0.73 mS/cm. The hydrogel showed high hydrophilicity and could adsorb water up to 63 % of its original dry mass. The results showed that the addition of a small amount of hydrogel in the AD could significantly increase the CH₄ yield and concentration, and decrease the CO₂ and H₂S contents in the biogas. The maximum methane yield and concentration during AD of CM at ∼8 % solid content and 55 °C were 275.13 mL/g VS and 63.3 %, which were achieved by adding the hydrogel at 0.66 wt% of the slurry. The yield and concentration of methane produced by the AD with 0.66 wt% hydrogel increased by 34.5 % and 7.7 %, compared to the control without the hydrogel, which produced 204.49 mL/g VS methane with 58.8 % CH₄ in the biogas over the 21 days. The addition of 0.66 wt% hydrogel could reduce 51.1 % of the H₂S in the biogas produced by the 21-day AD of CM, compared to the control without the hydrogel containing an average of 1243 ppm H₂S. Also, it was shown that the addition of the hydrogel formed by cross-linking activated biochar, CMC, and starch to AD at a concentration of 0.66 wt% increased the biomethane yield by 23.33 % compared with the addition of the same amounts of the non-crosslinked raw hydrogel ingredients.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104091"},"PeriodicalIF":6.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An accelerated kinetic leach test for geochemical and environmental characterisation of acid and metalliferous drainage","authors":"Annah Moyo ,&nbsp;Anita Parbhakar-Fox ,&nbsp;Sebastien Meffre ,&nbsp;David R. Cooke","doi":"10.1016/j.eti.2025.104092","DOIUrl":"10.1016/j.eti.2025.104092","url":null,"abstract":"<div><div>A new bench scale accelerated kinetic leach column (KLC) test was developed to assess the acid and metalliferous drainage (AMD) potential of six historic mine wastes from Tasmania, Australia. Preliminary geochemical static tests evaluated three of the samples as potentially acid-forming (PAF) and two as uncertain because they contain considerable amounts of both acid-neutralising and acid-generating minerals. Mineralogy-based AMD potential evaluated the &lt; 1 mm size fractions of the two samples as PAF. The follow-up accelerated KLC test conducted over 100 days showed that five samples had a 10-day lag phase followed by the onset of acid generation. The 10-day drying cycles from day 30 increased the leachate pH by ≤ 1.5 units possibly from the rapid precipitation of secondary minerals and encapsulation of sulfide minerals. The samples released metal(loid)s including Al, As, Cd, Cr, Cu, Pb, and Zn in concentrations posing moderate to extreme risk to freshwater throughout the test period. The leaching kinetics indicated that the mine waste samples can generate AMD for centuries, thus AMD management strategies at the mine sites may be necessary for environmental protection. A comparison of pH and water hazard indices showed that the accelerated KLC is a rapid geochemical and environmental screening tool between static tests and long-term KLC tests that can validate and complement conventional methods.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104092"},"PeriodicalIF":6.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of multi-stage inoculation on the efficiency of hyperthermophilic composting of cationic polyacrylamide-containing sludge","authors":"Tianwen Song ,&nbsp;Qi Meng ,&nbsp;Jiafeng Jin ,&nbsp;Haoshuai Li ,&nbsp;Shanshan Duan ,&nbsp;Wenxiang Xia ,&nbsp;Mike Zhou","doi":"10.1016/j.eti.2025.104085","DOIUrl":"10.1016/j.eti.2025.104085","url":null,"abstract":"<div><div>The scale of sewage treatment in China currently grows consistently, leading to a rapid increase in sewage sludge production. Cationic polyacrylamide (CPAM) is widely used to dewater sewage sludge. However, during land application and landfill processes of dewatered sludge, a considerable amount of CPAM is released into the environment, posing severe pollution risks. This study explored the effects of different inoculation strategies for <em>Bacillus licheniformis</em>, <em>Calditerricola satsumensis</em>, and <em>Bacillus subtilis</em> on the efficiency of hyperthermophilic composting (HTC) of CPAM-containing sludge. The results showed that multi-stage inoculation significantly enhanced the efficiency of HTC and removal rate of CPAM. Compared with the non-inoculation group, multi-stage inoculation extended the hyperthermophilic phase from 7 to 11 days, increased the maximum temperature from 82.06°C to 85.16°C, and increased the removal rate of CPAM from 72.54 % to 84.90 %. The analysis of seed germination index (GI) and fluorescence excitation-emission matrix (EEM) spectra both revealed that multi-stage inoculation promoted the decomposition and humification of organic matter. Illumina MiSeq sequencing indicated that multi-stage inoculation significantly improved the diversity and richness of the microbial communities. Firmicutes, Actinobacteriota, and Proteobacteria were the predominant bacterial phyla, while <em>Bacillus</em>, <em>Calditerricola</em>, and <em>Sporolactobacillus</em> were the key functional bacterial genera involved in CPAM degradation. To the best of our knowledge, this is the first study to enhance the removal efficiency of CPAM by HTC using a multi-stage inoculation approach. These findings provide valuable new strategies for the efficient treatment of CPAM-containing sludge.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104085"},"PeriodicalIF":6.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water sample stability","authors":"Lionel PINEAU ,&nbsp;Vanessa CORDIER","doi":"10.1016/j.eti.2025.104080","DOIUrl":"10.1016/j.eti.2025.104080","url":null,"abstract":"<div><div>Numerous guidelines have been issued on the conditions under which water samples should be transported and stored before microbiological analysis. These recommendations are often very restrictive regarding the maximum acceptable storage time (MAST) of samples before analysis and they generate operational constraints that have significant economic and environmental consequences (e.g. samples rejected, unjustified prolongation of quarantine of a water supply point or medical device, recontrol,…). Unfortunately, the relevance of these MAST has never been demonstrated. This study investigates bacterial flora evolution in water samples according to their storage time before analysis. The first part, conducted in laboratory conditions follows the concentration of microorganisms in artificially contaminated water samples. The second part involves a retrospective analysis of real water sample results to verify whether the results varied statistically according to the sample storage time before analysis. The results of the laboratory study confirm that the microorganism concentration in the artificially contaminated samples vary by less than 25 % during the storage time. The second part of the study demonstrates that the ratio of samples belonging to the different result groups (e.g. &lt; 1 CFU/ X mL, between 1 and 99 CFU/X mL and ≥ 100 CFU/100 mL) remains unchanged according to storage time (variation &lt; 1 %). These findings confirm that it is possible to increase the MAST defined in standards, up to 26 h or 28 h according to the microorganism considered (instead of 12 h or 18 h) without compromising the validity of microbiological analysis. These changes should limit the operational, environmental, and economic constraints associated with the actual MAST by reducing the number of samples rejected and consecutively the number of recontrols.</div></div>","PeriodicalId":11725,"journal":{"name":"Environmental Technology & Innovation","volume":"38 ","pages":"Article 104080"},"PeriodicalIF":6.7,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}